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Tissue References Search Tool

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Liver
Rat
Rat, Fischer, male, 150-250 g
Francavilla, A. , Ove, P. , Polimeno, L. , Sciascia, C. , Coetzee, M. and Starzi, T.
Epidermal Growth Factor and Proliferation in Rat Hepatocytes in Primary Culture Isolation at Different Times after Partial Hepatectomy ,
Cancer Res
 46 , 1318 , 1986
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 125 - 250 u/ml
Medium
MEM
(see reference)
Liver
Rat
Rat, SD, female, 100-180 g
Schwarz, K. , Lanier, S. , Carter, E. , Homcy, C. and Graham, R.
Rapid Reciprocal Changes in Adrenergic Receptors in Intact Isolated Hepatocytes During Primary Cell Culture ,
Mol Pharmacol
 27 , 200 , 1985
Cells
Hepatocytes
Enzymes
Collagenase : 0.5%
Medium
Krebs Ringer bicarbonate buffer
Liver
Rat
Rat, Lewis, female, 200-250 g
Holstege A, Leser HG, Pausch J, Gerok W.
Uridine catabolism in Kupffer cells, endothelial cells, and hepatocytes ,
Eur J Biochem
 149 , 169-73 , 1985
Cells
Hepatocytes, Kupffer, endothelial
Enzymes
Collagenase : 0.05%
Medium
Gey's BSS
Liver
Rat
Rat, SD, male, 270 - 320 g
Brass, E. , Garrity, M. and Robertson, R.
Inhibition of Glucagon-Stimulated Hepatic Glycogenolysis by E-Series Prostaglandins ,
F.E.B.S. Lett.
 169 , 293 , 1984
Cells
Hepatocytes
Enzymes
Collagenase : 0.04%
Medium
Bicarbonate buffer with calcium added
Liver
Rat
Rat, Wistar, male, 3 month old
Nagelkenke, J. , Barto, K. and VanBerkel, T.
In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells ,
J Biol Chem
 258 , 12221 , 1983
Cells
Endothelial
Kupffer
Parenchymal
Enzymes
Collagenase : 0.05%
Pronase : 0.25%
Medium
HBSS
Liver
Rat
Rat, SD, male/female, 250-300 g
Studer, R. and Borle, A.
Differences between Male and Female Rats in the Regulation of Hepatic Glycogenolysis. The Relative Role of Calcium and cAMP in Phosphorylase Activation by Catecholamines ,
J Biol Chem
 257 , 7987 , 1982
Cells
Hepatocytes
Enzymes
Collagenase : 100 u/ml
Medium
Krebs Henseleit bicarbonate buffer
Liver
Rat
Rat, SD
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 90 u/ml
Medium
CF EGTA perfusate
Liver
Rat
Rat, Zucker, obese
Goldstein, A. , Palmer, J. and Johnson, P.
Primary Cultures of Fetal Hepatocytes from the Genetically Obese Zucker Rat: Protein Synthesis ,
In Vitro
 17 , 651 , 1981
Cells
Hepatocytes
Enzymes
Medium
Dulbecco-Vogt arginine free Eagle's
Liver
Rat
Rat, Wistar, female, fetus
Gugen-Guillouzo, C. , Tichonicky, L. , Szajnert, M. and Kruh, J.
Changes in Some Chromatin and Cytoplastic Enzymes of Perinatal Rat Hepatocytes ,
In Vitro
 16 , 1 , 1980
Cells
Hepatocytes
Enzymes
Collagenase : 0.025%
Medium
HEPES buffer
Liver
Rat
Rat, SD, 7-10 day
Acosta, D. , Anuforo, D. and Smith, R
Preparation of Primary Monolayer Cultures of Postnatal Rat Liver Cells ,
J Tiss Cul Meth
 6 , 35 , 1980
Cells
Liver
Enzymes
Collagenase : 0.05%
Medium
HBSS modified (see reference)
Liver
Rat
Rat, SD, male, 250-300 g
Yamada, S. , Otto, P. , Kennedy, D. and Whayne, T.
The Effects of Dexamethasone on Metabolic Activity of Hepatocytes in Primary Monolayer Culture ,
In Vitro
 16 , 559 , 1980
Cells
Parenchymal
Enzymes
Medium
Kreb's Henseleit bicarbonate buffer
Liver
Rat
Rat, SD, female, 100-150 g
Davis, R. , Engelhorn, S. , Pangburn, S. , Weinstein, D. and Steinberg, D.
Very Low Density Lipoprotein Synthesis and Secretion by Cultured Rat Hepatocytes ,
J Biol Chem
 254 (6) , 2010 , 1979
Cells
Hepatocytes
Enzymes
Medium
DMEM
(see reference)
Liver
Rat
Rat
(also chicken)
Weigel, P. , Schnaar, R. , Kuhlenschmidt, M. , Schmell, E. , Lee, R. , Lee, Y. and Roseman, S.
Adhesion of Hepatocytes to Immobilized Sugars ,
J Biol Chem
 254 (21) , 10830 , 1979
Cells
Hepatocytes
Enzymes
Collagenase Type 4 : 6000 units
Medium
HEPES
Liver
Rat
Rat , Wistar, male, 250-300 g
Gravela, E. , Poli, G. , Albano, E. and Dianzani, M.
Studies of Fatty Liver with Isolated Hepatocytes ,
Exp Mol Pathol
 27 , 339 , 1977
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
Hank's solution, CF
Liver
Rat
Rat, SD, male, 200 g
Rubin, K. , Kjellen, L. and Oslashbrink, B.
Intercellular Adhesion between Juvenile Liver Cells.. A Method to Measure the Formation of Stable Lateral Contacts Between Cells Attached to a Collagen Gel ,
Exp Cell Res
 109 , 413 , 1977
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 100 u/ml
Medium
Buffers 1 & 2
(see reference)
Liver
Rat
Rat (WAG/RIJ), female, 24, 30, and 37 months
VanBezodijen, C. , Grell, T. and Knook, D.
Effect of Age on Protein Synthesis by Isolated Liver Parenchymal Cells ,
Mech Ageing Dev
 6 , 293 , 1977
Cells
Parenchymal
Enzymes
Collagenase Type 1 : 0.05% - 0.06%
Medium
HEPES buffer
Liver
Rat
Rat, Fisher, adult, male
Williams, G. , Bermudez, E. and Scaramuzzino, D.
Rat Hepatocytes Primary Cell Cultures III. Improved Dissociation and Attachment Techniques and the Enchancement of Survival by Culture Medium ,
In Vitro
 13 (12) , 809 , 1977
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 100 u/ml
Medium
HBSS
(see reference)
Liver
Rat
Rat, Fischer, male, adult, 170-265 g
Laishes, B. and Williams, G.
Conditions Affecting Primary Cell Cultures of Functional Adult Rat Hepatocytes. I.. The Effect of Insulin ,
In Vitro
 12 , 521 , 1976
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%-0.10%
Medium
Williams E
Liver
Rat
Rat, SD, male, 200-250 g
Witters, L. , Alberico, L. and Acruch, J.
Insulin Regulation of Glycogen Synthase in the Isolated Rat Hepatocyte ,
Biochem Biophys Res Commun
 69 (4) , 997 , 1976
Cells
Hepatocytes
Enzymes
Medium
MEM
Liver
Rat
Rat, SD, adult, male, 180-300 g
Bissell, D. , Hammaker, L. and Meyer, U.
Parenchymal Cells from Adult Rat Liver in Nonproliferating Monolayer Culture. I. Functional Studies ,
J Cell Biol
 59 , 722 , 1973
Cells
Parenchymal
Enzymes
Medium
Hank's solution, CF
Liver
Rat
Rat, Wistar, male, 260-310 g
Seglen, P.
Preparation of Rat Liver Cells ,
Exp Cell Res
 74 , 450 , 1972
Cells
Hepatocytes
Enzymes
Collagenase : 0.01 - 0.08%
Medium
HEPES
Liver
Rat
Rat, Fisher 344, pregnant (19-21 days gestation),
200-250 g
Leffert, H. and Paul, D.
Studies on Primary Cultures of Differentiated Fetal Liver Cells ,
J Cell Biol
 52 , 559 , 1972
Cells
Hepatocytes
Enzymes
Collagenase : 0.3%
Medium
Modified Eagle's w/ Serum
Liver
Rat
Rat, SD, adult, 200-300 g
Berry, M. and Friend, D.
High Yield Preparation of Isolated Rat Liver Parenchymal Cells ,
J Cell Biol
 43 , 506 , 1969
Cells
Parenchymal
Enzymes
Medium
HBSS, CF
Lung
Bovine
Bovine
Del Vecchio, P. , Siflinger-Birnboim, A. , Belloni, P. , Holleran, L. , Lum, H. and Malik, A.
Culture and Characterization of Pulmonary Microvascular Endothelial Cells ,
In Vitro Cell Dev Biol
 28A , 711 , 1992
Cells
Endothelial
Pulmonary
Enzymes
Collagenase : 1000 u/ml
Collagenase Type 2 : 1000 u/ml
Medium
PBS, CMF
Lung
Human

Human

Konishi, S. , Tata, A. and Tata, P.
Defined Conditions for Long-term Expansion of Murine and Human Alveolar Epithelial Stem Cells in Three-dimensional Cultures. , STAR Protoc Vol. 3 , 101447 , 2022
Cells
Alveolar epithelial stem
Enzymes
Collagenase Type 1 : 450 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Human

Human

Youk, J. , Kim, T. , Evans, K. , Jeong, Y. , Hur, Y. , Hong, S. , Kim, J. , Yi, K. , Kim, S. , Na, K. , Bleazard, T. , Kim, H. , Fellows, M. , Mahbubani, K. , Saeb-Parsy, K. , Kim, S. , Kim, Y. , Koh, G. , Choi, B. , Ju, Y. and Lee, J.
Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2. , Cell Stem Cell Vol. 27 , 905-919.e10 , 2020
Cells
Aveolar
Enzymes
Medium
DMEM
Lung
Human
Human
Quatromoni, J. , Singhal, S. , Bhojnagarwala, P. , Hancock, W. , Albelda, S. and Eruslanov, E.
An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells ,
J Leukoc Biol
 97 , 201 , 2015
Cells
Lung tumor
Enzymes
Collagenase Type 1 : 45-60 u/ml
Collagenase Type 2 : 15-20 u/ml
Collagenase Type 4 : 45-60 u/ml
Elastase : 0.002%
Medium
DMEM/F12
Lung
Human
Human
Mackay, L. , Dodd, S. , Dougall, I. , Tomlinson, W. , Lordan, J. , Fisher, A. and Corris, P.
Isolation and Characterisation of Human Pulmonary Microvascular Endothelial Cells from Patients with Severe Emphysema. ,
Respir Res
 14 , 23 , 2013
Cells
Pulmonary endothelial
Enzymes
Medium
RPMI
Lung
Human
Human
Comhair, S. , Xu, W. , Mavrakis, L. , Aldred, M. , Asosingh, K. and Erzurum, S.
Human Primary Lung Endothelial Cells in Culture. ,
Am J Respir Cell Mol Biol
 46 , 723-30 , 2012
Cells
Lung endothelial
Enzymes
Medium
PBS
Lung
Human
Human
Fujino, N. , Kubo, H. , Suzuki, T. , Ota, C. , Hegab, A. , He, M. , Suzuki, S. , Suzuki, T. , Yamada, M. , Kondo, T. , Kato, H. and Yamaya, M.
Isolation of Alveolar Epithelial Type II Progenitor Cells from Adult Human Lungs. ,
Lab Invest
 91 , 363 , 2011
Cells
Lung
Enzymes
Medium
DMEM
Lung
Human
Human fetuses, 80 day
(also swine fetuses, 70 day, adult Amer Dutch, 250 day)
Hinz, R. and Syverton, J.
Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase ,
Proc Soc Exp Biol Med
 101 , 19 , 1959
Cells
Lung
Enzymes
Collagenase : 0.01%
Medium
HBSS
Lung
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Lung
Mouse

Mouse

Horn, K. , Fulte, S. , Yang, M. , Lorenz, B. and Clark, S.
Neutrophil Responsiveness to IL-10 Impairs Clearance of Streptococcus pneumoniae from the Lungs. ,
J Leukoc Biol
 , 2023
Cells
Lung
Enzymes
Medium
PBS
Lung
Mouse

Mouse

Konishi, S. , Tata, A. and Tata, P.
Defined Conditions for Long-term Expansion of Murine and Human Alveolar Epithelial Stem Cells in Three-dimensional Cultures. , STAR Protoc Vol. 3 , 101447 , 2022
Cells
Alveolar epithelial stem
Enzymes
Collagenase Type 1 : 450 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Mouse
Mouse
Qiu, Y. , Xu, Y. , Wang, M. , Miao, M. , Zhou, H. , Xu, J. , Kong, J. , Zheng, D. , Li, R. , Zhang, R. , Guo, Y. , Li, X. , Cui, J. , Qin, C. and Zhou , X.
Flavivirus Induces and Antagonizes Antiviral RNA Interference in Both Mammals and Mosquitoes. ,
Sci. Adv.
 6 , 7989 , 2020
Cells
Fibroblasts
Enzymes
Medium
HBSS
Lung
Mouse
2-3 month
Wang, J. , Niu, N. , Xu, S. and Jin, Z.
A Simple Protocol for Isolating Mouse Lung Endothelial Cells. ,
Sci Rep
 9 , 1458 , 2019
Cells
Lung endothelial
Enzymes
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.