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Tissue References Search Tool

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Muscle
Bovine
Bovine
Yu, M. , McAndrew, R. , Al-Saghir, R. , Maier, K. , Medhora, M. , Roman, R. and Jacobs, E.
Nitric Oxide Contributes to 20-HETE-Induced Relaxation of Pulmonary Arteries. ,
J Appl Physiol
 93 , 1391 , 2002
Cells
Pulmonary artery endothelial and smooth muscle cells
Enzymes
Collagenase : 0.04-0.05%
Medium
RPMI-1640
Muscle
Bovine
Bovine
Absher, M. , Woodcock-Mitchell, J. , Mitchell, J. , Baldor, L. , Low, R. and Warshaw, D.
Characterization of Vascular Smooth Muscle Cell Phenotype in Long-Term Culture ,
In Vitro Cell Dev Biol
 25 (2) , 183 , 1989
Cells
Smooth muscle
Enzymes
Trypsin : 0.25%
Medium
DMEM
Muscle
Bovine
Bovine
Davies, P. and Kerr, C.
Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts ,
Biochim Biophys Acta
 712 , 26 , 1982
Cells
Smooth muscle, fibroblasts
Enzymes
Trypsin : 0.055%
Medium
DMEM
Muscle
Chicken
Chick
Chamley-Campbell, J. , Campbell, G. and Ross, R.
The Smooth Muscle in Cell Culture ,
Physiol Res
 59 , 1 , 1979
Cells
Smooth muscle
Enzymes
Trypsin : 0.05% - 0.1%
Medium
HBSS
Muscle
Chicken
Chick, white leghorn, 12 day
Bullaro, J. and Brookman, D.
Comparison of Skeletal Muscle Monolayer Cultures Initiated With Cells Dissociated by the Vortex and Trypsin Methods ,
In Vitro
 12 , 564 , 1976
Cells
Muscle
Enzymes
Trypsin : 0.25%
Medium
Puck's saline A
Muscle
Chicken
Chick, white leghorn, embryos, 11 day
Tepperman, K. , Morris, G. , Essien, F. and Heywood, S.M.
A Mechanical Dissociation Method For Preparation of Muscle Cell Cultures ,
J Cell Physiol
 86 , 561 , 1975
Cells
Muscle
Enzymes
Trypsin : 0.05%
Medium
Saline G
Muscle
Chicken
Chick, embryonic
Hilfer, S.
Collagenase Treatment of Chick Heart and Thyroid , Tissue Cult Methods & Applications , Kruse, P., and Patterson, M. , 246 , 1971
Cells
Muscle
Enzymes
Trypsin : 0.1%
Medium
CMF HBSS
Muscle
Chicken
Chick embryonic
Rinaldini, L.
An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells ,
Exp Cell Res
 16 , 477 , 1959
Cells
Various tissues (heart, liver, skeletal, cardiac)
Enzymes
Trypsin : various grades
Medium
CMF Tyrode's solution
Muscle
Frog
Frog
Shepherd, N. and Kavaler, F.
Direct Control of Contraction Force of Single Frog Atrial Cells by Extracellular Ions ,
Am J Physiol
 251 , C653 , 1986
Cells
Myocytes
Enzymes
Trypsin : 0.1%
Medium
CF Ringer
Muscle
Frog
Frog, Xenopus laevis
Anderson, M.J. , Cohen, M.W. and Zorychta, E.
Effects of Innervation on the Distribution of Acetylcholine Receptors on Cultured Muscle Cells ,
J Physiol
 268 , 731 , 1977
Cells
Muscle
Enzymes
Trypsin : 0.5%
Medium
L15 medium
(see reference)
Muscle
Guinea-Pig
Guinea-pig
(also rat, rabbit)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Guinea-Pig
Guinea-pig, prepubertal
Ross, R.
The Smooth Muscle Cell . II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers ,
J Cell Biol
 50 , 172 , 1971
Cells
Smooth muscle
Aortic
Enzymes
Trypsin : 0.05%
Medium
Dulbecco-Vogt modification of Eagle's
Muscle
Hamster
Hamster, male, 60-70 day
Nakamura, T. , Iwata, Y. , Sampaolesi, M. , Hanada, H. , Saito, N. , Artman, M. , Coetzee, W. and Shigekawa, M.
Stretch-Activated Cation Channels in Skeletal Muscle Myotubes From Sarcoglycan-Deficient Hamsters ,
Am J Physiol Cell Physiol
 281 , C690 , 2001
Cells
Satellite
Enzymes
Trypsin : 0.25%
Medium
DMEM
Muscle
Human
Human
Garcia, S. , Tamaki, S. , Lee, S. , Wong, A. , Jose, A. , Dreux, J. , Kouklis, G. , Sbitany, H. , Seth, R. , Knott, P. , Heaton, C. , Ryan, W. , Kim, E. , Hansen, S. , Hoffman, W. and Pomerantz, J.
High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells. ,
Stem Cell Reports
 10 , 1160-1174 , 2018
Cells
Muscle satellite
Enzymes
Collagenase : 0.1%
Trypsin : 0.25%
Medium
DMEM
Muscle
Human
Human, fetal
(also bovine)
Davies, P. and Kerr, C.
Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts ,
Biochim Biophys Acta
 712 , 26 , 1982
Cells
Smooth muscle, fibroblasts
Enzymes
Trypsin : 0.055%
Medium
DMEM
Muscle
Human
Human
Eskin, S. , Sybers, H. , Lester, J. , Navarro, L. , Gotto, A. and DeBakey, M.
Human Smooth Muscle Cells Cultured From Atherosclerotic Plaques and Uninvolved Vessel Wall ,
In Vitro
 17 (8) , 713 , 1981
Cells
Smooth muscle
Enzymes
Trypsin : 0.25%
Medium
DMEM
Muscle
Human
Human
(also rat, guinea-pig, chick, monkey)
Chamley-Campbell, J. , Campbell, G. and Ross, R.
The Smooth Muscle in Cell Culture ,
Physiol Res
 59 , 1 , 1979
Cells
Smooth muscle
Enzymes
Trypsin : 0.05% - 0.1%
Medium
HBSS
Muscle
Monkey
Monkey (Macaca nemestrina)
Chait, A. , Ross, R. , Albers, J. and Bierman, E.
Platelet-Derived Growth Factor Stimulates Activity of LDL Receptors ,
Proc Natl Acad Sci U S A
 77 , 4084 , 1980
Cells
Smooth muscle
Enzymes
Trypsin : 0.05%
Medium
Dulbecco-Vogt
Muscle
Mouse
Mouse, 6-8 week
McKinney-Freeman SL, Jackson KA, Camargo FD, Ferrari G, Mavilio F, Goodell MA.
Muscle-derived hematopoietic stem cells are hematopoietic in origin ,
Proc Natl Acad Sci U S A
 99 , 1341 , 2002
Cells
Myocytes
Enzymes
Trypsin : 0.25%
Medium
HBSS
Muscle
Porcine
Porcine, postnatal
Miersch, C. , Stange, K. and Rontgen, M.
Effects of Trypsinization and of a Combined Trypsin, Collagenase, and DNase Digestion on Liberation and in Vitro Function of Satellite Cells Isolated from Juvenile Porcine Muscles. ,
In Vitro Cell Dev Biol Anim
 54 , 406-412 , 2018
Cells
Muscle satellite
Enzymes
Medium
Eagle MEM
Muscle
Porcine
Porcine, male, 25-40 kg
Korzick, D. , Laughlin, M. and Bowles, D.
Alterations in PKC signaling underlie enhanced myogenic tone in exercise-trained porcine coronary resistance arteries ,
J Appl Physiol
 96 , 1425-32 , 2004
Cells
Coronary myocytes
Enzymes
Medium
low calcium physiological saline
Muscle
Porcine
Porcine, adult, 35-45 kg
Wamhoff BR, Dixon JL, Sturek M.
Atorvastatin treatment prevents alterations in coronary smooth muscle nuclear Ca2+ signaling in diabetic dyslipidemia ,
J Vasc Res
 39 , 208 , 2002
Cells
Arterial smooth muscle
Enzymes
Collagenase Type 2 : 294 u/ml
Elastase : 6.5 u/ml
Deoxyribonuclease I : 0.4 mg/ml
Medium
MEM
Muscle
Porcine
Porcine
Breton, M. , Berrou, E. , Deudon, E. and Picard, J.
Changes in Proteoglycans of Cultured Pig Aortic Smooth Muscle Cells During Subculture ,
In Vitro Cell Dev Biol
 26 , 157 , 1990
Cells
Smooth muscle
Aorta
Enzymes
Trypsin : 0.05%
Medium
EDTA 0.02%
Muscle
Rabbit
Rabbit, New Zealand
Al-Shboul, O. , Mahavadi, S. , Sriwai, W. , Grider, J. and Murthy, K.
Differential Expression of Multidrug Resistance Protein 5 and Phosphodiesterase 5 and Regulation of cGMP Levels in Phasic and Tonic Smooth Muscle. ,
Am J Physiol Gastrointest Liver Physiol
 305 , G314-24 , 2013
Cells
Gastric smooth muscle
Enzymes
Medium
DMEM
Muscle
Rabbit
Rabbit, New Zealand, male, 2.5-3.5 Kg
Santos, M. , Nguyen, B.. and Thompson, J.
Factors Affecting in Vitro Growth of Harvested Enterocytes ,
Cell Transplant
 1 , 299 , 1992
Cells
Enterocytes
Enzymes
Trypsin : 0.1%
Medium
RPMI 1640 w/ 1% fetal bovine serum
PBS
Muscle
Rabbit
Rabbit
(also rat, guinea-pig)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Rabbit
Rabbit, New Zealand white, 1500 g
Knodle, S. , Anderson, S. and Papaioannou, S.
Large Scale Preparation of Rabbit Aortic Smooth Muscle Cells For Use in Calcium Uptake Studies ,
In Vitro Cell Dev Biol
 22 , 23 , 1986
Cells
Smooth muscle, aortic
Enzymes
Trypsin : 0.038%
Medium
MEM
Muscle
Rabbit
Rabbit, adult, 1-2 Kg
Benham, C.D. and Bolton, T.B.
Spontaneous Transient Outward Currents in Single Visceral and Vascular Smooth Muscle Cells of the Rabbit ,
J Physiol
 381 , 385 , 1986
Cells
Smooth muscle, ear artery
Enzymes
Trypsin : 0.1%
Medium
CF solution
(see reference)
Muscle
Rabbit
Rabbit, white New Zealand, adult, male, 2 Kg
Ives, H. , Schultz, G. , Galardy, R. and Jamieson, J.
Preparation of Functional Smooth Muscle Cells from the Rabbit Aorta ,
J Exp Med
 148 , 1400 , 1978
Cells
Smooth muscle, aorta
Enzymes
Trypsin : 0.1%
Medium
Krebs Ringer HEPES solution
Muscle
Rat
Rat
Weber, S. , Gratopp, A. , Akanbi, S. , Rheinlaender, C. , Sallmon, H. , Barikbin, P. and Koehne, P.
Isolation and Culture of Fibroblasts, Vascular Smooth Muscle, and Endothelial Cells From the Fetal Rat Ductus Arteriosus ,
Pediatr Res
 70 , 236 , 2011
Cells
Vascular smooth muscle
Enzymes
Medium
DMEM
Muscle
Rat
Rat, fetal 17-18 day
Das, M. , Rumsey, J. , Bhargava, N. , Stancescu, M. and Hickman, J.
Skeletal Muscle Tissue Engineering: A Maturation Model Promoting Long-Term Survival of Myotubes, Structural Development of the Excitation-Contraction Coupling Apparatus and Neonatal Myosin Heavy Chain Expression. ,
Biomaterials
 30 , 5392 , 2009
Cells
Myotubes
Enzymes
Trypsin : 0.05%
Medium
see reference
Muscle
Rat
Rat, SD, 3 month
Stevenson S. , Rollence M. , Marshall-Neff J. and Liau G.
A Genetically Modified Adenoviral Vector Exhibits Enhanced Gene Transfer of Human Smooth Muscle Cells ,
J Vasc Res
 38(5) , 471 , 2001
Cells
Smooth muscle cells
Enzymes
Medium
M-199
Muscle
Rat
Rat, SD, male, 150-175 g
Redmond, E. , Cahill, P. and Sitzmann, J.
Perfused Transcapillary Smooth Muscle and Endothelial Cell Co-Culture-A Novel In Vitro Model ,
In Vitro Cell Dev Biol Anim
 31 , 601 , 1995
Cells
Smooth muscle, endothelial
Enzymes
Trypsin : 0.04%
Medium
MEM
Muscle
Rat
Rat, Wistar Kyoto, 10-15 weeks
McGuire, P. , Walker-Caprioglio, H. , Little, S. and McGuffee, L.
Isolation and Culture of Rat Superior Mesenteric Artery Smooth Muscle Cells ,
In Vitro Cell Dev Biol
 29 , 135 , 1993
Cells
Smooth muscle, mesenteric artery
Enzymes
Trypsin : 0.05%
Medium
MEM
Muscle
Rat
Rat
(also rabbit, guinea-pig)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Rat
Rat, SD, 19 days
Loch-Caruso, R. , Pahl, M. and Juberg, D.
Rat Myometrial Smooth Muscle Cells Show High Levels of Gap Junctional Communication Under a Variety of Culture Conditions ,
In Vitro Cell Dev Biol
 28 , 97 , 1992
Cells
Smooth muscle, myometrial
Enzymes
Trypsin : 150 µg/ml
Medium
HBSS or PSS, CMF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.