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Tissue References Search Tool

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Epithelial
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Eye
Mouse
Mouse
Schmidt, T. and Kofuji, P.
An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells. ,
J Vis Exp
 47 , 2367 , 2011
Cells
Retinal ganglion
Enzymes
CLSPA : 240 u/ml
Hyaluronidase : 0.2%
Medium
Ames
Heart
Mouse

Mouse, neonatal

Angelini, A. , Pi, X. and Xie, L.
Evaluation of Long-Chain Fatty Acid Respiration in Neonatal Mouse Cardiomyocytes using SeaHorse Instrument. ,
Star Protocols
 3 , 101392 , 2022
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.05%
CLSPA : 150 u/ml
Medium
L-15
Heart
Mouse
Mouse, male, 12-18 week
Jian, Z, Chen, Y, Shimkunas, R, Jian, Y, Jaradeh, M, Chavez, K, Chiamvimonvat, N, Tardiff, J, Izu, L, Ross, R. and Chen-Izu, Y
In Vivo Cannulation Methods for Cardiomyocytes Isolation from Heart Disease Models. ,
PLoS ONE
 11 , e0160605 , 2016
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 300 u/ml
Protease XIV : 0.004%
Medium
Perfusion solution (see reference)
Heart
Mouse

Mouse, 8-12 week

Ackers-Johnson, M. , Li, P. , Holmes, A. , O'Brien, S. , Pavlovic, D. and Foo, R.
A Simplified, Langendorff-Free Method for Concomitant Isolation of Viable Cardiac Myocytes and Nonmyocytes From the Adult Mouse Heart. ,
Circ Res
 119 , 909-20 , 2016
Cells
Cardiomyocytes
Enzymes
Protease XIV : 0.005%
Medium
Perfusion buffer (see reference)
Heart
Mouse
Mouse, adult, 9-13 week
Kabaeva, Z. , Zhao, M. and Michele, D.
Blebbistatin Extends Culture Life of Adult Mouse Cardiac Myocytes and Allows Efficient and Stable Transgene Expression. ,
Am J Physiol Heart Circ Physiol
 294 , H1667-74 , 2008
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 620 u/ml
Protease XIV : 0.104 u/ml
Medium
Myocyte buffer see reference
Heart
Mouse
Mouse, adult, 6-12 week
Shioya, T.
A Simple Technique for Isolating Healthy Heart Cells from Mouse Models. ,
J Physiol Sci
 57 , 327-35 , 2007
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.006%
Protease XIV : 0.006%
Medium
see reference
Heart
Mouse
Mouse, 2-4 mo
Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP.
Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology ,
Am J Physiol Heart Circ Physiol
 279 , H429-36 , 2000
Cells
Ventricular myocytes
Enzymes
Protease XIV : 0.002%
Medium
MEM
Mammary
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Pancreas
Mouse
Mouse, adult, male
Ji, B. , Gaiser, S. , Chen, X. , Ernst, S. and Logsdon, C.
Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation. ,
J Biol Chem
 284 , 17488 , 2009
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Mouse
Mouse, 6-10 month
Wang, Y. , Soyombo, A. , Shcheynikov, N. , Zeng, W. , Dorwart, M. , Marino, C. , Thomas, P. and Muallem, S.
Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis. ,
EMBO J
 25 , 5049 , 2006
Cells
Pancreatic ductal
Enzymes
CLSPA : 50 u/ml
Hyaluronidase : 400 u/ml
Medium
DMEM
Pancreas
Mouse
Mouse, adult
Voronina, S. , Barrow, S. , Gerasimenko, O. , Petersen, O. and Tepikin, A.
Effects of Secretagogues and Bile Acids on Mitochondrial Membrane Potential of Pancreatic Acinar Cells: Comparison of Different Modes of Evaluating Delta Psi. ,
J Biol Chem
 279 , 27327 , 2004
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
see reference
Pancreas
Mouse
Mouse, albino, 25 g
Fogarty, K. , Kidd, J. , Tuft, R. and Thorn, P.
A Bimodal Pattern of InsP(3)-Evoked Elementary Ca(2+) Signals in Pancreatic Acinar Cells. ,
Biophys J
 78 , 2298 , 2000
Cells
Acinar
Enzymes
CLSPA : see reference
Medium
see reference
Pancreas
Mouse
Mouse
Toivola, D. , Ku, N. , Ghori, N. , Lowe, A. , Michie, S. and Omary, M.
Effects of Keratin Filament Disruption on Exocrine Pancreas-Stimulated Secretion and Susceptibility to Injury. ,
Exp Cell Res
 255 , 156 , 2000
Cells
Acinar cells and acini
Enzymes
Collagenase Type 1 : see reference
CLSPA : see reference
Medium
see reference
Pancreas
Mouse
Mouse, male, 18-24 g
Burnham DB, Williams JA.
Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini ,
J Biol Chem
 257 , 10523-8 , 1982
Cells
Acinar
Enzymes
CLSPA : 70-90 u/ml
Medium
Krebs-Henseleit

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.