Tissue References Search Tool

Lung

Rat

Rat, SD, 250-300g

Chen J. , Chen Z. , Narasaraju T. , Jin N. and Liu L.

Isolation of Highly Pure Alveolar Epithelial Type I and Type II Cells from Rat Lungs ,
Lab Invest
84 , 727 , 2004

Cells

Alveolar epithelial

Enzymes

Elastase : 3-4.5 u/ml

Medium

RPMI 1640

Lung

Rat

Rat, SD, 125-150 g

Liebler, J. , Borok, Z. , Li, X. , Zhou, B. , Sandoval, A. and Kim, K.and Crandall, E.

Alveolar Epithelial Type I Cells Express Beta 2-Adrenergic Receptors and G-protein Receptor Kinase 2. ,
J Histochem Cytochem
52 , 759 , 2004

Cells

Alveolar type I & II

Enzymes

Elastase : 2.5-8 u/ml

Collagenase Type 1 : 1.0%

Medium

DMEM/F12

Lung

Rat

Rat, SD, 200-225 g

Sunil VR, Connor AJ, Guo Y, Laskin JD. and Laskin DL

Activation of type II alveolar epithelial cells during acute endotoxemia ,
Am J Physiol Lung Cell Mol Physiol
282 , L872 , 2002

Cells

Type II alveolar epithelial cells

Enzymes

Elastase : 4.2 u/ml

Deoxyribonuclease I : 0.0001%

Medium

DMEM

Lung

Rat

Rat, SD, male, adult

Kemp, P. , Kim, K. , Borok. and Crandall, E.

Re-evaluating the Na+ Conductance of Adult Rat Alveolar Type II Pneumocytes: Evidence for the Involvement of cGMP-Activated Cation Channels ,
J Physiol
536 (3) , 693 , 2001

Cells

Alveolar

Enzymes

Elastase : 2.0 - 2.5 u/ml

Medium

DMEM/Ham's F-12

(see reference)

Lung

Rat

Rat, male

Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.

Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair ,
J Cell Sci
112 , 243 , 1999

Cells

Alveolar epithelial

Enzymes

Elastase : 40 u/ml

Medium

DMEM

Lung

Rat

Rat, male, 200-250g

Bakhramov, A, Evans, M. and Kozlowski, R

Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree ,
Exp Physiol
83 , 337-47 , 1998

Cells

Pulmonary arterial myocytes

Enzymes

Collagenase : 0.15%

Papain : 0.15%

Elastase : 0.05%

Medium

PBS

Lung

Rat

Rat, SD, male, 125-175 g

Kim, K. , Suh, D. , Lubman, R. , Danto, S. , Borok, Z. and Crandall, E.

Studies on the Mechanisms of Active Ion Fluxes Across Alveolar Epithelial Cell Monolayers ,
J Tiss Cul Meth
14 , 187 , 1992

Cells

Alveolar epithelial

Enzymes

Elastase : 2 u/ml

Medium

EBSS

Lung

Rat

Rat, Fischer 344, male

Mangum, J. , Everitt, J. , Bonner, J. , Moore, L. and Brody, A.

Co-Culture of Primary Pulmonary Cells to Model Alveolar Injury and Translocation of Proteins ,
In Vitro Cell Dev Biol
26 , 1135 , 1990

Cells

Alveolar type II pneumocytes

Enzymes

Elastase : 40 u/ml

Medium

HEPES buffer

(see reference)

Lung

Rat

Rat, SD, male, 250-400 g

Ma, J. , LaCagnin, L. , Bowman, L. and Miles, P.

Carbon Tetrachloride Inhibits Synthesis of Pulmonary Surfactant Disaturated Phosphatidylcholines and ATP Production in Alveolar Type II Cells ,
Biochim Biophys Acta
1003 , 136 , 1989

Cells

Alveolar type II

Enzymes

Elastase : 40 u/ml

Medium

Phosphate-buffered medium

(see reference)

Lung

Rat

Rat, SD, male, 180-200 g

Dobbs, L. , Gonzalez R. and Williams, M.

An Improved Method for Isolating Type ll Cells in High Yield and Purity ,
Am Rev Respir Dis
134 , 141 , 1986

Cells

Alveolar type II

Enzymes

Elastase : 4.3 u/ml

Medium

HEPES

(see reference)

Lung

Rat

Rat, SD, male, 150-400 g

Goodman, B. , Fleischer, R. and Crandall, E.

Evidence for Active Na+ Transport by Cultured Monolayers of Pulmonary Alveolar Epithelial Cells ,
Am J Physiol
245 , C78 , 1983

Cells

Alveolar type II

Enzymes

Elastase : 4 u/ml

Medium

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Tissue References Search Tool

Tissue Tables (references, grouped by tissue type and species)