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Tissue References Search Tool

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Endothelial
Rat
Rat, Wistar, male, 3 mo
Nagelkenke, J. , Barto, K. and VanBerkel, T.
In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells ,
J Biol Chem
 258 , 12221 , 1983
Cells
Endothelial
Kupffer
Parenchymal
Enzymes
Collagenase : 0.05%
Pronase : 0.25%
Medium
HBSS
Epithelial
Rat
Rat, male
Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.
Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair ,
J Cell Sci
 112 , 243 , 1999
Cells
Alveolar epithelial
Enzymes
Elastase : 40 u/ml
Medium
DMEM
Epithelial
Rat
Rat, SD, male, 150 - 250 g
Dial, E. , Kao, Y. and Lichtenberger, L.
Effects of 16,16-Dimethyl Prostaglandin E2 On Glycoprotein And Lipid Synthesis of Gastric Epithelial Cells Grown in a Primary Culture ,
In Vitro Cell Dev Biol
 27 , 39 , 1991
Cells
Epithelial
Stomach
Enzymes
Pronase : 0.15%
Medium
Medium 199
Epithelial
Rat
Rat, Fisher 344, male, 8 wks old,
250-300 g
Chang, L. , Wu, R. and Nettesheim, P.
Morphological Changes in Rat Tracheal Cells During The Adaptive and Early Growth Phase in Primary Cell Culture ,
J Cell Sci
 74 , 283 , 1985
Cells
Tracheal epithelial
Enzymes
Pronase : 0.5%
Medium
DMEM
Heart
Rat
Rat, adult
Zhang, Y. , Wang, Y. , Yanni, J. , Qureshi, M. , Logantha, S. , Kassab, S. , Boyett, M. , Gardiner, N. , Sun, H. , Howarth, F. and Dobrzynski, H.
Electrical Conduction System Remodeling in Streptozotocin-Induced Diabetes Mellitus Rat Heart. ,
Front Physiol
 10 , 826 , 2019
Cells
Sinoatrial nodal
Enzymes
Collagenase Type 4 : 220 u/ml
Elastase : 1.4 u/ml
Protease : 0.45 u/ml
Medium
Tyrode solution
Heart
Rat
Rat, SD, 8-10 week
Lee, J. , Ha, J. and Leem, C.
A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca(2+) Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat. ,
Korean J Physiol Pharmacol
 19 , 373-82 , 2015
Cells
Ventricular myocytes
Enzymes
Collagenase : 0.08%
Pronase : 0.004%
Medium
Tyrode's Solution
Heart
Rat
Rat, SD, adult female
Puri S, Bansal DD, Uskokovic MR. and MacGregor RR.
Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3) ,
Am J Physiol/Endo
 278(2) , E293 , 2000
Cells
Heart microvascular cells
Enzymes
CLSPA : 250u/ml
Papain : 5 u/ml
Elastase : 0.8 u/ml
Medium
L-15
Heart
Rat
Rat
Zilberman, A. , Dave, V. , Miano, J. , Olson, E. and Periasamy, M.
Evolutionary Conserved Promoter Region Containing CArg-Like Elements is Crucial for Smooth Muscle Myosin Heavy Chain Gene Expression ,
Circ Res
 82 , 566 , 1998
Cells
Thoracic aorta, smooth muscle
Enzymes
Elastase : 0.02%
Medium
(see reference)
Liver
Rat
Rat, SD
Handy, J. , Saxena, N. , Fu, P. , Lin, S. , Mells, J. , Gupta, N. and Anania, F.
Adiponectin Activation of AMPK Disrupts Leptin-Mediated Hepatic Fibrosis via Suppressors of Cytokine Signaling (SOCS-3). ,
J Cell Biochem
 110 , 1195 , 2010
Cells
Hepatic stellate cells
Enzymes
Pronase : 0.02%
Collagenase : see reference
Medium
Medium 199
Liver
Rat
Rat, Wistar, female
Zvibel, I. , Atias, D. , Phillips, A. , Halpern, Z. and Oren, R.
Thyroid Hormones Induce Activation of Rat Hepatic Stellate Cells Through Increased Expression of p75 Neurotrophin Receptor and Direct Activation of Rho. ,
Lab Invest
 90 , 674 , 2010
Cells
Hepatic stellate cells
Enzymes
Collagenase Type 1 : 0.025-0.1%
Pronase : 0.025-0.13%
Medium
DMEM
Liver
Rat
Rat, SD, 500-750 g
Li, Z. , Dranoff, J. , Chan, E. , Uemura, M. and Sevigny, J.and Wells, R.
Transforming Growth Factor-beta and Substrate Stiffness Regulate Portal Fibroblast Activation in Culture. ,
Hepatol
 46 , 1246-56 , 2007
Cells
Portal fibroblasts
Enzymes
Collagenase : 0.03%
Pronase : 0.033%
Hyaluronidase : 0.036%
Medium
DMEM/F-12
Liver
Rat
Rat, adult
Jensen C. , Jauho E. , Santoni-Rugiu E. , Holmskov U. , Teisner B. , Tygstrup N. and Bisgaard H.
Transit-amplifying Ductular (oval) Cells and Their Hepatocytic Progeny are Characterized by a Novel and Distinctive Expression of Delta-like Protein/preadipocyte Factor 1/fetal Antigen 1 ,
Am J Pathol
 164(4) , 1347 , 2004
Cells
Hepatocytes
Enzymes
Pronase : 1%
Medium
Williams E
Liver
Rat
Rat, CD strain, albino, male, 140 -180 g
Braun, L. , Mead, J. , Panzica, M. , Mikumo, R. , Bell, G. and Fausto, N.
Transforming Growth Factor B mRNA Increases During Liver Regeneration:A Possible Paracrine Mechanism Of Growth Regulation ,
Proc Natl Acad Sci U S A
 85 , 1539 , 1988
Cells
Hepatocytes and Nonparenchymal
Enzymes
Pronase : 0.1%
Medium
HEPES buffer with calcium
Liver
Rat
Rat, Wistar, male, 3 month old
Nagelkenke, J. , Barto, K. and VanBerkel, T.
In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells ,
J Biol Chem
 258 , 12221 , 1983
Cells
Endothelial
Kupffer
Parenchymal
Enzymes
Collagenase : 0.05%
Pronase : 0.25%
Medium
HBSS
Lung
Rat
Rat, SD, 250-300g
Chen J. , Chen Z. , Narasaraju T. , Jin N. and Liu L.
Isolation of Highly Pure Alveolar Epithelial Type I and Type II Cells from Rat Lungs ,
Lab Invest
 84 , 727 , 2004
Cells
Alveolar epithelial
Enzymes
Elastase : 3-4.5 u/ml
Medium
RPMI 1640
Lung
Rat
Rat, SD, 125-150 g
Liebler, J. , Borok, Z. , Li, X. , Zhou, B. , Sandoval, A. and Kim, K.and Crandall, E.
Alveolar Epithelial Type I Cells Express Beta 2-Adrenergic Receptors and G-protein Receptor Kinase 2. ,
J Histochem Cytochem
 52 , 759 , 2004
Cells
Alveolar type I & II
Enzymes
Elastase : 2.5-8 u/ml
Medium
DMEM/F12
Lung
Rat
Rat, SD, 200-225 g
Sunil VR, Connor AJ, Guo Y, Laskin JD. and Laskin DL
Activation of type II alveolar epithelial cells during acute endotoxemia ,
Am J Physiol Lung Cell Mol Physiol
 282 , L872 , 2002
Cells
Type II alveolar epithelial cells
Enzymes
Elastase : 4.2 u/ml
Medium
DMEM
Lung
Rat
Rat, SD, male, adult
Kemp, P. , Kim, K. , Borok. and Crandall, E.
Re-evaluating the Na+ Conductance of Adult Rat Alveolar Type II Pneumocytes: Evidence for the Involvement of cGMP-Activated Cation Channels ,
J Physiol
 536 (3) , 693 , 2001
Cells
Alveolar
Enzymes
Elastase : 2.0 - 2.5 u/ml
Medium
DMEM/Ham's F-12
(see reference)
Lung
Rat
Rat, male
Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.
Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair ,
J Cell Sci
 112 , 243 , 1999
Cells
Alveolar epithelial
Enzymes
Elastase : 40 u/ml
Medium
DMEM
Lung
Rat
Rat, male, 200-250g
Bakhramov, A, Evans, M. and Kozlowski, R
Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree ,
Exp Physiol
 83 , 337-47 , 1998
Cells
Pulmonary arterial myocytes
Enzymes
Collagenase : 0.15%
Papain : 0.15%
Elastase : 0.05%
Medium
PBS
Lung
Rat
Rat, SD, male, 125-175 g
Kim, K. , Suh, D. , Lubman, R. , Danto, S. , Borok, Z. and Crandall, E.
Studies on the Mechanisms of Active Ion Fluxes Across Alveolar Epithelial Cell Monolayers ,
J Tiss Cul Meth
 14 , 187 , 1992
Cells
Alveolar epithelial
Enzymes
Elastase : 2 u/ml
Medium
EBSS
Lung
Rat
Rat, Fischer 344, male
Mangum, J. , Everitt, J. , Bonner, J. , Moore, L. and Brody, A.
Co-Culture of Primary Pulmonary Cells to Model Alveolar Injury and Translocation of Proteins ,
In Vitro Cell Dev Biol
 26 , 1135 , 1990
Cells
Alveolar type II pneumocytes
Enzymes
Elastase : 40 u/ml
Medium
HEPES buffer
(see reference)
Lung
Rat
Rat, SD, male, 250-400 g
Ma, J. , LaCagnin, L. , Bowman, L. and Miles, P.
Carbon Tetrachloride Inhibits Synthesis of Pulmonary Surfactant Disaturated Phosphatidylcholines and ATP Production in Alveolar Type II Cells ,
Biochim Biophys Acta
 1003 , 136 , 1989
Cells
Alveolar type II
Enzymes
Elastase : 40 u/ml
Medium
Phosphate-buffered medium
(see reference)
Lung
Rat
Rat, SD, male, 180-200 g
Dobbs, L. , Gonzalez R. and Williams, M.
An Improved Method for Isolating Type ll Cells in High Yield and Purity ,
Am Rev Respir Dis
 134 , 141 , 1986
Cells
Alveolar type II
Enzymes
Elastase : 4.3 u/ml
Medium
HEPES
(see reference)
Lung
Rat
Rat, SD, male, 150-400 g
Goodman, B. , Fleischer, R. and Crandall, E.
Evidence for Active Na+ Transport by Cultured Monolayers of Pulmonary Alveolar Epithelial Cells ,
Am J Physiol
 245 , C78 , 1983
Cells
Alveolar type II
Enzymes
Elastase : 4 u/ml
Medium
Auto-Pow Eagle's modified MEM
Muscle
Rat
Rat, SD
Zeng, Y. , Liu, H. , Kang, K. , Wang, Z. , Hui, G. , Zhang, X. , Zhong, J. , Peng, W. , Ramchandran, R. , Raj, J. and Gou, D.
Hypoxia Inducible Factor-1 Mediates Expression of miR-322: Potential Role in Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells. ,
Sci Rep
 5 , 12098 , 2015
Cells
Pulmonary arterial smooth muscle
Enzymes
Elastase : 0.05%
Medium
Buffer
Muscle
Rat
Rat
Weber, S. , Gratopp, A. , Akanbi, S. , Rheinlaender, C. , Sallmon, H. , Barikbin, P. and Koehne, P.
Isolation and Culture of Fibroblasts, Vascular Smooth Muscle, and Endothelial Cells From the Fetal Rat Ductus Arteriosus ,
Pediatr Res
 70 , 236 , 2011
Cells
Vascular smooth muscle
Enzymes
Medium
DMEM
Muscle
Rat
Rat, SD, 3 month
Stevenson S. , Rollence M. , Marshall-Neff J. and Liau G.
A Genetically Modified Adenoviral Vector Exhibits Enhanced Gene Transfer of Human Smooth Muscle Cells ,
J Vasc Res
 38(5) , 471 , 2001
Cells
Smooth muscle cells
Enzymes
Medium
M-199
Muscle
Rat
Rat, SD, male, 250-350 g
Hrometz, S. , Edelmann, S. , McCune, D. , Olges, J. , Hadley, R. , Perez ,D. and Piascik, M.
Expression of Multiple Alpha1-Adrenoceptors on Vascular Smooth Muscle: Correlation with the Regulation of Contraction ,
J Pharmacol Exp Ther
 290(1) , 452 , 1999
Cells
Vascular smooth muscle
Enzymes
Elastase : 0.0125%
Medium
DMEM
Muscle
Rat
Rat, 1-3 day
Barone, L. , Wolfe, L. , Faris, B. and Franzblau, C.
Elastin mRNA Levels and Insoluble Elastin Accumulation in Neonatal Rat ,
Biochemistry
 27 , 3175 , 1988
Cells
Smooth muscle, aortic
Enzymes
Elastase : 0.0125%
Medium
DMEM
Muscle
Rat
Rat, SHR, WKY. either sex, 12-19 day, 3 month, and retired breeders
Gordon, D. , Mohai, L. and Schwartz, S.
Induction of Polyploidy in Cultures of Neonatal Rat Aortic Smooth Muscle Cells ,
Circ Res
 59 , 633 , 1986
Cells
Endothelial, aortic
Enzymes
Elastase : 0.05%
Medium
Waymouth's culture medium
Muscle
Rat
Rat, SD, 225-250g
Gunther S, Alexander RW, Atkinson WJ. and Gimbrone MA Jr.
Functional angiotensin II receptors in cultured vascular smooth muscle cells ,
J Cell Biol
 92 , 289 , 1982
Cells
Mesenteric artery smooth muscle cells
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.