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Tissue References Search Tool

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Neural
Rat
Rat, SD, embryonic
Bixby, S. , Kruger, G. , Mosher, J. , Joseph, N. and Morrison, S.
Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity. ,
Neuron
 35 , 643 , 2002
Cells
Sciatic nerve and gut neural crest stem
Enzymes
Trypsin : 0.005%
Medium
HBSS
Neural
Rat
Rat, newborn, 7 days old
Acosta, C. , Fabrega, A. , Masco, D. and Lopez, H.
A Sensory Neuron Subpopulation with Unique Sequential Survival Dependence on Nerve Growth Factor and Basic Fibroblast Growth Factor during Development ,
J Neurosci
 21 (22) , 8873 , 2001
Cells
Neurons
Enzymes
Trypsin : 0.25%
Medium
MEM10
Neural
Rat
Rat
(also mice)
Johansson, C. , Momma, S. , Clarke, D. , Risling, M. , Lendahl, U. and Frisen, J
Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System ,
Cell
 96 , 25 , 1999
Cells
Spinal cord
Enzymes
Trypsin : 0.133%
Medium
HBSS and PIPES
Neural
Rat
Rat, SD, female, pregnant
Morrison, S. , White, P. , Zock, C. and Anderson, D.
Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells ,
Cell
 96 , 737 , 1999
Cells
Sciatic nerves
Enzymes
Trypsin : 0.025%
Medium
L-15 medium
(see reference)
Neural
Rat
Rat, SD, embryonic
Morrison, S. , White, P. , Zock, C. and Anderson, D.
Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells ,
Cell
 96 , 737 , 1999
Cells
Sciatic nerves
Enzymes
Trypsin : 0.025%
Medium
L-15 medium
(see reference)
Neural
Rat
Rat, Wister and SD, newborn, 0-21 days, either sex
Schafer, K. , Saffrey, M. , Burnstock, G. and Mastres-Ventura, P.
A New Method for the Isolation of Myenteric Plexus from the Newborn Rat Gastrointestinal Tract ,
Brain Res Proto
 1 , 109 , 1997
Cells
Myenteric ganglia
Enzymes
Trypsin : 0.05%
Medium
MEM-HEPES
Neural
Rat
Rats, SD, 11-14 day old, 29-32 g
Allen, T. , Sim, J. and Brown, D.
The Whole-Cell Calcium Current in Acutely Dissociated Magnocellular Cholinergic Basal Forebrain Neurones of the Rat ,
J Physiol
 460 , 91 , 1993
Cells
Basal forebrain neurons
Enzymes
Trypsin : 0.125%
Medium
Gey's BSS
Neural
Rat
Rat, Wistar-Hanover, 7-12 day
Rayport, S. , Sulzer, D. , Shi, W. , Sawasdikosol, S. , Monaco, J. , Batson, D. and Rajendran, G.
Identified Postnatal Mesolimbic Dopamine Neurons in Culture: Morphology and Electrophysiology ,
J Neurosci
 12 (11) , 4264 , 1992
Cells
Postnatal dopamine neurons
Enzymes
Trypsin : 0.035%
Medium
(see reference)
Neural
Rat
Rat, SD, 18-day-old, fetus
Cheng, B. and Mattson, M.
IGF-I and IGF-II Protect Cultured Hippocampal and Septal Neurons Against Calcium-Mediated Hypoglycemic Damage ,
J Neurosci
 12 (4) , 1558 , 1992
Cells
Hippocampal
Enzymes
Trypsin : 0.2%
Medium
HBSS
Neural
Rat
Rat, SD, pregnant, Charles River
Buchhalter, J. and Dichter, M.
Electrophysiological Comparison of Pyramidal and Stellate Nonpyramidal Neurons in Dissociated Cell Culture of Rat Hippocampus ,
Brain Res Bull
 26 , 333 , 1991
Cells
Pyramidal neurons
Nonpyramidal neurons
Enzymes
Trypsin : 0.027%
Medium
HEPES
Neural
Rat
Rat, SD, embryos, 19-21 days gestation
Mithen, F. , Reiker, M. and Birchem, R.
Effects of Ethanol on Rat Schwann Cell Proliferation and Myelination in Culture ,
In Vitro Cell Dev Biol
 26 , 129 , 1990
Cells
Schwann, dorsal root ganglia
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Neural
Rat
Rat
(also bovine)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Neural
Rat
Rat, SD, 8 day old pups
Novelli, A. , Reilly, J. , Lysko, P. and Henneberry, R.
Glutamate Becomes Neurotoxic Via the N-methyl-D-aspartate Receptor When Intracellular Energy Levels are Reduced ,
Brain Res
 451 , 205 , 1988
Cells
Cerebellar neurons
Enzymes
Trypsin : 0.025%
Medium
Eagle's MEM
Neural
Rat
Rat, fetuses, 18 day old
Mattson, M. and Kater, S.
Isolated Hippocampal Neurons in Cryopreserved Long-Term Cultures: Development of Neuroarchitecture and Sensitivity to Nmda ,
Int J Dev Neurosci
 6 (5) , 439 , 1988
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.2%
Medium
Eagle's MEM
Neural
Rat
Rat, SD, female, 4 month old
Wood, P. and Bunge, R.
Evidence That Axons are Mitogenic for Oligodendrocytes Isolated From Adult Animals ,
Nature
 320 , 756 , 1986
Cells
CNS cells
Enzymes
Trypsin : 0.25%
Medium
EBSS
Neural
Rat
Rat, embryo, 15 day
Jahr, C. and Jessell, T.
Synaptic Transmission between Dorsal Root Ganglion and Dorsal Horn Neurons in Culture: Antagonism of Monosynaptic Excitatory Postsynaptic Potentials and Glutamate Excitation by Kynurenate ,
J Neurosci
 5 , 2281 , 1985
Cells
Dorsal horn neurons
Spinal
Enzymes
Trypsin : 0.025%
Medium
Ham's F-12
Neural
Rat
Rat, fetus, 18-20 day
Bartlett, W. and Banker, G.
An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts ,
J Neurosci
 4 , 1944 , 1984
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Neural
Rat
Rat, SD, pups
Sarthy PV, Curtis BM. and Catterall WA.
Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat ,
J Neurosci
 3 (12) , 2532 , 1983
Cells
Retina
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Neural
Rat
Rat, W/FU, 5-8 day
Raff, M. , Fields, K. , Hakomori, S. , Mirsky, R. , Pruss, R. and Winter, J.
Cell-Type-Specific Markers for Distinguishing and Studying Neurons And the Major Classes of Glial Cells in Culture ,
Brain Res
 174 , 283 , 1979
Cells
Neurons and glial
Enzymes
Trypsin : 0.25%
Medium
MEM
(see reference)
Neural
Rat
Rat, fetus
Wakshull, E. , Johnson, M. and Burton, H.
Postnatal Rat Sympathetic Neurons In Culture. 1. A Comparison With Embryonic Neurons ,
J Neurophysiol
 42 , 1410 , 1979
Cells
Neurons, sympathetic
Enzymes
Trypsin : 0.25%
Medium
L-15 or HBSS, CMF
Neural
Rat
Rat, Wistar/Furth, newborn
(also bovine)
Brockes, J. , Fields, K. and Raff, M.
Studies on Cultured Rat Schwann Cells. I. Establishment of Purified Populations From Cultures of Peripheral Nerve ,
Brain Res
 165 , 105 , 1979
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, embryo
Dichter, M.
Rat Cortical Neurons in Cell Culture: Culture Methods, Cell Morphology, Electrophysiology, and Synapse Formation ,
Brain Res
 149 , 279 , 1978
Cells
Neurons, cortical
Enzymes
Trypsin : 0.027%
Medium
MEM
Neural
Rat
Rat, neonatal
Mains, R. and Patterson, P.
Primary Cultures of Dissociated Sympathetic Neurons I. Establishment of Long-Term Growth in Culture and Studies of Differentiated Properties ,
J Cell Biol
 59 , 329 , 1973
Cells
Neurons, superior cervical ganglia
Enzymes
Trypsin : 0.1%
Medium
Basal L-15 medium
Neural
Shellfish
Snails (Helisoma trivolis), albino, adult
Zoran, M. , Doyle, R. and Haydon, P.
Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis ,
Neuron
 6 , 145 , 1991
Cells
Buccal ganglia; SLT muscle
Enzymes
Trypsin : 0.2%
Medium
DMEM
Neural
Shellfish
Pond snail (Helisoma) albino, adult
Haydon, P.
The Formation of Chemical Synapses Between Cell-Cultured Neuronal Somata ,
J Neurosci
 8 , 1032 , 1988
Cells
Somata, buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
Antibiotic saline, Leibowitz 50%
Neural
Shellfish
Helisoma trivovlis
Cohan, C. , Haydon, P. and Kater, S.
Single Channel Activity Differs in Growing and Nongrowing Growth Cones of Isolated Identified Neurons of Helisoma ,
J Neurosci Res
 13 , 285 , 1985
Cells
Buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
L-15 medium
Organoids
Mouse

Mouse

Charifou, E. , Sumbal, J. , Koledova, Z. , Li, H. and Chiche, A.
A Robust Mammary Organoid System to Model Lactation and Involution-like Processes. ,
Bio Protoc
 11 , e3996 , 2021
Cells
Mammary organoids
Enzymes
Trypsin : 0.2%
Medium
DMEM/F12
Pancreas
Bovine
Bovine
Stiles, G. and Lefkowitz, R.
Hormone-Sensitive Adneylate Cyclase ,
J Biol Chem
 257 (11) , 6287 , 1982
Cells
Platelets
Medium
(see reference)
Pancreas
Canine
Canine
Zoran, M. , Doyle, R. and Haydon, P.
Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis ,
Neuron
 6 , 145 , 1991
Cells
Buccal ganglia; SLT muscle
Enzymes
Trypsin : 0.2%
Medium
DMEM
Pancreas
Human
Human
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Pancreas
Rat
Rat, SD, male, 100 - 150 g
Yeh, C. , Mertz, P. , Oliver, C. , Baum, B. and Kousvelari, E.
Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells ,
In Vitro Cell Dev Biol
 27 , 707 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.001%
Medium
F12 medium
Pancreas
Rat
Rat, Wistar, male
Foskett, J. , Roifman, C. and Wong, D.
Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells ,
J Biol Chem
 266 , 2778 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.02%
Medium
Solution B
(See reference)
Pancreas
Rat
Rat, Fischer-344, either sex, 120-150 g
Tsao, M. and Duguid, W.
Establishment of Propagable Epithelial Cell Lines From Normal Adult Rat Pancreas ,
Exp Cell Res
 168 , 365 , 1987
Cells
Epithelial
Enzymes
Trypsin : 0.1%
Medium
Ham's F-12/HBSS
(see reference)
Pancreas
Rat
Rat, SD, male, 50 - 125 g
Oliver, C. , Waters, J. , Tolbert, C. and Kleinman, H.
Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel ,
In Vitro Cell Dev Biol
 23 , 465 , 1987
Cells
Acinar
Exorbital lacrimal, parotid, pancreas
Enzymes
Trypsin : 0.01%
Medium
HBSS, CMF
Pancreas
Rat
Rat, SD, 125 - 350 g
Githens, S. , Holmquist, D. , Whelan, J. and Ruby, J.
Ducts of the Rat Pancreas in Agarose Matrix Culture ,
In Vitro
 16 , 797 , 1980
Cells
Duct
Enzymes
Trypsin : 0.01%
Medium
HBSS
Pancreas
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.