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Tissue References Search Tool

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Brain
Bovine
Calf
(also lamb)
Poduslo, S. , Miller, K. and McKhann, G.
Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
 253 , 1592 , 1978
Cells
Oligodendroglia
Neural
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Brain
Guinea-Pig
Guinea-pig, 200-400 g
Kay, A.R. and Wong, R.K.S.
Isolation of Neurons Suitable for Patch-Clamping from Adult Mammalian Central Nervous Systems ,
J Neurosci Methods
 16 , 227 , 1986
Cells
Neurons
Enzymes
Trypsin : 0.06-0.08%
Medium
PIPES saline
Brain
Human
Human
Mizee, M. , Miedema, S. , van der Poel, M. , Adelia , S. , van Strien, M. , Melief, J. , Smolders, J. , Hendrickx, D. , Heutinck, K. , Hamann, J. and Huitinga, I.
Isolation of Primary Microglia from the Human Post-Mortem Brain: Effects of Ante- and Post-Mortem Variables. ,
Acta Neuropathol
 5 , 16 , 2017
Cells
Microglia
Enzymes
Collagenase Type 1 : 300 u/ml
Trypsin : 0.125%
Medium
DMEM
Brain
Human
Human
Klegeris Andis, McGeer PatrickL.
Chymotrypsin-like proteases contribute to human monocytic THP-1 cell as well as human microglial neurotoxicity ,
Glia
 51 , 56-64 , 2005
Cells
Microglia
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Brain
Mouse
Mouse, postnatal
Sher, F. , Rossler, R. , Brouwer, N. , Balasubramaniyan, V. , Boddeke, E. and Copray, S.
Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2. ,
Stem Cells
 26 , 2875 , 2008
Cells
Astrocytes
Enzymes
Trypsin : 0.25%
Deoxyribonuclease I : 1,000 u/ml
Medium
HBSS
Brain
Mouse
Mouse, SD, 8-12 week
O'Donnell SL, Frederick TJ, Krady JK, Vannucci SJ, Wood TL.
IGF-I and microglia/macrophage proliferation in the ischemic mouse brain ,
Glia
 39 , 85 , 2002
Cells
Microglia
Enzymes
Medium
RPMI-1640
Brain
Mouse
Mouse
Lim, D. and Alvarez-Buylla, A.
Interaction Between Astrocytes and Adult Subventricular Zone Precursors Stimulates Neurogenesis ,
Proc Natl Acad Sci U S A
 96 , 7526 , 1999
Cells
Astrocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM
Brain
Ovine
Lamb
(also calf)
Poduslo, S. , Miller, K. and McKhann, G.
Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
 253 , 1592 , 1978
Cells
Oligodendroglia
Neural
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Brain
Rat
Rat, SD
Akanda, N. , Tofighi, R. , Brask, J. , Tamm, C. , Elinder, F. and Ceccatelli, S.
Voltage-Dependent Anion Channels (VDAC) in the Plasma Membrane Play a Critical Role in Apoptosis in Differentiated Hippocampal Neurons but not in Neural Stem Cells. ,
Cell Cycle
 7 , 3225 , 2008
Cells
Hippocampal
Enzymes
Trypsin : 0.1%
Medium
Neurobasal
Brain
Rat
Rat, Wistar, 1-3 day
Velasco Myrian, Garci­a Esperanza, Onetti Carlos G.
Glucose deprivation activates diversity of potassium channels in cultured rat hippocampal neurons ,
Cell Mol Neurobiol
 26 , 307-19 , 2006
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.05%
Medium
DMEM
Brain
Rat
Rat, newborn
Holzwarth, J. , Glaum, S. and Miller, R.
Activation of Endothelin Receptors by Sarafotoxin Regulates Ca2+ Homeostasis in Cerebellar Astrocytes ,
Glia
 5 , 239 , 1992
Cells
Astrocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM, HBSS
Brain
Rat
Rat, SD, newborn
Pixley, S.K.
The Olfactory Nerve Contains Two Populations of Glia ,
In Vitro
 5 , 269 , 1992
Cells
Glial
Enzymes
Trypsin : 0.0625%
Medium
MEM, sterile
Brain
Rat
Rat, 60-72 hours old
Acheson, A. , Barker, P. , Alderson, R. , Miller, F. and Murphy, R.
Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF ,
Neuron
 7 , 265 , 1991
Cells
Fibroblasts
Enzymes
Trypsin : 0.2%
Medium
HEPES buffered DMEM
Brain
Rat
Rat, SD, 5 day
Mattson, M. and Kater, S.
Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions ,
Brain Res
 490 , 110 , 1989
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.2%
Deoxyribonuclease I : 10 ug/ml
Medium
HBSS
Brain
Rat
Rat, albino, adult and newborn
Giulian, D. and Baker, T.J.
Characterization of Ameboid Microglia Isolated From Developing Mammalian Brain ,
J Neurosci
 6 , 2163 , 1986
Cells
Cerebral cortices
Enzymes
Trypsin : 0.25%
Medium
PBS
Brain
Rat
Rat, Wistar, newborn
Goldman, J.E. , Geier, S.S. and Hirano, M.
Differentiation of Astrocytes and Oligodendrocytes From Germinal Matrix Cells in Primary Culture ,
J Neurosci
 6 , 52 , 1986
Cells
Germinal matrix
Enzymes
Trypsin : 0.25%
Medium
HBSS
Brain
Rat
Rat, fetus, 18 day
Rothman, S.
Synaptic Release of Excitatory Amino Acid Neurotransmitter Mediates Anoxic Neuronal Death ,
J Neurosci
 4 (7) , 1884 , 1984
Cells
Hippocampi
Enzymes
Trypsin : 0.1%
Medium
HBSS
Brain
Rat
Rat, fetus, 18-20 day
Bartlett, W. and Banker, G.
An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts ,
J Neurosci
 4 , 1944 , 1984
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Brain
Rat
Rat, SD, 19-20 days pregnant
Ahmed, Z. , Walker, P. and Fellows, R.
Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture ,
J Neurosci
 3 (12) , 2448 , 1983
Cells
Neural
Enzymes
Trypsin : 0.25%
Medium
DMEM
Brain
Rat
Rat, fetus
Swaiman, K. , Neale, E. , Fitzgerald, S. and Nelson, P.
A Method for Large-scale Production of Mouse Brain Cortical Cultures ,
Brain Res
 255 , 361 , 1982
Cells
Cortical
Enzymes
Trypsin : 0.25%
Medium
Puck's D1 (see reference)
Brain
Rat
Rat, Wistar, pregnant
Abney, E. , Bartlett, P. and Raff, M.
Astrocytes, Ependymal Cells, and Oligodendrocytes Develop on Schedule in Dissociated Cell Cultures of Embryonic Rat Brain ,
Dev Biol
 83 , 301 , 1981
Cells
Glial
Enzymes
Trypsin : 0.05%
Medium
Eagle's MEM/DMEM
Brain
Rat
Rat, Wistar, adult, 170 g
Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.
Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
 129 , 261 , 1979
Cells
Endothelial
Enzymes
Trypsin : 0.5%
Medium
BSS
Brain
Rat
Rat, Holtzmann, 18 day
Banker, G. and Cowan, M.
Rat Hippocampal Neurons in Dispersed Cell Culture ,
Brain Res
 126 , 397 , 1977
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.1%
Medium
HBSS
Brain
Rat
Rat, SD, 2 days
Rowe, V. , Neale, E. , Avins, L. , Guroff, G. and Schrier, B.
Pineal gland cells in culture. Morphology, Biochemistry, Differentiation, and co-culture with sympathetic neurons ,
Exp Cell Res
 104 , 345 , 1977
Cells
Pineal
Enzymes
Trypsin : 0.25%
Medium
DMEM, MEM
Brain
Shellfish
Helisoma trivolvis
Hadley, R.D. , Bodnar, D.A. and Kater, S.B.
Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation ,
J Neurosci
 5 , 3145 , 1985
Cells
Neurons
Buccal ganglia
Enzymes
Trypsin : 0.2%
Medium
Saline, Sterile
Kidney
Fish
Winter flounder (Pseudopleur-onectes americanus)
200 - 500 g
Dickman, K. and Renfro, J.
Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport ,
Am J Physiol
 251 , 424 , 1986
Cells
Renal tubule
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
CMF solution
Kidney
Guinea-Pig
Guinea-pig, male, 100 g
Phillips, H.
Dissociation of Single Cells from Lung or Kidney Tissue with Elastase ,
In Vitro
 8 , 101 , 1972
Cells
Single cells
Enzymes
Trypsin : 0.25%
Medium
CF salt solution
Kidney
Human
Human
McAteer, J, Kempson, S. and Evan, A
Culture of Human Renal Cortex Epithelial Cells ,
J Tiss Cul Meth
 13 , 143 , 1991
Cells
Renal cortex
Enzymes
Trypsin : 0.1%
Medium
Tissue Culture Grade Water
Kidney
Human
Human
Heieren, M. , van der Woude, F. and Balfour Jr., H.
Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells ,
Proc Natl Acad Sci U S A
 85 , 1642 , 1988
Cells
Mesangial
Enzymes
Trypsin : 0.25%
Medium
DMEM/Ham's F-12
Kidney
Human
Human, 24 newborn and stillborn
(also rabbit)
De Oca, H. and Malinin, T.
Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys ,
J Clin Microbiol
 2 , 243 , 1975
Cells
Renal
Enzymes
Trypsin : 0.25%
Medium
See reference
Kidney
Monkey
Monkey
Melnick, J. and Wallis, C.
Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells ,
Dev Biol
 37 , 77 , 1976
Cells
Kidney
Enzymes
Trypsin : 0.01% - 0.00001%
Medium
Eagle's MEM
Kidney
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Kidney
Rabbit
Rabbit, New Zealand, white
Grenier, F. and Smith, W.
Formation of 6-keto-PGF-1aby Collecting Tubule Cells Isolated from Rabbit Renal Papillae ,
Prostaglandins
 16 , 759 , 1978
Cells
Collecting tubule
Enzymes
Trypsin : 0.05%
Medium
Kreb's Ringer buffer
Kidney
Rat
Rat
Wallach, D. , Anderson, W. and Pastan, I.
Activation of Adenylate Cyclase in Cultured Fibroblasts by Trypsin ,
J Biol Chem
 253 , 24 , 1978
Cells
Fibroblasts
Kidney
Enzymes
Trypsin : up to 0.25%
Medium
Dulbecco-Vogt MEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.