For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Intestine
Rat
Rat, SD, embryonic
Bixby, S. , Kruger, G. , Mosher, J. , Joseph, N. and Morrison, S.
Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity. ,
Neuron
 35 , 643 , 2002
Cells
Sciatic nerve and gut neural crest stem
Enzymes
Trypsin : 0.005%
Medium
HBSS
Intestine
Rat
Rat, male, 18-24 day
Quaroni A, Wands J, Trelstad RL, Isselbacher KJ.
Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria ,
J Cell Biol
 80 , 248-65 , 1979
Cells
Intestinal epithelial
Enzymes
Medium
DMEM
Kidney
Rat
Rat, SD, fetal
Gupta, A. , Jadhav, S. , Tripathy, N. and Nityanand, S.
Fetal Kidney Cells Can Ameliorate Ischemic Acute Renal Failure in Rats through Their Anti-Inflammatory, Anti-Apoptotic and Anti-Oxidative Effects. ,
PLoS ONE
 10 , e0131057 , 2015
Cells
Fetal kidney
Enzymes
Medium
MEM
Kidney
Rat
Rat, Lewis, male
Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J.
Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. ,
Lipids Health Dis
 10 , 171 , 2011
Cells
Renal adipose derived cells
Enzymes
Medium
DMEM
Kidney
Rat
Rat
Panico, C. , Luo, Z. , Damiano, S. , Artigiano, F. , Gill, P. and Welch, W.
Renal Proximal Tubular Reabsorption is Reduced in Adult Spontaneously Hypertensive Rats: Roles of Superoxide and Na+/H+ Exchanger 3. ,
Hypertension
 54 , 1291 , 2009
Cells
Proximal tubule
Enzymes
Medium
see reference
Kidney
Rat
Rat, SD, male, 200-250g
Silva, G. , Beierwaltes, W. and Garvin, J.
Extracellular ATP stimulates NO production in rat thick ascending limb ,
Hypertension
 47 , 563-7 , 2006
Cells
Medullary thick ascending limb
Enzymes
Collagenase : 0.1%
Medium
HEPES-saline
Kidney
Rat
Rat, Wistar, 300g
Deng Aihua, Miracle Cynthia M, Lortie Mark, Satriano Joseph, Gabbai Francis B, Munger Karen A, Thomson Scott C, Blantz Roland C.
Kidney oxygen consumption, carbonic anhydrase, and proton secretion ,
Am J Physiol/Renal
 290 , F1009-15 , 2006
Cells
Renal proximal tubules
Enzymes
Medium
DMEM/F12
Kidney
Rat
Rat, Wistar, male, 300-350 g
Deng, A. , Miracle, C. , Suarez, J. , Lortie, M. , Satriano, J. , Thomson, S. , Munger, K. and Blantz, R.
Oxygen Consumption in the Kidney: Effects of Nitric Oxide Synthase Isoforms and Angiotensin II. ,
Kidney Int
 68 , 723 , 2005
Cells
Renal proximal tubules
Enzymes
Medium
DMEM/F-12
Kidney
Rat
Rat
Arystarkhova, E. , Wetzel R. and Sweadner K.
Distribution and Oligomeric Association of Splice Forms of Na(+)-K(+)-ATPase Regulatory Gamma-subunit in Rat Kidney ,
Am J Physiol/Renal
 282(3) , F393 , 2002
Cells
Cortical tubule cells
Enzymes
Collagenase : 0.6%
Medium
DMEM
Kidney
Rat
Rat, SD, male 275-300 g
Amiri Farhad, Shaw Sean, Wang Xiaodan, Tang Jie, Waller Jennifer L, Eaton Douglas C, Marrero Mario B.
Angiotensin II activation of the JAK/STAT pathway in mesangial cells is altered by high glucose ,
Kidney Int Suppl
 61 , 1605-16 , 2002
Cells
Glomerular mesangial cells
Enzymes
Collagenase Type 1 : 250 u/ml
Medium
DMEM
Kidney
Rat
Rat, SD, 1 week
Valencia L. , Bidet M. , Martial S. , Sanchez E. , Melendez E. , Tauc M. , Poujeol C. , Martin D. , Namorado M. , Reyes J. and Poujeol P.
Nifedipine-activated Ca(2+) Permeability in Newborn Rat Cortical Collecting Duct Cells in Primary Culture ,
Am J Physiol Cell Physiol
 280(5) , C1193 , 2001
Cells
Cortical collection duct
Enzymes
Medium
DMEM/Ham's F12
Kidney
Rat
Rat, SD, 250-300 g
Li, N. , Teggatz, E. , Li, P. , Allaire, R. and Zou, A.
Formation and Actions of Cyclic ADP-Ribose in Renal Microvessels ,
Microvasc Res
 60 , 149 , 2000
Cells
Microvessels
Enzymes
Medium
PSS
Kidney
Rat
Rat, SD, 250-300 g
Mattson D. and Wu F.
Nitric Oxide Synthase Activity and Isoforms in Rat Renal Vasculature ,
Hypertension
 35 , 337 , 2000
Cells
Renal
Enzymes
Medium
see reference
Kidney
Rat
Rat, SD, male, 5-6 week
Miyata, N. , Park, F. , Li, X. and Cowley, A.
Distribution of Angiotensin AT1 and AT2 Receptor Subtypes in the Rat Kidney ,
Am J Physiol
 277 , F437 , 1999
Cells
Renal tubules
Enzymes
Collagenase : 0.1%
Medium
see reference
Kidney
Rat
Rat, SD, 225-250 g
Amiri F, Garcia R.
Regulation of angiotensin II receptors and PKC isoforms by glucose in rat mesangial cells ,
Am J Physiol
 276 , F691-9 , 1999
Cells
Glomerular mesangial cells
Enzymes
Collagenase Type 1 : 250 u/ml
Medium
DMEM
Kidney
Rat
Rat, SD, male
Eitle E, Hiranyachattada S, Wang H, Harris PJ.
Inhibition of proximal tubular fluid absorption by nitric oxide and atrial natriuretic peptide in rat kidney ,
Am J Physiol
 274 , C1075-80 , 1998
Cells
Proximal tubule suspensions
Enzymes
Pronase E : 2 u/ml
Medium
HEPES buffer
Kidney
Rat
Rat, SD, male
Schafer, J. , Watkins, M. , Li, L. , Herter, P. , Haxelmans, S. and Schlatter, E.
A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments ,
Am J Physiol
 273 , F650 , 1997
Cells
Nephron segments
Enzymes
Medium
Eagle's MEM
Kidney
Rat
Rat, 12-weeks-old
Ishikawa, S. , Kusaka, I. , Higashiyama, M. , Nagasaka, S. , Saito, T. , Honda, K. and Saito, T.
Cellular Signaling and Proliferative Action of AVP in Mesangium of SHR: Effect of Low Density Lipoprotein ,
Nat Neurosci
 50 , 1506 , 1996
Cells
Renal
Enzymes
Collagenase : 0.1%
Medium
DMEM
Kidney
Rat
Rat, adult
Brion, L. , Schwartz, J. , Lachman, H. , Zavilowitz, B. and Schwartz, G.
Development of H+ Secretion by Cultured Renal Inner Medullary Collecting Duct Cells ,
Am J Physiol
 257 , F486 , 1989
Cells
Inner medullary collecting duct
Papillae
Enzymes
Medium
PBS
Kidney
Rat
Rat, Wistar, female, 100-150 g
Wang, J. , Kester, M. and Dunn, M.
The Effects of Endotoxin on Platelet-Activating Factor Synthesis in Cultured Rat Glomerular Mesangila Cells ,
Biochim Biophys Acta
 969 , 217 , 1988
Cells
Glomerular mesangial
Enzymes
Collagenase : 0.025%
Medium
RPMI 1640
Kidney
Rat
Rat, Wistar, male, 150 - 200 g
Barlet-Bas, C. , Khadouri, C. , Marsey, S. and Doucet, A.
Sodium-Independent In Vitro Induction of Na+,K+-ATPase by Aldosterone in Renal Target Cells: Permissive Effect of Triiodothyronine ,
Proc Natl Acad Sci U S A
 85 , 1707 , 1988
Cells
Renal target
Enzymes
Collagenase : 1.0% (also 0.7%)
Medium
Eagle's MEM
Kidney
Rat
Rat, Wistar, male, adult
Gesek, F. , Wolff, D. and Strandhov, J.
Improved Separation Method for Rat Proximal and Distal Renal Tubules ,
Am J Physiol
 253 , F358 , 1987
Cells
Proximal tubules
Enzymes
Collagenase : 0.2%
Medium
Krebs-Henseleit buffer
Kidney
Rat
Rat,Wistar, 300-400 gm
Vinay, P. , Gougoux, A. and Lemieux, G.
Isolation of a Pure Suspension of Rat Proximal Tubules ,
Am J Physiol
 241 , F403 , 1981
Cells
Proximal tubules
Enzymes
Collagenase : 0.15 %
Medium
Krebs Henseleit solution
Liver
Rat

Rat, 8-14 week

Ge, J. , Zheng, Y. , Tsuchida, T. , Furuya, K. , Isoda, H. , Taniguchi, H. , Ohkohchi, N. and Oda, T.
Hepatic Stellate Cells Contribute to Liver Regeneration through Galectins in Hepatic Stem Cell Nliche. ,
Stem Cell Res Ther
 11 , 425 , 2020
Cells
Parenchymal and non-parenchymal
Enzymes
Collagenase : 0.05%
Medium
Williams E
Liver
Rat

Wistar, male, 200-250 g

Berardo, C. , Ferrigno, A. , Siciliano, V. , Richelmi, P. , Vairetti, M. and Pasqua, L.
Isolation of Rat Hepatocytes for Pharmacological Studies on Metabotropic Glutamate Receptor (mGluR) Subtype 5: A Comparison Between Collagenase I Versus Collagenase IV. ,
Eur J Histochem
 64 , 2020
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Rat
Rat, SD, 500-700g
Perepelyuk, M. , Terajima, M. , Wang, A. , Georges, P. , Janmey, P. , Yamauchi, M. and Wells, R.
Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury. ,
Am J Physiol Gastrointest Liver Physiol
 304 , G605 , 2013
Cells
Hepatocytes and sinusoidal endothelial
Enzymes
Medium
HBSS
Liver
Rat
Rat, adult
Shen, L. , Hillebrand, A. , Wang, D. and Liu, M.
Isolation and Primary Culture of Rat Hepatic Cells. ,
J Vis Exp
 64 , e3917 , 2012
Cells
Hepatocytes
Enzymes
Medium
William's
Liver
Rat
Rat, adult
Wen, J. , Olsen, A. , Perepelyuk, M. and Wells, R.
Isolation of Rat Portal Fibroblasts by In Situ Liver Perfusion. ,
J Vis Exp
 64 , e3669 , 2012
Cells
Portal fibroblasts
Enzymes
Medium
DMEM/F-12
Liver
Rat
Rat
Budick-Harmelin, N. , Anavi, S. , Madar, Z. and Tirosh, O.
Fatty Acids-Stress Attenuates Gluconeogenesis Induction and Glucose Production in Primary Hepatocytes. ,
Lipids Health Dis
 11 , 66 , 2012
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Rat
Rat
Gopalakrishnan, S. and Harris, E.
In Vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion. ,
J Vis Exp
 57 , e3138 , 2011
Cells
Hepatocytes and SEC's
Enzymes
Collagenase Type 1 : 0.1-0.2%
Medium
RPMI
Liver
Rat
Rat, SD
Handy, J. , Saxena, N. , Fu, P. , Lin, S. , Mells, J. , Gupta, N. and Anania, F.
Adiponectin Activation of AMPK Disrupts Leptin-Mediated Hepatic Fibrosis via Suppressors of Cytokine Signaling (SOCS-3). ,
J Cell Biochem
 110 , 1195 , 2010
Cells
Hepatic stellate cells
Enzymes
Pronase : 0.02%
Collagenase : see reference
Medium
Medium 199
Liver
Rat
Rat, Wistar, female
Zvibel, I. , Atias, D. , Phillips, A. , Halpern, Z. and Oren, R.
Thyroid Hormones Induce Activation of Rat Hepatic Stellate Cells Through Increased Expression of p75 Neurotrophin Receptor and Direct Activation of Rho. ,
Lab Invest
 90 , 674 , 2010
Cells
Hepatic stellate cells
Enzymes
Collagenase Type 1 : 0.025-0.1%
Pronase : 0.025-0.13%
Medium
DMEM
Liver
Rat
Rat, SD
Chung, C. , Shugrue, C. , Nagar, A. , Doll, J. , Cornwell, M. , Gattu, A. , Kolodecik, T. , Pandol, S. and Gorelick, F.
Ethanol Exposure Depletes Hepatic Pigment Epithelium-Derived Factor, a Novel Lipid Regulator. ,
Gastroenterol
 136 , 331 , 2009
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HBSS
Liver
Rat
Rat, Lewis, male
Bettinger, C. , Kulig, K. , Vacanti, J. , Langer, R. and Borenstein, J.
Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture. ,
Tissue Eng Part A
 15 , 1321 , 2009
Cells
Hepatocytes
Enzymes
Medium
DPBS
Liver
Rat
Rat, 200-300 g
Mula, N. , Cubero, F. , Codesal, J. , de Andres, S. , Escudero, C. , Garcia-Barrutia, S. , Millan, I. , Arahuetes, R. and Maganto, P.
Survival of Allogeneic Hepatocytes Transplanted into the Thymus. ,
Cells Tissues Organs
 188 , 270 , 2008
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Rat
Rat, SD, 500-750 g
Li, Z. , Dranoff, J. , Chan, E. , Uemura, M. and Sevigny, J.and Wells, R.
Transforming Growth Factor-beta and Substrate Stiffness Regulate Portal Fibroblast Activation in Culture. ,
Hepatol
 46 , 1246-56 , 2007
Cells
Portal fibroblasts
Enzymes
Collagenase : 0.03%
Pronase : 0.033%
Hyaluronidase : 0.036%
Medium
DMEM/F-12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.