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Tissue References Search Tool

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Miscellaneous
Canine

Canine, Rat, Rabbit, Ovine, Bovine, Human

Basatvat, S. , Bach, F. , Barcellona, M. , Binch, A. , Buckley, C. , Bueno, B. , Chahine, N. , Chee, A. and Maitre, C.
Harmonization and Standardization of Nucleus Pulposus Cell Extraction and Culture Methods. ,
Spine
 6 , e1238 , 2023
Cells
nucleus pulposus
Enzymes
Pronase : 0.075%
Medium
DMEM
Miscellaneous
Human
Human
Sun, Y. , Lv, M. , Zhou, L. , Tam, V. , Lv, F. , Chan, D. , Wang, H. , Zheng, Z. , Cheung, K. and Leung, V.
Enrichment of Committed Human Nucleus Pulposus Cells Expressing Chondroitin Sulfate Proteoglycans under Alginate Encapsulation. ,
Osteoarthritis Cartilage
 23 , 1194-203 , 2015
Cells
Nucleus pulposus
Enzymes
Pronase : 0.25%
Collagenase Type 2 : 600 u/ml
Medium
DMEM
Miscellaneous
Porcine
Porcine, <10 week
Potier, E. and Ito. K.
Can Notochordal Cells Promote Bone Marrow Stromal Cell Potential for Nucleus Pulposus Enrichment? A Simplified in Vitro System. ,
Tissue Eng Part A
 20 , 3241-51 , 2014
Cells
Nucleus pulposus
Enzymes
Collagenase : 0.05%
Pronase : 0.3%
Medium
DMEM
Miscellaneous
Porcine
Porcine, various ages
Cho, H. , Park, S. , Lee, S. , Kang, M. , Hasty, K. and Kim, S.
Snapshot of Degenerative Aging of Porcine Intervertebral Disc: A Model to Unravel the Molecular Mechanisms. ,
Exp Mol Med
 43 , 334-40 , 2011
Cells
Nucleous pulposus and AF
Enzymes
Pronase : 0.05%
Collagenase : 0.2%
Medium
Modified F-12K
Organoids
Human

Human

Vaart, J. , Böttinger, L. , Geurts, M. , Wetering, W. , Knoops, K. , Sachs, N. , Begthel, H. , Korving, J. , Lopez-Iglesias, C. , Peters, P. , Eitan, K. , Gileles-Hillel, A. and Clevers, H.
Modelling of Primary Ciliary Dyskinesia using Patient-derived Airway Organoids. , EMBO Rep Vol. 22 , e52058 , 2021
Cells
Airway organoids
Enzymes
Pronase : 0.15%
Collagenase : 0.05%
Medium
Airway medium

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.