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Tissue References Search Tool

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Lung
Mouse
Mouse, 18-20 g
Ebeling, C. , Lam, T. , Gordon, J. , Hollenberg, M. and Vliagoftis, H.
Proteinase-Activated Receptor-2 Promotes Allergic Sensitization to an Inhaled Antigen through a TNF-Mediated Pathway. ,
J Immunol
 179 , 2910 , 2007
Cells
Lung
Enzymes
Medium
HBSS
Lung
Mouse
Mouse, 6-16 week
Abonia, J. , Hallgren, J. , Jones, T. , Shi, T. , Xu, Y. , Koni, P. , Flavell, R. , Boyce, J. , Austen, F. and Gurish, M.
Alpha-4 Integrins and VCAM-1, but not MAdCAM-1, are Essential for Recruitment of Mast Cell Progenitors to the Inflamed Lung. ,
Blood
 108 , 1588-94 , 2006
Cells
Mononuclear
Enzymes
Medium
RPMI 1640
Lung
Mouse
Mouse, 8-12 week
Woolard MD, Hodge LM, Jones HP, Schoeb TR. and Simecka JW
The upper and lower respiratory tracts differ in their requirement of IFN-gamma and IL-4 in controlling respiratory mycoplasma infection and disease ,
J Immunol
 172 , 6875 , 2004
Cells
Mononuclear cells
Enzymes
Medium
RPMI 1640 medium
Lung
Mouse
Mouse, 6-10 week
Andonegui G. , Bonder C. , Green F. , Mullaly S. , Zbytnuik L. , Raharjo E. and Kubes P.
Endothelium-derived Toll-like Receptor-4 is the Key Molecule in LPS-induced Neutrophil Sequestration into Lungs ,
J Clin Invest
 111 , 1011 , 2003
Cells
Murine pulmonary endothelial
Enzymes
Medium
HBSS
Lung
Mouse
Mouse
Huaux F, Liu T, McGarry B, Ullenbruch M. and Phan SH.
Dual roles of IL-4 in lung injury and fibrosis ,
J Immunol
 170 , 2083 , 2003
Cells
Pulmonary T lymphocytes
Enzymes
Collagenase Type 3 : 10 mg/lung
Deoxyribonuclease I : 250 ug/lung
Medium
RPMI medium
Lung
Mouse
Mouse, 6-8 week
Vermaelen KY, Carro-Muino I, Lambrecht BN. and Pauwels RA.
Specific migratory dendritic cells rapidly transport antigen from the airways to the thoracic lymph nodes ,
J Exp Med
 193 , 51 , 2001
Cells
Lung and lymph node cells
Enzymes
Medium
RPMI 1640
Lung
Mouse
Mouse
Freedman SD, Katz MH, Parker EM, Laposata M, Urman MY. and Alvarez JG.
A membrane lipid imbalance plays a role in the phenotypic expression of cystic fibrosis in cftr(-/-) mice ,
Proc Natl Acad Sci U S A
 96 , 13995 , 1999
Cells
Lung
Enzymes
Collagenase : 100 u/ml
Deoxyribonuclease I : 200 u/ml
Medium
Krebs-Henseleit Buffer
Lung
Mouse
Mouse, 6-8 wk
Stampfli MR, Wiley RE, Neigh GS, Gajewska BU, Lei XF, Snider DP, Xing Z, Jordana M.
GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice ,
J Clin Invest
 102 , 1704-14 , 1998
Cells
Lung cells
Enzymes
Collagenase Type 3 : 150 u/ml
Medium
HBSS
Lung
Mouse
Mouse, female, 18-20 g
Dong QG, Bernasconi S, Lostaglio S, De Calmanovici RW, Martin-Padura I, Breviario F, Garlanda C, Ramponi S, Mantovani A,and Vecchi A.
A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants ,
Arterioscler Thromb Vasc Biol
 17 , 1599 , 1997
Cells
Murine endothelial cells
Enzymes
Collagenase Type 1 : 1 mg/ml
Medium
DMEM
Lung
Mouse
Mouse, female
Hamilton-Easton A. and Eichelberger M
Virus-specific antigen presentation by different subsets of cells from lung and mediastinal lymph node tissues of influenza virus-infected mice ,
J Virol
 69 , 6359 , 1995
Cells
Antigen presenting cells
Enzymes
Collagenase : 150 u/ml
Medium
RPMI 1640
Lung
Porcine
Swine fetuses (70 day), adult Amer Dutch 250 day
(also human fetuses, 80 day)
Hinz, R. and Syverton, J.
Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase ,
Proc Soc Exp Biol Med
 101 , 19 , 1959
Cells
Lung
Enzymes
Collagenase : 0.01%
Medium
HBSS
Lung
Rat
Rat
Jones, R. , Capen, D. , Cohen, K. , Munn, L. , Jain, R. and Duda, D.
A Protocol for Phenotypic Detection and Characterization of Vascular Cells of Different Origins in a Lung Neovascularization Model in Rodents. ,
Nat Protoc
 3 , 388-97 , 2008
Cells
Lung
Enzymes
Medium
PBS
Lung
Rat
Rat, SD, 125-150 g
Liebler, J. , Borok, Z. , Li, X. , Zhou, B. , Sandoval, A. and Kim, K.and Crandall, E.
Alveolar Epithelial Type I Cells Express Beta 2-Adrenergic Receptors and G-protein Receptor Kinase 2. ,
J Histochem Cytochem
 52 , 759 , 2004
Cells
Alveolar type I & II
Enzymes
Elastase : 2.5-8 u/ml
Medium
DMEM/F12
Lung
Rat
Rat, SD, 300-400 g
King J, Hamil T, Creighton J, Wu S, Bhat P, McDonald F. and Stevens T
Structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes ,
Microvasc Res
 67 , 139 , 2004
Cells
Pulmonary endothelial cells
Enzymes
Collagenase Type 2 : 1000 u/ml
Medium
DMEM/F-12
Lung
Rat
Rat, Lewis, male, 200-250g
Steinmuller C, Franke-Ullmann G, Lohmann-Matthes ML, Emmendorffer A.
Local activation of nonspecific defense against a respiratory model infection by application of interferon-gamma: comparison between rat alveolar and interstitial lung macrophages ,
Am J Respir Cell Mol Biol
 22 , 481-90 , 2000
Cells
Interstitial lung macrophages
Enzymes
Medium
RPMI-1640
Lung
Rat
Rat, male, 200-250g
Bakhramov, A, Evans, M. and Kozlowski, R
Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree ,
Exp Physiol
 83 , 337-47 , 1998
Cells
Pulmonary arterial myocytes
Enzymes
Collagenase : 0.15%
Papain : 0.15%
Elastase : 0.05%
Medium
PBS
Lung
Rat
Rat, adult
Douglas, W. and Kaighn, M.
Clonal Isolation of Differentiated Rat Lung Cells ,
In Vitro
 10 , 230 , 1974
Cells
Lung
Enzymes
Collagenase : 0.1%
Medium
Moscona saline, CMF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.