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Adipose/Fat
Bovine

Belgian Blue, 1-7 year

Mitić, R. , Cantoni, F. , Börlin, C. , Post, M. and Jackisch, L.
A Simplified and Defined Serum-free Medium for Cultivating Fat Across Species. , iScience Vol. 26 , 105822 , 2023
Cells
Stomal vascular cells
Medium
Growth medium
Adipose/Fat
Bovine
Bovine
Yang, Y. and Baldwin, R.
Preparation and Metabolism of Isolated Cells from Bovine Adipose Tissue ,
J Dairy Sci
 56 , 350 , 1973
Cells
Adipocytes
Enzymes
Collagenase Type 1 : 40 u/ml
Medium
Krebs-Ringer bicarbonate
Adipose/Fat
Porcine

Porcine, 2-6 year

Mitić, R. , Cantoni, F. , Börlin, C. , Post, M. and Jackisch, L.
A Simplified and Defined Serum-free Medium for Cultivating Fat Across Species. , iScience Vol. 26 , 105822 , 2023
Cells
Stomal vascular cells
Medium
Growth medium
Adipose/Fat
Porcine
Porcine, female, <1 year
Williams, K. , Picou, A. , Kish, S. , Giraldo, A. , Godke, R. and Bondioli, K
Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells. ,
Cells Tissues Organs
 188 , 251 , 2008
Cells
Adipose mesenchymal stem
Enzymes
Medium
DMEM
Adipose/Fat
Porcine
Porcine, crossbred, male 1-4 day
Ramsay, T.G.
Porcine Leptin Inhibits Lipogenesis in Porcine Adipocytes ,
J Anim Sci
 81 , 3008 , 2003
Cells
Adipocytes
Enzymes
Medium
DMEM/F12
Adipose/Fat
Porcine
Porcine, crossbred
Liang, W. and Mills, S.
Quantitative Analysis of beta-Adrenergic Receptor Subtypes in Pig Tissues. ,
J Anim Sci
 80 , 963-70 , 2002
Cells
Adipocytes
Enzymes
Medium
Krebs-Ringer
Adipose/Fat
Porcine
Porcine, neonatal
Wang Y, Fried SK, Petersen RN, Schoknecht PA.
Somatotropin regulates adipose tissue metabolism in neonatal swine ,
J Nutr
 129 , 139-45 , 1999
Cells
Adipocytes
Enzymes
Medium
Krebs-Ringer bicarbonate albumin
Adipose/Fat
Porcine
Porcine, 8-9 week
Ding, S. , McNeel, R. and Mersmann, H.
Expression of Porcine Adipocyte Transcripts: Tissue Distribution and Differentiation In Vitro and In Vivo ,
Comp Biochem Physiol B
 123 , 307 , 1999
Cells
Adipose
Enzymes
Collagenase Type 1 : 300 u/ml
Medium
HEPES
Adipose/Fat
Porcine
Porcine, crossbred, 1-3 day
Suryawan, A. , Swanson, L. and Hu, C.
Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture ,
J Anim Sci
 75 , 105 , 1997
Cells
Adipose, Stromal-vascular
Enzymes
Medium
DMEM/F12
Adipose/Fat
Squirrel
Squirrel
McFarlane, S. , Mathers, K. and Staples, J.
Reversible Temperature-Dependent Differences in Brown Adipose Tissue Respiration During Torpor in a Mammalian Hibernator. ,
Am J Physiol Regul Integr Comp Physiol
 312 , R434-R442 , 2017
Cells
Brown adipocytes
Enzymes
Medium
Krebs-Ringer phosphate
Adrenal
Bovine
Bovine
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Bovine
Bovine, 6 month
Moustafa T, Girod S, Tortosa F, Li R, Sol JC, Rodriguez F, Bastide R, Lazorthes Y, Sallerin B.
Viability and functionality of bovine chromaffin cells encapsulated into alginate-PLL microcapsules with a liquefied inner core ,
Cell Transplant
 15 , 121-33 , 2006
Cells
Chromaffin
Enzymes
Medium
Locke's solution
Adrenal
Bovine
Bovine
Hahm, S. , Chen, Y. , Vinson, C. and Eiden, L.
A Calcium-Initiated Signaling Pathway Propagated Through Calcineurin and cAMP Response Element-Binding Protein Activates Proenkephalin Gene Transcription after Depolarization. ,
Mol Pharmacol
 64 , 1503 , 2003
Cells
Chromaffin cells
Enzymes
Collagenase : 0.1%
Medium
DMEM
Adrenal
Bovine
Bovine
Ortega, J. , Sagen, J. and Pappas, G.
Short-term Immunosuppression Enhances Long-term Survival of Bovine Chromaffin Cell Xenografts in Rat CNS ,
Cell Transplant
 1 , 33 , 1992
Cells
Chromaffin
Enzymes
Collagenase : 0.2%
Medium
Locke's solution
Adrenal
Bovine
Bovine
Zhu, J. , Li, W. , Toews, M. and Hexum, T.
Neuropeptide Y Inhibits Forskolin-Stimulated Adenylate Cyclase in Bovine Adrenal Chromaffin Cells via a Pertussis Toxin-Sensitive Process ,
J Pharmacol Exp Ther
 263 (3) , 1479 , 1992
Cells
Chromaffin
Enzymes
Medium
HEPES
Adrenal
Bovine
Bovine
(also rat)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Adrenal
Bovine
Bovine
Dahmer, M. , Hart, P. and Perlman, R.
Studies on the Effect of Insulin-Like Growth Factor-I on Catecholamine Secretion from Chromaffin Cells ,
J Neurochem
 54 (3) , 931 , 1990
Cells
Chromaffin
Enzymes
Medium
DMEM
Adrenal
Bovine
Bovine
Higgins, L. and Berg, D.
Immunological Identification of a Nicotinic Acetylcholine Receptor on Bovine Chromaffin Cells ,
J Neurosci
 7 (6) , 1792 , 1987
Cells
Chromaffin
Enzymes
Medium
Locke's solution
Adrenal
Bovine
Bovine
Aunis, D. , Rotllan, P. and Miras-Portugal, M.
Incorporation of Adenosine into Nucleotides of Chromaffin Cells in Culture ,
Neurochem Int
 7 , 89 , 1985
Cells
Chromaffin
Enzymes
Collagenase : 0.05%
Medium
Locke's solution, CMF
Adrenal
Bovine
Bovine
Almazan, G. , Aunis, D. , Garcia, A. , Montiel, C. , Nicolas, G. and Sanchez-Garcia, P.
Effects Of CLS on the Release of Noradrenaline From Chromaffin Cells ,
Br J Biomed Sci
 81 , 599 , 1984
Cells
Chromaffin
Enzymes
Collagenase : 0.15%
Medium
Kreb's, CMF
Adrenal
Bovine
Bovine
Pollard, H. , Pazoles, C. , Creutz, C. , Scott, J. , Zinder, O. and Hotchkiss, A.
An Osmotic Mechanism For Exocytosis From Dissociated Chromaffin Cells ,
J Biol Chem
 259 , 1114 , 1984
Cells
Chromaffin
Enzymes
Collagenase : 0.1%
Medium
(see reference)
Adrenal
Bovine
Bovine
Cena, V. , Garcia, A. , Montiel, C. and Sanchez-Garcia, P.
Uptake of [3H]-nicotine and [3H]-noradrenaline by Cultured Chromaffin Cells ,
Br J Pharmacol
 81 , 119 , 1984
Cells
Chromaffin
Enzymes
Medium
CF Kreb's
Adrenal
Bovine
Bovine
Knight, D. and Baker, P.
Stimulus-Secretion Coupling in Isolated Bovine Adrenal Medullary Cells ,
Q J Exp Physiol
 68 , 123 , 1983
Cells
Medulla
Enzymes
Hyaluronidase : 0.2%
Medium
Saline w/BSA 0.5%
Adrenal
Bovine
Bovine
Waymire, J. , Bennett, W. , Boehme, R. , Hankins, L. , Gilmer-Waymire, K. and Haycock, J.
Bovine Adrenal Chromaffin Cells: High-Yield Purification and Viability in Suspension Culture ,
J Neurosci Methods
 7 , 329 , 1983
Cells
Chromaffin
Enzymes
Medium
HBSS, modified
Adrenal
Bovine
Bovine
Greenberg, A. and Zinder, O.
alpha- and beta-Receptor Control of Catecholamine Secretion from Isolated Adrenal Medulla Cells ,
Cell Tissue Res
 226 , 655 , 1982
Cells
Medulla
Enzymes
Collagenase : 0.2%
Medium
Krebs-Ringer bicarbonate buffer, CMF
Adrenal
Bovine
Bovine
Wilson, S.P. and Viveros, O.H.
Primary Culture of Adrenal Medullary Chromaffin Cells in a Chemically Defined Medium ,
Exp Cell Res
 133 , 159 , 1981
Cells
Chromaffin
Enzymes
Deoxyribonuclease I : 15 µg/ml
Medium
Medium A
(see reference)
Adrenal
Bovine
Bovine
Baker, P. and Knight., D
Calcium Control of Exocytosis and Endocytosis in Bovine Adrenal Medullary Cells ,
Phil Trans R Soc Lond
 296 , 83 , 1981
Cells
Medulla
Enzymes
Protease : 0.2%
Medium
Saline
Adrenal
Bovine
Bovine
Kilpatrick, D. , Ledbetter, F. , Carson, K. , Kirshner, A. , Slepetis, R. and Kirshner, N.
Stability of Bovine Adrenal Medulla Cells in Culture ,
J Neurochem
 35 (3) , 679 , 1980
Cells
Medulla
Enzymes
Collagenase : 0.05%
Medium
Locke's solution, CF
Adrenal
Bovine
Bovine
Aunis, D. , Guerold, B. , Bader, M-F. and Cieselski-Treska, J.
Immunocytochemical and Biochemical Demonstration of Contractile Proteins in Chromaffin Cells ,
Neuroscience
 5 , 2261 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.05%
Medium
DMEM
Adrenal
Bovine
Bovine
Trifaro, J.M. and Lee, R.W.
Morphological Characteristics and Stimulus-Secretion Coupling in Bovine Adrenal Chromaffin Cell Cultures ,
Neuroscience
 5 , 1533 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.05%
Medium
Locke's solution, CMF
Adrenal
Bovine
Bovine, adult
(also rat, Hanover-Wistar, young; guinea-pig, newborn)
Unsicker, K. , Rieffert, B. and Ziegler, W.
Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro ,
Adv Biochem Psychopharmacol
 255 , 51 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.5%
Collagenase : 0.5%
Medium
HBSS
Adrenal
Bovine
Bovine
Kumakura, K. , Karoum, F. , Guidotti, A. and Costa, E.
Modulation of Nicotinic Receptors by Opiate Receptor Agonists in Cultured Adrenal Chromaffin Cells ,
Nature
 283 , 489 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.25%
Medium
F-12 medium
Adrenal
Bovine
Bovine, adult
Unsicker, K. and Griesser, G.
Establishment, Characterization and Fibre Outgrowth of Isolated Bovine Adrenal Medullary Cells in Long-Term Cultures ,
Neuroscience
 5 , 1445 , 1980
Cells
Medulla
Enzymes
Medium
HBSS
Adrenal
Bovine
Bovine
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Adrenal
Bovine
Bovine
Hersey, R. and DiStefano, V.
Control of Phenylethanolamine N-Methyltransferase by Glucocorticoids in Cultured Bovine Adrenal Medullary Cells ,
J Pharmacol Exp Ther
 209 (1) , 147 , 1979
Cells
Medullary
Enzymes
Medium
HEPES
Adrenal
Bovine
Bovine
Fenwick, E. , Fajdiga, P. , Howe, N. and Livett, B.
Functional and Morphological Characterization of Isolated Bovine Adrenal Medullary Cell ,
J Cell Biol
 76 , 12 , 1978
Cells
Medulla
Enzymes
Medium
Kreb's, CF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.