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Tissue References Search Tool

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Reproductive
Human
Human
Fahey John V, Wira Charles R.
Effect of menstrual status on antibacterial activity and secretory leukocyte protease inhibitor production by human uterine epithelial cells in culture ,
J Infect Dis
 185 , 1606-13 , 2002
Cells
Uterine epithelial cells
Enzymes
Pancreatin : 0.34%
Collagenase : 0.16%
Hyaluronidase : 0.16%
Medium
HBSS
Reproductive
Human
Human
Yusuf RZ, Naeem R.
Cytogenetic studies of spontaneous miscarriages: a seven year study to compare significance of primary vs. secondary culture methods for assessment of fetal karyotype yield and maternal cell contamination ,
Early Pregnancy
 5 , 121-31 , 2001
Cells
Chorionic villi
Enzymes
Trypsin : see reference
Collagenase Type 3 : 100 u/ml
Medium
HBSS
Reproductive
Human
Human, female, 20-40 year
Gargett CE, Bucak K, Rogers PA.
Isolation, characterization and long-term culture of human myometrial microvascular endothelial cells ,
Hum Reprod
 15 , 293-301 , 2000
Cells
Microvascular endothelial cells
Enzymes
Medium
PBS
Reproductive
Human
Human
Runesson E, Ivarsson K, Janson P, Brannstrom M.
Gonadotropin- and cytokine-regulated expression of the chemokine interleukin 8 in the human preovulatory follicle of the menstrual cycle ,
J Clin Endocrinol Metab
 85 , 4387-95 , 2000
Cells
Theca cells
Enzymes
Medium
PBS
Reproductive
Human
Human
Branchaud, C.L. , Goodyer, C.G. , Guyda, H.J. and Lefebvre, Y.
A Serum-Free System for Culturing Human Placental Trophoblasts ,
In Vitro Cell Dev Biol
 26 , 865 , 1990
Cells
Trophoblasts, placental
Enzymes
Trypsin : 0.25%
Medium
EBSS, CMF
Reproductive
Human
Human
Jie, Z. , Fey, S. , Hager, H. , Hollsberg, P. , Ebbesen, P. and Larsen, P.
Markers For Human Placental Trophoblasts in Two-Dimensional Gel Electrophoresis ,
In Vitro Cell Dev Biol
 26 , 937 , 1990
Cells
Trophoblasts, placental
Enzymes
Trypsin : 0.25%
Medium
PBS
Reproductive
Human
Human
Morrish, D. and Siy, O.
Critical Factors in Establishing Monolayer Cultures of Normal Human Placental Cells in Serum-Free Medium ,
Endocr Res
 12 (3) , 229 , 1986
Cells
Placental
Enzymes
Trypsin : 0.25%
Medium
DMEM
Reproductive
Human
Human, female, 27-49 years
Auersperg, N. , Siemens, C.H. and Myrdal, S.E.
Human Ovarian Surface Epithelium In Primary Culture ,
In Vitro
 20 , 743 , 1984
Cells
Epithelial
Ovary
Enzymes
Trypsin : 0.125%
Medium
HBSS, CMF
Reproductive
Human
Human, infant and neonate
Lechner, J. , Babcock, M. , Marnell, M. , Narayan, K. and Kaighn, M.
Normal Human Prostate Epithelial Cell Cultures ,
Methods Cell Biol
 21 , 195 , 1980
Cells
Epithelial
Prostate
Enzymes
Trypsin : 0.1%
Medium
HBSS
Reproductive
Human
Human, female, 27 years
Rifas, L. , Fant, J. , Makman, M. and Seifter, S.
The Characterization of Human Uterine Smooth Muscle Cells in Culture ,
Cell Tissue Res
 196 , 385 , 1979
Cells
Smooth muscle, uterine
Enzymes
Trypsin : 0.05%
Medium
EBSS
Reproductive
Insect
Drosophila, embryonic
Salmand, P. Iche-Torres, M. and Perrin, L.
Tissue-Specific Cell Sorting from Drosophila Embryos: Application to Gene Expression Analysis. , Fly (Austin) Vol. 5 , 261
Cells
Cardiac differentiating
Enzymes
Collagenase Type 1 : 20 u/ml
Trypsin : 0.25%
Medium
Schneider
Reproductive
Mouse
Mouse, adult
Lee, A, Rusch, J. , Lima, A. , Usmani, A. , Huang, N. , Lepamets, M. , Vigh-Conrad, K. , Worthington, R. and et al.
Rare Mutations in the Complement Regulatory Gene CSMD1 are Associated with Male and Female Infertility. ,
Nat Commun
 10 , 4626 , 2019
Cells
Testes
Enzymes
Medium
MEM
Reproductive
Mouse
Mouse, adult
Martin, L. and Seandel, M.
Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-Term Adult Mouse SSC Lines. ,
J Vis Exp
 72 , e50017 , 2013
Cells
Spermatogonial stem
Enzymes
Trypsin : 0.05%
Medium
DMEM
Reproductive
Mouse
Mouse, male
Getun, I. , Torres, B. and Bois, P.
Flow Cytometry Purification of Mouse Meiotic Cells. ,
J Vis Exp
 50 , 2602 , 2011
Cells
Testis, meiotic
Enzymes
Collagenase Type 1 : 120 u/ml
Trypsin : 0.1%
Medium
Gey's BSS
Reproductive
Mouse
Mouse, 6 day
Nalbandian Angele, Dettin Luis, Dym Martin, Ravindranath Neelakanta.
Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse ,
Biol Reprod
 69 , 985-94 , 2003
Cells
Sertoli
Enzymes
Medium
DMEM
Reproductive
Mouse
Mouse, male, 12-14-week-old
Lin, Q. , Sirotkin, A. and Skoultchi, A.
Normal Spermatogenesis in Mice Lacking the Testis-Specific Linker Histone H1t ,
Mol Cell Biol
 20 (6) , 2122 , 2000
Cells
Seminiferous tubules
Enzymes
Trypsin : 0.05%
Medium
DMEM
Reproductive
Mouse
Mouse, female,6-8 weeks
Ghosh, D. , Danielson, K. , Alston, J. and Heyner, S.
Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes ,
In Vitro Cell Dev Biol
 27A , 713 , 1991
Cells
Uterine
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
HBSS
Reproductive
Mouse
Mouse, CF1, female
Spindle, A.
In vitro Development of One-Cell Embryos from Outbred Mice: Influence of Culture Medium Composition ,
In Vitro Cell Dev Biol
 26 , 151 , 1990
Cells
Cumulus, one-cell embryos
Enzymes
Hyaluronidase : 0.1%
Medium
PBS, CMF
Reproductive
Mouse
Mouse (BALB/cCRGL), male, 2-3 months
Turner, T. , Bern, H. , Young,P. and Cunha, G.
Serum-Free Culture of Enriched Mouse Anterior and Ventral Prostatic Epithelial Cells in Collagen Gel ,
In Vitro Cell Dev Biol
 26 , 722 , 1990
Cells
Epithelial, prostate gland
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Reproductive
Mouse
Mouse (C57B/T), fetus, 16-17 days old
Thompson, T.C. , Southgate, J. , Kitchener, G. and Land, H.
Multistage Carcinogenesis Induced by ras and myc Oncogenes in a Reconstituted Organ ,
Cell
 56 , 917 , 1989
Cells
Prostate
Enzymes
Trypsin : 1.0%
Medium
HBSS/ DMEM
Reproductive
Mouse
Mouse, outbred, CD-1, 21-23 days old
Tomooka, Y. , DiAugustine, R. and McLachlan, J.
Proliferation of Mouse Uterine Epithelial Cells in Vitro ,
Endocrinology
 118 (3) , 1011 , 1986
Cells
Epithelial
Enzymes
Trypsin : 0.5%
Medium
Medium 199
Reproductive
Mouse
Mouse, BALB/c
Bigsby, R. , Cooke, P. and Cunha, G.
A Simple Efficient Method For Separating Murine Uterine Epithelial and Mesenchymal Cells ,
Am J Physiol
 251 , E630 , 1986
Cells
Epithelial
Mesencymal
Enzymes
Trypsin : 1%
Medium
DMEM
Reproductive
Mouse
Mouse, BALB/cCrg, 40 days old
Cooke, P. , Uchima, F. , Fujii, D. , Bern, H. and Cunha, G.
Restoration of Normal Morphology and Estrogen Responsiveness in Cultured Vaginal and Uterine Epithelia Transplanted with Stroma ,
Proc Natl Acad Sci U S A
 83 , 2109 , 1986
Cells
Epithelial
Enzymes
Trypsin : 1%
Medium
Medium 199
Reproductive
Porcine
Porcine, 1-4 day
Park, M. , Park, J. , Kim, M. , Lee, K. , Park, H. , Yun, J. , Choi, J. , Lee, E. and Lee, S.
Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes. ,
J Assist Reprod Genet
 31 , 983-91 , 2014
Cells
Testicular
Enzymes
Hyaluronidase : 0.1%
Trypsin : 0.25%
Medium
DMEM
Reproductive
Porcine
Porcine, male, 8 day
Dirami G, Ravindranath N, Pursel V, Dym M.
Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM ,
Biol Reprod
 61 , 225-30 , 1999
Cells
Seminiferous epithelial cells
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.15%
Trypsin : 0.05%
Medium
DMEM/F12
Reproductive
Porcine
Porcine, 3-4 week
Mather, J. , Saez, J. and Haour, F.
Regulaton of Gonadotropin Receptors and Steroidogenesis in Cultured Porcine Leydig Cells ,
Endocrinology
 110 , 933 , 1982
Cells
Leydig
Testis
Enzymes
Trypsin : 0.0003%
Medium
Lebovitz L-15 Medium
Reproductive
Rabbit
Rabbit, New Zealand, 3 month
Kubota, H. , Wu, X. , Goodyear, S. , Avarbock, M. and Brinster, R.
Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties. ,
FASEB J
 25 , 2604 , 2011
Cells
Testicular germ
Enzymes
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand, 3 month
Kubota, H. , Wu, X. , Goodyear, S. , Avarbock, M. and Brinster, R.
Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties. ,
FASEB J
 25 , 2604 , 2011
Cells
Testicular germ
Enzymes
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand white, mature, 3-4 Kg
Boulet, A. and Fortier, M.
Preparation and Characterization of Rabbit Myometrial Cells in Primary Culture: Influence of Estradiol and Progesterone Treatment ,
In Vitro Cell Dev Biol
 23 , 93 , 1987
Cells
Myometrial
Enzymes
Trypsin : 0.02%, 0.03%, 0.0375%
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand white estrous, female, 4-5 months
Nicosia, S. , Johnson, J. and Streibel, E.
Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium) ,
Int J Gynecol Pathol
 3 , 348 , 1984
Cells
Mesothelial and surface epithelial
Ovaries
Enzymes
Trypsin : 0.125%-0.5%
Medium
Medium 199
Reproductive
Rat
Rat, SD, adult, male, 8 - 10 week
Abou-Haila, A. and Tulsiani, D.
Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization ,
Biol Proced Online
 3 (1) , 35 , 2001
Cells
Seminiferous tubules
Enzymes
Trypsin : 0.05%
Medium
Krebs-Ringer bicarbonate buffer
(see reference)
Reproductive
Rat
Rat, Han-Wistar, adult, male, 250 g
Leckie, C. , Welberg, L. and Seckl, J.
11B-Hydroxysteroid Dehydrogenase is a Predominant Reductase in Intact Rat Leydig Cells ,
J Endocrinol
 159 , 233 , 1998
Cells
Testes
Enzymes
Trypsin : 0.1%
Medium
Medium 199 w/ Hank's salts
Reproductive
Rat
Rat, SD, male, 90 days
Onoda, M. and Djakiew, D.
Pachytene Spermatocyte Protein(s) Stimulate Sertoli Cells Grown in Bicameral Chambers: Dose-Dependent Secretion of Ceruloplasmin, Sulfated Glycoprotein-1, Sulfated Glycoprotein-2, and Transferrin ,
In Vitro Cell Dev Biol
 27 , 215 , 1991
Cells
Sertoli
Enzymes
Hyaluronidase : 0.1%
Medium
DMEM
Reproductive
Rat
Rat, SD, male
Abayasekara, D. , Kurlak, L. , Band, A. , Sullivan, M. and Cooke, B.
Effect of Cell Purity, Cell Concentration, and Incubation Conditions on Rat Testis Leydig Cell Steroidogenesis ,
In Vitro Cell Dev Biol
 27 , 253 , 1991
Cells
Leydig
Enzymes
Trypsin : 0.02%
Medium
DMEM
Reproductive
Rat
Rat, neonatal
Branham, W. , Lyn-Cook, B. , Andrews, A. , McDaniel, M. and Sheehan, D.
Growth of Neonatal Rat Uterine Luminal Epithelium on Extracellular Matrix ,
In Vitro Cell Dev Biol
 27 , 442 , 1991
Cells
Uterine
Enzymes
Trypsin : 1%
Medium
HBSS
Reproductive
Rat
Rat, Wistar, adult
Pampfer, S. , Vanderheyden, I. , Michiels, B. and DeHertogh, R.
Co-Culture of Two-Cell Rat Embryos on Cell Monolayers ,
In Vitro Cell Dev Biol
 26 , 944 , 1990
Cells
Uterine
Enzymes
Trypsin : 0.5%
Medium
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.