Tissue References Search Tool

Canine

Dog, mongrel, 20 Kg

Yau, C. , Rao, L. and Silverman, M.

Sugar Uptake Into a Primary Culture of Dog Kidney Proximal Tubular Cells ,
Can J Physiol Pharmacol
63 , 417 , 1985

Cells

Proximal tubular

Enzymes

Deoxyribonuclease I : 0.0125%

Medium

(see reference)

Fish

Winter flounder (Pseudopleur-onectes americanus)
200 - 500 g

Dickman, K. and Renfro, J.

Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport ,
Am J Physiol
251 , 424 , 1986

Cells

Renal tubule

Enzymes

Trypsin : 0.2%

Trypsin : 0.2%

Medium

CMF solution

Guinea-Pig

Guinea-pig, male, 100 g

Phillips, H.

Dissociation of Single Cells from Lung or Kidney Tissue with Elastase ,
In Vitro
8 , 101 , 1972

Cells

Single cells

Enzymes

Trypsin : 0.25%

Medium

CF salt solution

Human

Human

McAteer, J, Kempson, S. and Evan, A

Culture of Human Renal Cortex Epithelial Cells ,
J Tiss Cul Meth
13 , 143 , 1991

Cells

Renal cortex

Enzymes

Trypsin : 0.1%

Medium

Tissue Culture Grade Water

Human

Human

Heieren, M. , van der Woude, F. and Balfour Jr., H.

Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells ,
Proc Natl Acad Sci U S A
85 , 1642 , 1988

Cells

Mesangial

Enzymes

Trypsin : 0.25%

Medium

DMEM/Ham's F-12

Human

Human, 24 newborn and stillborn
(also rabbit)

De Oca, H. and Malinin, T.

Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys ,
J Clin Microbiol
2 , 243 , 1975

Cells

Renal

Enzymes

Trypsin : 0.25%

Medium

See reference

Monkey

Monkey

Melnick, J. and Wallis, C.

Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells ,
Dev Biol
37 , 77 , 1976

Cells

Kidney

Enzymes

Trypsin : 0.01% - 0.00001%

Medium

Eagle's MEM

Mouse

Mouse

Li, Z. , Zimmerman, K. , Cherakara, S. , Chumley, P. , Collawn, J. , Wang, J. , Haycraft, C. , Song, C. , Chacana, T. , Andersen, R. , Croyle, M. , Aloria, E. , Hombal, R. , Thomas, I. , Chweih, H. , Simanyi, K. , George, J. , Parant, J. , Mrug, M. and Yoder, B.

A Kidney Resident Macrophage Subset is a Candidate Biomarker for Renal Cystic Disease in Preclinical Models. , Dis Model Mech Vol. 16 , 2023

Cells

Macrophages

Enzymes

Collagenase Type 1 : 0.1%

Deoxyribonuclease I : 100 u/ml

Medium

RPMI 1640

Mouse

Mouse

Yuan, Y. , Li, L. , Zhu, L. , Liu, F. , Tang, X. , Liao, G. , Liu, J. , Cheng, J. , Chen, Y. and Lu, Y.

Mesenchymal Stem Cells Elicit Macrophages into M2 Phenotype Via Improving Transcription Factor EB-Mediated Autophagy to Alleviate Diabetic Nephropathy. ,
Stem Cells
38 , 639-652 , 2020

Cells

Mactrophages

Enzymes

Collagenase Type 4 : 0.1%

Deoxyribonuclease I : 0.01%

Medium

DMEM

Mouse

Mouse

Adam, M. , Potter, A. and Potter, S.

Psychrophilic Proteases Dramatically Reduce Single-Cell RNA-Seq Artifacts: A Molecular Atlas of Kidney Development. ,
Development
144 , 3625-3632 , 2017

Cells

Kidney

Enzymes

Deoxyribonuclease I : 125 u/ml

Protease, Bacillus Licheniformis : 1%

Medium

DPBS

Mouse

Mouse

Sedrakyan, S. , Da Sacco, S. , Milanesi, A. , Shiri, L. , Petrosyan, A. , Varimezova, R. , Warburton, D. , Lemley, K. , De Filippo, R. and Perin, L.

Injection of Amniotic Fluid Stem Cells Delays Progression of Renal Fibrosis. ,
J Am Soc Nephrol
23 , 661-73 , 2012

Cells

Glomeruli

Enzymes

Collagenase Type 1 : 0.1%

Deoxyribonuclease I : 100 u/ml

Medium

DMEM

Mouse

Mouse, embryonic

Brown, A. , Blank, U. , Adams, D. , Karolak, M. , Fetting, J. , Hill, B. and Oxburgh, L.

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney. ,
J Vis Exp
50 , e2555 , 2011

Cells

Kidney

Enzymes

Collagenase Type 1 : 0.25%

Pancreatin : 1.0%

Deoxyribonuclease I : 1 u/ml

Medium

HBSS

Mouse

Mouse, 3-7 month

Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.

Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
10 , 1569-79 , 1999

Cells

Fibroblasts, mesangial, smooth muscle

Enzymes

Trypsin : 0.25%

Collagenase : see reference

Soybean Trypsin Inhibitor : 0.05%

Medium

DMEM

Mouse

Mouse, naive SJL (H-2S)

Haverty, T. , Kelly, C. , Hines, W. , Amenta, P. , Watanabe, M. , Harper, R. , Kefalides, N. and Neilson, E.

Characterization of a Renal Tubular Epithelial Cell Line Which Secretes the Autologous Target Antigen of Autoimmune Experimental Interstitial Nephritis ,
J Cell Biol
107 , 1359 , 1988

Cells

Proximal tubular epithelial

Enzymes

Deoxyribonuclease I : 15 µg/ml

Medium

RPMI 1640

Rabbit

Rabbit, New Zealand White, female

Schafer, J. , Watkins, M. , Li, L. , Herter, P. , Haxelmans, S. and Schlatter, E.

A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments ,
Am J Physiol
273 , F650 , 1997

Cells

Nephron segments

Enzymes

Collagenase Type 2 : 0.05%

Deoxyribonuclease I : 50 u/ml

Medium

Eagle's MEM

Rabbit

Rabbit, New Zealand white, female, 2-3 Kg

Rodeheaver, D. , Aleo, M. and Schnellmann, R.

Differences in Enzymatic and Mechanical Isolated Rabbit Renal Proximal Tubules: Comparison in Long-Term Incubation ,
In Vitro Cell Dev Biol
26 , 898 , 1990

Cells

Renal proximal tubules

Enzymes

Deoxyribonuclease I : 70 u/ml

Medium

Modified DME-F12

Rabbit

Rabbit, New Zealand, white

Grenier, F. and Smith, W.

Formation of 6-keto-PGF-1aby Collecting Tubule Cells Isolated from Rabbit Renal Papillae ,
Prostaglandins
16 , 759 , 1978

Cells

Collecting tubule

Enzymes

Trypsin : 0.05%

Medium

Kreb's Ringer buffer

Rat

Rat, SD, male

Schafer, J. , Watkins, M. , Li, L. , Herter, P. , Haxelmans, S. and Schlatter, E.

A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments ,
Am J Physiol
273 , F650 , 1997

Cells

Nephron segments

Enzymes

Collagenase Type 2 : 0.05%

Deoxyribonuclease I : 50 u/ml

Medium

Eagle's MEM

Rat

Rat

Wallach, D. , Anderson, W. and Pastan, I.

Activation of Adenylate Cyclase in Cultured Fibroblasts by Trypsin ,
J Biol Chem
253 , 24 , 1978

Cells

Fibroblasts

Kidney

Enzymes

Trypsin : up to 0.25%

Medium

Dulbecco-Vogt MEM

Tissue Tables (references, grouped by tissue type and species)

Thyroid/Parathyroid