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Tissue References Search Tool

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Muscle
Guinea-Pig
Guinea pig, 2-4 wk old, male, female
Firth, T. , Mawe, G. and Nelson, M.
Pharmacology and Modulation of KATP Channels by Protein Kinase C and Phosphatates in Gallbladder Smooth Muscle ,
Am J Physiol Cell Physiol
 278 , C1031 , 2000
Cells
Smooth muscle
Gallbladder
Enzymes
Papain : 0.1%
Medium
Krebs solution
Muscle
Guinea-Pig
Guinea-pig, adult, 250-350 g
Jennings, L. , Xu, Q. , Firth, T. , Nelson, M. and Mawe, G.
Cholesterol Inhibits Spontaneous Action Potentials and Calcium Currents in Guinea Pig Gallbladder Smooth Muscle ,
Am J Physiol
 277 , G1017 , 1999
Cells
Smooth muscle
Gallbladder
Enzymes
Papain : 0.1%
Medium
NaCl, sodium glutamate, MgCl, KCl, glucose, Kreb's, and HEPES
Muscle
Human
Human
Malysz, J. , Rovner, E. , Wake, R. and Petkov, G.
Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents. ,
J Vis Exp
 , 2020
Cells
Bladder smooth muscle
Enzymes
Collagenase Type 2 : 0.1-0.2%
Papain : 0.1-0.2%
Medium
Digestion solution (see reference)
Muscle
Ovine
Sheep, adult and neonatal
Driska S. , Laudadio R. , Wolfson M. and Shaffer T.
A Method for Isolating Adult and Neonatal Airway Smooth Muscle Cells and Measuring Shortening Velocity ,
J Appl Physiol
 86(1) , 427 , 1999
Cells
Tracheal smooth muscle cells
Enzymes
Papain : 0.2%
Medium
MOPS-PSS
Muscle
Rat
Rat, SD, 250 g
Jaggar JH.
Intravascular pressure regulates local and global Ca(2+) signaling in cerebral artery smooth muscle cells ,
Am J Physiol Cell Physiol
 281 , C439-48 , 2001
Cells
Arterial smooth muscle
Enzymes
Papain : 0.03%
Collagenase : 0.1%
Medium
see reference
Muscle
Rat
Rat, SHRs and WKY, male, 10 - 14 weeks
Bolzon, B. and Cheung, D.
Isolation and Characterization of Single Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats ,
Hypertension
 14 , 137 , 1989
Cells
Smooth muscle, tail arteries
Enzymes
Papain : 0.1%
Medium
HEPES buffer
(see reference)
Organoids
Human

Human

Geest, A. , Jakobs, C. , Ljubikj, T. , Huffels, C. , Luna, M. , Sá, R. , Adolfs, Y. , Wit, M. , Rutten, D. , Kaal, M. , Zwartkruis, M. , Carcolé, M. , Groen, E. , Hol, E. , Basak, O. , Isaacs, A. , Westeneng, H. , Berg, L. , Veldink, J. , Schlegel, D. and Pasterkamp, R.
Molecular Pathology, Developmental Changes, and Synaptic Dysfunction in (pre-) Symptomatic Human C9ORF72-ALS/FTD Cerebral Organoids. , Acta Neuropathol Commun Vol. 12 , 152 , 2024
Cells
Cerebral organoids
Enzymes
Papain : 20 u/ml
Medium
DMEM/F12
Organoids
Human

Human

Lisowski, P. , Lickfett, S. , Rybak-Wolf, A. , Menacho, C. and Le, S.
Mutant Huntingtin Impairs Neurodevelopment in Human Brain Organoids through CHCHD2-mediated Neurometabolic Failure. ,
Nat Commun
 15 , 7027 , 2024
Cells
Brain organoids
Enzymes
Papain : 20 u/ml
Medium
EBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.