For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Bone
Human

Human

Klauzen, P. , Semenova, D. , Kostina, D. , Uspenskiy, V. and Malashicheva, A.
Purinergic Signaling in Pathologic Osteogenic Differentiation of Aortic Valve Interstitial Cells from Patients with Aortic Valve Calcification. , Biomedicines Vol. 11 , 2023
Cells
Valve interstitial and endothelial
HUVEC
Osteoblast
Enzymes
Medium
ECM
Bone
Human
Human
Agarwal, S. , Loder, S. , Sorkin, M. , Li, S. , Shrestha, S. , Zhao, B. , Mishina, Y. , James, A. and Levi, B.
Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. ,
Stem Cells
 34 , 1692-701 , 2016
Cells
Bone-cartilage-stromal
Enzymes
Medium
HBSS
Bone
Human
Human
Kode, A.,Manavalan1, J.,Mosialou1, I. , Bhagat G. and Rathinam, C, Luo, N et al.
Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts ,
Nature
 506 , 240 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Bone
Human
Human
Taylor, S. , Shah, M. and Orriss, I.
Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
 3 , 585 , 2014
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%-1.0%
Medium
DMEM
Bone
Human
Human, 22-73 year
Kneser U, Voogd A, Ohnolz J, Buettner O, Stangenberg L, Zhang YH, Stark GB, Schaefer DJ.
Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute ,
Cells Tissues Organs
 179 , 158-69 , 2005
Cells
Osteoblasts
Enzymes
Trypsin : 0.5%
Medium
Basal Medium
Bone
Human
Human, 60+ year
Chen X. , Qian H. , Neff L. , Satomura K. and Horowitz M.
Thy-1 antigen Expression by Cells in the Osteoblast Lineage ,
J Bone Miner Res
 14 , 362 , 1999
Cells
Bone Cells, Osteoblasts
Enzymes
Collagenase Type 2 : 200-250 u/ml
Medium
DMEM
Bone
Human
Human
Meikle, M. , Boyd, S. , Hembry, R. , Compston, J. , Croucher, P. and Reynolds, J.
Human Osteoblasts in Culture Synthesize Collagenase and Other Matrix Metalloproteinases in Response to Osteotropic Hormones and Cytokines ,
J Cell Sci
 103 , 1093 , 1992
Cells
Osteoblasts
Enzymes
Trypsin : 0.1%
Medium
DMEM
Bone
Human
Human
Fedarko, N.S. , Termine, J.D. , Young, M.F. and Robey, P.G.
Temporal Regulation of Hyaluronan and Proteoglycan Metabolism by Human Bone Cells in Vitro ,
J Biol Chem
 265 , 12200 , 1990
Cells
Osteoblasts
Enzymes
Collagenase Type 4 : 250 u/ml
Medium
DMEM
Bone
Rat
Rat, 2 day
Jeon, J. , Lee, M. and Yang, H.
Differentiated Osteoblasts Derived Decellularized Extracellular Matrix to Promote Osteogenic Differentiation. ,
Biomater Res
 22 , 4 , 2018
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Bone
Rat
Rat, Wistar, 250g
Jacobs, F. , Gijsen, H. , Van de Vyver, M. and Ferris, W.
Vanadate Impedes Adipogenesis in Mesenchymal Stem Cells Derived from Different Depots within Bone. ,
Front Endocrinol
 7 , 108 , 2016
Cells
Proximal femur stem
Enzymes
Medium
HBSS
Bone
Rat
Rat, 2-3 day
Taylor, S. , Shah, M. and Orriss, I.
Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
 3 , 585 , 2014
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%-1.0%
Medium
DMEM
Bone
Rat
Rat, fetal, 21 days of gestation
Owen, T. , Aronow, M. , Shalhoub, V. , Barone, L. , Wilming, L. , Tassinari, M. , Kennedy, M. , Pockwinse, S. , Lian, J. and Stein, G.
Progressive Development of the Rat Oseoblast Phenotype In Vitro: Reciprocal Relationships in Expression of Genes Associated with Osteoblast Proliferation and Differentiation During Formation of the Bone Extracellular Matrix ,
J Cell Physiol
 143 , 420 , 1990
Cells
Calvaria
Enzymes
Collagenase : 0.2%
Medium
MEM
Bone
Rat
Rat, newborn
Ernst, M. and Froesch, E.
Osteoblastlike Cells in a Serum-Free Methylcellulose Medium Form Colonies: Effects of Insulin and Insulinlike Growth Factor I ,
Calcif Tissue Int
 40 , 27 , 1987
Cells
Osteoblastlike cells
Enzymes
Medium
MEM
Bone
Rat
Rat, fetus, 17-21 day
Peck, W. , Birge, S. and Fedak, S.
Bone Cells: Biochemical and Biological Studies After Enzymatic Isolation ,
Science
 146 , 1476 , 1964
Cells
Calvaria
Enzymes
Collagenase : 0.01%-0.6%
Medium
Tris-buffered saline

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.