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Tissue References Search Tool

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Brain
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Brain
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Brain
Human
Human
Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.
Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009
Cells
Tumor
Enzymes
Medium
see reference
Liver
Avian
Ducklings, 7 day
Lee J. , Culvenor J. , Angus P. , Smallwood R. , Nicoll A. and Locarnini S.
Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells ,
J Virol
 75(16) , 7651 , 2001
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Hyaluronidase : 0.05%
Medium
DMEM/ F12
Liver
Fish
Trout 100g - 5kg
Bailey, G. , Taylor, M. and Selivonchick, D.
Aflatoxin B1 Metabolism and DNA Binding in Isolated Hepatocytes From Rainbow Trout Salmo gairdner ,
Carcinogenesis
 3 , 511 , 1982
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.08%
Medium
See reference
Liver
Human
Human, fetal
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Cells
Liver hematopoietic
Enzymes
Medium
RPMI
Liver
Mouse
Mouse, 20-30 g
Crisp, D. and Pogson, C.
Glycolytic and Gluconeogenic Enzyme Activities in Parenchymal and Non-parenchymal Cells from Mouse Liver ,
Biochem J
 126 , 1009 , 1972
Cells
Parenchymal and non-parenchymal
Enzymes
Hyaluronidase : 0.1%
Medium
Hank's w/ Insulin, CMF
Liver
Rat
Rat, SD, 500-750 g
Li, Z. , Dranoff, J. , Chan, E. , Uemura, M. and Sevigny, J.and Wells, R.
Transforming Growth Factor-beta and Substrate Stiffness Regulate Portal Fibroblast Activation in Culture. ,
Hepatol
 46 , 1246-56 , 2007
Cells
Portal fibroblasts
Enzymes
Collagenase : 0.03%
Pronase : 0.033%
Hyaluronidase : 0.036%
Medium
DMEM/F-12
Liver
Rat
Rat, Fisher
Blouin, M. and Marceau, N.
Primary Culture of Fetal Rat Liver Bipotential Progenitor Cells ,
J Tiss Cul Meth
 13 , 117 , 1991
Cells
Epithelial
Enzymes
Collagenase : 0.05%
Hyaluronidase : 0.075%
Medium
Eagles's MEM
Liver
Rat
Rat, Wistar, male, 180-210 g
De Gerlache, J. , Lans, M. , Taper, H. and Roberfroid, M.
Separate Isolation of Cells from Nodules and Surrounding Parenchyma of the Same Precancerous Rat Liver: Biochemical and Cytochemical Characterization ,
Toxicology
 18 , 225 , 1980
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.02%
Medium
Kreb's buffer
Liver
Rat
Rat, Wistar, male, 200-250 g
Poli, G. , Gravela, E. , Albano, E. and Dianzani, M.
Studies on Fatty Liver with Isolated Hepatocytes: II., The Action of Carbon Tetrachloride on Lipid Peroxidation, Protein and Triglyceride Synthesis and Secretion ,
Exp Mol Pathol
 30 , 116 , 1979
Cells
Hepatocytes
Enzymes
Hyaluronidase : 460 u/ml
Medium
Saline
Liver
Rat
Rat, embryos, 1-3 days
Gerschenson, L. , Berliner, J. and Davidson, M.
The Isolation and Culture of Liver Cells , Vol. 32 , 733 , 1974
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.10%
Medium
HBSS
Liver
Rat
Rat, Fischer, adult, 200-250 g
Williams, G. and Gunn, J.
Long-Term Culture of Adult Rat Liver Epithelial Cells ,
Exp Cell Res
 89 , 139 , 1974
Cells
Epithelial
Enzymes
Hyaluronidase : 0.0075%
Medium
KCl-NaCl HEPES Buffer
Liver
Rat
Rat, SD, albino, male
Bonney, R. , Becker, J. , Walker, P. and Potter, V.
Primary Monolayer Cultures of Adult Rat Liver Parenchymal Cells Suitable for Study of the Regulation of Enzyme Synthesis ,
In Vitro
 9 , 399 , 1974
Cells
Parenchymal
Enzymes
Hyaluronidase : 0.1%
Medium
Hank's solution, CMF
Liver
Rat
Rat, SD, adult, male, 200-250 g
Howard, R. , Lee, J. and Pesch, L.
The fine structure, potassium content, and respiratory activity of isolated rat liver parenchymal cells prepared by improved enzymatic techniques ,
J Cell Biol
 57 , 642 , 1973
Cells
Parenchymal
Enzymes
Hyaluronidase : 0.10%
Medium
HBSS, CF
Liver
Rat
Rat, Wistar, 200-250 g
Berg, T. , Boman, D. and Seglen, P.O.
Induction of Tryptophan Oxygenase in Primary Rat Liver Cell Suspensions by Glucocorticoid Hormone ,
Exp Cell Res
 72 , 571 , 1972
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.10%
Medium
HBSS, CF
Liver
Rat
Rat, SD, female, 130-160 g
Johnson, M. , Das, N. , Butcher, F. and Fain, J.
The Regulation of Gluconeogenesis in Isolated Rat Liver Cells by Glucagon, Insulin, Dibutyryl Cyclic Adenosine Monophosphate, and Fatty Acids ,
J Biol Chem
 247 , 3229 , 1972
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.08%
Medium
HBSS, CF
Liver
Rat
Rat, Wistar, male, 6-8 weeks, 80-160 G
Iype, P.
Cultures from Adult Rat Liver Cells. 1.Establishment of Monolayer Cell-Cultures from Normal Lver ,
J Cell Physiol
 78 , 281 , 1971
Cells
Hepatocytes
Enzymes
Hyaluronidase : 1.0%
Medium
HBSS, CMF
Liver
Rat
Rat, 100-200 g
Haung, Y. and Ebner, K.
Induction of Tyrosine Aminotransferase in Isolated Liver Cells ,
Biochim Biophys Acta
 191 , 161 , 1969
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.10%
Medium
HBSS, CF
Liver
Rat
Rat, SD, adult, 200-300 g
Berry, M. and Friend, D.
High Yield Preparation of Isolated Rat Liver Parenchymal Cells ,
J Cell Biol
 43 , 506 , 1969
Cells
Parenchymal
Enzymes
Medium
HBSS, CF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.