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Tissue References Search Tool

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Adipose/Fat
Rat
Rat, SD, various weights and ages
Woodward, Julie A. and Saggerson, E.
Effect of Adenosine Deaminase, N6-Phenylisopropyladenosine and Hypothroidism on the Responsiveness of Rat Brown Adipocytes to Noradrenaline ,
Biochem J
 238 , 395 , 1986
Cells
Brown adipocytes
Interscapular & cervical depots
Enzymes
Medium
Krebs Ringer bicarbonate buffer
Colon
Rat
Rat, adult
Somara, S. , Bashllari, D. , Gilmont, R. and Bitar, K.
Real-Time Dynamic Movement of Caveolin-1 During Smooth Muscle Contraction of Human Colon and Aged Rat Colon Transfected with Caveolin-1 cDNA. ,
Am J Physiol Gastrointest Liver Physiol
 300 , G1022 , 2011
Cells
Colon smooth muscle
Enzymes
Medium
DMEM
Endothelial
Rat
Rat, SD, male, 250-300 g
Schwertschlag, U.S. and Whorton, A.R.
Platelet-Activating Factor-Induced Homologous and Heterologous Desensitization In Cultured Vascular Smooth Muscle Cells ,
J Biol Chem
 263 , 13791 , 1988
Cells
Smooth muscle, aorta
Enzymes
Medium
HBSS with 0.2 mM Ca++
Epithelial
Rat
Rat, male
Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.
Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair ,
J Cell Sci
 112 , 243 , 1999
Cells
Alveolar epithelial
Enzymes
Elastase : 40 u/ml
Medium
DMEM
Heart
Rat
Rat, adult
Zhang, Y. , Wang, Y. , Yanni, J. , Qureshi, M. , Logantha, S. , Kassab, S. , Boyett, M. , Gardiner, N. , Sun, H. , Howarth, F. and Dobrzynski, H.
Electrical Conduction System Remodeling in Streptozotocin-Induced Diabetes Mellitus Rat Heart. ,
Front Physiol
 10 , 826 , 2019
Cells
Sinoatrial nodal
Enzymes
Collagenase Type 4 : 220 u/ml
Elastase : 1.4 u/ml
Protease : 0.45 u/ml
Medium
Tyrode solution
Heart
Rat
Rat, SD, adult female
Puri S, Bansal DD, Uskokovic MR. and MacGregor RR.
Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3) ,
Am J Physiol/Endo
 278(2) , E293 , 2000
Cells
Heart microvascular cells
Enzymes
CLSPA : 250u/ml
Papain : 5 u/ml
Elastase : 0.8 u/ml
Medium
L-15
Heart
Rat
Rat
Zilberman, A. , Dave, V. , Miano, J. , Olson, E. and Periasamy, M.
Evolutionary Conserved Promoter Region Containing CArg-Like Elements is Crucial for Smooth Muscle Myosin Heavy Chain Gene Expression ,
Circ Res
 82 , 566 , 1998
Cells
Thoracic aorta, smooth muscle
Enzymes
Elastase : 0.02%
Medium
(see reference)
Lung
Rat
Rat, SD, 250-300g
Chen J. , Chen Z. , Narasaraju T. , Jin N. and Liu L.
Isolation of Highly Pure Alveolar Epithelial Type I and Type II Cells from Rat Lungs ,
Lab Invest
 84 , 727 , 2004
Cells
Alveolar epithelial
Enzymes
Elastase : 3-4.5 u/ml
Medium
RPMI 1640
Lung
Rat
Rat, SD, 125-150 g
Liebler, J. , Borok, Z. , Li, X. , Zhou, B. , Sandoval, A. and Kim, K.and Crandall, E.
Alveolar Epithelial Type I Cells Express Beta 2-Adrenergic Receptors and G-protein Receptor Kinase 2. ,
J Histochem Cytochem
 52 , 759 , 2004
Cells
Alveolar type I & II
Enzymes
Elastase : 2.5-8 u/ml
Medium
DMEM/F12
Lung
Rat
Rat, SD, 200-225 g
Sunil VR, Connor AJ, Guo Y, Laskin JD. and Laskin DL
Activation of type II alveolar epithelial cells during acute endotoxemia ,
Am J Physiol Lung Cell Mol Physiol
 282 , L872 , 2002
Cells
Type II alveolar epithelial cells
Enzymes
Elastase : 4.2 u/ml
Medium
DMEM
Lung
Rat
Rat, SD, male, adult
Kemp, P. , Kim, K. , Borok. and Crandall, E.
Re-evaluating the Na+ Conductance of Adult Rat Alveolar Type II Pneumocytes: Evidence for the Involvement of cGMP-Activated Cation Channels ,
J Physiol
 536 (3) , 693 , 2001
Cells
Alveolar
Enzymes
Elastase : 2.0 - 2.5 u/ml
Medium
DMEM/Ham's F-12
(see reference)
Lung
Rat
Rat, male
Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.
Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair ,
J Cell Sci
 112 , 243 , 1999
Cells
Alveolar epithelial
Enzymes
Elastase : 40 u/ml
Medium
DMEM
Lung
Rat
Rat, male, 200-250g
Bakhramov, A, Evans, M. and Kozlowski, R
Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree ,
Exp Physiol
 83 , 337-47 , 1998
Cells
Pulmonary arterial myocytes
Enzymes
Collagenase : 0.15%
Papain : 0.15%
Elastase : 0.05%
Medium
PBS
Lung
Rat
Rat, SD, male, 125-175 g
Kim, K. , Suh, D. , Lubman, R. , Danto, S. , Borok, Z. and Crandall, E.
Studies on the Mechanisms of Active Ion Fluxes Across Alveolar Epithelial Cell Monolayers ,
J Tiss Cul Meth
 14 , 187 , 1992
Cells
Alveolar epithelial
Enzymes
Elastase : 2 u/ml
Medium
EBSS
Lung
Rat
Rat, Fischer 344, male
Mangum, J. , Everitt, J. , Bonner, J. , Moore, L. and Brody, A.
Co-Culture of Primary Pulmonary Cells to Model Alveolar Injury and Translocation of Proteins ,
In Vitro Cell Dev Biol
 26 , 1135 , 1990
Cells
Alveolar type II pneumocytes
Enzymes
Elastase : 40 u/ml
Medium
HEPES buffer
(see reference)
Lung
Rat
Rat, SD, male, 250-400 g
Ma, J. , LaCagnin, L. , Bowman, L. and Miles, P.
Carbon Tetrachloride Inhibits Synthesis of Pulmonary Surfactant Disaturated Phosphatidylcholines and ATP Production in Alveolar Type II Cells ,
Biochim Biophys Acta
 1003 , 136 , 1989
Cells
Alveolar type II
Enzymes
Elastase : 40 u/ml
Medium
Phosphate-buffered medium
(see reference)
Lung
Rat
Rat, SD, male, 180-200 g
Dobbs, L. , Gonzalez R. and Williams, M.
An Improved Method for Isolating Type ll Cells in High Yield and Purity ,
Am Rev Respir Dis
 134 , 141 , 1986
Cells
Alveolar type II
Enzymes
Elastase : 4.3 u/ml
Medium
HEPES
(see reference)
Lung
Rat
Rat, SD, male, 150-400 g
Goodman, B. , Fleischer, R. and Crandall, E.
Evidence for Active Na+ Transport by Cultured Monolayers of Pulmonary Alveolar Epithelial Cells ,
Am J Physiol
 245 , C78 , 1983
Cells
Alveolar type II
Enzymes
Elastase : 4 u/ml
Medium
Auto-Pow Eagle's modified MEM
Muscle
Rat
Rat, SD
Zeng, Y. , Liu, H. , Kang, K. , Wang, Z. , Hui, G. , Zhang, X. , Zhong, J. , Peng, W. , Ramchandran, R. , Raj, J. and Gou, D.
Hypoxia Inducible Factor-1 Mediates Expression of miR-322: Potential Role in Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells. ,
Sci Rep
 5 , 12098 , 2015
Cells
Pulmonary arterial smooth muscle
Enzymes
Elastase : 0.05%
Medium
Buffer
Muscle
Rat
Rat
Weber, S. , Gratopp, A. , Akanbi, S. , Rheinlaender, C. , Sallmon, H. , Barikbin, P. and Koehne, P.
Isolation and Culture of Fibroblasts, Vascular Smooth Muscle, and Endothelial Cells From the Fetal Rat Ductus Arteriosus ,
Pediatr Res
 70 , 236 , 2011
Cells
Vascular smooth muscle
Enzymes
Medium
DMEM
Muscle
Rat
Rat, SD, 3 month
Stevenson S. , Rollence M. , Marshall-Neff J. and Liau G.
A Genetically Modified Adenoviral Vector Exhibits Enhanced Gene Transfer of Human Smooth Muscle Cells ,
J Vasc Res
 38(5) , 471 , 2001
Cells
Smooth muscle cells
Enzymes
Medium
M-199
Muscle
Rat
Rat, SD, male, 250-350 g
Hrometz, S. , Edelmann, S. , McCune, D. , Olges, J. , Hadley, R. , Perez ,D. and Piascik, M.
Expression of Multiple Alpha1-Adrenoceptors on Vascular Smooth Muscle: Correlation with the Regulation of Contraction ,
J Pharmacol Exp Ther
 290(1) , 452 , 1999
Cells
Vascular smooth muscle
Enzymes
Elastase : 0.0125%
Medium
DMEM
Muscle
Rat
Rat, 1-3 day
Barone, L. , Wolfe, L. , Faris, B. and Franzblau, C.
Elastin mRNA Levels and Insoluble Elastin Accumulation in Neonatal Rat ,
Biochemistry
 27 , 3175 , 1988
Cells
Smooth muscle, aortic
Enzymes
Elastase : 0.0125%
Medium
DMEM
Muscle
Rat
Rat, SHR, WKY. either sex, 12-19 day, 3 month, and retired breeders
Gordon, D. , Mohai, L. and Schwartz, S.
Induction of Polyploidy in Cultures of Neonatal Rat Aortic Smooth Muscle Cells ,
Circ Res
 59 , 633 , 1986
Cells
Endothelial, aortic
Enzymes
Elastase : 0.05%
Medium
Waymouth's culture medium
Muscle
Rat
Rat, SD, 225-250g
Gunther S, Alexander RW, Atkinson WJ. and Gimbrone MA Jr.
Functional angiotensin II receptors in cultured vascular smooth muscle cells ,
J Cell Biol
 92 , 289 , 1982
Cells
Mesenteric artery smooth muscle cells
Enzymes
Medium
HBSS
Neural
Rat
Rat, embryonic 14.5 day
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Pancreas
Rat
Rat, SD, adult
Li, J. , Zhou, R. , Bie, B. , Huang, N. , Guo, Y. , Chen, H. , Shi, M. , Yang, J. , Zhang, J. and Li, Z.
Emodin and Baicalein Inhibit Sodium Taurocholate-Induced Vacuole Formation in Pancreatic Acinar Cells. ,
World J Gastroenterol
 24 , 35-45 , 2018
Cells
Acinar
Enzymes
Medium
Culture media
Pancreas
Rat
Rat, SD, male
Ji B, Kopin AS, Logsdon CD.
Species differences between rat and mouse CCKA receptors determine the divergent acinar cell response to the cholecystokinin analog JMV-180 ,
J Biol Chem
 275 , 19115-20 , 2000
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Rat
Rat
Blinman TA, Gukovsky I, Mouria M, Zaninovic V, Livingston E, Pandol SJ, Gukovskaya AS.
Activation of pancreatic acinar cells on isolation from tissue: cytokine upregulation via p38 MAP kinase ,
Am J Physiol Cell Physiol
 279 , C1993-2003 , 2000
Cells
Pancreatic acini
Enzymes
CLSPA : 30 u/ml
Collagenase Type 4 : 30 u/ml
Medium
M199
Pancreas
Rat
Rat, SD, male, 150 - 200 g
Menozzi, D. , Sato, S. , Jensen, R. and Gardner, J.
Cyclic GMP Does Not Inhibit Protein Kinase C-Mediated Enzyme Secretion in Rat Pancreatic Acini ,
J Biol Chem
 264 , 995 , 1989
Cells
Acinar
Enzymes
Medium
HEPES

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.