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Tissue References Search Tool

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Bone
Chicken
Chick, day old
Gay, C. , Lloyd, Q. and Gilman, V.
Characteristics and Culture of Osteoblasts Derived From Avian Long Bone ,
In Vitro Cell Dev Biol
 30A , 379 , 1994
Cells
Osteoblasts
Enzymes
Trypsin : 0.03%
Medium
DMEM
Bone
Chicken
Chick, Peterson/Arbor Acre, male, 4 weeks old (Gallus domesticus)
Rosselot, G. , Reginato, A.M. and Leach, R.M.
Development of a Serum-Free System to Study the Effect of Growth Hormone and Insulinlike Growth Factor-1 on Cultured Postembryonic Growth Plate Chondrocytes ,
In Vitro Cell Dev Biol
 28A , 235 , 1992
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Medium
Ham's F12
Bone
Chicken
Chick, embryo
Schiltz, J.R. , Mayne R. and Holtzer, H.
The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture ,
Differentiation
 1 , 97 , 1973
Cells
Vertebrae chondroblasts
Enzymes
Trypsin : 0.25%
Medium
Simm's, CMF
Muscle
Chicken
Chicken, 1-2 day
Dirksen W. , Vladic F. and Fisher S.
A Myosin Phosphatase Targeting Subunit Isoform Transition Defines a Smooth Muscle Developmental Phenotypic Switch ,
Am J Physiol/Cell
 278(3) , C589 , 2000
Cells
Gizzard and aorta smooth muscle
Enzymes
Medium
HBSS
Muscle
Chicken
Chick
Chamley-Campbell, J. , Campbell, G. and Ross, R.
The Smooth Muscle in Cell Culture ,
Physiol Res
 59 , 1 , 1979
Cells
Smooth muscle
Enzymes
Trypsin : 0.05% - 0.1%
Medium
HBSS
Muscle
Chicken
Chick, white leghorn, 12 day
Bullaro, J. and Brookman, D.
Comparison of Skeletal Muscle Monolayer Cultures Initiated With Cells Dissociated by the Vortex and Trypsin Methods ,
In Vitro
 12 , 564 , 1976
Cells
Muscle
Enzymes
Trypsin : 0.25%
Medium
Puck's saline A
Muscle
Chicken
Chick, white leghorn, embryos, 11 day
Tepperman, K. , Morris, G. , Essien, F. and Heywood, S.M.
A Mechanical Dissociation Method For Preparation of Muscle Cell Cultures ,
J Cell Physiol
 86 , 561 , 1975
Cells
Muscle
Enzymes
Trypsin : 0.05%
Medium
Saline G
Muscle
Chicken
Chick embryo
Hilfer, S. and Brown, J.
Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart ,
Exp Cell Res
 65 , 246 , 1971
Cells
Thyroid
Muscle
Heart
Enzymes
Collagenase : 0.25%
Medium
Tyrode's saline, potassium free
Muscle
Chicken
Chick, embryonic
Hilfer, S.
Collagenase Treatment of Chick Heart and Thyroid , Tissue Cult Methods & Applications , Kruse, P., and Patterson, M. , 246 , 1971
Cells
Muscle
Enzymes
Trypsin : 0.1%
Medium
CMF HBSS
Muscle
Chicken
Chick embryonic
Rinaldini, L.
An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells ,
Exp Cell Res
 16 , 477 , 1959
Cells
Various tissues (heart, liver, skeletal, cardiac)
Enzymes
Trypsin : various grades
Medium
CMF Tyrode's solution

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.