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Tissue References Search Tool

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Heart
Fish
Zebrafish
Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.
Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
 117 , 2687-2692 , 2020
Cells
Cardiac Myocytes
Enzymes
Trypsin : 0.08%
Protease XIV : 0.005%
Collagenase Type 1 : 0.1-0.2%
Medium
HEPES
Heart
Fish
Fish, Danio rerio
Sander, V. , Sune, G. , Jopling, C. , Morera, C. and Belmonte J.
Isolation and In Vitro Culture of Primary Cardiomyocytes from Adult Zebrafish Hearts. ,
Nat Protoc
 8 , 800-9 , 2013
Cells
Cardiomyocytes
Enzymes
Medium
MEM
Heart
Guinea-Pig
Guinea pig, male, 350-400g
Wang, Y. , Geng, J. , Jiang, M. , Li, C. , Han, Y. and Jiang, J.
The Cardiac Electrophysiology Effects of Higenamine in Guinea Pig Heart ,
Biomed Pharmacother
 109 , 2348 , 2019
Cells
Ventricular myocytes
Enzymes
Medium
Tyrodes solution
Heart
Guinea-Pig
Guinea pig, adult
Zorn-Pauly, K. , Schaffer, P. , Pelzmann, B. , Bernhart, E. , Lang, P. and Koidl, B.
L-type and T-type Ca2+ current in cultured ventricular guinea pig myocytes ,
Physiol Res
 53(4) , 369 , 2004
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 100 u/ml
Medium
M-199
Heart
Guinea-Pig
Guinea-Pig
Dhamoon, A, Pandit, S. , Sarmast, F. , Parisian, K. , Guha, P. , Li, Y. , Bagwe, S. , Taffet, S. and Anumonwo, J.
Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current. ,
Circ Res
 94 , 1332-9 , 2004
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 100-200 u/ml
Medium
See reference
Heart
Guinea-Pig
Guinea pig, female, 300-350g
Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J.
Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium ,
Am J Physiol Heart Circ Physiol
 281 , H1657-66 , 2001
Cells
Coronary endothelial
Enzymes
Medium
see reference
Heart
Guinea-Pig
Guinea pig, 250-450 g
Preisig-Muller, R. , Mederos Y Schnitzler, M. , Derst, C. and Daut J.
Separation of Cardiomyocytes and Coronary Endothelial Cells for Cell-Specific RT-PCR ,
Am J Physiol
 277 , H413 , 1999
Cells
Endothelial
Enzymes
Medium
Perfusing solution
(see reference)
Heart
Guinea-Pig
Guinea-pig, male, 450-600 g
Stemmer, P. , Akera,T. , Brody, T. , Rardon, D. and Watanabe, A.
Isolation and Enrichment of Ca2+-Tolerant Myocytes for Biochemical Experiments from Guinea-Pig Heart ,
Life Sci
 44 , 1231 , 1989
Cells
Myocytes
Enzymes
Hyaluronidase : 0.02%
Medium
Krebs Henseleit bicarbonate buffer
Heart
Guinea-Pig
Guinea-pig
Ishihara, K. , Mitsuiye, T. , Noma, A. and Takano, M.
The Mg2+ Block and Intrinsic Gating Underlying Inward Rectification of the K+ Current in Guinea-Pig Cardiac Myocytes ,
J Physiol
 419 , 297 , 1989
Cells
Cardiomyocytes
Enzymes
Collagenase : 0.04%
Medium
Tyrode solution, CF
Heart
Guinea-Pig
Guinea-pig
Bridge, J. , Spitzer, K. and Ershler, P.
Relaxation of Isolated Ventricular Cardiomyocytes by a Voltage-Dependent ,
Science
 241 , 823 , 1988
Cells
Myocytes
Enzymes
Hyaluronidase : 0.10%
Medium
Bicarbonate buffer, CF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.