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Tissue References Search Tool

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Endothelial
Rat
Rat, Wistar, male, 3 mo
Nagelkenke, J. , Barto, K. and VanBerkel, T.
In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells ,
J Biol Chem
 258 , 12221 , 1983
Cells
Endothelial
Kupffer
Parenchymal
Enzymes
Collagenase : 0.05%
Pronase : 0.25%
Medium
HBSS
Epithelial
Rat
Rat, SD, male, 150 - 250 g
Dial, E. , Kao, Y. and Lichtenberger, L.
Effects of 16,16-Dimethyl Prostaglandin E2 On Glycoprotein And Lipid Synthesis of Gastric Epithelial Cells Grown in a Primary Culture ,
In Vitro Cell Dev Biol
 27 , 39 , 1991
Cells
Epithelial
Stomach
Enzymes
Pronase : 0.15%
Medium
Medium 199
Epithelial
Rat
Rat, Fisher 344, male, 8 wks old,
250-300 g
Chang, L. , Wu, R. and Nettesheim, P.
Morphological Changes in Rat Tracheal Cells During The Adaptive and Early Growth Phase in Primary Cell Culture ,
J Cell Sci
 74 , 283 , 1985
Cells
Tracheal epithelial
Enzymes
Pronase : 0.5%
Medium
DMEM
Heart
Rat
Rat, SD, 8-10 week
Lee, J. , Ha, J. and Leem, C.
A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca(2+) Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat. ,
Korean J Physiol Pharmacol
 19 , 373-82 , 2015
Cells
Ventricular myocytes
Enzymes
Collagenase : 0.08%
Pronase : 0.004%
Medium
Tyrode's Solution
Liver
Mouse
Mouse, 12 week
Mederacke, I. , Dapito, D. , Affo, S. , Uchinami, H. and Schwabe, R.
High-Yield and High-Purity Isolation of Hepatic Stellate Cells from Normal and Fibrotic Mouse Livers. ,
Nat Protoc
 10 , 305-15 , 2015
Cells
Hepatic stellate
Enzymes
Pronase : 0.04%
Medium
DMEM
Liver
Mouse
Mouse
Rountree, C. , Ding, W. , Dang, H. , Vankirk, C. and Crooks, G.
Isolation of CD133+ Liver Stem Cells for Clonal Expansion. ,
J Vis Exp
 56 , e3183 , 2011
Cells
CD133+ stem
Enzymes
Pronase : 0.05%
Medium
DMEM/F12
Liver
Rat
Rat, SD
Handy, J. , Saxena, N. , Fu, P. , Lin, S. , Mells, J. , Gupta, N. and Anania, F.
Adiponectin Activation of AMPK Disrupts Leptin-Mediated Hepatic Fibrosis via Suppressors of Cytokine Signaling (SOCS-3). ,
J Cell Biochem
 110 , 1195 , 2010
Cells
Hepatic stellate cells
Enzymes
Pronase : 0.02%
Collagenase : see reference
Medium
Medium 199
Liver
Rat
Rat, Wistar, female
Zvibel, I. , Atias, D. , Phillips, A. , Halpern, Z. and Oren, R.
Thyroid Hormones Induce Activation of Rat Hepatic Stellate Cells Through Increased Expression of p75 Neurotrophin Receptor and Direct Activation of Rho. ,
Lab Invest
 90 , 674 , 2010
Cells
Hepatic stellate cells
Enzymes
Collagenase Type 1 : 0.025-0.1%
Pronase : 0.025-0.13%
Medium
DMEM
Liver
Rat
Rat, SD, 500-750 g
Li, Z. , Dranoff, J. , Chan, E. , Uemura, M. and Sevigny, J.and Wells, R.
Transforming Growth Factor-beta and Substrate Stiffness Regulate Portal Fibroblast Activation in Culture. ,
Hepatol
 46 , 1246-56 , 2007
Cells
Portal fibroblasts
Enzymes
Collagenase : 0.03%
Pronase : 0.033%
Hyaluronidase : 0.036%
Medium
DMEM/F-12
Liver
Rat
Rat, adult
Jensen C. , Jauho E. , Santoni-Rugiu E. , Holmskov U. , Teisner B. , Tygstrup N. and Bisgaard H.
Transit-amplifying Ductular (oval) Cells and Their Hepatocytic Progeny are Characterized by a Novel and Distinctive Expression of Delta-like Protein/preadipocyte Factor 1/fetal Antigen 1 ,
Am J Pathol
 164(4) , 1347 , 2004
Cells
Hepatocytes
Enzymes
Pronase : 1%
Medium
Williams E
Liver
Rat
Rat, CD strain, albino, male, 140 -180 g
Braun, L. , Mead, J. , Panzica, M. , Mikumo, R. , Bell, G. and Fausto, N.
Transforming Growth Factor B mRNA Increases During Liver Regeneration:A Possible Paracrine Mechanism Of Growth Regulation ,
Proc Natl Acad Sci U S A
 85 , 1539 , 1988
Cells
Hepatocytes and Nonparenchymal
Enzymes
Pronase : 0.1%
Medium
HEPES buffer with calcium
Liver
Rat
Rat, Wistar, male, 3 month old
Nagelkenke, J. , Barto, K. and VanBerkel, T.
In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells ,
J Biol Chem
 258 , 12221 , 1983
Cells
Endothelial
Kupffer
Parenchymal
Enzymes
Collagenase : 0.05%
Pronase : 0.25%
Medium
HBSS
Mammary
Mouse
Mouse
Boulanger CA, Smith GH.
Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
 20 , 2264-72 , 2001
Cells
Mammary epithelial stem
Enzymes
Hyaluronidase : 0.1%
Pronase : 1.25%
Medium
DMEM
Mammary
Mouse
Mouse (BALB/cCrgl), mature
Bandyopadhyay, G. , Imagawa, W. , Wallace, D. and Nandi, S.
Proliferative effects of insulin and epidermal growth factor on mouse mammary epithelial cells in primary culture ,
J Biol Chem
 263 , 7567 , 1988
Cells
Epithelial
Enzymes
Pronase : 0.01%
Medium
Hepes buffered medium 199
Muscle
Mouse
Mouse, 10 week
Rezk, B. , Yoshida, T. , Semprun-Prieto, L. , Higashi, Y. , Sukhanov, S. and Delafontaine, P.
Angiotensin II Infusion Induces Marked Diaphragmatic Skeletal Muscle Atrophy. ,
PLoS ONE
 7 , e30276 , 2012
Cells
Diaphragm cells
Enzymes
Collagenase Type 2 : 100 u/ml
Pronase : 0.125%
Medium
PBS
Stem
Mouse
Mouse
Rountree, C. , Ding, W. , Dang, H. , Vankirk, C. and Crooks, G.
Isolation of CD133+ Liver Stem Cells for Clonal Expansion. ,
J Vis Exp
 56 , e3183 , 2011
Cells
CD133+ stem
Enzymes
Pronase : 0.05%
Medium
DMEM/F12
Stem
Mouse
Mouse
Boulanger CA, Smith GH.
Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
 20 , 2264-72 , 2001
Cells
Mammary epithelial stem
Enzymes
Hyaluronidase : 0.1%
Pronase : 1.25%
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.