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Tissue References Search Tool

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Liver
Mouse
Mouse, 12 week
Mederacke, I. , Dapito, D. , Affo, S. , Uchinami, H. and Schwabe, R.
High-Yield and High-Purity Isolation of Hepatic Stellate Cells from Normal and Fibrotic Mouse Livers. ,
Nat Protoc
 10 , 305-15 , 2015
Cells
Hepatic stellate
Enzymes
Pronase : 0.04%
Medium
DMEM
Liver
Mouse
Mouse
Rountree, C. , Ding, W. , Dang, H. , Vankirk, C. and Crooks, G.
Isolation of CD133+ Liver Stem Cells for Clonal Expansion. ,
J Vis Exp
 56 , e3183 , 2011
Cells
CD133+ stem
Enzymes
Pronase : 0.05%
Medium
DMEM/F12
Liver
Mouse
Mouse, 10-12 week
Beldi, G. , Wu, Y. , Sun, X. , Imai, M. , Enjyoji, K. , Csizmadia, E. , Candinas, D. , Erb, L. and Robson, S.
Regulated Catalysis of Extracellular Nucleotides by Vascular CD39/ENTPD1 is Required for Liver Regeneration. ,
Gastroenterol
 135 , 1751 , 2008
Cells
Liver sinusoidal endothelial cells
Enzymes
Medium
HEPES
Liver
Mouse
Mouse, 8-10 week
Kotton, D. , Fabian, A. and Mulligan, R.
A Novel Stem-Cell Population in Adult Liver with Potent Hematopoietic-Reconstitution Activity. ,
Blood
 106 , 1574 , 2005
Cells
Liver derived stem cells
Enzymes
Medium
HBSS
Liver
Porcine
Porcine, mini-pig, 13 kg
Meng, F. , Chen, Z. , Han, M. , Hu, X. , He, X. , Liu, Y. , He, W. , Huang, W. , Guo, H. and Zhou, P.
Porcine Hepatocyte Isolation and Reversible Immortalization Mediated by Retroviral Transfer and Site-Specific Recombination. ,
World J Gastroenterol
 16 , 1660 , 2010
Cells
Hepatocytes
Enzymes
Deoxyribonuclease I : see reference
Medium
Williams E
Liver
Porcine
Porcine, adult
Zhou X, Liu L, Kano J, Mukaiyama T. and Tokiwa T.
Isolation and Cultivation of Porcine Hepatocytes for Extracorporeal Artificial Liver Support System ,
Chin Med J
 114 , 946 , 2001
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
MEM
Liver
Rat
Rat, SD
Handy, J. , Saxena, N. , Fu, P. , Lin, S. , Mells, J. , Gupta, N. and Anania, F.
Adiponectin Activation of AMPK Disrupts Leptin-Mediated Hepatic Fibrosis via Suppressors of Cytokine Signaling (SOCS-3). ,
J Cell Biochem
 110 , 1195 , 2010
Cells
Hepatic stellate cells
Enzymes
Pronase : 0.02%
Collagenase : see reference
Medium
Medium 199
Liver
Rat
Rat, Wistar, female
Zvibel, I. , Atias, D. , Phillips, A. , Halpern, Z. and Oren, R.
Thyroid Hormones Induce Activation of Rat Hepatic Stellate Cells Through Increased Expression of p75 Neurotrophin Receptor and Direct Activation of Rho. ,
Lab Invest
 90 , 674 , 2010
Cells
Hepatic stellate cells
Enzymes
Collagenase Type 1 : 0.025-0.1%
Pronase : 0.025-0.13%
Medium
DMEM
Liver
Rat
Rat, SD, 500-750 g
Li, Z. , Dranoff, J. , Chan, E. , Uemura, M. and Sevigny, J.and Wells, R.
Transforming Growth Factor-beta and Substrate Stiffness Regulate Portal Fibroblast Activation in Culture. ,
Hepatol
 46 , 1246-56 , 2007
Cells
Portal fibroblasts
Enzymes
Collagenase : 0.03%
Pronase : 0.033%
Hyaluronidase : 0.036%
Medium
DMEM/F-12
Liver
Rat
Rat, adult
Jensen C. , Jauho E. , Santoni-Rugiu E. , Holmskov U. , Teisner B. , Tygstrup N. and Bisgaard H.
Transit-amplifying Ductular (oval) Cells and Their Hepatocytic Progeny are Characterized by a Novel and Distinctive Expression of Delta-like Protein/preadipocyte Factor 1/fetal Antigen 1 ,
Am J Pathol
 164(4) , 1347 , 2004
Cells
Hepatocytes
Enzymes
Pronase : 1%
Medium
Williams E
Liver
Rat
Rat, Fisher
Blouin, M. and Marceau, N.
Primary Culture of Fetal Rat Liver Bipotential Progenitor Cells ,
J Tiss Cul Meth
 13 , 117 , 1991
Cells
Epithelial
Enzymes
Collagenase : 0.05%
Hyaluronidase : 0.075%
Medium
Eagles's MEM
Liver
Rat
Rat, CD strain, albino, male, 140 -180 g
Braun, L. , Mead, J. , Panzica, M. , Mikumo, R. , Bell, G. and Fausto, N.
Transforming Growth Factor B mRNA Increases During Liver Regeneration:A Possible Paracrine Mechanism Of Growth Regulation ,
Proc Natl Acad Sci U S A
 85 , 1539 , 1988
Cells
Hepatocytes and Nonparenchymal
Enzymes
Pronase : 0.1%
Medium
HEPES buffer with calcium
Liver
Rat
Rat, Wistar, male, 3 month old
Nagelkenke, J. , Barto, K. and VanBerkel, T.
In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells ,
J Biol Chem
 258 , 12221 , 1983
Cells
Endothelial
Kupffer
Parenchymal
Enzymes
Collagenase : 0.05%
Pronase : 0.25%
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.