Tissue References Search Tool

Bovine

Steers, 1-2 years

Mackintosh, D. and Mason, R.

Pharmacological Actions of 17 Beta-oestradiol on Articular Cartilage Chondrocytes and Chondrosarcoma Chondrocytes in the Absence of Oestrogen Receptors ,
Biochim Biophys Acta
964 , 295 , 1988

Cells

Chondrocytes

Enzymes

Trypsin : 0.20%

Medium

HBSS

Cartilage

Canine

Lee, J. , Kim, H. , Kim, J. , Bae, S. , Joo, D. , Huh, K. , Fang, Y. , Jeong, J. , Kim, M. and Kim, Y.

Microencapsulation of Pancreatic Islets with Canine Ear Cartilage for Immunoisolation. ,
Transplant Proc
44 , 1091-4 , 2012

Cells

Chondrocytes

Enzymes

Collagenase Type 2 : 0.3%

Trypsin : 0.25%

Medium

Ham's F12

Cartilage

Chicken

Chick embryos, HH stage

Wong, M. and Tuan, R.

Nuserum, A Synthetic Serum Replacement, Supports Chondrogenesis of Embryonic Chick Limb Bud Mesenchymal Cells in Micromass Culture ,
In Vitro Cell Dev Biol
29A , 917 , 1993

Cells

Mesenchymal

Enzymes

Trypsin : 0.1%

Medium

DMEM

(see reference)

Cartilage

Chicken

Chicken, broiler strain, 8-10 weeks

Genge, B. , Wu, L. and Wuthier, R.

Differential Fractionation of Matrix Vesicle Proteins: Further Characterization of the Acidic Phospholipid-dependent Ca2+-Binding Proteins ,
J Biol Chem
265 , 4703 , 1990

Cells

Matrix vesicles

Epiphyseal growth plate

Enzymes

Trypsin : 0.1%

Medium

Tris-buffered saline

Cartilage

Chicken

Chicken, broiler strain, 8-10 week

Genge, B.R. , Wu, L.N.Y. and Wuthier, R.E.

Identification of Phospholipid-dependent Calcium-binding Proteins as Constituents of Matrix Vesicles ,
J Biol Chem
264 , 10917 , 1989

Cells

Matrix vesicles

Enzymes

Trypsin : 0.1%

Medium

(see reference)

Cartilage

Chicken

Chick embryos, White Leghorn, 19 day old

Gionti, E. , Capasso, O. and Cancedda, R.

The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions in Vitro ,
J Biol Chem
258 (11) , 7190 , 1983

Cells

Fibroblasts

Epithelial-like

Enzymes

Trypsin : 0.25%

Medium

E 199 medium

Cartilage

Chicken

Chick embryos, 19 day old

Capasso, O. , Gionti, E. , Pontarelli, G. , Ambesi-Impiobato, F. , Nitsch, L. , Tajana, G. and Cancedda, R.

The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions In Vitro ,
Exp Cell Res
142 , 197 , 1982

Cells

Chondrocytes

Enzymes

Trypsin : 0.75%

Medium

Coon's modified F-12

Cartilage

Chicken

Chick embryos, White Leghorn

Ahrens, P. , Solursh, M. and Reiter, R.

Stage-Related Capacity for Limb Chondrogenesis in Cell Culture ,
Dev Biol
60 , 69 , 1977

Cells

Wing buds

Enzymes

Trypsin : 0.1%

Medium

Saline G

Cartilage

Human

Muraglia, A. , Nguyen, V. , Nardini, M. , Mogni, M. , Coviello, D. , Dozin, B. , Strada, P. , Baldelli, I. , Formica, M. , Cancedda, R. and Mastrogiacomo, M.

Culture Medium Supplements Derived from Human Platelet and Plasma: Cell Commitment and Proliferation Support. ,
Front Bioeng Biotechnol
5 , 66 , 2017

Cells

Chondrocytes

Enzymes

Trypsin : 0.25%

Hyaluronidase : 0.1%

Collagenase Type 2 : 1000 u/ml

Collagenase Type 1 : 400 u/ml

Medium

Ham's F12

Cartilage

Human

Human w/ rheumatoid arthritis

Dayer, J. , Krane, S. , Russell, R. and Robinson, D.

Production of Collagenase and Prostaglandins by Isolated Adherent Rheumatoid Synovial Cells ,
Proc Natl Acad Sci U S A
73 (3) , 945 , 1976

Cells

Synovial tissue

Enzymes

Trypsin : 0.05%

Medium

DMEM

(see reference)

Cartilage

Human

Human, 13-62 years

Srivastava, V.M.L. , Malemud, C.J. , Hough, A.J. , Bland, J.H. and Sokoloff, L.

Preliminary Experience with Cell Culture of Human Articular Chondrocytes ,
Arthritis Rheum
17 , 165 , 1974

Cells

Articular chondrocytes

Enzymes

Trypsin : 0.2%

Medium

BSS

Cartilage

Mouse

Mouse, 2-5 day

Otsuru, S. , Hofmann, T. , Raman, P. , Olson, T. , Guess, A. , Dominici, M. and Horwitz, E.

Genomic and Functional Comparison of Mesenchymal Stromal Cells Prepared Using Two Isolation Methods. ,
Cytotherapy
17 , 262-70 , 2015

Cells

Chondrocytes

Enzymes

Trypsin : 0.25%

Collagenase Type 1 : 86.5 u/ml

Medium

DMEM

Cartilage

Rabbit

Rabbit, New Zealand, 1.8-2.3kg

Mehraban F, Tindal MH, Proffitt MM, Moskowitz RW.

Temporal pattern of cysteine endopeptidase (cathepsin B) expression in cartilage and synovium from rabbit knees with experimental osteoarthritis: gene expression in chondrocytes in response to interleukin-1 and matrix depletion ,
Ann Rheum Dis
56 , 108 , 1997

Cells

Chondrocytes

Enzymes

Hyaluronidase : .05%

Collagenase Type 2 : 0.2%

Trypsin : 0.2%

Medium

Gey's solution

Cartilage

Rabbit

Rabbit, white, male, 8 weeks

Benya, P.D. , Padilla, S.R. and Nimni, M.E.

The Progeny of Rabbit Articular Chondrocytes Synthesize Collagen Types I and III and Type I Trimer, but Not Type II ,
Biochemistry
16 , 865 , 1977

Cells

Chondrocytes

Enzymes

Trypsin : 0.2%

Medium

Gey's BSS

Cartilage

Rabbit

Rabbit, New Zealand white or Dutch, 1 week
(also human, newborn)

Schindler, F.H. , Ose, M.A. and Solursh, M.

The Synthesis of Cartilage Collagen by Rabbit and Human Chondrocytes in Primary Cell Culture ,
In Vitro
12 , 44 , 1976

Cells

Chondrocytes

Enzymes

Trypsin : 0.1%

Medium

Saline G, CMF

Cartilage

Rabbit

Rabbit, New Zealand white, immature, 2.25 - 3.3 Kg

Green, J.R. and William, T.

Articular Cartilage Repair. Behavior of Rabbit Chondrocytes During Tissue Culture and Subsequent Allografting ,
Clin Orthop Relat Res
, 237 , 1976

Cells

Articular chondrocytes

Hyaline

Enzymes

Trypsin : 0.2%

Medium

Gey's BSS

Cartilage

Rabbit

Rabbit, New Zealand white, male, 250-350 g

Bentley, G. and Greer , R.

Homotransplantation of Isolated Epiphyseal and Articular Cartilage Chondrocytes into Joint Surfaces of Rabbits ,
Nature
230 , 385 , 1971

Cells

Epiphyseal

Articular cartilage

Enzymes

Trypsin : 0.25%

Medium

Eagle's basal medium

Cartilage

Rat

Mei, L. , Shen, B. , Ling, P. , Liu, S. , Xue, J. , Liu, F. , Shao, H. , Chen, J. , Ma, A. and Liu, X.

Culture-expanded Allogenic Adipose Tissue-derived Stem Cells Attenuate Cartilage Degeneration in an Experimental Rat Osteoarthritis Model. ,
PLoS ONE
12 , e0176107 , 2017

Cells

Chondrocytes

Enzymes

Collagenase Type 2 : 0.15%

Trypsin : 0.25%

Medium

DMEM/F12

Cartilage

Rat

Rat, SD, young, 100-120 g

Shimomura, Y. , Yoneda, T. and Suzuki, F.

Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib ,
Calcif Tissue Res
19 , 179 , 1975

Cells

Chondrocytes

Enzymes

Trypsin : 0.2%

Medium

Ham's F-12 medium

Kidney

Fish

Winter flounder (Pseudopleur-onectes americanus)
200 - 500 g

Dickman, K. and Renfro, J.

Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport ,
Am J Physiol
251 , 424 , 1986

Cells

Renal tubule

Enzymes

Trypsin : 0.2%

Medium

CMF solution

Kidney

Guinea-Pig

Guinea-pig, male, 100 g

Phillips, H.

Dissociation of Single Cells from Lung or Kidney Tissue with Elastase ,
In Vitro
8 , 101 , 1972

Cells

Single cells

Enzymes

Trypsin : 0.25%

Medium

CF salt solution

Kidney

Human

McAteer, J, Kempson, S. and Evan, A

Culture of Human Renal Cortex Epithelial Cells ,
J Tiss Cul Meth
13 , 143 , 1991

Cells

Renal cortex

Enzymes

Trypsin : 0.1%

Medium

Tissue Culture Grade Water

Kidney

Human

Heieren, M. , van der Woude, F. and Balfour Jr., H.

Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells ,
Proc Natl Acad Sci U S A
85 , 1642 , 1988

Cells

Mesangial

Enzymes

Trypsin : 0.25%

Medium

DMEM/Ham's F-12

Kidney

Human

Human, 24 newborn and stillborn
(also rabbit)

De Oca, H. and Malinin, T.

Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys ,
J Clin Microbiol
2 , 243 , 1975

Cells

Renal

Enzymes

Trypsin : 0.25%

Medium

See reference

Kidney

Monkey

Melnick, J. and Wallis, C.

Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells ,
Dev Biol
37 , 77 , 1976

Cells

Kidney

Enzymes

Trypsin : 0.01% - 0.00001%

Medium

Eagle's MEM

Kidney

Mouse

Mouse, 3-7 month

Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.

Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
10 , 1569-79 , 1999

Cells

Fibroblasts, mesangial, smooth muscle

Enzymes

Trypsin : 0.25%

Collagenase : see reference

Soybean Trypsin Inhibitor : 0.05%

Medium

DMEM

Kidney

Rabbit

Rabbit, New Zealand, white

Grenier, F. and Smith, W.

Formation of 6-keto-PGF-1aby Collecting Tubule Cells Isolated from Rabbit Renal Papillae ,
Prostaglandins
16 , 759 , 1978

Cells

Collecting tubule

Enzymes

Trypsin : 0.05%

Medium

Kreb's Ringer buffer

Kidney

Rat

Wallach, D. , Anderson, W. and Pastan, I.

Activation of Adenylate Cyclase in Cultured Fibroblasts by Trypsin ,
J Biol Chem
253 , 24 , 1978

Cells

Fibroblasts

Kidney

Enzymes

Trypsin : up to 0.25%

Medium

Dulbecco-Vogt MEM

Tissue Tables (references, grouped by tissue type and species)

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Tissue References Search Tool

Tissue Tables (references, grouped by tissue type and species)