Tissue References Search Tool

Rat

Rat, SD, 19-20 days pregnant

Ahmed, Z. , Walker, P. and Fellows, R.

Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture ,
J Neurosci
3 (12) , 2448 , 1983

Cells

Neural

Enzymes

Trypsin : 0.25%

Medium

DMEM

Rat

Rat, fetus

Swaiman, K. , Neale, E. , Fitzgerald, S. and Nelson, P.

A Method for Large-scale Production of Mouse Brain Cortical Cultures ,
Brain Res
255 , 361 , 1982

Cells

Cortical

Enzymes

Trypsin : 0.25%

Medium

Puck's D1 (see reference)

Rat

Rat, Wistar, pregnant

Abney, E. , Bartlett, P. and Raff, M.

Astrocytes, Ependymal Cells, and Oligodendrocytes Develop on Schedule in Dissociated Cell Cultures of Embryonic Rat Brain ,
Dev Biol
83 , 301 , 1981

Cells

Glial

Enzymes

Trypsin : 0.05%

Medium

Eagle's MEM/DMEM

Rat

Rat, Wistar, adult, 170 g

Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.

Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
129 , 261 , 1979

Cells

Endothelial

Enzymes

Trypsin : 0.5%

Medium

BSS

Rat

Rat, Holtzmann, 18 day

Banker, G. and Cowan, M.

Rat Hippocampal Neurons in Dispersed Cell Culture ,
Brain Res
126 , 397 , 1977

Cells

Hippocampal neurons

Enzymes

Trypsin : 0.1%

Medium

HBSS

Rat

Rat, SD, 2 days

Rowe, V. , Neale, E. , Avins, L. , Guroff, G. and Schrier, B.

Pineal gland cells in culture. Morphology, Biochemistry, Differentiation, and co-culture with sympathetic neurons ,
Exp Cell Res
104 , 345 , 1977

Cells

Pineal

Enzymes

Trypsin : 0.25%

Medium

DMEM, MEM

Shellfish

Helisoma trivolvis

Hadley, R.D. , Bodnar, D.A. and Kater, S.B.

Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation ,
J Neurosci
5 , 3145 , 1985

Cells

Neurons

Buccal ganglia

Enzymes

Trypsin : 0.2%

Medium

Saline, Sterile

Bovine

Steers, 1-2 years

Mackintosh, D. and Mason, R.

Pharmacological Actions of 17 Beta-oestradiol on Articular Cartilage Chondrocytes and Chondrosarcoma Chondrocytes in the Absence of Oestrogen Receptors ,
Biochim Biophys Acta
964 , 295 , 1988

Cells

Chondrocytes

Enzymes

Trypsin : 0.20%

Medium

HBSS

Canine

Lee, J. , Kim, H. , Kim, J. , Bae, S. , Joo, D. , Huh, K. , Fang, Y. , Jeong, J. , Kim, M. and Kim, Y.

Microencapsulation of Pancreatic Islets with Canine Ear Cartilage for Immunoisolation. ,
Transplant Proc
44 , 1091-4 , 2012

Cells

Chondrocytes

Enzymes

Collagenase Type 2 : 0.3%

Trypsin : 0.25%

Medium

Ham's F12

Chicken

Chick embryos, HH stage

Wong, M. and Tuan, R.

Nuserum, A Synthetic Serum Replacement, Supports Chondrogenesis of Embryonic Chick Limb Bud Mesenchymal Cells in Micromass Culture ,
In Vitro Cell Dev Biol
29A , 917 , 1993

Cells

Mesenchymal

Enzymes

Trypsin : 0.1%

Medium

DMEM

(see reference)

Chicken

Chicken, broiler strain, 8-10 weeks

Genge, B. , Wu, L. and Wuthier, R.

Differential Fractionation of Matrix Vesicle Proteins: Further Characterization of the Acidic Phospholipid-dependent Ca2+-Binding Proteins ,
J Biol Chem
265 , 4703 , 1990

Cells

Matrix vesicles

Epiphyseal growth plate

Enzymes

Trypsin : 0.1%

Medium

Tris-buffered saline

Chicken

Chicken, broiler strain, 8-10 week

Genge, B.R. , Wu, L.N.Y. and Wuthier, R.E.

Identification of Phospholipid-dependent Calcium-binding Proteins as Constituents of Matrix Vesicles ,
J Biol Chem
264 , 10917 , 1989

Cells

Matrix vesicles

Enzymes

Trypsin : 0.1%

Medium

(see reference)

Chicken

Chick embryos, White Leghorn, 19 day old

Gionti, E. , Capasso, O. and Cancedda, R.

The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions in Vitro ,
J Biol Chem
258 (11) , 7190 , 1983

Cells

Fibroblasts

Epithelial-like

Enzymes

Trypsin : 0.25%

Medium

E 199 medium

Chicken

Chick embryos, 19 day old

Capasso, O. , Gionti, E. , Pontarelli, G. , Ambesi-Impiobato, F. , Nitsch, L. , Tajana, G. and Cancedda, R.

The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions In Vitro ,
Exp Cell Res
142 , 197 , 1982

Cells

Chondrocytes

Enzymes

Trypsin : 0.75%

Medium

Coon's modified F-12

Chicken

Chick embryos, White Leghorn

Ahrens, P. , Solursh, M. and Reiter, R.

Stage-Related Capacity for Limb Chondrogenesis in Cell Culture ,
Dev Biol
60 , 69 , 1977

Cells

Wing buds

Enzymes

Trypsin : 0.1%

Medium

Saline G

Human

Muraglia, A. , Nguyen, V. , Nardini, M. , Mogni, M. , Coviello, D. , Dozin, B. , Strada, P. , Baldelli, I. , Formica, M. , Cancedda, R. and Mastrogiacomo, M.

Culture Medium Supplements Derived from Human Platelet and Plasma: Cell Commitment and Proliferation Support. ,
Front Bioeng Biotechnol
5 , 66 , 2017

Cells

Chondrocytes

Enzymes

Trypsin : 0.25%

Hyaluronidase : 0.1%

Collagenase Type 2 : 1000 u/ml

Collagenase Type 1 : 400 u/ml

Medium

Ham's F12

Human

Human w/ rheumatoid arthritis

Dayer, J. , Krane, S. , Russell, R. and Robinson, D.

Production of Collagenase and Prostaglandins by Isolated Adherent Rheumatoid Synovial Cells ,
Proc Natl Acad Sci U S A
73 (3) , 945 , 1976

Cells

Synovial tissue

Enzymes

Trypsin : 0.05%

Medium

DMEM

(see reference)

Human

Human, 13-62 years

Srivastava, V.M.L. , Malemud, C.J. , Hough, A.J. , Bland, J.H. and Sokoloff, L.

Preliminary Experience with Cell Culture of Human Articular Chondrocytes ,
Arthritis Rheum
17 , 165 , 1974

Cells

Articular chondrocytes

Enzymes

Trypsin : 0.2%

Medium

BSS

Mouse

Mouse, 2-5 day

Otsuru, S. , Hofmann, T. , Raman, P. , Olson, T. , Guess, A. , Dominici, M. and Horwitz, E.

Genomic and Functional Comparison of Mesenchymal Stromal Cells Prepared Using Two Isolation Methods. ,
Cytotherapy
17 , 262-70 , 2015

Cells

Chondrocytes

Enzymes

Trypsin : 0.25%

Collagenase Type 1 : 86.5 u/ml

Medium

DMEM

Rabbit

Rabbit, New Zealand, 1.8-2.3kg

Mehraban F, Tindal MH, Proffitt MM, Moskowitz RW.

Temporal pattern of cysteine endopeptidase (cathepsin B) expression in cartilage and synovium from rabbit knees with experimental osteoarthritis: gene expression in chondrocytes in response to interleukin-1 and matrix depletion ,
Ann Rheum Dis
56 , 108 , 1997

Cells

Chondrocytes

Enzymes

Hyaluronidase : .05%

Collagenase Type 2 : 0.2%

Trypsin : 0.2%

Medium

Gey's solution

Rabbit

Rabbit, white, male, 8 weeks

Benya, P.D. , Padilla, S.R. and Nimni, M.E.

The Progeny of Rabbit Articular Chondrocytes Synthesize Collagen Types I and III and Type I Trimer, but Not Type II ,
Biochemistry
16 , 865 , 1977

Cells

Chondrocytes

Enzymes

Trypsin : 0.2%

Medium

Gey's BSS

Rabbit

Rabbit, New Zealand white or Dutch, 1 week
(also human, newborn)

Schindler, F.H. , Ose, M.A. and Solursh, M.

The Synthesis of Cartilage Collagen by Rabbit and Human Chondrocytes in Primary Cell Culture ,
In Vitro
12 , 44 , 1976

Cells

Chondrocytes

Enzymes

Trypsin : 0.1%

Medium

Saline G, CMF

Rabbit

Rabbit, New Zealand white, immature, 2.25 - 3.3 Kg

Green, J.R. and William, T.

Articular Cartilage Repair. Behavior of Rabbit Chondrocytes During Tissue Culture and Subsequent Allografting ,
Clin Orthop Relat Res
, 237 , 1976

Cells

Articular chondrocytes

Hyaline

Enzymes

Trypsin : 0.2%

Medium

Gey's BSS

Rabbit

Rabbit, New Zealand white, male, 250-350 g

Bentley, G. and Greer , R.

Homotransplantation of Isolated Epiphyseal and Articular Cartilage Chondrocytes into Joint Surfaces of Rabbits ,
Nature
230 , 385 , 1971

Cells

Epiphyseal

Articular cartilage

Enzymes

Trypsin : 0.25%

Medium

Eagle's basal medium

Rat

Mei, L. , Shen, B. , Ling, P. , Liu, S. , Xue, J. , Liu, F. , Shao, H. , Chen, J. , Ma, A. and Liu, X.

Culture-expanded Allogenic Adipose Tissue-derived Stem Cells Attenuate Cartilage Degeneration in an Experimental Rat Osteoarthritis Model. ,
PLoS ONE
12 , e0176107 , 2017

Cells

Chondrocytes

Enzymes

Collagenase Type 2 : 0.15%

Trypsin : 0.25%

Medium

DMEM/F12

Rat

Rat, SD, young, 100-120 g

Shimomura, Y. , Yoneda, T. and Suzuki, F.

Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib ,
Calcif Tissue Res
19 , 179 , 1975

Cells

Chondrocytes

Enzymes

Trypsin : 0.2%

Medium

Ham's F-12 medium

Guinea-Pig

Guinea-pig, adult

Raghupathi, R. , Duffield, M. , Zelkas, L. , Meedeniya, A. , Brookes, S. , Sia, T. , Wattchow, D. , Spencer, N. and Keating, D.

Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells. ,
J Physiol
591 , 5959-75 , 2013

Cells

Enterochromaffin

Enzymes

Trypsin : 0.05%

Collagenase Type 1 : 0.1%

Medium

DMEM

Human

Ali, M. , Anand, S. , Tangella, K. , Ramkumar, D. and Saif, T.

Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry. ,
J Vis Exp
, e52532 , 2015

Cells

Colon

Enzymes

Trypsin : 0.1%

Collagenase : 0.1%

Medium

HBSS

Human

Raghupathi, R. , Duffield, M. , Zelkas, L. , Meedeniya, A. , Brookes, S. , Sia, T. , Wattchow, D. , Spencer, N. and Keating, D.

Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells. ,
J Physiol
591 , 5959-75 , 2013

Cells

Enterochromaffin

Enzymes

Trypsin : 0.05%

Collagenase Type 1 : 0.1%

Medium

DMEM

Rat

Rat, adult

Somara, S. , Bashllari, D. , Gilmont, R. and Bitar, K.

Real-Time Dynamic Movement of Caveolin-1 During Smooth Muscle Contraction of Human Colon and Aged Rat Colon Transfected with Caveolin-1 cDNA. ,
Am J Physiol Gastrointest Liver Physiol
300 , G1022 , 2011

Cells

Colon smooth muscle

Enzymes

Soybean Trypsin Inhibitor : 0.01%

Collagenase Type 2 : 0.1%

Medium

DMEM

Bovine

Yu, M. , McAndrew, R. , Al-Saghir, R. , Maier, K. , Medhora, M. , Roman, R. and Jacobs, E.

Nitric Oxide Contributes to 20-HETE-Induced Relaxation of Pulmonary Arteries. ,
J Appl Physiol
93 , 1391 , 2002

Cells

Pulmonary artery endothelial and smooth muscle cells

Enzymes

Collagenase : 0.04-0.05%

Soybean Trypsin Inhibitor : 0.04%

Medium

RPMI-1640

Bovine

Calf

Vender, R.

Role of Endothelial Cells in the Proliferative Response of Cultured Pulmonary Vascular Smooth Muscle Cells to Reduced Oxygen Tension ,
In Vitro Cell Dev Biol
28A , 403 , 1992

Cells

Endothelial

Enzymes

Trypsin : 0.25%

Medium

HEPES

Bovine

DeNucci, G. , Gryglewski, R.J. , Warner, T.D. and Vane, J.R.

Receptor-Mediated Release of Endothelium-Derived Relaxing Factor and Prostacyclin From Bovine Aortic Endothelial Cells Is Coupled ,
Proc Natl Acad Sci U S A
85 , 2334 , 1988

Cells

Endothelial

Aortic

Enzymes

Trypsin : 0.05%

Medium

Krebs Ringer solution

Bovine

Scott, D. , Murray, J. and Barnes, M.

Investigation of the Attachment of Bovine Corneal Endothelial Cells ,
Exp Cell Res
144 , 472 , 1983

Cells

Endothelial

Corneal

Enzymes

Trypsin : 0.2%

Medium

PBS: DMEM