Tissue References Search Tool

Brain

Bovine

Calf
(also lamb)

Poduslo, S. , Miller, K. and McKhann, G.

Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
253 , 1592 , 1978

Cells

Oligodendroglia

Neural

Enzymes

Trypsin : 0.1%

Medium

(see reference)

Brain

Guinea-Pig

Guinea-pig, 200-400 g

Kay, A.R. and Wong, R.K.S.

Isolation of Neurons Suitable for Patch-Clamping from Adult Mammalian Central Nervous Systems ,
J Neurosci Methods
16 , 227 , 1986

Cells

Neurons

Enzymes

Trypsin : 0.06-0.08%

Medium

PIPES saline

Brain

Human

Mizee, M. , Miedema, S. , van der Poel, M. , Adelia , S. , van Strien, M. , Melief, J. , Smolders, J. , Hendrickx, D. , Heutinck, K. , Hamann, J. and Huitinga, I.

Isolation of Primary Microglia from the Human Post-Mortem Brain: Effects of Ante- and Post-Mortem Variables. ,
Acta Neuropathol
5 , 16 , 2017

Cells

Microglia

Enzymes

Collagenase Type 1 : 300 u/ml

Trypsin : 0.125%

Medium

DMEM

Brain

Human

Klegeris Andis, McGeer PatrickL.

Chymotrypsin-like proteases contribute to human monocytic THP-1 cell as well as human microglial neurotoxicity ,
Glia
51 , 56-64 , 2005

Cells

Microglia

Enzymes

Trypsin : 0.25%

Deoxyribonuclease I : .005%

Medium

DMEM/F12

Brain

Mouse

Mouse, postnatal

Sher, F. , Rossler, R. , Brouwer, N. , Balasubramaniyan, V. , Boddeke, E. and Copray, S.

Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2. ,
Stem Cells
26 , 2875 , 2008

Cells

Astrocytes

Enzymes

Trypsin : 0.25%

Deoxyribonuclease I : 1,000 u/ml

Medium

HBSS

Brain

Mouse

Mouse, SD, 8-12 week

O'Donnell SL, Frederick TJ, Krady JK, Vannucci SJ, Wood TL.

IGF-I and microglia/macrophage proliferation in the ischemic mouse brain ,
Glia
39 , 85 , 2002

Cells

Microglia

Enzymes

Trypsin : 0.125%

Collagenase Type 2 : 0.01%

Deoxyribonuclease I : .005%

Medium

RPMI-1640

Brain

Mouse

Lim, D. and Alvarez-Buylla, A.

Interaction Between Astrocytes and Adult Subventricular Zone Precursors Stimulates Neurogenesis ,
Proc Natl Acad Sci U S A
96 , 7526 , 1999

Cells

Astrocytes

Enzymes

Trypsin : 0.25%

Medium

DMEM

Brain

Ovine

Lamb
(also calf)

Poduslo, S. , Miller, K. and McKhann, G.

Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
253 , 1592 , 1978

Cells

Oligodendroglia

Neural

Enzymes

Trypsin : 0.1%

Medium

(see reference)

Brain

Rat

Rat, SD

Akanda, N. , Tofighi, R. , Brask, J. , Tamm, C. , Elinder, F. and Ceccatelli, S.

Voltage-Dependent Anion Channels (VDAC) in the Plasma Membrane Play a Critical Role in Apoptosis in Differentiated Hippocampal Neurons but not in Neural Stem Cells. ,
Cell Cycle
7 , 3225 , 2008

Cells

Hippocampal

Enzymes

Trypsin : 0.1%

Medium

Neurobasal

Brain

Rat

Rat, Wistar, 1-3 day

Velasco Myrian, Garcia Esperanza, Onetti Carlos G.

Glucose deprivation activates diversity of potassium channels in cultured rat hippocampal neurons ,
Cell Mol Neurobiol
26 , 307-19 , 2006

Cells

Hippocampal neurons

Enzymes

Trypsin : 0.05%

Medium

DMEM

Brain

Rat

Rat, newborn

Holzwarth, J. , Glaum, S. and Miller, R.

Activation of Endothelin Receptors by Sarafotoxin Regulates Ca2+ Homeostasis in Cerebellar Astrocytes ,
Glia
5 , 239 , 1992

Cells

Astrocytes

Enzymes

Trypsin : 0.25%

Medium

DMEM, HBSS

Brain

Rat

Rat, SD, newborn

Pixley, S.K.

The Olfactory Nerve Contains Two Populations of Glia ,
In Vitro
5 , 269 , 1992

Cells

Glial

Enzymes

Trypsin : 0.0625%

Medium

MEM, sterile

Brain

Rat

Rat, 60-72 hours old

Acheson, A. , Barker, P. , Alderson, R. , Miller, F. and Murphy, R.

Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF ,
Neuron
7 , 265 , 1991

Cells

Fibroblasts

Enzymes

Trypsin : 0.2%

Medium

HEPES buffered DMEM

Brain

Rat

Rat, SD, 5 day

Mattson, M. and Kater, S.

Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions ,
Brain Res
490 , 110 , 1989

Cells

Hippocampal neurons

Enzymes

Trypsin : 0.2%

Deoxyribonuclease I : 10 ug/ml

Medium

HBSS

Brain

Rat

Rat, albino, adult and newborn

Giulian, D. and Baker, T.J.

Characterization of Ameboid Microglia Isolated From Developing Mammalian Brain ,
J Neurosci
6 , 2163 , 1986

Cells

Cerebral cortices

Enzymes

Trypsin : 0.25%

Medium

PBS

Brain

Rat

Rat, Wistar, newborn

Goldman, J.E. , Geier, S.S. and Hirano, M.

Differentiation of Astrocytes and Oligodendrocytes From Germinal Matrix Cells in Primary Culture ,
J Neurosci
6 , 52 , 1986

Cells

Germinal matrix

Enzymes

Trypsin : 0.25%

Medium

HBSS

Brain

Rat

Rat, fetus, 18 day

Rothman, S.

Synaptic Release of Excitatory Amino Acid Neurotransmitter Mediates Anoxic Neuronal Death ,
J Neurosci
4 (7) , 1884 , 1984

Cells

Hippocampi

Enzymes

Trypsin : 0.1%

Medium

HBSS

Brain

Rat

Rat, fetus, 18-20 day

Bartlett, W. and Banker, G.

An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts ,
J Neurosci
4 , 1944 , 1984

Cells

Hippocampal neurons

Enzymes

Trypsin : 0.25%

Medium

HBSS, CMF

Brain

Rat

Rat, SD, 19-20 days pregnant

Ahmed, Z. , Walker, P. and Fellows, R.

Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture ,
J Neurosci
3 (12) , 2448 , 1983

Cells

Neural

Enzymes

Trypsin : 0.25%

Medium

DMEM

Brain

Rat

Rat, fetus

Swaiman, K. , Neale, E. , Fitzgerald, S. and Nelson, P.

A Method for Large-scale Production of Mouse Brain Cortical Cultures ,
Brain Res
255 , 361 , 1982

Cells

Cortical

Enzymes

Trypsin : 0.25%

Medium

Puck's D1 (see reference)

Brain

Rat

Rat, Wistar, pregnant

Abney, E. , Bartlett, P. and Raff, M.

Astrocytes, Ependymal Cells, and Oligodendrocytes Develop on Schedule in Dissociated Cell Cultures of Embryonic Rat Brain ,
Dev Biol
83 , 301 , 1981

Cells

Glial

Enzymes

Trypsin : 0.05%

Medium

Eagle's MEM/DMEM

Brain

Rat

Rat, Wistar, adult, 170 g

Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.

Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
129 , 261 , 1979

Cells

Endothelial

Enzymes

Trypsin : 0.5%

Medium

BSS

Brain

Rat

Rat, Holtzmann, 18 day

Banker, G. and Cowan, M.

Rat Hippocampal Neurons in Dispersed Cell Culture ,
Brain Res
126 , 397 , 1977

Cells

Hippocampal neurons

Enzymes

Trypsin : 0.1%

Medium

HBSS

Brain

Rat

Rat, SD, 2 days

Rowe, V. , Neale, E. , Avins, L. , Guroff, G. and Schrier, B.

Pineal gland cells in culture. Morphology, Biochemistry, Differentiation, and co-culture with sympathetic neurons ,
Exp Cell Res
104 , 345 , 1977

Cells

Pineal

Enzymes

Trypsin : 0.25%

Medium

DMEM, MEM

Brain

Shellfish

Helisoma trivolvis

Hadley, R.D. , Bodnar, D.A. and Kater, S.B.

Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation ,
J Neurosci
5 , 3145 , 1985

Cells

Neurons

Buccal ganglia

Enzymes

Trypsin : 0.2%

Medium

Saline, Sterile

Tumor

Human

Ali, M. , Anand, S. , Tangella, K. , Ramkumar, D. and Saif, T.

Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry. ,
J Vis Exp
, e52532 , 2015

Cells

Colon

Enzymes

Trypsin : 0.1%

Collagenase : 0.1%

Medium

HBSS

Tumor

Human

Hasselbach, L. , Irtenkauf, S. , Lemke, N. , Nelson, K. , Berezovsky, A. , Carlton, E. , Transou, A. , Mikkelsen, T. and deCarvalho, A.

Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry. ,
J Vis Exp
, e51088 , 2014

Cells

Glioblastoma tumor

Enzymes

Trypsin : 0.025%

Collagenase Type 4 : 500 u/ml

Medium

HBSS

Tumor

Human

Dobbin, Z. and Landen, C.

Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
63 , Unit 14.28. , 2013

Cells

Cancer stem

Enzymes

Trypsin : 0.25%

Collagenase : 0.05%

Hyaluronidase : 0.005%

Medium

RPMI

Tumor

Human

Quintana, E. , Shackleton, M. , Foster, H. , Fullen, D. , Sabel, M. , Johnson, T. and Morrison, S.

Phenotypic Heterogeneity Among Tumorigenic Melanoma Cells from Patients that is Reversible and Not Hierarchically Organized. ,
Cancer Cell
18 , 510 , 2010

Cells

Tumorigenic melanoma

Enzymes

Collagenase Type 4 : 200 u/ml

Trypsin : 0.05%

Deoxyribonuclease I : 50-100 u/ml

Medium

PBS

Tumor

Human

MacLeod, R.

Rapid Monolayer Primary Cell Culture from Tissue Biopsy , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons Ltd , 3E:2.1 , 1995

Cells

Epithelial, fibroblasts

Enzymes

Trypsin : 0.25%

Medium

Ham's F-12

Tumor

Human

Sacks, P. , Parnes, S. , Gallick, G. , Mansouri, Z. , Lichtner, R. , Satya-Prakash, K. , Pathak, S. and Parsons, D.

Establishment and Characterization of Two New Squamous Cell Carcinoma Cell Lines Derived from Tumors of the Head and Neck ,
Cancer Res
48 , 2858 , 1988

Cells

Tumor

Enzymes

Trypsin : 0.05%

Medium

DMEM

Tumor

Human

Brattain, M. , Marks, M. , McCombs, J. , Finely, W. and Brattain, D.

Characterization of Human Colon Carcinoma Cell Lines Isolated From a Single Primary Tumour ,
Br J Cancer
47 , 373 , 1983

Cells

Tumor, colon

Enzymes

Trypsin : 0.25%

Medium

McCoy's

Tumor

Mouse

Blanco, S. , Bandiera, R. , Popis, M. , Hussain, S. , Lombard, P. , Aleksic, J. , Sajini, A. , Tanna, H. , Cortes-Garrido, R. , Gkatza, N. , Dietmann, S. and Frye, M.

Stem Cell Function and Stress Response are Controlled by Protein Synthesis. ,
Nature
534 , 335-40 , 2016

Cells

Keratinocytes

Enzymes

Trypsin : 0.25%

Collagenase Type 1 : 0.12%

Collagenase Type 2 : 0.05%

Collagenase Type 4 : 0.05%

Hyaluronidase : 0.1%

Medium

Low calcium containing media

Tumor

Mouse

Chen, X. , Stewart, E. , Shelat, A. , Qu, C. , Bahrami, A. , Hatley, M. , Wu, G. , Bradley, C. , McEvoy, J. , Pappo, A. , Spunt, S. , Valentine, M. , Valentine, V. , Krafcik, F. , Lang, W. , Wierdl, M. and Tsurkan, L.

Targeting Oxidative Stress in Embryonal Rhabdomyosarcoma. ,
Cancer Cell
24 , 710-24 , 2013

Cells

Rhabdomycosarcoma tumor

Enzymes

Trypsin : 0.012%

Collagenase Type 2 : 0.1%

Medium

DMEM

Tumor

Mouse

Mouse, BALB/cfC3H/Crgl

Hosick, H.