Tissue References Search Tool

Guinea-Pig

Guinea pig, 2-4 wk old, male, female

Firth, T. , Mawe, G. and Nelson, M.

Pharmacology and Modulation of KATP Channels by Protein Kinase C and Phosphatates in Gallbladder Smooth Muscle ,
Am J Physiol Cell Physiol
278 , C1031 , 2000

Cells

Smooth muscle

Gallbladder

Enzymes

Papain : 0.1%

Medium

Krebs solution

Muscle

Guinea-Pig

Guinea-pig, adult, 250-350 g

Jennings, L. , Xu, Q. , Firth, T. , Nelson, M. and Mawe, G.

Cholesterol Inhibits Spontaneous Action Potentials and Calcium Currents in Guinea Pig Gallbladder Smooth Muscle ,
Am J Physiol
277 , G1017 , 1999

Cells

Smooth muscle

Gallbladder

Enzymes

Papain : 0.1%

Medium

NaCl, sodium glutamate, MgCl, KCl, glucose, Kreb's, and HEPES

Muscle

Human

Malysz, J. , Rovner, E. , Wake, R. and Petkov, G.

Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents. ,
J Vis Exp
, 2020

Cells

Bladder smooth muscle

Enzymes

Collagenase Type 2 : 0.1-0.2%

Papain : 0.1-0.2%

Medium

Digestion solution (see reference)

Muscle

Ovine

Sheep, adult and neonatal

Driska S. , Laudadio R. , Wolfson M. and Shaffer T.

A Method for Isolating Adult and Neonatal Airway Smooth Muscle Cells and Measuring Shortening Velocity ,
J Appl Physiol
86(1) , 427 , 1999

Cells

Tracheal smooth muscle cells

Enzymes

Papain : 0.2%

Deoxyribonuclease I : 0.1%

Medium

MOPS-PSS

Muscle

Rat

Rat, SD, 250 g

Jaggar JH.

Intravascular pressure regulates local and global Ca(2+) signaling in cerebral artery smooth muscle cells ,
Am J Physiol Cell Physiol
281 , C439-48 , 2001

Cells

Arterial smooth muscle

Enzymes

Papain : 0.03%

Collagenase : 0.1%

Medium

see reference

Muscle

Rat

Rat, SHRs and WKY, male, 10 - 14 weeks

Bolzon, B. and Cheung, D.

Isolation and Characterization of Single Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats ,
Hypertension
14 , 137 , 1989

Cells

Smooth muscle, tail arteries

Enzymes

Papain : 0.1%

Medium

HEPES buffer

(see reference)

Pancreas

Mouse

Githens, S.

Pancreatic Duct Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:12.1 , 1995

Cells

Duct

Enzymes

Papain : 25 u/ml

Medium

DMEM /F-12

Pancreas

Rat

Githens, S.

Pancreatic Duct Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:12.1 , 1995

Cells

Duct

Enzymes

Papain : 25 u/ml

Medium

DMEM /F-12

Pancreas

Rat

Rat, SD, both sexes, 6-20 wks old

Githens, S. , Schexnayder, J. , Desai, K. and Patke, C.

Rat Pancreatic Interlobular Duct Epithelium: Isolation and Culture in Collagen Gel ,
In Vitro Cell Dev Biol
25 (8) , 679 , 1989

Cells