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Liver
Rat

Rat, 8-14 week

Ge, J. , Zheng, Y. , Tsuchida, T. , Furuya, K. , Isoda, H. , Taniguchi, H. , Ohkohchi, N. and Oda, T.
Hepatic Stellate Cells Contribute to Liver Regeneration through Galectins in Hepatic Stem Cell Nliche. ,
Stem Cell Res Ther
 11 , 425 , 2020
Cells
Parenchymal and non-parenchymal
Enzymes
Collagenase : 0.05%
Medium
Williams E
Liver
Rat

Wistar, male, 200-250 g

Berardo, C. , Ferrigno, A. , Siciliano, V. , Richelmi, P. , Vairetti, M. and Pasqua, L.
Isolation of Rat Hepatocytes for Pharmacological Studies on Metabotropic Glutamate Receptor (mGluR) Subtype 5: A Comparison Between Collagenase I Versus Collagenase IV. ,
Eur J Histochem
 64 , 2020
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Rat
Rat, SD, 500-700g
Perepelyuk, M. , Terajima, M. , Wang, A. , Georges, P. , Janmey, P. , Yamauchi, M. and Wells, R.
Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury. ,
Am J Physiol Gastrointest Liver Physiol
 304 , G605 , 2013
Cells
Hepatocytes and sinusoidal endothelial
Enzymes
Medium
HBSS
Liver
Rat
Rat, adult
Shen, L. , Hillebrand, A. , Wang, D. and Liu, M.
Isolation and Primary Culture of Rat Hepatic Cells. ,
J Vis Exp
 64 , e3917 , 2012
Cells
Hepatocytes
Enzymes
Medium
William's
Liver
Rat
Rat, adult
Wen, J. , Olsen, A. , Perepelyuk, M. and Wells, R.
Isolation of Rat Portal Fibroblasts by In Situ Liver Perfusion. ,
J Vis Exp
 64 , e3669 , 2012
Cells
Portal fibroblasts
Enzymes
Medium
DMEM/F-12
Liver
Rat
Rat
Budick-Harmelin, N. , Anavi, S. , Madar, Z. and Tirosh, O.
Fatty Acids-Stress Attenuates Gluconeogenesis Induction and Glucose Production in Primary Hepatocytes. ,
Lipids Health Dis
 11 , 66 , 2012
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Rat
Rat
Gopalakrishnan, S. and Harris, E.
In Vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion. ,
J Vis Exp
 57 , e3138 , 2011
Cells
Hepatocytes and SEC's
Enzymes
Collagenase Type 1 : 0.1-0.2%
Medium
RPMI
Liver
Rat
Rat, SD
Handy, J. , Saxena, N. , Fu, P. , Lin, S. , Mells, J. , Gupta, N. and Anania, F.
Adiponectin Activation of AMPK Disrupts Leptin-Mediated Hepatic Fibrosis via Suppressors of Cytokine Signaling (SOCS-3). ,
J Cell Biochem
 110 , 1195 , 2010
Cells
Hepatic stellate cells
Enzymes
Pronase : 0.02%
Collagenase : see reference
Medium
Medium 199
Liver
Rat
Rat, Wistar, female
Zvibel, I. , Atias, D. , Phillips, A. , Halpern, Z. and Oren, R.
Thyroid Hormones Induce Activation of Rat Hepatic Stellate Cells Through Increased Expression of p75 Neurotrophin Receptor and Direct Activation of Rho. ,
Lab Invest
 90 , 674 , 2010
Cells
Hepatic stellate cells
Enzymes
Collagenase Type 1 : 0.025-0.1%
Pronase : 0.025-0.13%
Medium
DMEM
Liver
Rat
Rat, SD
Chung, C. , Shugrue, C. , Nagar, A. , Doll, J. , Cornwell, M. , Gattu, A. , Kolodecik, T. , Pandol, S. and Gorelick, F.
Ethanol Exposure Depletes Hepatic Pigment Epithelium-Derived Factor, a Novel Lipid Regulator. ,
Gastroenterol
 136 , 331 , 2009
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HBSS
Liver
Rat
Rat, Lewis, male
Bettinger, C. , Kulig, K. , Vacanti, J. , Langer, R. and Borenstein, J.
Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture. ,
Tissue Eng Part A
 15 , 1321 , 2009
Cells
Hepatocytes
Enzymes
Medium
DPBS
Liver
Rat
Rat, 200-300 g
Mula, N. , Cubero, F. , Codesal, J. , de Andres, S. , Escudero, C. , Garcia-Barrutia, S. , Millan, I. , Arahuetes, R. and Maganto, P.
Survival of Allogeneic Hepatocytes Transplanted into the Thymus. ,
Cells Tissues Organs
 188 , 270 , 2008
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Rat
Rat, SD, 500-750 g
Li, Z. , Dranoff, J. , Chan, E. , Uemura, M. and Sevigny, J.and Wells, R.
Transforming Growth Factor-beta and Substrate Stiffness Regulate Portal Fibroblast Activation in Culture. ,
Hepatol
 46 , 1246-56 , 2007
Cells
Portal fibroblasts
Enzymes
Collagenase : 0.03%
Pronase : 0.033%
Hyaluronidase : 0.036%
Medium
DMEM/F-12
Liver
Rat
Rat, SD, 180-200g
Charbonneau, A. , Unson, C. and Lavoie, J.
High-Fat Diet-Induced Hepatic Steatosis Reduces Glucagon Receptor Content in Rat Hepatocytes: Potential Interaction with Acute Exercise. ,
J Physiol
 579 , 255 , 2007
Cells
Hepatocytes
Enzymes
Medium
see reference
Liver
Rat
Rat, SD, male, 230-250g
Doleh, L. and Romani, A.
Biphasic Effect of Extra-Reticular Mg2+ on Hepatic G6P Transport and Hydrolysis. ,
Arch Biochem Biophys
 467 , 283 , 2007
Cells
Hepatocytes
Enzymes
Medium
HEPES
Liver
Rat
Rat, Lewis, 150-200g
Smith, M. and Mooney, D.
Hypoxia Leads to Necrotic Hepatocyte Death. ,
J Biomed Mater Res A
 80 , 520 , 2007
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Rat
Rat, Wistar, 270-330g
Li, W. , Ralphs, K. , Slack, J. and Tosh, D.
Keratinocyte Serum-Free Medium Maintains Long-Term Liver Gene Expression and Function in Cultured Rat Hepatocytes by Preventing the Loss of Liver-Enriched Transcription Factors. ,
Int J Biochem Cell Biol
 39 , 541-54 , 2007
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Rat
Rat, Fisher, E14
Isabel Zvibel, Miri Bronstein, Einav Hubel, Ella Bar-Lev, Zamir Halpern, Ran Oren.
Isolation, characterization and culture of Thy1-positive cells from fetal rat livers ,
World J Gastroenterol
 12 , 3841-7 , 2006
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Rat
Rat, Lewis, female, 150-200 g
Sosef MN, Baust JM, Sugimachi K, Fowler A, Tompkins RG. and Toner M.
Cryopreservation of isolated primary rat hepatocytes: enhanced survival and long-term hepatospecific function ,
Ann Surg
 241 , 125 , 2005
Cells
Hepatocytes
Enzymes
Collagenase : see references
Medium
DMEM
Liver
Rat
Rat
Benten Daniel, Follenzi Antonia, Bhargava Kuldeep K, Kumaran Vinay, Palestro Christopher J, Gupta Sanjeev.
Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice ,
Hepatol
 42 , 140-8 , 2005
Cells
Liver sinusodial endothelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Liver
Rat
Rat, Wistar, 200-300 g
Putz G, Schmider W, Nitschke R, Kurz G, Blum HE.
Synthesis of phospholipid-conjugated bile salts and interaction of bile salt-coated liposomes with cultured hepatocytes ,
J Lipid Res
 46 , 2325-38 , 2005
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Rat
Rat, SD, male, 200-250 g
Raman Priya, Donkin Shawn S, Spurlock Michael E.
Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures ,
Am J Physiol Regul Integr Comp Physiol
 286 , R206-16 , 2004
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
DMEM
Liver
Rat
Rat, adult
Jensen C. , Jauho E. , Santoni-Rugiu E. , Holmskov U. , Teisner B. , Tygstrup N. and Bisgaard H.
Transit-amplifying Ductular (oval) Cells and Their Hepatocytic Progeny are Characterized by a Novel and Distinctive Expression of Delta-like Protein/preadipocyte Factor 1/fetal Antigen 1 ,
Am J Pathol
 164(4) , 1347 , 2004
Cells
Hepatocytes
Enzymes
Pronase : 1%
Medium
Williams E
Liver
Rat
Rat, Wistar
Wang Y. , Liu H. , Guo H. , Wen H. and Liu J.
Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich ,
World J Gastroenterol
 10 , 699 , 2004
Cells
Hepatocytes
Enzymes
Medium
RPMI 1640
Liver
Rat
Rat, Wistar, 220-270g
Annaert, P. , Turncliff, R. , Booth, C. , Thakker, D. and Brouwer, K.
P-Glycoprotein-Mediated In Vitro Biliary Excretion in Sandwich-Cultured Rat Hepatocytes. ,
Drug Metab Dispos
 29 , 1277-83 , 2001
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 200 u/ml
Medium
DMEM
Liver
Rat
Rat, SD, male
Burczynski, M. , McMillian, M. , Parker, J. , Bryant, S. , Leone, A. , Grant, E. , Thorne, J. , Zhong, Z. , Zivin, R. and Johnson, M.
Cytochrome P450 Induction in Rat Hepatocytes Assessed by Quantitative Real-Time Reverse-Transcription Polymerase Chain Reaction and the RNA Invasive Cleavage Assay. ,
Drug Metab Dispos
 29 , 1243 , 2001
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Rat
Rat, SD, male, 230-250 g
Kuddus, R. , Nalesnik, M. , Subbotin, V. , Rao, A. and Gandhi, C.
Enhanced Sythesis and Reduced Metabolism of Endothelin-1 (ET-1) by Hepatocytes - An Important Mechanism of Increased Endogenous Levels of ET-1 in Liver Cirrhosis ,
J Hepatol
 33 , 725 , 2000
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Rat
Rat, male, 3 week
Low-Baselli A. , Hufnagl K. , Parzefall W. , Schulte-Hermann R. and Grasl-Kraupp B.
Initiated Rat Hepatocytes in Primary Culture: A Novel Tool to Study Alterations in Growth Control During the First Stage of Carcinogenesis ,
Carcinogenesis
 21 , 79 , 2000
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Rat
Rat, Wistar, male
Wolz E, Liechti H, Notter B, Oesterhelt G, Kistler A.
Characterization of metabolites of astaxanthin in primary cultures of rat hepatocytes ,
Drug Metab Dispos
 27 , 456-62 , 1999
Cells
Hepatocytes
Enzymes
Collagenase Type 2 : 120 u/ml
Medium
HBSS
Liver
Rat
Rat, Lewis, adult
Matsuura T, Gad MZ, Harrison EH. and Ross AC
Lecithin:retinol acyltransferase and retinyl ester hydrolase activities are differentially regulated by retinoids and have distinct distributions between hepatocyte and nonparenchymal cell fractions of rat liver ,
J Nutr
 127 , 218 , 1997
Cells
Hepatocytes
Enzymes
Collagenase : 60 u/ml
Medium
HEPES
Liver
Rat
Rat
Alston-Smith, J. and Pertoft, H
Isolation of Liver Cells: a System for Obtaining Pure Cells in Monolayer Cultures from a Single Rat Liver , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:14.1 , 1995
Cells
Hepatocytes
Enzymes
Collagenase : 0.04% - 0.06%
Medium
PBS
Liver
Rat
Rat
Matsumoto, K. and Nakamura, T
Techniques for the Isolation and Cultivation of Hepatocytes using Collagenase , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:16.1 , 1995
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
DMEM
Liver
Rat
Rat, SD, male, 200 g
Rana, B. , Mischoulon, D. , Xie, Y. , Bucher, N. and Farmer, S.
Cell-Extracellular Matrix Interactions Can Regulate the Switch between Growth and Differentiation in Rat Hepatocytes: Reciprocal Expression of C/EBPa and Immediate-Early Growth Response Transcription Factors ,
Mol Cell Biol
 14 (9) , 5858 , 1994
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
Medium 199
Liver
Rat
Rat, SD, male, 250-350 g
Dixit, V. , Arthur, M. and Gitnick, G.
Repeated Transplantation of Microencapsulated Hepatocytes for Sustained Correction of Hyperbilirubinemia in Gunn Rats ,
Cell Transplant
 1 , 275 , 1992
Cells
Hepatocytes
Enzymes
Collagenase Type 4 : 200 u/ml
Medium
RPMI 1640
Liver
Rat
Rat, Wistar, adult, male, 100-125 g
Liu, J. , McKim, J. , Liu, Y. and Klaassen, C.
Effects of Butyrate Homologes on Metallothionein Induction in Rat Primary Hepatocyte Cultures ,
In Vitro Cell Dev Biol
 28 , 320 , 1992
Cells
Hepatocytes
Enzymes
Medium
Serum-free medium
Liver
Rat
Rat, Fisher
Blouin, M. and Marceau, N.
Primary Culture of Fetal Rat Liver Bipotential Progenitor Cells ,
J Tiss Cul Meth
 13 , 117 , 1991
Cells
Epithelial
Enzymes
Collagenase : 0.05%
Hyaluronidase : 0.075%
Medium
Eagles's MEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.