Tissue References Search Tool

Mouse

Mouse, 6-8 week

Qian, Q. , Hunter, L. , Li, M. , Marin-Padilla, M. , Prakash, Y. , Somlo, S. , Harris, P. , Torres, V. and Sieck, G.

Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells ,
Hum Mol Genet
12(15) , 1875 , 2003

Cells

Vascular smooth muscle

Enzymes

Papain : 10 u/ml

Elastase : .005%

Collagenase : 0.05%

Deoxyribonuclease I : 1000 u/ml

PDS kit : with modifications

Medium

EBSS

Organoids

Human

Tresenrider, A. , Sridhar, A. , Eldred, K. , Cuschieri, S. , Hoffer, D. , Trapnell, C. and Reh, T.

Single-cell Sequencing of Individual Retinal Organoids Reveals Determinants of Cell Fate Heterogeneity. , bioRxiv , 2023

Cells

Retinal Organoids

Enzymes

PDS kit : see reference

Medium

DMEM

Organoids

Human

Schröter, M. , Wang, C. , Terrigno, M. , Hornauer, P. , Huang, Z. , Jagasia, R. and Hierlemann, A.

Functional Imaging of Brain Organoids Using High-Density Microelectrode Arrays. , MRS Bull Vol. 47 , 530-544 , 2022

Cells

Cerebral organoids

Enzymes

PDS kit : see reference

Medium

PBS

Organoids

Human

Uzquiano, A. , Kedaigle, A. , Pigoni, M. , Paulsen, B. , Adiconis, X. , Kim, K. , Faits, T. , Nagaraja, S. , Antón-Bolaños, N. , Gerhardinger, C. , Tucewicz, A. , Murray, E. , Jin, X. , Buenrostro, J. , Chen, F. , Velasco, S. , Regev, A. , Levin, J. and Arlotta, P.

Proper Acquisition of Cell Class Identity in Organoids Allows Definition of Fate Specification Programs of the Human Cerebral Cortex ,
Cell
185 , 3770-3788.e27 , 2022

Cells

Brain organoids

Enzymes

PDS kit : see reference

Medium

EBSS

Organoids

Human

Xue, Y. , Seiler, M. , Tang, W. , Wang, J. , Delgado, J. , McLelland, B. , Nistor, G. , Keirstead, H. and Browne, A.

Retinal Organoids On-a-Chip: a Micro-Millifluidic Bioreactor for Long-term Organoid Maintenance. ,
Lab Chip
21 , 3361-3377 , 2021

Cells

Stem cell-derived retinal organoids

Enzymes

PDS kit : see reference

Medium

EBSS

Organoids

Human

Barbar, L. , Rusielewicz, T. , Zimmer, M. , Kalpana, K. and Fossati, V.

Isolation of Human CD49f Astrocytes and iPSC-Based Neurotoxicity Assays. , STAR Protoc Vol. 1 , 100172 , 2020

Cells

Astrocytes

Enzymes

PDS kit : see reference

Medium

EBSS

Organoids

Human

Velasco, S. , Kedaigle, A. , Simmons, S. , Nash, A. , Rocha, M. , Quadrato, G. , Paulsen, B. , Nguyen, L. , Adiconis, X. , Regev, A. , Levin, J. and Arlotta, P.

Individual Brain Organoids Reproducibly Form Cell Diversity of the Human Cerebral Cortex. ,
Nature
570 , 523-527 , 2019

Cells

Brain organoids

Enzymes

PDS kit : see reference

Medium

PBS