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Tissue References Search Tool

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Neural
Rat
Rat, SD, female, 4 month old
Wood, P. and Bunge, R.
Evidence That Axons are Mitogenic for Oligodendrocytes Isolated From Adult Animals ,
Nature
 320 , 756 , 1986
Cells
CNS cells
Enzymes
Trypsin : 0.25%
Medium
EBSS
Neural
Rat
Rat, embryo, 15 day
Jahr, C. and Jessell, T.
Synaptic Transmission between Dorsal Root Ganglion and Dorsal Horn Neurons in Culture: Antagonism of Monosynaptic Excitatory Postsynaptic Potentials and Glutamate Excitation by Kynurenate ,
J Neurosci
 5 , 2281 , 1985
Cells
Dorsal horn neurons
Spinal
Enzymes
Trypsin : 0.025%
Medium
Ham's F-12
Neural
Rat
Rat, fetus, 18-20 day
Bartlett, W. and Banker, G.
An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts ,
J Neurosci
 4 , 1944 , 1984
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Neural
Rat
Rat, SD, pups
Sarthy PV, Curtis BM. and Catterall WA.
Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat ,
J Neurosci
 3 (12) , 2532 , 1983
Cells
Retina
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Neural
Rat
Rat, W/FU, 5-8 day
Raff, M. , Fields, K. , Hakomori, S. , Mirsky, R. , Pruss, R. and Winter, J.
Cell-Type-Specific Markers for Distinguishing and Studying Neurons And the Major Classes of Glial Cells in Culture ,
Brain Res
 174 , 283 , 1979
Cells
Neurons and glial
Enzymes
Trypsin : 0.25%
Medium
MEM
(see reference)
Neural
Rat
Rat, fetus
Wakshull, E. , Johnson, M. and Burton, H.
Postnatal Rat Sympathetic Neurons In Culture. 1. A Comparison With Embryonic Neurons ,
J Neurophysiol
 42 , 1410 , 1979
Cells
Neurons, sympathetic
Enzymes
Trypsin : 0.25%
Medium
L-15 or HBSS, CMF
Neural
Rat
Rat, Wistar/Furth, newborn
(also bovine)
Brockes, J. , Fields, K. and Raff, M.
Studies on Cultured Rat Schwann Cells. I. Establishment of Purified Populations From Cultures of Peripheral Nerve ,
Brain Res
 165 , 105 , 1979
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, embryo
Dichter, M.
Rat Cortical Neurons in Cell Culture: Culture Methods, Cell Morphology, Electrophysiology, and Synapse Formation ,
Brain Res
 149 , 279 , 1978
Cells
Neurons, cortical
Enzymes
Trypsin : 0.027%
Medium
MEM
Neural
Rat
Rat, neonatal
Mains, R. and Patterson, P.
Primary Cultures of Dissociated Sympathetic Neurons I. Establishment of Long-Term Growth in Culture and Studies of Differentiated Properties ,
J Cell Biol
 59 , 329 , 1973
Cells
Neurons, superior cervical ganglia
Enzymes
Trypsin : 0.1%
Medium
Basal L-15 medium
Pancreas
Rat
Rat, SD, male, 100 - 150 g
Yeh, C. , Mertz, P. , Oliver, C. , Baum, B. and Kousvelari, E.
Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells ,
In Vitro Cell Dev Biol
 27 , 707 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.001%
Medium
F12 medium
Pancreas
Rat
Rat, Wistar, male
Foskett, J. , Roifman, C. and Wong, D.
Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells ,
J Biol Chem
 266 , 2778 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.02%
Medium
Solution B
(See reference)
Pancreas
Rat
Rat, Fischer-344, either sex, 120-150 g
Tsao, M. and Duguid, W.
Establishment of Propagable Epithelial Cell Lines From Normal Adult Rat Pancreas ,
Exp Cell Res
 168 , 365 , 1987
Cells
Epithelial
Enzymes
Trypsin : 0.1%
Medium
Ham's F-12/HBSS
(see reference)
Pancreas
Rat
Rat, SD, male, 50 - 125 g
Oliver, C. , Waters, J. , Tolbert, C. and Kleinman, H.
Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel ,
In Vitro Cell Dev Biol
 23 , 465 , 1987
Cells
Acinar
Exorbital lacrimal, parotid, pancreas
Enzymes
Trypsin : 0.01%
Medium
HBSS, CMF
Pancreas
Rat
Rat, SD, 125 - 350 g
Githens, S. , Holmquist, D. , Whelan, J. and Ruby, J.
Ducts of the Rat Pancreas in Agarose Matrix Culture ,
In Vitro
 16 , 797 , 1980
Cells
Duct
Enzymes
Trypsin : 0.01%
Medium
HBSS
Pancreas
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF
Pancreas
Rat
Rat, neonate
Leonard, R. , Lazarow, A. and Hegre, O.
Pancreatic Islet Transplantation in the Rat ,
Diabetes
 22 , 413 , 1973
Cells
Islets
Enzymes
Trypsin : 0.05%
Medium
Puck's saline buffered w/ EDTA 0.02%
Pancreas
Rat
Rat
Essner, E.
Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands ,
Exp Cell Res
 7 , 430 , 1954
Cells
Ascites hepatoma
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer (see reference)
Parotid
Rat
Rat, SD, male, 100-150 g
Yeh, C. , Mertz, P. , Oliver, C. , Baum, B. and Kousvelari, E.
Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells ,
In Vitro Cell Dev Biol
 27 , 707 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.001%
Medium
F12 medium
Parotid
Rat
Rat, Wistar, male
Foskett, J. , Roifman, C. and Wong, D.
Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells ,
J Biol Chem
 266 , 2778 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.02%
Medium
Solution B
(See reference)
Parotid
Rat
Rat, SD, male, 50 - 125 g
Oliver, C. , Waters, J. , Tolbert, C. and Kleinman, H.
Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel ,
In Vitro Cell Dev Biol
 23 , 465 , 1987
Cells
Acinar
Exorbital lacrimal, parotid, pancreas
Enzymes
Trypsin : 0.01%
Medium
HBSS, CMF
Parotid
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF
Pituitary
Rat
Rat, Wistar, male, 2 month old
Zhou, X. , De Schepper, J. , De Craemer, D. , Delhase, M. , Gys, G. , Smitz, J. and Hooghe-Peters, E.
Pituitary Growth Hormone Release and Gene Expression in Cafeteria-Diet-Induced Obese Rats ,
J Endocrinol
 159 , 165 , 1998
Cells
Pituitary
Enzymes
Trypsin : 0.1%
Medium
DMEM
Pituitary
Rat
Rat, SD, female, 200 - 250 g
D'Emden, M. and Wark, J.
Culture Requirements for Optimal Expression of 1,25-Dihydroxyvitamin D3-Enhanced Thyrotropin Secretion ,
In Vitro Cell Dev Biol
 27 , 197 , 1991
Cells
Anterior pituitary gland
Enzymes
Trypsin : 0.1%
Medium
EBSS, CMF
Pituitary
Rat
Rat
Wilfinger, W. , Larsen, W. , Downs, T. and Wilbur, D.
An In Vitro Model for Studies of Intercellular Communication in Cultured Rat Anterior Pituitary Cells ,
Tissue Cell
 16 (4) , 483 , 1984
Cells
Pituitary
Enzymes
Trypsin : 0.3 %
Medium
HEPES
Pituitary
Rat
Rat, SD, male, 250-450 g
Portanova, R. , Smith, D. and Sayers, G.
A Trypsin for the Preparation of Isolated Rat Anterior Pituitary ,
Proc Soc Exp Biol Med
 133 , 573 , 1970
Cells
Anterior pituitary
Enzymes
Trypsin : 0.25%
Medium
Krebs
Reproductive
Rat
Rat, SD, adult, male, 8 - 10 week
Abou-Haila, A. and Tulsiani, D.
Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization ,
Biol Proced Online
 3 (1) , 35 , 2001
Cells
Seminiferous tubules
Enzymes
Trypsin : 0.05%
Medium
Krebs-Ringer bicarbonate buffer
(see reference)
Reproductive
Rat
Rat, Han-Wistar, adult, male, 250 g
Leckie, C. , Welberg, L. and Seckl, J.
11B-Hydroxysteroid Dehydrogenase is a Predominant Reductase in Intact Rat Leydig Cells ,
J Endocrinol
 159 , 233 , 1998
Cells
Testes
Enzymes
Trypsin : 0.1%
Medium
Medium 199 w/ Hank's salts
Reproductive
Rat
Rat, SD, male
Abayasekara, D. , Kurlak, L. , Band, A. , Sullivan, M. and Cooke, B.
Effect of Cell Purity, Cell Concentration, and Incubation Conditions on Rat Testis Leydig Cell Steroidogenesis ,
In Vitro Cell Dev Biol
 27 , 253 , 1991
Cells
Leydig
Enzymes
Trypsin : 0.02%
Medium
DMEM
Reproductive
Rat
Rat, neonatal
Branham, W. , Lyn-Cook, B. , Andrews, A. , McDaniel, M. and Sheehan, D.
Growth of Neonatal Rat Uterine Luminal Epithelium on Extracellular Matrix ,
In Vitro Cell Dev Biol
 27 , 442 , 1991
Cells
Uterine
Enzymes
Trypsin : 1%
Medium
HBSS
Reproductive
Rat
Rat, Wistar, adult
Pampfer, S. , Vanderheyden, I. , Michiels, B. and DeHertogh, R.
Co-Culture of Two-Cell Rat Embryos on Cell Monolayers ,
In Vitro Cell Dev Biol
 26 , 944 , 1990
Cells
Uterine
Enzymes
Trypsin : 0.5%
Medium
PBS
Reproductive
Rat
Rat, SD, female
Glasser, S. , Julian, J. , Decker, G. , Tang, J. and Carson, D.
Development of Morphological and Functional Polarity in Primary Cultures of Immature Rat Uterine Epithelial Cells ,
J Cell Biol
 107 (6) , 2409 , 1988
Cells
Luminal epithelial
Enzymes
Trypsin : 0.5%
Medium
HBSS
Reproductive
Rat
Rat, male, 20 day
Skinner, M. , Fetterolf, P. and Anthony, C.
Purification of a Paracrine Factor, P-Mod-S,Produced by Testicular Peritubular Cells That Modulates Sertoli Cell Function ,
J Biol Chem
 263 , 2884 , 1988
Cells
Sertoli
Enzymes
Trypsin : 0.15%
Medium
(see reference)
Reproductive
Rat
Rat, Wistar, virgin, female, 250 g
Conti, C. and Tasat, D.
Regulation of Cultured Rat Vaginal Epithelial Cells By 17B-Estradiol and Progesterone ,
J Steroid Biochem
 24 (3) , 747 , 1986
Cells
Vaginal epithelial
Enzymes
Trypsin : 0.5%
Medium
PBS
Reproductive
Rat
Rat, SD, male, 10 day
Hadley, M. , Byers, S. , Suarez-Quian, C. , Kleinman, H. and Dym, M.
Extracellular Matrix Regulates Seratoli Cell Differentiation, Testicular Cord Formation and germ cell development in vitro ,
J Cell Biol
 101 , 1511 , 1985
Cells
Sertoli
Enzymes
Trypsin : 0.025%
Medium
DMEM
Skin
Rat
Rat, Wistar, 4-8 day
Sugihara, H. and Toda, S
Primary Tissue Intact and Dissociated Cell Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 3A:2.1 , 1995
Cells
Dermal fibroblasts and keratinocytes
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Skin
Rat
Rat, SD, male, 40-60 days
Laurent, S. , Mednieks, M. and Rosenfield, R.
Growth of Sebaceous Cells in Monolayer Culture ,
In Vitro Cell Dev Biol
 28 , 83 , 1992
Cells
Sebaceous
Enzymes
Trypsin : 0.2%
Medium
DMEM with FBS
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.