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Tissue References Search Tool

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Pancreas
Canine
Canine
Zoran, M. , Doyle, R. and Haydon, P.
Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis ,
Neuron
 6 , 145 , 1991
Cells
Buccal ganglia; SLT muscle
Enzymes
Trypsin : 0.2%
Medium
DMEM
Pancreas
Canine
Canine, adult mongrel, 15-25 kg
Noel, J. , Rabinovitch, A. , Olson, L. , Kyriakides, G. , Miller, J. and Mintz, D.
A Method for Large-Scale, High-Yield Isolation of Canine Pancreatic Islets of Langerhans ,
Metabolism
 31 (2) , 184 , 1982
Cells
Islets
Enzymes
Collagenase Type 4 : 600-1100 u/ml
Deoxyribonuclease I : 10 ug/ml
Medium
RPMI 1640
Pancreas
Porcine
Porcine, juvenile
Van der Burg Michael P M, Graham John M.
Iodixanol Density Gradient Preparation in University of Wisconsin solution for porcine islet purification ,
ScientificWorldJournal
 3 , 1154-9 , 2003
Cells
Islets
Enzymes
Collagenase : see reference
Medium
Univ of Wisconsin solution
Pancreas
Porcine
Porcine, 3 month, 15-20 kg
Zhao, X. , Han, J. and Tang, C.
Primary Culture of Porcine Pancreatic Acinar Cells ,
JOP
 2 (2) , 78 , 2001
Cells
Acinar
Enzymes
Collagenase Type 3 : 200 u/ml
Medium
RPMI 1640
Pancreas
Porcine
Porcine, 1-3 year, 1.5-2.0 kg, either sex
Korbutt, G. , Elliott, J. , Ao, Z. , Smith, D. , Warnock, G. and Rajotte, R.
Large Scale Isolation, Growth, and Function of Porcine Neonatal Islet Cells ,
J Clin Invest
 97 (9) , 2119 , 1996
Cells
Islets
Enzymes
Collagenase : 0.25%
Medium
HBSS
Pancreas
Porcine
Porcine, female
Brandhorst, D. , Brandhorst, H. , Hering, B. , Federlin, K. and Bretzel, R.
Islet Isolation from the Pancreas of Large Mammals and Humans: 10 Years of Experience ,
Exp Clin Endocrinol
 103 , 3 , 1995
Cells
Islets
Enzymes
Collagenase : 0.1%
Medium
HBSS
Pancreas
Porcine
Porcine
Johnson, P. , van Suylichem, P. , Roberts, D. , Vos-Scheperkeuter, G. , White, S. , van Schlifgaarde, R. and London, N.
Design of a Simple, in vitro Method for Evaluation of the Efficiency of Crude Clostridium histolyticum Collagenase and its Components for Porcine Islet Isolation ,
Xenotransplantation
 2 , 165 , 1995
Cells
Islets
Enzymes
Collagenase : 0.1%-0.2%
Medium
HBSS
Pancreas
Porcine
Porcine, <2 year, 200-250 kg
Heiser, A. , Ulrichs, K. and Muller-Ruchholtz, W.
Isolation of Porcine Pancreatic Islets: Low Trypsin Activity During the Isolation Procedure Guarantees Reproducible High Islet Yields ,
J Clin Lab Anal
 8 , 407 , 1994
Cells
Islets
Enzymes
Collagenase : 0.1%
Medium
HBSS
Pancreas
Porcine
Porcine, 10-36 months, 200-300 kg
Ricordi, C. , Socci, C. , Davalli, A. , Staudacher, C. , Baro, P. , Vertova, A. , Sassi, I. , Gavazzi, F. , Pozza, G. and Di Carlo, V.
Isolation of the Elusive Pig Islet ,
Surgery
 107 (6) , 688 , 1990
Cells
Islets
Enzymes
Collagenase : 0.2%
Medium
HBSS
Pancreas
Porcine
Porcine
Iwatsuki, N. and Peterson, O.
Action of Tetraethylammonium on Calcium-Activated Potassim Channels in Pig Pancreatic Acinar Cells Studied by Patch-Clamp Single-Channel and Whole-Cell Current Recording ,
J Membr Biol
 86 , 139 , 1985
Cells
Acinar
Enzymes
Collagenase : 100 u/ml
Medium
Saline

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.