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Tissue References Search Tool

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Kidney
Rat
Rat, SD, male
Eitle E, Hiranyachattada S, Wang H, Harris PJ.
Inhibition of proximal tubular fluid absorption by nitric oxide and atrial natriuretic peptide in rat kidney ,
Am J Physiol
 274 , C1075-80 , 1998
Cells
Proximal tubule suspensions
Enzymes
Pronase E : 2 u/ml
Medium
HEPES buffer
Kidney
Rat
Rat, SD, male
Schafer, J. , Watkins, M. , Li, L. , Herter, P. , Haxelmans, S. and Schlatter, E.
A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments ,
Am J Physiol
 273 , F650 , 1997
Cells
Nephron segments
Enzymes
Medium
Eagle's MEM
Kidney
Rat
Rat, 12-weeks-old
Ishikawa, S. , Kusaka, I. , Higashiyama, M. , Nagasaka, S. , Saito, T. , Honda, K. and Saito, T.
Cellular Signaling and Proliferative Action of AVP in Mesangium of SHR: Effect of Low Density Lipoprotein ,
Nat Neurosci
 50 , 1506 , 1996
Cells
Renal
Enzymes
Collagenase : 0.1%
Medium
DMEM
Kidney
Rat
Rat, male, 150-200 g
Elliget, K. and Trump, B.
Primary Cultures of Normal Rat Kidney Proximal Tubule Epithelial Cells for Studies of Renal Cell Injury ,
In Vitro Cell Dev Biol
 27 , 739 , 1991
Cells
Epithelial
Proximal tubule
Enzymes
Protease : 0.1%
Medium
HBSS/CMF
Kidney
Rat
Rat, adult
Brion, L. , Schwartz, J. , Lachman, H. , Zavilowitz, B. and Schwartz, G.
Development of H+ Secretion by Cultured Renal Inner Medullary Collecting Duct Cells ,
Am J Physiol
 257 , F486 , 1989
Cells
Inner medullary collecting duct
Papillae
Enzymes
Medium
PBS
Kidney
Rat
Rat, Wistar, female, 100-150 g
Wang, J. , Kester, M. and Dunn, M.
The Effects of Endotoxin on Platelet-Activating Factor Synthesis in Cultured Rat Glomerular Mesangila Cells ,
Biochim Biophys Acta
 969 , 217 , 1988
Cells
Glomerular mesangial
Enzymes
Collagenase : 0.025%
Medium
RPMI 1640
Kidney
Rat
Rat, Wistar, male, 150 - 200 g
Barlet-Bas, C. , Khadouri, C. , Marsey, S. and Doucet, A.
Sodium-Independent In Vitro Induction of Na+,K+-ATPase by Aldosterone in Renal Target Cells: Permissive Effect of Triiodothyronine ,
Proc Natl Acad Sci U S A
 85 , 1707 , 1988
Cells
Renal target
Enzymes
Collagenase : 1.0% (also 0.7%)
Medium
Eagle's MEM
Kidney
Rat
Rat, Wistar, male, adult
Gesek, F. , Wolff, D. and Strandhov, J.
Improved Separation Method for Rat Proximal and Distal Renal Tubules ,
Am J Physiol
 253 , F358 , 1987
Cells
Proximal tubules
Enzymes
Collagenase : 0.2%
Medium
Krebs-Henseleit buffer
Kidney
Rat
Rat,Wistar, 300-400 gm
Vinay, P. , Gougoux, A. and Lemieux, G.
Isolation of a Pure Suspension of Rat Proximal Tubules ,
Am J Physiol
 241 , F403 , 1981
Cells
Proximal tubules
Enzymes
Collagenase : 0.15 %
Medium
Krebs Henseleit solution
Kidney
Rat
Rat
Wallach, D. , Anderson, W. and Pastan, I.
Activation of Adenylate Cyclase in Cultured Fibroblasts by Trypsin ,
J Biol Chem
 253 , 24 , 1978
Cells
Fibroblasts
Kidney
Enzymes
Trypsin : up to 0.25%
Medium
Dulbecco-Vogt MEM
Liver
Rat

Rat, 8-14 week

Ge, J. , Zheng, Y. , Tsuchida, T. , Furuya, K. , Isoda, H. , Taniguchi, H. , Ohkohchi, N. and Oda, T.
Hepatic Stellate Cells Contribute to Liver Regeneration through Galectins in Hepatic Stem Cell Nliche. ,
Stem Cell Res Ther
 11 , 425 , 2020
Cells
Parenchymal and non-parenchymal
Enzymes
Collagenase : 0.05%
Medium
Williams E
Liver
Rat

Wistar, male, 200-250 g

Berardo, C. , Ferrigno, A. , Siciliano, V. , Richelmi, P. , Vairetti, M. and Pasqua, L.
Isolation of Rat Hepatocytes for Pharmacological Studies on Metabotropic Glutamate Receptor (mGluR) Subtype 5: A Comparison Between Collagenase I Versus Collagenase IV. ,
Eur J Histochem
 64 , 2020
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Rat
Rat, SD, 500-700g
Perepelyuk, M. , Terajima, M. , Wang, A. , Georges, P. , Janmey, P. , Yamauchi, M. and Wells, R.
Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury. ,
Am J Physiol Gastrointest Liver Physiol
 304 , G605 , 2013
Cells
Hepatocytes and sinusoidal endothelial
Enzymes
Medium
HBSS
Liver
Rat
Rat, adult
Shen, L. , Hillebrand, A. , Wang, D. and Liu, M.
Isolation and Primary Culture of Rat Hepatic Cells. ,
J Vis Exp
 64 , e3917 , 2012
Cells
Hepatocytes
Enzymes
Medium
William's
Liver
Rat
Rat, adult
Wen, J. , Olsen, A. , Perepelyuk, M. and Wells, R.
Isolation of Rat Portal Fibroblasts by In Situ Liver Perfusion. ,
J Vis Exp
 64 , e3669 , 2012
Cells
Portal fibroblasts
Enzymes
Medium
DMEM/F-12
Liver
Rat
Rat
Budick-Harmelin, N. , Anavi, S. , Madar, Z. and Tirosh, O.
Fatty Acids-Stress Attenuates Gluconeogenesis Induction and Glucose Production in Primary Hepatocytes. ,
Lipids Health Dis
 11 , 66 , 2012
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Rat
Rat, SD, 240-320g
Pillai, V. and Mehvar, R.
Inhibition of NADPH-Cytochrome P450 Reductase by Tannic Acid in Rat Liver Microsomes and Primary Hepatocytes: Methodological Artifacts and Application to Ischemia-Reperfusion Injury ,
J Pharm Sci
 100 , 3495 , 2011
Cells
Hepatocytes
Enzymes
HIS kit : per instructions
Medium
Waymouth's MB
Liver
Rat
Rat
Gopalakrishnan, S. and Harris, E.
In Vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion. ,
J Vis Exp
 57 , e3138 , 2011
Cells
Hepatocytes and SEC's
Enzymes
Collagenase Type 1 : 0.1-0.2%
Medium
RPMI
Liver
Rat
Rat, SD, 250-300g
Parasrampuria, R. and Mehvar, R.
Dose-Dependent Inhibition of Transporter-Mediated Hepatic Uptake and Biliary Excretion of Methotrexate by Cyclosporine A in an Isolated Perfused Rat Liver Model. ,
J Pharm Sci
 99 , 5060 , 2010
Cells
Hepatocytes
Enzymes
HIS kit : per instructions
Medium
Krebs-Henseleit
Liver
Rat
Rat, SD
Handy, J. , Saxena, N. , Fu, P. , Lin, S. , Mells, J. , Gupta, N. and Anania, F.
Adiponectin Activation of AMPK Disrupts Leptin-Mediated Hepatic Fibrosis via Suppressors of Cytokine Signaling (SOCS-3). ,
J Cell Biochem
 110 , 1195 , 2010
Cells
Hepatic stellate cells
Enzymes
Pronase : 0.02%
Collagenase : see reference
Medium
Medium 199
Liver
Rat
Rat, Wistar, female
Zvibel, I. , Atias, D. , Phillips, A. , Halpern, Z. and Oren, R.
Thyroid Hormones Induce Activation of Rat Hepatic Stellate Cells Through Increased Expression of p75 Neurotrophin Receptor and Direct Activation of Rho. ,
Lab Invest
 90 , 674 , 2010
Cells
Hepatic stellate cells
Enzymes
Collagenase Type 1 : 0.025-0.1%
Pronase : 0.025-0.13%
Medium
DMEM
Liver
Rat
Rat, SD
Chung, C. , Shugrue, C. , Nagar, A. , Doll, J. , Cornwell, M. , Gattu, A. , Kolodecik, T. , Pandol, S. and Gorelick, F.
Ethanol Exposure Depletes Hepatic Pigment Epithelium-Derived Factor, a Novel Lipid Regulator. ,
Gastroenterol
 136 , 331 , 2009
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HBSS
Liver
Rat
Rat, Lewis, male
Bettinger, C. , Kulig, K. , Vacanti, J. , Langer, R. and Borenstein, J.
Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture. ,
Tissue Eng Part A
 15 , 1321 , 2009
Cells
Hepatocytes
Enzymes
Medium
DPBS
Liver
Rat
Rat, 200-300 g
Mula, N. , Cubero, F. , Codesal, J. , de Andres, S. , Escudero, C. , Garcia-Barrutia, S. , Millan, I. , Arahuetes, R. and Maganto, P.
Survival of Allogeneic Hepatocytes Transplanted into the Thymus. ,
Cells Tissues Organs
 188 , 270 , 2008
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Rat
Rat, SD, 500-750 g
Li, Z. , Dranoff, J. , Chan, E. , Uemura, M. and Sevigny, J.and Wells, R.
Transforming Growth Factor-beta and Substrate Stiffness Regulate Portal Fibroblast Activation in Culture. ,
Hepatol
 46 , 1246-56 , 2007
Cells
Portal fibroblasts
Enzymes
Collagenase : 0.03%
Pronase : 0.033%
Hyaluronidase : 0.036%
Medium
DMEM/F-12
Liver
Rat
Rat, SD, 180-200g
Charbonneau, A. , Unson, C. and Lavoie, J.
High-Fat Diet-Induced Hepatic Steatosis Reduces Glucagon Receptor Content in Rat Hepatocytes: Potential Interaction with Acute Exercise. ,
J Physiol
 579 , 255 , 2007
Cells
Hepatocytes
Enzymes
Medium
see reference
Liver
Rat
Rat, SD, male, 230-250g
Doleh, L. and Romani, A.
Biphasic Effect of Extra-Reticular Mg2+ on Hepatic G6P Transport and Hydrolysis. ,
Arch Biochem Biophys
 467 , 283 , 2007
Cells
Hepatocytes
Enzymes
Medium
HEPES
Liver
Rat
Rat, Lewis, 150-200g
Smith, M. and Mooney, D.
Hypoxia Leads to Necrotic Hepatocyte Death. ,
J Biomed Mater Res A
 80 , 520 , 2007
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Rat
Rat, Wistar, 270-330g
Li, W. , Ralphs, K. , Slack, J. and Tosh, D.
Keratinocyte Serum-Free Medium Maintains Long-Term Liver Gene Expression and Function in Cultured Rat Hepatocytes by Preventing the Loss of Liver-Enriched Transcription Factors. ,
Int J Biochem Cell Biol
 39 , 541-54 , 2007
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Rat
Rat, Fisher, E14
Isabel Zvibel, Miri Bronstein, Einav Hubel, Ella Bar-Lev, Zamir Halpern, Ran Oren.
Isolation, characterization and culture of Thy1-positive cells from fetal rat livers ,
World J Gastroenterol
 12 , 3841-7 , 2006
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Rat
Rat, Lewis, female, 150-200 g
Sosef MN, Baust JM, Sugimachi K, Fowler A, Tompkins RG. and Toner M.
Cryopreservation of isolated primary rat hepatocytes: enhanced survival and long-term hepatospecific function ,
Ann Surg
 241 , 125 , 2005
Cells
Hepatocytes
Enzymes
Collagenase : see references
Medium
DMEM
Liver
Rat
Rat
Benten Daniel, Follenzi Antonia, Bhargava Kuldeep K, Kumaran Vinay, Palestro Christopher J, Gupta Sanjeev.
Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice ,
Hepatol
 42 , 140-8 , 2005
Cells
Liver sinusodial endothelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Liver
Rat
Rat, Wistar, 200-300 g
Putz G, Schmider W, Nitschke R, Kurz G, Blum HE.
Synthesis of phospholipid-conjugated bile salts and interaction of bile salt-coated liposomes with cultured hepatocytes ,
J Lipid Res
 46 , 2325-38 , 2005
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Rat
Rat, SD, male, 200-250 g
Raman Priya, Donkin Shawn S, Spurlock Michael E.
Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures ,
Am J Physiol Regul Integr Comp Physiol
 286 , R206-16 , 2004
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
DMEM
Liver
Rat
Rat, adult
Jensen C. , Jauho E. , Santoni-Rugiu E. , Holmskov U. , Teisner B. , Tygstrup N. and Bisgaard H.
Transit-amplifying Ductular (oval) Cells and Their Hepatocytic Progeny are Characterized by a Novel and Distinctive Expression of Delta-like Protein/preadipocyte Factor 1/fetal Antigen 1 ,
Am J Pathol
 164(4) , 1347 , 2004
Cells
Hepatocytes
Enzymes
Pronase : 1%
Medium
Williams E
Liver
Rat
Rat, Wistar
Wang Y. , Liu H. , Guo H. , Wen H. and Liu J.
Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich ,
World J Gastroenterol
 10 , 699 , 2004
Cells
Hepatocytes
Enzymes
Medium
RPMI 1640

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.