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Tissue References Search Tool

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Liver
Canine
Dog, adult
Vickrey, H. , Ramon, J. and McCann, D.
Continuous Culture of Normal Adult Mammalian Hepatocytes Exhibiting Parenchymal Functions ,
In Vitro
 15 , 120 , 1979
Cells
Hepatocytes
Enzymes
Trypsin : 0.1%
Medium
HBSS, CMF
Liver
Chicken
Chick embryos, 5 day
Steinberg, M.
"ECM": Its Nature, Origin, And Function in Cell Aggregation ,
Exp Cell Res
 30 , 257 , 1963
Cells
Heart
Liver
Enzymes
Trypsin : 3.0%
Medium
Tyrode's solution, CMF
Liver
Chicken
Chick embryonic
Rinaldini, L.
An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells ,
Exp Cell Res
 16 , 477 , 1959
Cells
Various tissues (heart, liver, skeletal, cardiac)
Enzymes
Trypsin : various grades
Medium
CMF Tyrode's solution
Liver
Human
Human
Kaighn, M.
Human Liver Cells , Tissue Culture Methods / Applications , (Kruse, P., Patterson, M. eds) , 54 , 1973
Cells
Hepatocytes
Enzymes
Trypsin : 0.1%
Medium
HBSS, CMF
Liver
Monkey
Monkey (Macaca fascicularis), adult, 5-6 Kg
Ulrich, R. , Aspar, D. , Cramer, C. , Kletzien, R. and Ginsberg, L.
Isolation and Culture of Hepatocytes from the Cynogmolgus Monkey (Macaca fascicularis) ,
In Vitro Cell Dev Biol
 26 , 815 , 1990
Cells
Hepatocytes
Enzymes
Trypsin : 160 u/ml
Medium
HEPES buffer
Liver
Rat
Rat, Wistar, adult, male, 150-200 g
Okumura, T. and Saito, K.
Degradation of Prostaglandin E2 in a Primary Culture of Adult Rat Hepatocytes ,
J. Biochem.
 96 , 429 , 1984
Cells
Hepatocytes
Enzymes
Trypsin : 0.005%
Medium
Williams E
Liver
Rat
Rat, Fischer, Lewis and SD, male 10-18 months
Herring, A. , Raychaudhuri, R. , Kelley, S. and Iybe, P.
Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats ,
In Vitro
 19 , 576 , 1983
Cells
Epithelial
Enzymes
Trypsin : 0.05%
Medium
HBSS CMF
Liver
Rat
Rat, Wistar, 12 day
Malan-Shibley, L. and Iype, P.
Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels ,
Exp Cell Res
 131 , 363 , 1981
Cells
Epithelial
Enzymes
Trypsin : 0.05%
Medium
HBSS, CMF
Liver
Rat
Rat, neonate, 3 day
Bausher, J. and Schaeffer, W.
A Diploid Rat Liver Cell Culture. 1. Characterization and Sensitivity to Aflatoxin B1 ,
In Vitro
 9 , 286 , 1974
Cells
Hepatocytes
Enzymes
Trypsin : 0.25%
Medium
PBS, CMF
Liver
Rat
Rat, Fisher, 10 day
Williams, G. , Weisburger, E. and Weisburger, J.
Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver ,
Exp Cell Res
 69 , 106 , 1971
Cells
Epithelial-like
Enzymes
Trypsin : 0.25%
Medium
PBS
Tumor
Human
Human
Ali, M. , Anand, S. , Tangella, K. , Ramkumar, D. and Saif, T.
Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry. ,
J Vis Exp
 , e52532 , 2015
Cells
Colon
Enzymes
Trypsin : 0.1%
Collagenase : 0.1%
Medium
HBSS
Tumor
Human
Human
Hasselbach, L. , Irtenkauf, S. , Lemke, N. , Nelson, K. , Berezovsky, A. , Carlton, E. , Transou, A. , Mikkelsen, T. and deCarvalho, A.
Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry. ,
J Vis Exp
 , e51088 , 2014
Cells
Glioblastoma tumor
Enzymes
Trypsin : 0.025%
Collagenase Type 4 : 500 u/ml
Medium
HBSS
Tumor
Human
Human
Dobbin, Z. and Landen, C.
Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
 63 , Unit 14.28. , 2013
Cells
Cancer stem
Enzymes
Trypsin : 0.25%
Collagenase : 0.05%
Hyaluronidase : 0.005%
Medium
RPMI
Tumor
Human
Human
Quintana, E. , Shackleton, M. , Foster, H. , Fullen, D. , Sabel, M. , Johnson, T. and Morrison, S.
Phenotypic Heterogeneity Among Tumorigenic Melanoma Cells from Patients that is Reversible and Not Hierarchically Organized. ,
Cancer Cell
 18 , 510 , 2010
Cells
Tumorigenic melanoma
Enzymes
Collagenase Type 4 : 200 u/ml
Trypsin : 0.05%
Deoxyribonuclease I : 50-100 u/ml
Medium
PBS
Tumor
Human
Human
MacLeod, R.
Rapid Monolayer Primary Cell Culture from Tissue Biopsy , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons Ltd , 3E:2.1 , 1995
Cells
Epithelial, fibroblasts
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Tumor
Human
Human
Sacks, P. , Parnes, S. , Gallick, G. , Mansouri, Z. , Lichtner, R. , Satya-Prakash, K. , Pathak, S. and Parsons, D.
Establishment and Characterization of Two New Squamous Cell Carcinoma Cell Lines Derived from Tumors of the Head and Neck ,
Cancer Res
 48 , 2858 , 1988
Cells
Tumor
Enzymes
Trypsin : 0.05%
Medium
DMEM
Tumor
Human
Human
Brattain, M. , Marks, M. , McCombs, J. , Finely, W. and Brattain, D.
Characterization of Human Colon Carcinoma Cell Lines Isolated From a Single Primary Tumour ,
Br J Cancer
 47 , 373 , 1983
Cells
Tumor, colon
Enzymes
Trypsin : 0.25%
Medium
McCoy's
Tumor
Mouse
Mouse
Blanco, S. , Bandiera, R. , Popis, M. , Hussain, S. , Lombard, P. , Aleksic, J. , Sajini, A. , Tanna, H. , Cortes-Garrido, R. , Gkatza, N. , Dietmann, S. and Frye, M.
Stem Cell Function and Stress Response are Controlled by Protein Synthesis. ,
Nature
 534 , 335-40 , 2016
Cells
Keratinocytes
Enzymes
Medium
Low calcium containing media
Tumor
Mouse
Mouse
Chen, X. , Stewart, E. , Shelat, A. , Qu, C. , Bahrami, A. , Hatley, M. , Wu, G. , Bradley, C. , McEvoy, J. , Pappo, A. , Spunt, S. , Valentine, M. , Valentine, V. , Krafcik, F. , Lang, W. , Wierdl, M. and Tsurkan, L.
Targeting Oxidative Stress in Embryonal Rhabdomyosarcoma. ,
Cancer Cell
 24 , 710-24 , 2013
Cells
Rhabdomycosarcoma tumor
Enzymes
Trypsin : 0.012%
Medium
DMEM
Tumor
Mouse
Mouse, BALB/cfC3H/Crgl
Hosick, H.
A Note on Growth Patterns of Epithelial Tumor Cells in Primary Culture ,
Cancer Res
 34 , 259 , 1974
Cells
Neoplastic
Epithelial tumor
Medium
DMEM
Tumor
Rat
Rat
Brennan, M. , Oldberg, A. , Hayman, E. and Ruoslahti, E.
Effect of a Proteoglycan Produced by Rat Tumor Cells on Their Adhesion to Fibronectin-Collagen Substrata ,
Cancer Res
 43 , 4302 , 1983
Cells
Yolk sac tumor
Enzymes
Trypsin : 0.01%
Medium
DMEM
Tumor
Rat
Rat
Gazdar, A. , Chick, W. , Oie, H. , Sims, H. , King, D. , Weir, G. and Lauris, V.
Continuous, Clonal, Insulin-and Somatostatin-Secreting Cell Lines Established from a Transplantable Rat Islet Cell Tumor ,
Proc Natl Acad Sci U S A
 77 (6) , 3519 , 1980
Cells
Tumor, islet
Enzymes
Trypsin : 0.05%
Medium
Medium 199
Tumor
Rat
Rat
Essner, E.
Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands ,
Exp Cell Res
 7 , 430 , 1954
Cells
Ascites hepatoma
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer (see reference)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.