For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Epithelial
Mouse
Mouse, male, 8-16 week
Breggia, A. and Himmelfarb, J.
Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. ,
Oxid Med Cell Longev
 1 , 33 , 2008
Cells
Renal tubular epithelial
Enzymes
Collagenase : 200 u/ml
Soybean Trypsin Inhibitor : see reference
Medium
HBSS
Epithelial
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Heart
Mouse
Mouse, male, 12-18 week
Jian, Z, Chen, Y, Shimkunas, R, Jian, Y, Jaradeh, M, Chavez, K, Chiamvimonvat, N, Tardiff, J, Izu, L, Ross, R. and Chen-Izu, Y
In Vivo Cannulation Methods for Cardiomyocytes Isolation from Heart Disease Models. ,
PLoS ONE
 11 , e0160605 , 2016
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 300 u/ml
Protease XIV : 0.004%
Medium
Perfusion solution (see reference)
Heart
Mouse

Mouse, 8-12 week

Ackers-Johnson, M. , Li, P. , Holmes, A. , O'Brien, S. , Pavlovic, D. and Foo, R.
A Simplified, Langendorff-Free Method for Concomitant Isolation of Viable Cardiac Myocytes and Nonmyocytes From the Adult Mouse Heart. ,
Circ Res
 119 , 909-20 , 2016
Cells
Cardiomyocytes
Enzymes
Protease XIV : 0.005%
Medium
Perfusion buffer (see reference)
Heart
Mouse
Mouse, adult, 9-13 week
Kabaeva, Z. , Zhao, M. and Michele, D.
Blebbistatin Extends Culture Life of Adult Mouse Cardiac Myocytes and Allows Efficient and Stable Transgene Expression. ,
Am J Physiol Heart Circ Physiol
 294 , H1667-74 , 2008
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 620 u/ml
Protease XIV : 0.104 u/ml
Medium
Myocyte buffer see reference
Heart
Mouse
Mouse, adult, 6-12 week
Shioya, T.
A Simple Technique for Isolating Healthy Heart Cells from Mouse Models. ,
J Physiol Sci
 57 , 327-35 , 2007
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.006%
Protease XIV : 0.006%
Medium
see reference
Heart
Mouse
Mouse, 2-4 mo
Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP.
Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology ,
Am J Physiol Heart Circ Physiol
 279 , H429-36 , 2000
Cells
Ventricular myocytes
Enzymes
Protease XIV : 0.002%
Medium
MEM
Heart
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Kidney
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Kidney
Mouse
Mouse, male, 8-16 week
Breggia, A. and Himmelfarb, J.
Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. ,
Oxid Med Cell Longev
 1 , 33 , 2008
Cells
Renal tubular epithelial
Enzymes
Collagenase : 200 u/ml
Soybean Trypsin Inhibitor : see reference
Medium
HBSS
Kidney
Mouse
Mouse, male, 12-16 week
Cunningham, R. , Xiaofei, E. , Steplock, D. , Shenolikar, S. and Weinman, E.
Defective PTH Regulation of Sodium-Dependent Phosphate Transport in NHERF-1-/- Renal Proximal Tubule Cells and Wild-Type Cells Adapted to Low-Phosphate Media. ,
Am J Physiol Renal Physiol
 289 , F933 , 2005
Cells
Renal proximal tubule cells
Enzymes
Medium
DMEM/F-12
Kidney
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Lung
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Mammary
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse, 25-30 g
Minamoto Kanji, Pinsky DavidJ.
Recipient iNOS but not eNOS deficiency reduces luminal narrowing in tracheal allografts ,
J Exp Med
 196 , 1321-33 , 2002
Cells
Tracheal inflammatory cells
Enzymes
Medium
RPMI 1640
Neural
Mouse
Mouse, embryonic 12.5
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Pancreas
Mouse
Mouse, adult, male
Ji, B. , Gaiser, S. , Chen, X. , Ernst, S. and Logsdon, C.
Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation. ,
J Biol Chem
 284 , 17488 , 2009
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Mouse
Mouse, 6-10 month
Wang, Y. , Soyombo, A. , Shcheynikov, N. , Zeng, W. , Dorwart, M. , Marino, C. , Thomas, P. and Muallem, S.
Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis. ,
EMBO J
 25 , 5049 , 2006
Cells
Pancreatic ductal
Enzymes
CLSPA : 50 u/ml
Hyaluronidase : 400 u/ml
Medium
DMEM
Pancreas
Mouse
Mouse, male, 18-24 g
Burnham DB, Williams JA.
Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini ,
J Biol Chem
 257 , 10523-8 , 1982
Cells
Acinar
Enzymes
CLSPA : 70-90 u/ml
Medium
Krebs-Henseleit
Pituitary
Mouse
Mouse
Pyczek, J. , Buslei, R. , Schult, D. , Holsken, A. , Buchfelder, M. , Hess, I. , Hahn, H. and Uhmann, A.
Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland. ,
Sci Rep
 6 , 24928 , 2016
Cells
Pituitary
Enzymes
Medium
DMEM
Skin
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Spleen
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.