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Tissue References Search Tool

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Epithelial
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Epithelial
Mouse
Mouse, female
Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y.
Estrogen deficiency accelerates autoimmune exocrinopathy in murine Sjogren's syndrome through fas-mediated apoptosis ,
Am J Pathol
 155 , 173-81 , 1999
Cells
Salivary gland epithelial
Enzymes
Collagenase Type 1 : 750 u/ml
Hyaluronidase : 500 u/ml
Medium
DMEM/F12
Epithelial
Rat
Rat, 4-14 week
Wang N, Koutz CA. and Anderson RE.
A method for the isolation of retinal pigment epithelial cells from adult rats ,
Inv Ophthal Visual Sci
 34(1) , 101 , 1993
Cells
Retinal pigment epithelial cells
Enzymes
Collagenase Type 1 : 65 u/ml
Hyaluronidase : 220 u/ml
Medium
CF Hanks with EDTA
Epithelial
Rat
Rat, Fischer, male, 4-6 weeks
Babcock, M. , Marino, M. , Gunning, W. and Stoner, G.
Clonal Growth and Serial Propagation of Rat Esophageal Epithelial Cells ,
In Vitro
 19 , 403 , 1983
Cells
Epithelial
Esophagus
Enzymes
Hyaluronidase : 0.1%
Medium
HEPES BSS
Epithelial
Rat
Rat, Fischer, adult, 200-250 g
Williams, G. and Gunn, J.
Long-Term Culture of Adult Rat Liver Epithelial Cells ,
Exp Cell Res
 89 , 139 , 1974
Cells
Epithelial
Enzymes
Hyaluronidase : 0.0075%
Medium
KCl-NaCl HEPES Buffer
Pancreas
Guinea-Pig
Guinea-pig
Amsterdam, J. and Jamieson, J.
Structural and Functional Characterization of Isolated Pancreatic Exocrine Cells ,
Proc Natl Acad Sci U S A
 69 (10) , 3028 , 1972
Cells
Exocrine
Enzymes
Hyaluronidase : 0.15% - 0.2%
Medium
Kreb's Ringer
Pancreas
Monkey
Monkey, 3-5 Kg
Scharp, D. , Murphy, J. , Newton, W. , Ballinger, W. and Lacy, P.
Application of an Improved Isolation Technique for Islet Transplantation in Primates and Rats ,
Transplant Proc
 7 , 739 , 1975
Cells
Islets
Enzymes
Hyaluronidase : 0.05%
Medium
HBSS
Pancreas
Mouse
Mouse, 6-10 month
Wang, Y. , Soyombo, A. , Shcheynikov, N. , Zeng, W. , Dorwart, M. , Marino, C. , Thomas, P. and Muallem, S.
Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis. ,
EMBO J
 25 , 5049 , 2006
Cells
Pancreatic ductal
Enzymes
CLSPA : 50 u/ml
Hyaluronidase : 400 u/ml
Medium
DMEM
Pancreas
Mouse
Mouse (C57BL/6J-ob/ob), 9-12 wks, male
Dalpe-Scott, M. , Heick, H. and Begin-Heick, N.
Secretion in the Obese (ob/ob) Mouse. The Effect of Oxytetracycline on Insulin Release ,
Diabetes
 32 , 932 , 1983
Cells
Islets
Enzymes
Hyaluronidase : 0.5%
Medium
Kreb's Ringer bicarbonate buffer
Pancreas
Rabbit
Rabbit, New Zealand white, Male/Female, 2-3 Kg
Renckens, B. , Schrijen, J. , Swarts, H. , DePont, J. and Bonting, S.
Role of Calcium in Exocrine Pancreatic Secretion. IV. Calcium Movements in Isolated Acinar Cells of Rabbit Pancreas ,
Biochim Biophys Acta
 544 , 338 , 1978
Cells
Acinar
Enzymes
Hyaluronidase : 0.2%
Medium
Kreb's Ringer bicarbonate buffer
Pancreas
Rat
Rat, SD, male, 40 - 100 g
Hirschi, K. , Kenny, S. , Justice, J. and Brannon, P.
Effects of Secretin And Caerulein On Enzymes Of Cultured Pancreatic Acinar Cells ,
In Vitro Cell Dev Biol
 27 , 660 , 1991
Cells
Acinar
Enzymes
Hyaluronidase : 462 u/ml
Medium
Ham's F12
Pancreas
Rat
Rat, Wistar, male, 250 - 350 g
Melvin, J. , Kawaguchi, M. , Baum, B. and Turner, R.
A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells ,
Biochem Biophys Res Commun
 145 , 754 , 1987
Cells
Acinar, parotid
Enzymes
Hyaluronidase : 0.015%
Medium
Earle's MEM
Pancreas
Rat
Rat, SD, male, 42 - 48 day, 175 - 200 g
Quissell, D. , Redman, R. and Mark, M.
Short-Term Primary Culture of Acinar-Intercalated Duct Complexes From Rat Submandibular Glands ,
In Vitro Cell Dev Biol
 22 , 469 , 1986
Cells
Acinar, submandibular gland
Enzymes
Hyaluronidase : 0.1 %
Medium
HBSS, CF
Pancreas
Rat
Rat, SD, male, 50-75 g
Brannon, P. , Orrison, B. and Kretchmer, N.
Primary Cultures of Rat Pancreatic Acinar Cells in Serum-Free Medium ,
In Vitro Cell Dev Biol
 21 (1) , 6 , 1985
Cells
Acinar
Enzymes
Hyaluronidase : 0.1%
Medium
HBSS
(see reference)
Pancreas
Rat
Rat
Schulz, I. , Heil, K. , Kribben, A. , Sachs, G. and Haase, W.
Isolation and Functional Characterization of Cells From the Exocrine Pancreas , Biology of Normal and Cancerous Exocrine Pancreatic Cells , Ribet, A., Pradayrol, L., and Susini, C. , Elsevier , 1980
Cells
Exocrine
Enzymes
Hyaluronidase : 0.9%
Medium
Kreb's Ringer
Pancreas
Rat
Rat, SD, male, 250 - 350 g
(also mouse, white, Swiss, male, 20 - 24 g)
Williams, J. , Korc, M. and Dormer, R.
Action of Secretagogues on a New Preparation of Functionally Intact, Isolated Pancreatic Acini ,
Am J Physiol
 235 , 517 , 1978
Cells
Acinar
Enzymes
Hyaluronidase : 0.18%
Medium
Kreb's Henseleit bicarbonate buffer
Pancreas
Rat
Rat
Kondo, S. and Schulz, I.
Calcium Ion Uptake in Isolated Pancreas Cells Induced by Secretagogues ,
Biochim Biophys Acta
 419 , 76 , 1976
Cells
Exocrine
Enzymes
Hyaluronidase : 0.15%
Medium
Krebs
Pancreas
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.