Tissue References Search Tool

Cartilage

Rabbit

Rabbit, New Zealand, white, 4-6 wk & 22-25 wk

Plaas, A. , Sandy, J. and Kimura, J.

Biosynthesis of Cartilage Proteoglycan & Link Protein Articular Chondrocytes ,
J Biol Chem
263 , 7560 , 1988

Cells

Chondrocytes

Enzymes

Protease XIV : 5 mg/g of tissue

Medium

Ham's F-12

Epithelial

Mouse

Mouse, 11 week

Mueller, S. , Clark, J. , Myers, P. and Korach, K.

Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
143 , 2357 , 2002

Cells

Epithelial

Enzymes

Collagenase Type 3 : 25 u/ml

Hyaluronidase : 0.1%

Protease XIV : 0.05%

Deoxyribonuclease I : 0.04%

Medium

DMEM/F12

Heart

Mouse

Mouse, male, 12-18 week

Jian, Z, Chen, Y, Shimkunas, R, Jian, Y, Jaradeh, M, Chavez, K, Chiamvimonvat, N, Tardiff, J, Izu, L, Ross, R. and Chen-Izu, Y

In Vivo Cannulation Methods for Cardiomyocytes Isolation from Heart Disease Models. ,
PLoS ONE
11 , e0160605 , 2016

Cells

Cardiomyocytes

Enzymes

Collagenase Type 2 : 300 u/ml

Protease XIV : 0.004%

Medium

Perfusion solution (see reference)

Heart

Mouse

Mouse, 8-12 week

Ackers-Johnson, M. , Li, P. , Holmes, A. , O'Brien, S. , Pavlovic, D. and Foo, R.

A Simplified, Langendorff-Free Method for Concomitant Isolation of Viable Cardiac Myocytes and Nonmyocytes From the Adult Mouse Heart. ,
Circ Res
119 , 909-20 , 2016

Cells

Cardiomyocytes

Enzymes

Collagenase Type 2 : 0.05%

Collagenase Type 4 : 0.05%

Protease XIV : 0.005%

Medium

Perfusion buffer (see reference)

Heart

Mouse

Mouse, adult, 9-13 week

Kabaeva, Z. , Zhao, M. and Michele, D.

Blebbistatin Extends Culture Life of Adult Mouse Cardiac Myocytes and Allows Efficient and Stable Transgene Expression. ,
Am J Physiol Heart Circ Physiol
294 , H1667-74 , 2008

Cells

Cardiomyocytes

Enzymes

Collagenase Type 2 : 620 u/ml

Protease XIV : 0.104 u/ml

Deoxyribonuclease I : 0.0015%

Medium

Myocyte buffer see reference

Heart

Mouse

Mouse, adult, 6-12 week

Shioya, T.

A Simple Technique for Isolating Healthy Heart Cells from Mouse Models. ,
J Physiol Sci
57 , 327-35 , 2007

Cells

Cardiomyocytes

Enzymes

Collagenase Type 2 : 0.1%

Trypsin : 0.006%

Protease XIV : 0.006%

Medium

see reference

Heart

Mouse

Mouse, 2-4 mo

Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP.

Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology ,
Am J Physiol Heart Circ Physiol
279 , H429-36 , 2000

Cells

Ventricular myocytes

Enzymes

Collagenase Type 2 : 0.05%

Collagenase Type 4 : 0.05%

Protease XIV : 0.002%

Medium

MEM

Heart

Rabbit

Rabbit, New Zealand, 3-3.5 kg

Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.

Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
117 , 2687-2692 , 2020

Cells

Cardiac Myocytes

Enzymes

Trypsin : 0.08%

Protease XIV : 0.005%

Collagenase Type 1 : 0.1-0.2%

Medium

HEPES

Mammary

Mouse

Mouse, 11 week

Mueller, S. , Clark, J. , Myers, P. and Korach, K.

Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
143 , 2357 , 2002

Cells

Epithelial

Enzymes

Collagenase Type 3 : 25 u/ml

Hyaluronidase : 0.1%

Protease XIV : 0.05%

Deoxyribonuclease I : 0.04%

Medium

DMEM/F12

Muscle

Rabbit

Rabbit, 0.5-1 Kg
(also guinea-pig, frog, dogfish)

Mitra, R. and Morad, M.

A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
249 , H1056 , 1985

Cells

Myocytes, heart and stomach

Enzymes

Protease XIV : 0.028%

Medium

Solution C

(see reference)

Tissue Tables (references, grouped by tissue type and species)

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Tissue References Search Tool

Tissue Tables (references, grouped by tissue type and species)