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Tissue References Search Tool

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Neural
Rat
Rat, embryonic and neonatal
Rheaume, C. , Cai, B. , Wang, J. , Fernandez-Salas, E. , Aoki, K. , Francis, J. and Broide, R.
A Highly Specific Monoclonal Antibody for Botulinum Neurotoxin Type A-Cleaved SNAP25. ,
Toxins
 7 , 2354-70 , 2015
Cells
Cortical neurons, DRG
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Neural
Rat
Rat, SD, embryonic
Loktev, A. and Jackson, P.
Neuropeptide Y Family Receptors Traffic via the Bardet-Biedl Syndrome Pathway to Signal in Neuronal Primary Cilia. ,
Cell Rep
 5 , 1316-29 , 2013
Cells
Hypothalamic neuronal
Enzymes
PDS kit : per instructions
Medium
Neurobasal A
Neural
Rat
Rat, SD, 7 day
Tanaka, S. , Shaikh, I. , Chiocca, E. and Saeki, Y.
The Gs-Linked Receptor GPR3 Inhibits the Proliferation of Cerebellar Granule Cells During Postnatal Development. ,
PLoS ONE
 4 , e5922 , 2009
Cells
Cerebellar granule neurons
Enzymes
PDS kit : per instructions
Medium
PBS
Neural
Rat
Rat, Wistar, 4 day
Obradovic Darja, Gronemeyer Hinrich, Lutz Beat, Rein Theo.
Cross-talk of vitamin D and glucocorticoids in hippocampal cells ,
J Neurochem
 96 , 500-9 , 2006
Cells
Hippocampal neurons
Enzymes
PDS kit : per instructions
Medium
Neurobasal A
Neural
Rat
Rat, SD, neonatal
Lacroix-Fralish, M. , Tawfik, V. , Nutile-McMenemy, N. , Harris, B. and Deleo, J.
Differential Regulation of Neuregulin 1 Expression by Progesterone in Astrocytes and Neurons. ,
Neuron Glia Biol
 2 , 227 , 2006
Cells
astrocytes
Enzymes
PDS kit : per instructions
Medium
DMEM
Neural
Rat
Rat, 2 month
Mothe, A. , Kulbatski, I. , Van Bendegem, R. , Lee, L. , Kobayashi, E. , Keating, A. and Tator, C.
Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells ,
J Histochem Cytochem
 53(10) , 1215 , 2005
Cells
Spinal cord progenitor cells
Hybrid toxins
Enzymes
PDS kit : see reference
Medium
Neurobasal A
Neural
Rat
Rat, SD, E19
Gavva NR, Tamir R, Qu Y, Klionsky L, Zhang TJ, Immke D, Wang J, Zhu D, Vanderah TW, Porreca F, Doherty EM, Norman MH, Wild KD, Bannon AW, Louis JC, Treanor JJ.
AMG 9810 [(E)-3-(4-t-butylphenyl)-N-(2,3-dihydrobenzo[b][1,4] dioxin-6-yl)acrylamide], a novel vanilloid receptor 1 (TRPV1) antagonist with antihyperalgesic properties ,
J Pharmacol Exp Ther
 313 , 474 , 2005
Cells
Dorsal root ganglia
Enzymes
PDS kit : per instructions
Medium
MEM/Ham's F12
Neural
Rat
Rat, fetal
Lovshin JA, Huang Q, Seaberg R, Brubaker PL, Drucker DJ.
Extrahypothalamic expression of the glucagon-like peptide-2 receptor is coupled to reduction of glutamate-induced cell death in cultured hippocampal cells ,
Endocrinology
 145 , 3495 , 2004
Cells
Brainstem and cortical neurons
Enzymes
PDS kit : per instructions
Medium
DMEM
Neural
Rat
Rat, SD, male, 270-330 g
Lin CR, Wu PC, Shih HC, Cheng JT, Lu CY, Chou AK, Yang LC.
Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain ,
Cell Transplant
 11 , 17 , 2002
Cells
Spinal progenitor cells
Enzymes
PDS kit : with modifications
Medium
Neurobasal medium
Neural
Rat
Rat, embryonic 18-19 day
Mabuchi T, Kitagawa K, Kuwabara K, Takasawa K, Ohtsuki T, Xia Z, Storm D, Yanagihara T, Hori M, Matsumoto M.
Phosphorylation of cAMP response element-binding protein in hippocampal neurons as a protective response after exposure to glutamate in vitro and ischemia in vivo ,
J Neurosci
 21 , 9204-13 , 2001
Cells
Hippocampal neurons
Enzymes
PDS kit : see reference
Medium
DMEM
Organoids
Human

Human

Tresenrider, A. , Sridhar, A. , Eldred, K. , Cuschieri, S. , Hoffer, D. , Trapnell, C. and Reh, T.
Single-cell Sequencing of Individual Retinal Organoids Reveals Determinants of Cell Fate Heterogeneity. , bioRxiv , 2023
Cells
Retinal Organoids
Enzymes
PDS kit : see reference
Medium
DMEM
Organoids
Human

Human

Schröter, M. , Wang, C. , Terrigno, M. , Hornauer, P. , Huang, Z. , Jagasia, R. and Hierlemann, A.
Functional Imaging of Brain Organoids Using High-Density Microelectrode Arrays. , MRS Bull Vol. 47 , 530-544 , 2022
Cells
Cerebral organoids
Enzymes
PDS kit : see reference
Medium
PBS
Organoids
Human

Human

Uzquiano, A. , Kedaigle, A. , Pigoni, M. , Paulsen, B. , Adiconis, X. , Kim, K. , Faits, T. , Nagaraja, S. , Antón-Bolaños, N. , Gerhardinger, C. , Tucewicz, A. , Murray, E. , Jin, X. , Buenrostro, J. , Chen, F. , Velasco, S. , Regev, A. , Levin, J. and Arlotta, P.
Proper Acquisition of Cell Class Identity in Organoids Allows Definition of Fate Specification Programs of the Human Cerebral Cortex ,
Cell
 185 , 3770-3788.e27 , 2022
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Xue, Y. , Seiler, M. , Tang, W. , Wang, J. , Delgado, J. , McLelland, B. , Nistor, G. , Keirstead, H. and Browne, A.
Retinal Organoids On-a-Chip: a Micro-Millifluidic Bioreactor for Long-term Organoid Maintenance. ,
Lab Chip
 21 , 3361-3377 , 2021
Cells
Stem cell-derived retinal organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Barbar, L. , Rusielewicz, T. , Zimmer, M. , Kalpana, K. and Fossati, V.
Isolation of Human CD49f Astrocytes and iPSC-Based Neurotoxicity Assays. , STAR Protoc Vol. 1 , 100172 , 2020
Cells
Astrocytes
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Velasco, S. , Kedaigle, A. , Simmons, S. , Nash, A. , Rocha, M. , Quadrato, G. , Paulsen, B. , Nguyen, L. , Adiconis, X. , Regev, A. , Levin, J. and Arlotta, P.
Individual Brain Organoids Reproducibly Form Cell Diversity of the Human Cerebral Cortex. ,
Nature
 570 , 523-527 , 2019
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
PBS
Pancreas
Guinea-Pig
Guinea-pig, Hartley, albino, male, 350-400 g
Gardner, J. , Conlon, T. , Klaeveman, H. , Adams, T. and Ondetti, M.
Action of Cholecystokinin and Cholinergic Agents on Calcium Transport in Isolated Pancreatic Acinar Cells ,
J Clin Invest
 56 , 366 , 1975
Cells
Acinar
Enzymes
Medium
Kreb's Ringer
Pancreas
Human
Human
Cane, M. , Sutton, R. and Criddle, D.
Isolation of Human Pancreatic Acinar Cells From Rat and Human Pancreas ,
Pancreapedia
 20 , 1 , 2011
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
See Reference
Pancreas
Mouse
Mouse, adult, male
Ji, B. , Gaiser, S. , Chen, X. , Ernst, S. and Logsdon, C.
Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation. ,
J Biol Chem
 284 , 17488 , 2009
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Mouse
Mouse, 6-10 month
Wang, Y. , Soyombo, A. , Shcheynikov, N. , Zeng, W. , Dorwart, M. , Marino, C. , Thomas, P. and Muallem, S.
Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis. ,
EMBO J
 25 , 5049 , 2006
Cells
Pancreatic ductal
Enzymes
CLSPA : 50 u/ml
Hyaluronidase : 400 u/ml
Medium
DMEM
Pancreas
Mouse
Mouse, male, 18-24 g
Burnham DB, Williams JA.
Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini ,
J Biol Chem
 257 , 10523-8 , 1982
Cells
Acinar
Enzymes
CLSPA : 70-90 u/ml
Medium
Krebs-Henseleit
Pancreas
Rat
Rat, SD, adult
Li, J. , Zhou, R. , Bie, B. , Huang, N. , Guo, Y. , Chen, H. , Shi, M. , Yang, J. , Zhang, J. and Li, Z.
Emodin and Baicalein Inhibit Sodium Taurocholate-Induced Vacuole Formation in Pancreatic Acinar Cells. ,
World J Gastroenterol
 24 , 35-45 , 2018
Cells
Acinar
Enzymes
Medium
Culture media
Pancreas
Rat
Rat, SD, male
Ji B, Kopin AS, Logsdon CD.
Species differences between rat and mouse CCKA receptors determine the divergent acinar cell response to the cholecystokinin analog JMV-180 ,
J Biol Chem
 275 , 19115-20 , 2000
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Rat
Rat
Blinman TA, Gukovsky I, Mouria M, Zaninovic V, Livingston E, Pandol SJ, Gukovskaya AS.
Activation of pancreatic acinar cells on isolation from tissue: cytokine upregulation via p38 MAP kinase ,
Am J Physiol Cell Physiol
 279 , C1993-2003 , 2000
Cells
Pancreatic acini
Enzymes
CLSPA : 30 u/ml
Collagenase Type 4 : 30 u/ml
Medium
M199
Pancreas
Rat
Rat, SD, male, 150 - 200 g
Menozzi, D. , Sato, S. , Jensen, R. and Gardner, J.
Cyclic GMP Does Not Inhibit Protein Kinase C-Mediated Enzyme Secretion in Rat Pancreatic Acini ,
J Biol Chem
 264 , 995 , 1989
Cells
Acinar
Enzymes
Medium
HEPES
Pituitary
Mouse
Mouse
Pyczek, J. , Buslei, R. , Schult, D. , Holsken, A. , Buchfelder, M. , Hess, I. , Hahn, H. and Uhmann, A.
Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland. ,
Sci Rep
 6 , 24928 , 2016
Cells
Pituitary
Enzymes
Medium
DMEM
Skin
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Spleen
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Stem
Human
Human
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
Stem
Human
Human
Hjelmeland, A. , Wu, Q. , Wickman, S. , Eyler, C. , Heddleston, J. , Shi, Q. , Lathia, J. , Macswords, J. , Lee, J. , McLendon, R. and Rich, J.
Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth. ,
PLoS Biol
 8 , e1000319 , 2010
Cells
Glioma stem cells
Enzymes
PDS kit : per instructions
Medium
neurobasal medium
Stem
Mouse
Mouse
Klein C, Butt SJ, Machold RP, Johnson JE. and Fishell G.
Cerebellum- and forebrain-derived stem cells possess intrinsic regional character ,
Development
 132 , 4497 , 2005
Cells
Neurosperes
Enzymes
PDS kit : per instructions
Medium
DMEM/F12
Stem
Mouse
Mouse, 1 day
Seaberg, R. , Smukler, S. and Van der Kooy, D.
Intrinsic Differences Distinguish Transiently Neurogenic Progenitors from Neural Stem Cells in the Early Postnatal Brain. ,
Dev Biol
 278 , 71 , 2005
Cells
Neural progenitor
Enzymes
PDS kit : per instructions
Medium
see reference
Stem
Mouse
Mouse, embryonic
Estivill-Torrus, G. , Pearson, H. , Van Heyningen, V. , Price, D. and Rashbass, P.
Pax6 is Required to Regulate the Cell Cycle and the Rate of Progression from Symmetrical to Asymmetrical Division in Mammalian Cortical Progenitors. ,
Development
 129 , 455 , 2002
Cells
Cortical progenitors
Enzymes
PDS kit : per instructions
Medium
Serum free medium
Stem
Rat
Rat, 6-8 week
Mothe, A. and Tator, C.
Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord. ,
J Vis Exp
 , e52732 , 2015
Cells
Spinal cord neural progenitor
Enzymes
PDS kit : with modifications
Medium
EBSS
Stem
Rat
Rat, 2 month
Mothe, A. , Kulbatski, I. , Van Bendegem, R. , Lee, L. , Kobayashi, E. , Keating, A. and Tator, C.
Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells ,
J Histochem Cytochem
 53(10) , 1215 , 2005
Cells
Spinal cord progenitor cells
Hybrid toxins
Enzymes
PDS kit : see reference
Medium
Neurobasal A
Tumor
Human
Human
Yan, K. , Wu, Q. , Yan, D. , Lee, C. , Rahim, N. , Tritschler, I. , DeVecchio, J. , Kalady, M. , Hjelmeland, A. and Rich, J.
Glioma Cancer Stem Cells Secrete Gremlin1 to Promote their Maintenance Within the Tumor Hierarchy. ,
Genes Dev
 28 , 1085-100 , 2014
Cells
Glioma cancer stem
Enzymes
PDS kit : per instructions
Medium
Neurobasal

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.