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Tissue References Search Tool

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Adipose/Fat
Guinea-Pig
Guinea-pig, adult
(also rat, hamster, gerbil, rabbit, lamb)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Hamster
Hamster, adult
(also rat, gerbil, rabbit, lamb, guinea-pig)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Hamster
Hamster, 5 week-12 month
DiGirolamo, M. , Mendlinger, S. and Fertig, J.W.
A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species ,
Am J Physiol
 221 , 850 , 1971
Cells
White fat
Enzymes
Collagenase : 0.05%
Medium
Kreb's Ringer bicarbonate buffer
Adipose/Fat
Human
Human
Alstrup, T. , Eijken, M. , Bohn, A. , Moller, B. and Damsgaard, T.
Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat. ,
Curr Protoc Stem Cell Biol
 48 , e68 , 2019
Cells
Adipose derived stem
Enzymes
Collagenase Type 4 : 150-300 u/ml
Medium
RPMI-1640
Adipose/Fat
Human
Human
Tsurumachi, N. , Akita, D. , Kano, K. , Matsumoto, T. , Toriumi, T. , Kazama, T. , Oki, Y. , Saito-Tamura, Y. , Tonogi, M. , Shimizu, N. and Honda, M.
Effect of Collagenase Concentration on The Isolation of Small Adipocytes from Human Buccal Fat Pad. ,
J Oral Sci
 , 2018
Cells
Adipocytes
Enzymes
Collagenase Type 2 : 0.01-0.5%
Medium
DMEM
Adipose/Fat
Human
Human
Lin, Y. , Marin-Argany, M. , Dick, C. , Redhage, K. , Blancas-Mejia, L. , Bulur, P. , Butler, G. , Deeds, M. , Madden, B. , Williams, A. , Wall, J. , Dietz, A. and Ramirez-Alvarado, M.
Mesenchymal Stromal Cells Protect Human Cardiomyocytes from Amyloid Fibril Damage ,
Cytotherapy
 19 , 1426-1437 , 2017
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
Adipose/Fat
Human
Human
Clark, K. , Fierro, F. , Ko, E. , Walker, N. , Arzi, B. , Tepper, C. , Dahlenburg, H. , Cicchetto, A. , Koi, A. , Marsh, L. , Murphy, W. , Fazel, N. and Borjesson, D.
Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome. ,
Stem Cell Res Ther
 8 , 69 , 2017
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
PBS
Adipose/Fat
Human
Human
Wahl, S. , Drong, A. , Lehne, B. , Loh, M. , Scott, K. and Kunze, S. et al.
Epigenome-wide Association Study of Body Mass Index, and the Adverse Outcomes of Adiposity ,
Nature
 541 , 81 , 2017
Cells
Adipocytes
Enzymes
Medium
HBSS
Adipose/Fat
Human
Human
Duscher, D. , Maan, Z. , Luan, A. , Aitzetmuller, M. , Brett, E. , Atashroo, D. , Whittam, A. , Hu, M. , Walmsley, G. , Houschyar, K. , Schilling, A. , Machens, H. , Gurtner, G. , Longaker, M. and Wan, D.
Ultrasound-Assisted Liposuction Provides a Source for Functional Adipose-Derived Stromal Cells. ,
Cytotherapy
 19 , 1491-1500 , 2017
Cells
Stromal
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human
Munir, H. , Ward, L. , Sheriff, L. , Kemble, S. , Nayar, S. , Barone, F. , Nash, G. and McGettrick, H.
Adipogenic Differentiation of Mesenchymal Stem Cells Alters Their Immunomodulatory Properties in a Tissue-Specific Manner. ,
Stem Cells
 35 , 1636-1646 , 2017
Cells
Mesenchymal stem
Enzymes
Medium
DMEM/F-12
Adipose/Fat
Human
Human
Hu, J. , Cao, Y. , Xie, Y. , Wang, H. , Fan, Z. , Wang, J. , Zhang, C. , Wang, J. and Wu, C. aand Wang, S.
Periodontal Regeneration in Swine After Cell Injection and Cell Sheet Transplantation of Human Dental Pulp Stem Cells Following Good Manufacturing Practice. ,
Stem Cell Res Ther
 7 , 130 , 2016
Cells
Dental pulp stem
Enzymes
Medium
HBSS
Adipose/Fat
Human
Human
Szoke, K. , Reinisch, A. , Ostrup, E. , Reinholt, F. and Brinchmann, J.
Autologous Cell Sources in Therapeutic Vasculogenesis: In Vitro and In Vivo Comparison of Endothelial Colony-Forming Cells from Peripheral Blood and Endothelial Cells Isolated from Adipose Tissue. ,
Cytotherapy
 18 , 242-52 , 2016
Cells
Adipose derived endothelial
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human
Cheng, N. , Hsieh, T. , Lai, H. and Young, T.
High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells ,
Cytotherapy
 18 , 371-83 , 2016
Cells
Adipose stem
Enzymes
Medium
DMEM/F12
Adipose/Fat
Human
Human
Esteve, D. , Boulet, N. , Volat, F. , Zakaroff-Girard, A. , Ledoux, S. , Coupaye, M. , Decaunes, P. , Belles, C. , Gaits-Iacovoni, F. , Iacovoni, J. , Remaury, A. , Castel, B. , Ferrara, P. , Heymes, C. and Lafonta, M. Boloumie, J.
Human White and Brite Adipogenesis is Supported by MSCA1 and is impaired by Immune Cells. ,
Stem Cells
 33 , 1277-91 , 2015
Cells
Adipose stroma-vascular
Enzymes
Neutral Protease : 2.4 u/ml
Collagenase : 250 u/ml
Medium
PBS
Adipose/Fat
Human
Human
Satish, L. , Krill-Burger, J. , Gallo, P. , Etages, S. , Liu, F. , Philips, B. , Ravuri, S. , Marra, K. , LaFramboise, W. , Kathju, S. and Rubin, J.
Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage. ,
BMC Med Genomics
 8 , 41 , 2015
Cells
Adipose derived stem cells
Enzymes
Medium
HBSS
Adipose/Fat
Human
Human, female
Kinoshita, K. , Kuno, S. , Ishimine, H. , Aoi, N. , Mineda, K. , Kato, H. , Doi, K. , Kanayama, K. , Feng, J. , Mashiko, T. , Kurisaki, A. and Yoshimura, K.
Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers. ,
Stem Cells Transl Med
 4 , 146 , 2015
Cells
Adipose stromal
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human
Seaman, S. , Tannan, S. , Cao, Y. , Peirce, S. and Lin, K.
Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts. ,
Plast Reconstr Surg
 136 , 189e-199e , 2015
Cells
Adipocytes
Enzymes
Medium
PBS
Adipose/Fat
Human
Human
Choudhery,M, Badowski, M, Muise, A. and Harris, D
Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells. ,
Cytotherapy
 17 , 359-68 , 2015
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
Adipose/Fat
Human
Human
Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V. and De Kock, J
Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods. ,
Cytotherapy
 16 , 1220-8 , 2014
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
Adipose/Fat
Human
Human, adult
Koellensperger, E. , Bollinger, N. , Dexheimer, V. , Gramley, F. , Germann, G. and Leimer, U.
Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities. ,
Cytotherapy
 16 , 789 , 2014
Cells
Adipose derived stem
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human, adult
Al-Saqi, S. , Saliem, M. , Asikainen, S. , Quezada, H. , Ekblad, A. , Hovatta, O. , Le Blanc, K. , Jonasson, A. and Gotherstrom, C.
Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells. ,
Cytotherapy
 16 , 915 , 2014
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
see reference
Adipose/Fat
Human
Human
Doi, K. , Tanaka, S. , Iida, H. , Eto, H. , Kato, H. , Aoi, N. , Kuno, S. , Hirohi, T. and Yoshimura, K.
Stromal Vascular Fraction Isolated from Lipo-Aspirates Using an Automated Processing System: Bench and Bed Analysis. ,
J Tissue Eng Regen Med
 7 , 864 , 2013
Cells
Stromal vascular fraction
Enzymes
Medium
PBS
Adipose/Fat
Human
Human
Carvalho, P. , Gimble, J. , Dias, I. , Gomes, M. and Reis, R.
Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells. ,
Tiss Eng
 19 , 473-8 , 2013
Cells
Adipose stromal stem
Enzymes
Medium
DMEM/Hams F-12
Adipose/Fat
Human
Human
Cervelli, V. , Scioli, M. , Gentile, P. , Doldo, E. , Bonanno, E. , Spagnoli, L. and Orlandi, A.
Platelet-Rich Plasma Greatly Potentiates Insulin-Induced Adipogenic Differentiation of Human Adipose-Derived Stem Cells Through a Serine/Threonine Kinase Akt-dependent Mechanism and Promotes Clinical Fat Graft Maintenance. ,
Stem Cells Transl Med
 1 , 206-20 , 2012
Cells
Adipose-derived stem
Enzymes
Medium
PBS
Adipose/Fat
Human
Human
Gentile, P. , Orlandi, A. , Scioli, M. , Di Pasquali, C. , Bocchini, I. and Cervelli, V.
Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery. ,
Stem Cells Transl Med
 1 , 230-6 , 2012
Cells
Adipose derived stromal vascular
Enzymes
Medium
PBS
Adipose/Fat
Human
Human, adult
Hagman, D. , Kuzma, J. , Larson, I. , Foster-Schubert, K. , Kuan, L. , Cignarella, A. , Geamanu, E. , Makar, K. , Gottlieb, J. and Kratz, M.
Characterizing and Quantifying Leukocyte Populations in Human Adipose Tissue: Impact of Enzymatic Tissue Processing. ,
J Immunol Methods
 386 , 50 , 2012
Cells
Stromovascular
Enzymes
Medium
PBS with BSA
Adipose/Fat
Human
Human
Wu, I. , Nahas, Z. , Kimmerling, K. , Rosson, G. and Elisseeff, J.
An Injectable Adipose Matrix for Soft-Tissue Reconstruction. ,
Plast Reconstr Surg
 129 , 1247 , 2012
Cells
Adipose derived stem
Enzymes
Medium
DMEM/F-12
Adipose/Fat
Human
Human
Gentile, P. , Orlandi, A. , Scioli, M. , Di Pasquali, C. , Bocchini, I. , Curcio, C. , Floris, M. , Fiaschetti, V. , Floris, R. and Cervell, V.
A Comparative Translational Study: The Combined Use of Enhanced Stromal Vascular Fraction and Platelet-Rich Plasma Improves Fat Grafting Maintenance in Breast Reconstruction. ,
Stem Cells Transl Med
 1 , 341-51 , 2012
Cells
Stromal vascular, adipocytes
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human
Naaijkens, B. , Niessen, H. , Prins, H. , Krijnen, P. , Kokhuis, T. , de Jong, N,, van Hinsbergh, V. , Kamp, O. , Helder, M. , Musters, R. , van Dijk, A. and Juffermans, L.
Human Platelet Lysate as a Fetal Bovine Serum Substitute Improves Human Adipose-Derived Stromal Cell Culture for Future Cardiac Repair Applications. ,
Cell Tissue Res
 348 , 119 , 2012
Cells
Adipose derived stromal
Enzymes
Medium
PBS
Adipose/Fat
Human
Human
James, A. , Zara, J. , Corselli, M. , Askarinam, A. , Zhou, A. , Hourfar, A. , Nguyen, A. , Megerdichian, S. , Asatrian, G. , Pang, S. , Stoker, D. , Zhang, X. , Wu, B. , Ting, K. , Peault, B. and Soo, C.
An Abundant Perivascular Source of Stem Cells for Bone Tissue Engineering. ,
Stem Cells Transl Med
 1 , 673 , 2012
Cells
Perivascular stem
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human, male 40-60 years
Blasi, A. , Martino, C. , Balducci, L. , Saldarelli, M. , Soleti, A. , Navone, S. , Canzi, L. , Cristini, S. , Invernici, G. , Parati, E. and Alessandri, G.
Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential ,
Vasc Cell
 3 , 5 , 2011
Cells
Adipose derived stem cells
Enzymes
Collagenase : 0.25%
Medium
PBS
Adipose/Fat
Human
Human
Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J.
Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. ,
Lipids Health Dis
 10 , 171 , 2011
Cells
Renal adipose derived cells
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human
Yang, X. , He, X. , He, J. , Zhang, L. , Su, X. , Dong, Z. , Xu, Y. , Li, Y. and Li, YL.
High Efficient Isolation and Systematic Identification of Human Adipose-Derived Mesenchymal Stem Cells. ,
J Biomed Sci
 18 , 59 , 2011
Cells
Adipose derived mesenchymal
Enzymes
Medium
L-DMEM
Adipose/Fat
Human
Human
Yu, G. Floyd, ZE. , Wu, X. , Halvorsen, Y. and Gimble, J.
Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates. ,
Methods Mol Biol
 702 , 17-27 , 2011
Cells
Adipose derived stem
Enzymes
Medium
DMEM/Ham's F-12
Adipose/Fat
Human
Human
Basu, S. , Haghiac, M. , Surace, P. , Challier, J. , Guerre-Millo, M. , Singh, K. , Waters, T. , Minium, J. , Presley, L. , Catalano, P. and Hauguel-de Mouzon, S.
Pregravid Obesity Associates with Increased Maternal Endotoxemia and Metabolic Inflammation. ,
Obesity
 19 , 476 , 2011
Cells
Adipocytes
Enzymes
Medium
RPMI
Adipose/Fat
Human
Human
Peters, R. , Wolf, M. , van den Broek, M. , Nuvolone, M. , Dannenmann, S. , Stieger, B. , Rapold, R. , Konrad, D. , Rubin, A. , Bertino, J. , Aguzzi, A. , Heikenwalder, M. and Knuth, A.
Efficient Generation of Multipotent Mesenchymal Stem Cells from Umbilical Cord Blood in Stroma-Free Liquid Culture. ,
PLoS ONE
 5 , e15689 , 2010
Cells
Adipocytes
Enzymes
Medium
KRB

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.