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Tissue References Search Tool

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Cartilage
Rabbit
Rabbit, New Zealand, white, 4-6 wk & 22-25 wk
Plaas, A. , Sandy, J. and Kimura, J.
Biosynthesis of Cartilage Proteoglycan & Link Protein Articular Chondrocytes ,
J Biol Chem
 263 , 7560 , 1988
Cells
Chondrocytes
Enzymes
Protease XIV : 5 mg/g of tissue
Medium
Ham's F-12
Endothelial
Rabbit
Rabbit, 2-3Kg
Haley, N. , Shio, H. and Fowler, S.
Characterization of lipid-laden aortic cells from cholesterol-fed rabbits. I. Resolution of aortic cell populations by metrizamide density gradient centrifugation ,
Lab Invest
 37 , 287 , 1977
Cells
Endothelial, aortic
Enzymes
Elastase : 0.2%
Medium
Hanks solution
Heart
Rabbit
Rabbit, New Zealand, 3-3.5 kg
Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.
Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
 117 , 2687-2692 , 2020
Cells
Cardiac Myocytes
Enzymes
Trypsin : 0.08%
Protease XIV : 0.005%
Collagenase Type 1 : 0.1-0.2%
Medium
HEPES
Heart
Rabbit
Rabbit
Verheye, S. , Martinet, W. , Kockx, M. , Knaapen, M. , Salu, K. , Timmermans, J. , Ellis, J. , Kilpatrick, D. and DeMayer, G.
Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy ,
J Am Coll Cardiol
 49 , 706 , 2007
Cells
Smooth muscle cells
Enzymes
Collagenase Type 2 : 300 u/ml
Elastase : 5 u/ml
Medium
F10 Ham
Muscle
Rabbit
Rabbit, New Zealand White
Croons Valerie, Martinet Wim, Herman Arnold G, Timmermans Jean-Pierre, De Meyer Guido R Y.
Selective clearance of macrophages in atherosclerotic plaques by the protein synthesis inhibitor cycloheximide ,
J Pharmacol Exp Ther
 320 , 986-93 , 2007
Cells
Aortic smooth muscle
Enzymes
Collagenase Type 2 : 300 u/ml
Elastase : 5 u/ml
Medium
F10 Ham's
Muscle
Rabbit
Rabbit, adult, 1-2 kg
Benham, C. , Bolton, T. , Byrne, N. and Large, W.
Action of Extremely Applied Adenosine Triphosphate O Single Smooth Muscle Cells Dispersed From Rabbit Ear Artery ,
J Physiol
 387 , 473 , 1987
Cells
Smooth muscle
Enzymes
Elastase : 0.17 - 0.25%
Medium
Saline
Muscle
Rabbit
Rabbit, 0.5-1 Kg
(also guinea-pig, frog, dogfish)
Mitra, R. and Morad, M.
A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
 249 , H1056 , 1985
Cells
Myocytes, heart and stomach
Enzymes
Protease XIV : 0.028%
Medium
Solution C
(see reference)
Muscle
Rabbit
Rabbits, New Zealand white albino, 5-6 months
(also human, Rhesus monkey, rabbit)
Chamley, J. , Campbell, G. , McConnell, J. and Groschel-Stewart, U.
Comparison of Vascular Smooth Muscle Cells from Adult Human, Monkey and Rabbit in Primary Culture and in Subculture ,
Cell Tissue Res
 177 , 503 , 1977
Cells
Smooth muscle, saphenous vein
Enzymes
Elastase : 0.05%
Medium
BSS
Muscle
Rabbit
Rabbit, New England, albino
Day, A. , Phil, D. and Newman, H.
Synthesis of Phospholipid by Foam Cells Isolated from Rabbit Atherosclerotic Lesions ,
Circ Res
 XIX , 122 , 1966
Cells
Thoracic aorta
Enzymes
Elastase : 0.008%
Medium
Kreb's Ringer

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.