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Tissue References Search Tool

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Endothelial
Human

Human

Klauzen, P. , Semenova, D. , Kostina, D. , Uspenskiy, V. and Malashicheva, A.
Purinergic Signaling in Pathologic Osteogenic Differentiation of Aortic Valve Interstitial Cells from Patients with Aortic Valve Calcification. , Biomedicines Vol. 11 , 2023
Cells
Valve interstitial and endothelial
HUVEC
Osteoblast
Enzymes
Medium
ECM
Endothelial
Human
Human
Ganguly, A. , Zhang, H. , Sharma, R. , Parsons, S. and Patel, K.
Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions. ,
J Vis Exp
 66 , e4032 , 2012
Cells
Vascular endothelial
Enzymes
Medium
HBSS
Endothelial
Human
Human, 18-68 yr
Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G.
A novel method of isolation, preservation, and expansion of human corneal endothelial cells ,
Inv Ophthal Visual Sci
 48 , 614-20 , 2007
Cells
Corneal endothelial
Enzymes
Medium
DMEM/F12
Endothelial
Human
Human
Moss, S. , Bates, M. , Parrino, P. and Woods, TC.
Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue. ,
Ochsner J
 7 , 133 , 2007
Cells
Endothelial and vascular smooth muscle
Enzymes
Medium
HBSS
Endothelial
Human
Human
Davis, J. , Crampton, S. and Hughes. C.
Isolation of Human Umbilical Vein Endothelial Cells (HUVEC). ,
J Vis Exp
 3 , 183 , 2007
Cells
HUVEC
Enzymes
Medium
HBSS
Endothelial
Human
Human
Silva AP, Kaufmann JE, Vivancos C, Fakan S, Cavadas C, Shaw P, Brunner HR, Vischer U. and Grouzmann E.
Neuropeptide Y expression, localization and cellular transducing effects in HUVEC ,
Biol Cell
 97(6) , 457 , 2005
Cells
HUVEC
Enzymes
Medium
RPMI 1640
Endothelial
Human
Human
Patel, V. , Logan, A. , Watkinson, J. , Uz-Zaman, S. , Sheppard, M. , Ramsden, J. and Eggo, M.
Isolation and Characterization of Human Thyroid Endothelial Cells. ,
Am J Physiol Endocrinol Metab
 284 , E168 , 2003
Cells
Endothelial
Enzymes
Medium
DMEM
Endothelial
Human
Human
Rafiee, P. , Ogawa, H. , Heidemann, J. , Li, M. , Aslam, M. , Lamirand, T. , Fisher, P. , Graewin, S. , Dwinell, M. , Johnson, C. , Shaker, R. and Binion, D.
Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation. ,
Am J Physiol Gastrointest Liver Physiol
 285 , G1277 , 2003
Cells
Esophageal microvascular endothelial
Enzymes
Medium
MCDB-131
Endothelial
Human
Human
Takano Manabu, Meneshian Avedis, Sheikh Emran, Yamakawa Yasuhiko, Wilkins Kirsten Bass, Hopkins Elise A, Bulkley Gregory B.
Rapid upregulation of endothelial P-selectin expression via reactive oxygen species generation ,
Am J Physiol Heart Circ Physiol
 283 , H2054-61 , 2002
Cells
HUVEC
Enzymes
Medium
PBS
Endothelial
Human
Human
Wang D. , Lehman R. , Donner D. , Matli M. , Warren R. and Welton M.
Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells ,
Am J Physiol/Gastro
 282 , G1088 , 2002
Cells
Colonic endothelial cells
Enzymes
Medium
HBSS/5%FBS
Endothelial
Human
Human
Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY. and Koh GY.
Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells ,
Circulation
 101(19) , 2317 , 2000
Cells
HUVEC, porcine pulmonary arterial endothelial cells
Enzymes
Medium
DMEM
Endothelial
Human
Human
Schonbeck U, Sukhova GK, Graber P, Coulter S, Libby P.
Augmented expression of cyclooxygenase-2 in human atherosclerotic lesions ,
Am J Pathol
 155 , 1281-91 , 1999
Cells
Vascular endothelial cells
Enzymes
Collagenase : 0.1%
Medium
DMEM
Endothelial
Human
Human
Sanyal AJ. and Mirshahi F.
A simplified method for the isolation and culture of endothelial cells from pseudointima of transjugular intrahepatic portasystemic shunts ,
Lab Invest
 78(11) , 1469 , 1998
Cells
Hepatic endothelial cells
Enzymes
Collagenase : 0.2%
Medium
DMEM
Endothelial
Human
Human
Grant, D.S. , Lelkes, P.I. , Fukuda, K. and Kleinman, H.K.
Intracellular Mechanisms Involved in Basement Membrane Induced Blood Vessel Differentiation In Vitro ,
In Vitro Cell Dev Biol
 27 , 327 , 1991
Cells
Umbilical vein
HUVEC
Enzymes
Collagenase : 0.1%
Medium
Cord Buffer
(See Reference)
Endothelial
Human
Human
Whitehead, R. and Eeden, P.
A Method For the Prolonged Culture of Colonic Epithelial Cells ,
J Tiss Cul Meth
 13 , 103 , 1991
Cells
Crypt cells
Enzymes
Collagenase : 125 u/ml
Medium
RMPI 1640
Endothelial
Human
Human
Fischer, E. , Stingl, A. and Kirkpatrick, C.
Migration Assay for Endothelial Cells in Multiwells Application to Studies on the Effect of Opioids ,
J Immunol Methods
 128 , 235 , 1990
Cells
Endothelial
Enzymes
Medium
Medium 199
Endothelial
Human
Human
Muller WA, Ratti CM, McDonnell SL, Cohn ZA.
A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions ,
J Exp Med
 170 , 399-414 , 1989
Cells
Human umbilical vein endothelial cells
Enzymes
Collagenase Type 2 : 75 u/ml
Medium
M199
Endothelial
Human
Human
Hoshi, H. , Kan, M. , Chen, J. and McKeehan, W.
Comparative Endocrinology-Paracrinology-Autocrinology of Human Adult Large Vessel Endothelial and Smooth Muscle Cells ,
In Vitro Cell Dev Biol
 24 (4) , 309 , 1988
Cells
Umbilical cord
Smooth muscle
Enzymes
Collagenase : 0.1%
Medium
HEPES
Endothelial
Human
Human
Gerhart, D. Z. , Broderius, M. A. and Drewes, L. R.
Cutlured Human and Canine Endothelial Cells from Brain Microvessels ,
Brain Res Bull
 21 , 785 , 1988
Cells
Microvessels
Enzymes
Medium
DMEM
Endothelial
Human
Human
Hoshi, H. and McKeehan, W.
Isolation, Growth Requirements, Cloning, Prostacylclin Production and Life-Span of Human Adult Endothelial Cells in Low Serum Culture Medium ,
In Vitro Cell Dev Biol
 22 (1) , 51 , 1986
Cells
Endothelial
Enzymes
Collagenase : 0.1%
Medium
HEPES
Endothelial
Human
Human
Sharefkin, J.B. , Fairchild, K.D. , Albus, R.A. , Cruess, D.F. and Rich, N.M.
The Cytoxic Effect of Surgical Glove Powder Particles on Adult Human Vascular Endothelial Cell Cultures: Implications for Clinical Uses of Tissue Culture Techniques ,
J Surg Res
 41 , 463 , 1986
Cells
Endothelial
Saphenous vein
Enzymes
Medium
PBS, CMF
Endothelial
Human
Human
Glassberg, M. , Bern, M. , Coughlin, S. , Haudenschild, C. , Hoyer, L. and Antoniades, H.
Cultured Endothelial Cells Derived from the Human Iliac Arteries ,
In Vitro
 18 , 859 , 1982
Cells
Iliac arteries
Enzymes
Collagenase : 0.25%
Medium
PBS w/Ca++, Mg++, & BSA
(see reference)
Endothelial
Human
Human, adult
Ashida, E. , Johnson, A. and Lipsky, P.
Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro ,
J Clin Invest
 67 , 1490 , 1981
Cells
Peripheral blood mononuclear
Monocytes
T cells
Endothelial
Enzymes
Collagenase : 0.25%
Medium
RPMI 1640
Endothelial
Human
Human (adult and child)
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Endothelial
Human
Human
Gimbrone Jr.
Culture of Vascular Endothelium ,
Prog Hemost Thromb
 3 , 1 , 1976
Cells
Umbilical vein
Enzymes
Collagenase Type 1 : 125 u/ml
Medium
Dulbecco's PBS
Endothelial
Human
Human
Gimbrone, M.A. , Cotran, R.S. and Folkman, J.
Human Vascular Endothelial Cells in Culture: Growth and DNA Synthesis ,
J Cell Biol
 60 , 673 , 1974
Cells
Umbilical cord
Enzymes
Collagenase : 0.1%
Medium
Dulbecco's PBS
Endothelial
Human
Human
Jaffe, E. , Nachman, R. , Becker, C. and Minick, C.
Culture of Human Endothelial Cells Derived from Umbilical Veins. Identification by Morphologic and Immunologic Criteria ,
J Clin Invest
 52 , 2745 , 1973
Cells
Umbilical cord
Enzymes
Collagenase : 0.2%
Medium
Cord buffer
(See Reference)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.