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Tissue References Search Tool

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Heart
Human
Human
Todor Anastassia, Sharov Victor G, Tanhehco Elaine J, Silverman Norman, Bernabei Alvise, Sabbah Hani N.
Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes ,
Am J Physiol Heart Circ Physiol
 283 , H990-5 , 2002
Cells
Cardiomyocytes
Enzymes
Protease XIV : 0.013%
Medium
HEPES solution
Heart
Human
Human, fetal, 14.5 week gestation
Goldman, B. and Wurzel, J.
Effects of Subcultivation and Culture Medium on Differentiation of Human Fetal Cardiac Myocytes ,
In Vitro Cell Dev Biol
 28 , 109 , 1992
Cells
Myocytes
Enzymes
Hyaluronidase : 0.1%
Medium
HBSS with Calcium
Heart
Mouse
Mouse
Hu, D. , Yin, C. , Mohanta, S. , Weber, C. and Habenicht, A.
Prepartaion of Single Cell Suspensions from Mouse Aorta ,
Bio Protoc
 6 , 11 , 2016
Cells
Aortic
Enzymes
Collagenase Type 1 : 400 u/ml
Collagenase : 120 u/ml
Hyaluronidase : 60 u/ml
Medium
DPBS
Heart
Mouse
Mouse, male, 12-18 week
Jian, Z, Chen, Y, Shimkunas, R, Jian, Y, Jaradeh, M, Chavez, K, Chiamvimonvat, N, Tardiff, J, Izu, L, Ross, R. and Chen-Izu, Y
In Vivo Cannulation Methods for Cardiomyocytes Isolation from Heart Disease Models. ,
PLoS ONE
 11 , e0160605 , 2016
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 300 u/ml
Protease XIV : 0.004%
Medium
Perfusion solution (see reference)
Heart
Mouse

Mouse, 8-12 week

Ackers-Johnson, M. , Li, P. , Holmes, A. , O'Brien, S. , Pavlovic, D. and Foo, R.
A Simplified, Langendorff-Free Method for Concomitant Isolation of Viable Cardiac Myocytes and Nonmyocytes From the Adult Mouse Heart. ,
Circ Res
 119 , 909-20 , 2016
Cells
Cardiomyocytes
Enzymes
Protease XIV : 0.005%
Medium
Perfusion buffer (see reference)
Heart
Mouse
Mouse, adult, 9-13 week
Kabaeva, Z. , Zhao, M. and Michele, D.
Blebbistatin Extends Culture Life of Adult Mouse Cardiac Myocytes and Allows Efficient and Stable Transgene Expression. ,
Am J Physiol Heart Circ Physiol
 294 , H1667-74 , 2008
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 620 u/ml
Protease XIV : 0.104 u/ml
Medium
Myocyte buffer see reference
Heart
Mouse
Mouse, adult, 6-12 week
Shioya, T.
A Simple Technique for Isolating Healthy Heart Cells from Mouse Models. ,
J Physiol Sci
 57 , 327-35 , 2007
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.006%
Protease XIV : 0.006%
Medium
see reference
Heart
Mouse
Mouse, 2-4 mo
Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP.
Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology ,
Am J Physiol Heart Circ Physiol
 279 , H429-36 , 2000
Cells
Ventricular myocytes
Enzymes
Protease XIV : 0.002%
Medium
MEM
Heart
Mouse
Mouse, 6-9 or 21-41 day
Valenzuela, D. , Han, X. , Mende, U. , Fankhauser, C. , Mashimo, H. , Huang, P. , Pfeffer, J. , Neer, E. and Fishman, M.
G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart ,
Proc Natl Acad Sci U S A
 94(5) , 1727 , 1997
Cells
Ventricular and atrial myocytes
Enzymes
Collagenase Type 2 : 95 u/ml
Hyaluronidase : 172.5 u/ml
Trypsin : 0.002%
Medium
DMEM/Tyrodes
Heart
Ovine
Ovine
Voigt, N. , Pearman, C. , Dobrev, D. and Dibb, K.
Methods for Isolating Atrial Cells from Large Mammals and Humans. ,
J Mol Cell Cardiol
 86 , 187-98 , 2015
Cells
Atrial myocytes
Enzymes
Protease XIV : 0.006%
Medium
Tyrode's solution
Heart
Rabbit
Rabbit, New Zealand, 3-3.5 kg
Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.
Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
 117 , 2687-2692 , 2020
Cells
Cardiac Myocytes
Enzymes
Trypsin : 0.08%
Protease XIV : 0.005%
Collagenase Type 1 : 0.1-0.2%
Medium
HEPES
Heart
Rabbit
Rabbit, New Zealand, 2.3-2.9 kg
Farkas, A. , Acsai, K. , Nagy, N. , Toth, A. , Fulop, F. , Seprenyi, G. , Birinyi, P. , Nanasi, P. , Forster, T. , Csanady, M. , Papp, J. , Varrro, A. and Farkas, A.
Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart. ,
Br J Pharmacol
 154 , 93 , 2008
Cells
Cardiomyocytes
Enzymes
Hyaluronidase : see reference
Protease : see reference
Medium
Krebs-Henseleit
Heart
Rabbit
Rabbit, New Zealand, white, male, 2-3 kg
Buxton, I. and Brunton, L.
Compartments of Cyclic AMP and Protein Kinase in Mammalian Cardiomyocytes ,
J Biol Chem
 258 (17) , 10233 , 1983
Cells
Cardiomyocytes
Enzymes
Hyaluronidase : 0.5%
Medium
Eagle's MEM
Heart
Rabbit
Rabbit, adult, 2 kg
Dani, A. , Cittadini, A. , Flamini, G. , Festuccia, G. and Terranova, T.
Preparation and Some Properties of Isolated Beating Myocytes from Adult Rabbit Heart ,
J Mol Cell Cardiol
 9 , 777 , 1977
Cells
Myocytes
Enzymes
Hyaluronidase : 0.007%
Medium
Kreb's Ringer
Heart
Rat
Rat, adult
Xu, X. and Colecraft, H.
Primary Culture of Adult Rat Heart Myocytes. ,
J Vis Exp
 28 , 1308 , 2009
Cells
Myocytes
Enzymes
Protease XIV : 0.02%
Medium
Media 199
Heart
Rat
Rat, SD, 200-250 g
Farkas, A. , Acsai, K. , Nagy, N. , Toth, A. , Fulop, F. , Seprenyi, G. , Birinyi, P. , Nanasi, P. , Forster, T. , Csanady, M. , Papp, J. , Varrro, A. and Farkas, A.
Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart. ,
Br J Pharmacol
 154 , 93 , 2008
Cells
Cardiomyocytes
Enzymes
Hyaluronidase : see reference
Protease : see reference
Medium
Krebs-Henseleit
Heart
Rat
Rat, SD, male
Stagg Mark A, Coppen Steven R, Suzuki Ken, Varela-Carver Anabel, Lee Joon, Brand Nigel J, Fukushima Satsuki, Yacoub Magdi H, Terracciano Cesare M.
Evaluation of frequency, type, and function of gap junctions between skeletal myoblasts overexpressing connexin43 and cardiomyocytes: relevance to cell transplantation ,
FASEB J
 20 , 744-6 , 2006
Cells
Ventricular myocytes
Enzymes
Collagenase : 0.13%
Hyaluronidase : 0.06%
Medium
DMEM
Heart
Rat
Rat, SD, 300-350 g
Niederbichler Andreas D, Hoesel Laszlo M, Westfall Margaret V, Gao Hongwei, Ipaktchi Kyros R, Sun Lei, Zetoune Firas S, Su Grace L, Arbabi Saman, Sarma J Vidya, Wang StewartC, Hemmila MarkR, Ward PeterA.
An essential role for complement C5a in the pathogenesis of septic cardiac dysfunction ,
J Exp Med
 203 , 53-61 , 2006
Cells
Cardiomyocytes
Enzymes
Medium
DMEM
Heart
Rat
Rat, 50 g
Aberle II NS. and Ren J.
Experimental Assessment of the Role of Acetaldehyde in Alcoholic Cardiomyopathy ,
Biol Proced Online
 5 , 1 , 2003
Cells
Ventricular myocytes
Enzymes
Collagenase : 223 u/ml
Hyaluronidase : 0.01%
Trypsin : 0.002%
Medium
Medium 199
Heart
Rat
Rat, adult, 220-280g
Gordon Jennifer M, Dusting Gregory J, Woodman Owen L, Ritchie Rebecca H.
Cardioprotective action of CRF peptide urocortin against simulated ischemia in adult rat cardiomyocytes ,
Am J Physiol Heart Circ Physiol
 284 , H330-6 , 2003
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 178 u/ml
Hyaluronidase : 0.01%
Medium
Krebs-Henseleit, CF
Heart
Rat
Rat, SD, pregnant female
DeAlmeida, A. , Mustin, D. , Forman, M. , Brower, G. , Janicki, J. and Carver, W.
Effects of mast cells on the behavior of isolated heart fibroblasts: modulation of collagen remodeling and gene expression ,
J Cell Physiol
 191 , 51 , 2002
Cells
Peritoneal mast
Enzymes
Hyaluronidase : 100 u/ml
Medium
DMEM (see reference)
Heart
Rat
Rat, adult
Ren J. and Wold LE.
Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging ,
Biol Proced Online
 3 , 43 , 2001
Cells
Ventricular myocytes
Enzymes
Collagenase : 223 u/ml
Hyaluronidase : 0.01%
Trypsin : 0.002%
Medium
Tyrode solution
Heart
Rat
Rat, SD, female, 200g
Westfall M V, Rust E M, Metzger J M.
Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function ,
Proc Natl Acad Sci U S A
 94 , 5444-9 , 1997
Cells
Ventricular cardiac myocytes
Enzymes
Medium
Krebs-Henseleit buffer
Heart
Rat
Rat, SD, male, 250-350 g
Kim, D. and Smith, T.W.
Temporal Variation in Contractile State And [Ca++] in Isolated Adult Rat And Guinea Pig Cardiac Myocytes , Biology of Isolated Adult Cardiac Myocytes , (Clark, Decker, Borg, eds) , Elsevier Science Publishing Co. Inc. , 370 , 1988
Cells
Myocytes, heart
Enzymes
Hyaluronidase : 0.03%
Medium
Bicarbonate-buffered medium
Heart
Rat
Rat, male/female, 200-250 g
Nag, A. and Cheng, M.
Adult Mammalian Cardiac Muscle Cells in Culture ,
Tissue Cell
 13 , 515 , 1981
Cells
Muscle
Enzymes
Hyaluronidase : 0.1%
Medium
Krebs Ringer phosphate buffer, CMF
Heart
Rat
Rat, SD, adult, male, 200-250 g
Frangakis, C. , Bahl, J. , McDaniel, H. and Bressler, R.
Tolerance to Physiological Calcium by Isolated Mycocytes from the Adult Rat Heart; An Improved Cellular Preparation ,
Life Sci
 27 , 815 , 1980
Cells
Myocytes
Enzymes
Hyaluronidase : 0.10%
Medium
Joklik MEM, CF
Heart
Rat
Rat, male, 200-300 g
Farmer, B. , Harris, R. , Jolly, W. , Hathaway, D. , Katzberg, A. , Watanabe, A. , Whitlow, A. and Besch, H.
Isolation and Characterization of Adult Rat Heart Cells ,
Arch Biochem Biophys
 179 , 545 , 1977
Cells
Myocytes
Enzymes
Hyaluronidase : 82 u/ml
Medium
Kreb's Henseleit buffer
Heart
Rat
Rat, female, albino, 200-250 g
Nag, A. , Fischman, D. , Aumont, M. and Zak, R.
Studies of Isolated Adult Rat Heart Cells: The Surface Morphology and the Influence of Extracellular Calcium Ion Concentration on Cellular Viability ,
Tissue Cell
 9 (3) , 419 , 1977
Cells
Myocytes
Enzymes
Hyaluronidase : 0.1%
Medium
Kreb's Ringers phosphate
Heart
Rat
Rat, adult, male, 150-200g
Grosso, D. , Frangakis, C. , Carlson, E. and Bressler, R.
Isolation and Characterization of Myocytes From the Adult Rat Heart ,
Prep Biochem
 7 (5) , 383 , 1977
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Medium
MEM CF
Heart
Rat
Rat, SD, male, 150-200 g
Pretlow II, T. , Glick, M. and Reddy, W.
Separation of Beating Cardiac Myocytes from Suspensions of Heart Cells ,
Am J Pathol
 67 (2) , 215 , 1972
Cells
Myocytes
Enzymes
Hyaluronidase : 0.05%
Medium
(see reference)
Heart
Rat
Rat, albino, male, 250 g
Berry, M. , Friend, D. and Scheuer, J.
Morphology and Metabolism of Intact Muscle Cells Isolated from Adult Rat Heart ,
Circ Res
 26 , 679 , 1970
Cells
Myocytes
Enzymes
Hyaluronidase : 0.20%
Medium
Hank's solution, CF
Intestine
Mouse
Mouse, embryonic
Ren HJ, Zhang CL, Liu RD, Li N, Li XG, Xue HK, Guo Y, Wang ZQ, Cui J. and Ming L
Primary Cultures of Mouse Small Intestinal Epithelial Cells using the Dissociating Enzyme Type I Collagenase and Hyaluronidase. ,
Braz J Med Biol Res
 50 , e5831 , 2017
Cells
Crypt
Enzymes
Collagenase Type 1 : 200 u/ml
Hyaluronidase : 100u/ml
Medium
DMEM
Kidney
Bovine
Bovine
Husted, R. , Hayashi, M. and Stokes, J.
Characteristics of Papillary Collecting Duct Cells in Primary Culture ,
Am J Physiol
 255 , F1160 , 1988
Cells
Papillary duct
Enzymes
Hyaluronidase : 0.2%
Medium
Keri's buffer
HEPES buffered saline
Kidney
Hamster
Hamster
(also rat, SD and Wistar, 150-225 g, rabbit, bovine)
Husted, R. , Hayashi, M. and Stokes, J.
Characteristics of Papillary Collecting Duct Cells in Primary Culture ,
Am J Physiol
 255 , F1160 , 1988
Cells
Papillary duct
Enzymes
Hyaluronidase : 0.2%
Medium
Keri's buffer
HEPES buffered saline
Kidney
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Kidney
Rabbit
Rabbit
(also rat, SD and Wistar, 150-225 g, hamster, bovine)
Husted, R. , Hayashi, M. and Stokes, J.
Characteristics of Papillary Collecting Duct Cells in Primary Culture ,
Am J Physiol
 255 , F1160 , 1988
Cells
Papillary duct
Enzymes
Hyaluronidase : 0.2%
Medium
Keri's buffer
HEPES buffered saline

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.