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Tissue References Search Tool

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Adipose/Fat
Canine
23-33 kg
Williams, S. , Kosnik, P. , Kleinert, L. , Vossman, E. , Lye, K. and Shine, M.
Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts. ,
Tissue Eng Part A
 19 , 1295-302 , 2013
Cells
Stromal vascular
Enzymes
Collagenase : see reference
Medium
Media 199E
Adipose/Fat
Canine
Canine
Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J.
Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. ,
Lipids Health Dis
 10 , 171 , 2011
Cells
Renal adipose derived cells
Enzymes
Medium
DMEM
Adipose/Fat
Canine
Canine, 20-25 kg
Fischer, L. , McIlhenny, S. , Tulenko, T. , Golesorkhi, N. , Zhang, P. , Larson, R. , Lombardi, J. , Shapiro, I. and DiMuzio, P.
Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force. ,
J Surg Res
 152 , 157 , 2009
Cells
Adipose stem cells
Enzymes
Collagenase : see reference
Medium
Media-199
Adipose/Fat
Canine
Canine
DiGirolamo, M. , Mendlinger, S. and Fertig, J.W.
A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species ,
Am J Physiol
 221 , 850 , 1971
Cells
White fat
Enzymes
Collagenase : 0.05%
Medium
Kreb's Ringer bicarbonate buffer
Adipose/Fat
Guinea-Pig
Guinea-pig, adult
(also rat, hamster, gerbil, rabbit, lamb)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adrenal
Guinea-Pig
Guinea-pig, 500-700 g
Role, L.W. , Leeman, S.E. and Perlman, R.L.
Somatostain and Substance P Inhibit Catecholamine Secretion from Isolated Cells of Guinea-pig Adrenal Medulla ,
Neurochem Int
 6 , 1813 , 1981
Cells
Chromaffin
Medulla
Medium
BSS
(see reference)
Adrenal
Guinea-Pig
Guinea-pig
(also rat, Hanover-Wistar, young; newborn; cattle)
Unsicker, K. , Rieffert, B. and Ziegler, W.
Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro ,
Adv Biochem Psychopharmacol
 255 , 51 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.5%
Collagenase : 0.5%
Medium
HBSS
Adrenal
Guinea-Pig
Guinea-pig
Hochman, J. and Perlman, R.L.
Catecholamine Secretion by Isolated Adrenal Cells ,
Biochim Biophys Acta
 421 , 168 , 1976
Cells
Adrenal
Chromaffin
Enzymes
Collagenase : 0.05%-0.20%
Medium
Kreb's-Ringer bicarb glucose buffer, CF
Bone
Chicken
Chick, day old
Gay, C. , Lloyd, Q. and Gilman, V.
Characteristics and Culture of Osteoblasts Derived From Avian Long Bone ,
In Vitro Cell Dev Biol
 30A , 379 , 1994
Cells
Osteoblasts
Enzymes
Trypsin : 0.03%
Medium
DMEM
Bone
Chicken
Chick, Peterson/Arbor Acre, male, 4 weeks old (Gallus domesticus)
Rosselot, G. , Reginato, A.M. and Leach, R.M.
Development of a Serum-Free System to Study the Effect of Growth Hormone and Insulinlike Growth Factor-1 on Cultured Postembryonic Growth Plate Chondrocytes ,
In Vitro Cell Dev Biol
 28A , 235 , 1992
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Medium
Ham's F12
Bone
Chicken
Chick, embryo
Schiltz, J.R. , Mayne R. and Holtzer, H.
The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture ,
Differentiation
 1 , 97 , 1973
Cells
Vertebrae chondroblasts
Enzymes
Trypsin : 0.25%
Medium
Simm's, CMF
Brain
Guinea-Pig
Guinea-pig, 200-400 g
Kay, A.R. and Wong, R.K.S.
Isolation of Neurons Suitable for Patch-Clamping from Adult Mammalian Central Nervous Systems ,
J Neurosci Methods
 16 , 227 , 1986
Cells
Neurons
Enzymes
Trypsin : 0.06-0.08%
Medium
PIPES saline
Cartilage
Canine
Canine
Lee, J. , Kim, H. , Kim, J. , Bae, S. , Joo, D. , Huh, K. , Fang, Y. , Jeong, J. , Kim, M. and Kim, Y.
Microencapsulation of Pancreatic Islets with Canine Ear Cartilage for Immunoisolation. ,
Transplant Proc
 44 , 1091-4 , 2012
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Medium
Ham's F12
Cartilage
Chicken
Chick embryos, HH stage
Wong, M. and Tuan, R.
Nuserum, A Synthetic Serum Replacement, Supports Chondrogenesis of Embryonic Chick Limb Bud Mesenchymal Cells in Micromass Culture ,
In Vitro Cell Dev Biol
 29A , 917 , 1993
Cells
Mesenchymal
Enzymes
Trypsin : 0.1%
Medium
DMEM
(see reference)
Cartilage
Chicken
Chicken, broiler strain, 8-10 weeks
Genge, B. , Wu, L. and Wuthier, R.
Differential Fractionation of Matrix Vesicle Proteins: Further Characterization of the Acidic Phospholipid-dependent Ca2+-Binding Proteins ,
J Biol Chem
 265 , 4703 , 1990
Cells
Matrix vesicles
Epiphyseal growth plate
Enzymes
Trypsin : 0.1%
Medium
Tris-buffered saline
Cartilage
Chicken
Chicken, broiler strain, 8-10 week
Genge, B.R. , Wu, L.N.Y. and Wuthier, R.E.
Identification of Phospholipid-dependent Calcium-binding Proteins as Constituents of Matrix Vesicles ,
J Biol Chem
 264 , 10917 , 1989
Cells
Matrix vesicles
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Cartilage
Chicken
Chick embryos, White Leghorn, 19 day old
Gionti, E. , Capasso, O. and Cancedda, R.
The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions in Vitro ,
J Biol Chem
 258 (11) , 7190 , 1983
Cells
Fibroblasts
Epithelial-like
Enzymes
Trypsin : 0.25%
Medium
E 199 medium
Cartilage
Chicken
Chick embryos, 19 day old
Capasso, O. , Gionti, E. , Pontarelli, G. , Ambesi-Impiobato, F. , Nitsch, L. , Tajana, G. and Cancedda, R.
The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions In Vitro ,
Exp Cell Res
 142 , 197 , 1982
Cells
Chondrocytes
Enzymes
Trypsin : 0.75%
Medium
Coon's modified F-12
Cartilage
Chicken
Chick embryos, White Leghorn
Ahrens, P. , Solursh, M. and Reiter, R.
Stage-Related Capacity for Limb Chondrogenesis in Cell Culture ,
Dev Biol
 60 , 69 , 1977
Cells
Wing buds
Enzymes
Trypsin : 0.1%
Medium
Saline G
Colon
Guinea-Pig
Guinea-pig, adult
Raghupathi, R. , Duffield, M. , Zelkas, L. , Meedeniya, A. , Brookes, S. , Sia, T. , Wattchow, D. , Spencer, N. and Keating, D.
Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells. ,
J Physiol
 591 , 5959-75 , 2013
Cells
Enterochromaffin
Enzymes
Trypsin : 0.05%
Medium
DMEM
Colon
Guinea-Pig
Guinea-Pig, male, 200-250g
Kang, M. , Maguma, H. , Smith, T. , Ross, G. , Dewey, W. and Akbarali, H.
The Role of β-arrestin2 in the Mechanism of Morphine Tolerance in the Mouse and Guinea Pig Gastrointestinal Tract. ,
J Pharmacol Exp Ther
 340 , 567-76 , 2012
Cells
Myenteric ganglia
Enzymes
Protease : 0.1%
Medium
Kreb's solution
Endothelial
Canine
Dog
(also human)
Gerhart, D. Z. , Broderius, M. A. and Drewes, L. R.
Cutlured Human and Canine Endothelial Cells from Brain Microvessels ,
Brain Res Bull
 21 , 785 , 1988
Cells
Microvessels
Enzymes
Medium
DMEM
Endothelial
Canine
Dog, mongrel, adult
Ford, J. , Burkel, W. and Kahn, R.
Isolation of Adult Canine Venous Endothelium for Tissue Culture ,
In Vitro
 17 , 44 , 1981
Cells
Endothelium
Jugular vein
Enzymes
Trypsin : 0.1%
Medium
Earle's PBS, CMF
Endothelial
Guinea-Pig
Guinea pig, female, 300-350g
Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J.
Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium ,
Am J Physiol Heart Circ Physiol
 281 , H1657-66 , 2001
Cells
Coronary endothelial
Enzymes
Medium
see reference
Epithelial
Canine
Dog
Virmani, A. , Naziruddin, B. , Desai, V. , Lowry, J. , Graves, D. and Sachdev, G.
Evidence for Secretion of High Molecular Weight Mucins by Canine Tracheal Epithelial Cells in Primary Culture: Effects of Select Secretagogues on Mucin Secretion ,
In Vitro Cell Dev Biol
 28A , 120 , 1992
Cells
Tracheal
Enzymes
Pronase : 0.1%
Medium
DMEM
Epithelial
Chicken
Chick, 5 day old
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Cells
Intestinal mesenchymal and epithelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Epithelial
Guinea-Pig
Guinea-pig, Hartley, female, 200 g
Rutten, M.
Use of Commerically Available Cell Culture Inserts for Primary Culture and Electrophysiologic Studies of Guinea Pig Gastric Mucous Epithelial Cells ,
J Tiss Cul Meth
 14 , 235 , 1992
Cells
Epithelial
Enzymes
Medium
DMEM
Epithelial
Guinea-Pig
Guinea-pig, Hartley albino,
500-600 g
Chaminadas, G. , Alkhalaf, M. , Remy-Martin, J.P. , Propper, A.Y. and Adessi, G.L.
Specific Effect of Oestrone Sulphate on Protein Synthesis and Secretion by Cultured Epithelial Cells from Guinea-pig Endometrium ,
J Endocrinol
 123 , 233 , 1989
Cells
Endometrial
Enzymes
Collagenase : 0.25%
Medium
HBSS
Eye
Chicken
Chick, embryo, 6 day
Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G.
Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions ,
Cell Death Differ
 180 , 159-68 , 2005
Cells
Retinal cells
Enzymes
Trypsin : 0.005%
Medium
DMEM/F12
Eye
Chicken
Chick embryo
Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H.
ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent ,
Inv Ophthal Visual Sci
 43 , 3181-9 , 2002
Cells
Corneal epithelia
Enzymes
Collagenase : 0.08%
Trypsin : 0.08%
Medium
HBSS
Eye
Chicken
Chick embryo
(also rat)
Seigel, G.
The Golden Age of Retinal Cell Culture ,
Mol Vis
 5 , 4 , 1999
Cells
Retinal
Enzymes
Trypsin : 0.6%
Medium
DMEM
Eye
Chicken
Chick, embryo, 10-14 day
Moyer, M. , Bullrich, F. and Sheffield, J.
Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina ,
In Vitro Cell Dev Biol
 26 , 1073 , 1990
Cells
Flat, retina
Enzymes
Trypsin : 0.1%
Medium
Tyrode's solution, CMF
Heart
Canine
Canine
Zhang,D. , Wu, C. , Qi, X. , Meijering, R. , Hoogstra-Berends, F. , Tadevosyan, A. and Cubukcuoglu, et al.
Activation of Histone Deacetylase-6 Induces Contractile Dysfunction Through Derailment of alpha-tubulin Proteostasis in Experimental and Human Atrial Fibrillation. ,
Circulation
 129 , 346-58 , 2014
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
Tyrode's solution
Heart
Canine
Canine
Zhang, H. , Silva, J. , Lin, Y. , Verbsky, J. , Lee, U. , Kanter, E. , Yamada, K. , Schuessler, R. and Nichols, C.
Heterogeneity and Function of K(ATP) Channels in Canine Hearts. ,
Heart Rhythm
 10 , 1576-83 , 2013
Cells
Atrial and ventricular myocytes
Enzymes
Collagenase Type 2 : 0.05-0.08%
Medium
See reference
Heart
Canine
Canine, adult
Bonilla, I. , Sridhar, A. , Nishijima, Y. , Gyorke, S. , Cardounel, A. and Carnes, C.
Differential Effects of the Peroxynitrite Donor, SIN-1, on Atrial and Ventricular Myocyte Electrophysiology. ,
J Cardiovasc Pharmacol
 61 , 401-7 , 2013
Cells
Atrial and ventricular myocytes
Enzymes
Medium
See reference
Heart
Canine
Canine
Xi, Y. , Wu, G. , Ai, T. , Cheng, N. , Kalisnik, J. , Sun, J. , Abbasi, S. , Yang, D. , Fan, C. , Yuan, X. and Wang, S. et al.
Ionic Mechanisms Underlying the Effects of Vasoactive Intestinal Polypeptide on Canine Atrial Myocardium. ,
Circ Arrhythm Electrophysiol
 6 , 976-83 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 300 u/ml
Protease : 0.03%
Medium
Tyrode's solution

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.