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Tissue References Search Tool

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Kidney
Fish
Winter flounder (Pseudopleur-onectes americanus)
200 - 500 g
Dickman, K. and Renfro, J.
Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport ,
Am J Physiol
 251 , 424 , 1986
Cells
Renal tubule
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
CMF solution
Kidney
Guinea-Pig
Guinea-pig, male, 100 g
Phillips, H.
Dissociation of Single Cells from Lung or Kidney Tissue with Elastase ,
In Vitro
 8 , 101 , 1972
Cells
Single cells
Enzymes
Trypsin : 0.25%
Medium
CF salt solution
Kidney
Human
Human
McAteer, J, Kempson, S. and Evan, A
Culture of Human Renal Cortex Epithelial Cells ,
J Tiss Cul Meth
 13 , 143 , 1991
Cells
Renal cortex
Enzymes
Trypsin : 0.1%
Medium
Tissue Culture Grade Water
Kidney
Human
Human
Heieren, M. , van der Woude, F. and Balfour Jr., H.
Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells ,
Proc Natl Acad Sci U S A
 85 , 1642 , 1988
Cells
Mesangial
Enzymes
Trypsin : 0.25%
Medium
DMEM/Ham's F-12
Kidney
Human
Human, 24 newborn and stillborn
(also rabbit)
De Oca, H. and Malinin, T.
Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys ,
J Clin Microbiol
 2 , 243 , 1975
Cells
Renal
Enzymes
Trypsin : 0.25%
Medium
See reference
Kidney
Monkey
Monkey
Melnick, J. and Wallis, C.
Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells ,
Dev Biol
 37 , 77 , 1976
Cells
Kidney
Enzymes
Trypsin : 0.01% - 0.00001%
Medium
Eagle's MEM
Kidney
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Kidney
Rabbit
Rabbit, New Zealand, white
Grenier, F. and Smith, W.
Formation of 6-keto-PGF-1aby Collecting Tubule Cells Isolated from Rabbit Renal Papillae ,
Prostaglandins
 16 , 759 , 1978
Cells
Collecting tubule
Enzymes
Trypsin : 0.05%
Medium
Kreb's Ringer buffer
Kidney
Rat
Rat
Wallach, D. , Anderson, W. and Pastan, I.
Activation of Adenylate Cyclase in Cultured Fibroblasts by Trypsin ,
J Biol Chem
 253 , 24 , 1978
Cells
Fibroblasts
Kidney
Enzymes
Trypsin : up to 0.25%
Medium
Dulbecco-Vogt MEM
Pancreas
Bovine
Bovine
Stiles, G. and Lefkowitz, R.
Hormone-Sensitive Adneylate Cyclase ,
J Biol Chem
 257 (11) , 6287 , 1982
Cells
Platelets
Medium
(see reference)
Pancreas
Canine
Canine
Zoran, M. , Doyle, R. and Haydon, P.
Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis ,
Neuron
 6 , 145 , 1991
Cells
Buccal ganglia; SLT muscle
Enzymes
Trypsin : 0.2%
Medium
DMEM
Pancreas
Human
Human
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Pancreas
Rat
Rat, SD, male, 100 - 150 g
Yeh, C. , Mertz, P. , Oliver, C. , Baum, B. and Kousvelari, E.
Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells ,
In Vitro Cell Dev Biol
 27 , 707 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.001%
Medium
F12 medium
Pancreas
Rat
Rat, Wistar, male
Foskett, J. , Roifman, C. and Wong, D.
Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells ,
J Biol Chem
 266 , 2778 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.02%
Medium
Solution B
(See reference)
Pancreas
Rat
Rat, Fischer-344, either sex, 120-150 g
Tsao, M. and Duguid, W.
Establishment of Propagable Epithelial Cell Lines From Normal Adult Rat Pancreas ,
Exp Cell Res
 168 , 365 , 1987
Cells
Epithelial
Enzymes
Trypsin : 0.1%
Medium
Ham's F-12/HBSS
(see reference)
Pancreas
Rat
Rat, SD, male, 50 - 125 g
Oliver, C. , Waters, J. , Tolbert, C. and Kleinman, H.
Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel ,
In Vitro Cell Dev Biol
 23 , 465 , 1987
Cells
Acinar
Exorbital lacrimal, parotid, pancreas
Enzymes
Trypsin : 0.01%
Medium
HBSS, CMF
Pancreas
Rat
Rat, SD, 125 - 350 g
Githens, S. , Holmquist, D. , Whelan, J. and Ruby, J.
Ducts of the Rat Pancreas in Agarose Matrix Culture ,
In Vitro
 16 , 797 , 1980
Cells
Duct
Enzymes
Trypsin : 0.01%
Medium
HBSS
Pancreas
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF
Pancreas
Rat
Rat, neonate
Leonard, R. , Lazarow, A. and Hegre, O.
Pancreatic Islet Transplantation in the Rat ,
Diabetes
 22 , 413 , 1973
Cells
Islets
Enzymes
Trypsin : 0.05%
Medium
Puck's saline buffered w/ EDTA 0.02%
Pancreas
Rat
Rat
Essner, E.
Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands ,
Exp Cell Res
 7 , 430 , 1954
Cells
Ascites hepatoma
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer (see reference)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.