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Tissue References Search Tool

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Mammary
Human
Human
Labarge, M. , Garbe, J. and Stampfer, M.
Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture. ,
J Vis Exp
 71 , 2013
Cells
Mammary epithelial
Enzymes
Collagenase : 200 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM/F-12
Mammary
Human
Human, normal biopsy
Ronnov-Jessen L. , Villadsen R. , Edwards J. and Petersen O.
Differential Expression of a Chloride Intracellular Channel Gene, CLIC4, in Transforming Growth Factor-beta1-mediated Conversion of Fibroblasts to Myofibroblasts ,
Am J Pathol
 161 , 471 , 2002
Cells
Fibroblasts
Enzymes
Collagenase Type 3 : 900 u/ml
Medium
DMEM/F-12
Mammary
Human
Human
Huss, F. and Kratz, G.
Mammary Epithelial Cell and Adipocyte Co-Culture in a 3-D Matrix: The First Step Towards Tissue-Engineered Human Breast Tissue. ,
Cells Tissues Organs
 169 , 361-7 , 2001
Cells
Mammary epithelial cells
Enzymes
Medium
DMEM
Mammary
Human
Human
Ogmundsdottir, H. , Petursdottir, I. , Gudmundsdottir, I. , Amundadottir, L. , Ronnov-Jessen, L. and Petersen, O.
Effects of Lymphocytes and Fibroblasts on the Growth of Human Mammary Carcinoma Cells Studied in Short-Term Primary Cultures ,
In Vitro Cell Dev Biol
 29A , 936 , 1993
Cells
Epithelial
Fibroblasts
Enzymes
Collagenase Type 1 : 450 IU/ml
Medium
DMEM/Ham's F-12
Mammary
Human
Human
Ronnov-Jessen, L. , VanDeurs, B. , Nielsen, M. and Petersen, O.W.
Identification, Paracrine Generation, and Possible Function of Human Breast Carcinoma Myofibroblasts in Culture ,
In Vitro Cell Dev Biol
 28 , 273 , 1992
Cells
Myofibroblasts
Enzymes
Collagenase : 900 IU/ml
Medium
DME - F12
Mammary
Human
Human, female, 15-61 yrs old
Berthon, P. , Pancino, G. , Cremoux, P. , Roseto, A. , Gespach, C. and Calvo, F.
Characterization of Normal Breast Epithelial Cells in Primary Cultures: Differentiation and Growth Factor Receptors Studies ,
J Tiss Cul Meth
 28A , 716 , 1992
Cells
Epithelial
Enzymes
Hyaluronidase : 150 IU/ml
Medium
DMEM
Mammary
Human
Human
Emerman, J. and Wilkinson, D.
Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples ,
In Vitro Cell Dev Biol
 26 , 1186 , 1990
Cells
Epithelial
Enzymes
Collagenase : 2.0%
Medium
DMEM/Ham's F-12
Mammary
Human
Human
Leung, C. and Shiu, R.
Morphological and Proliferative Characteristics of Human Breast Tumor Cells Cultured on Plastic and in Collagen Matrix ,
In Vitro
 18 , 476 , 1981
Cells
Tumor, breast
Enzymes
Neuraminidase : 0.8 u/ml
Medium
HBSS
Mammary
Human
Human
Stampfer, M. , Hallowes, R. and Hackett, A.
Growth of Normal Human Mammary Cells in Culture ,
In Vitro
 16 (5) , 415 , 1980
Cells
Epithelial
Enzymes
Hyaluronidase : 100 u/ml
Medium
DMEM/Ham's F-12
Mammary
Rat
Rat
Mei, N. , McDaniel, L. , Dobrovolsky, V. , Guo, X. , Shaddock, J. , Mittelstaedt, R. , Azuma, M. , Shelton, S. , McGarrity, L. , Doerge, D. and Heflich, R.
The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats. ,
Toxicol Sci
 115 , 412 , 2010
Cells
Mammary epithelial
Enzymes
Medium
HBSS
Mammary
Rat
Rat, female, 50 day
Maffini M. , Soto A. , Calabro J. , Ucci A. and Sonnenschein C.
The Stroma as a Crucial Target in Rat Mammary Gland Carcinogenesis ,
J Cell Sci
 117 , 1495 , 2004
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F12
Mammary
Rat
Rat, female
Djuric Z. , Lewis S. , Mayhugh M. , Naegeli L. , Tang N. and Hart R.
Effect of Varying Caloric Restriction Levels on Female Rat Growth and 5-hydroxymethyl-2'-deoxyuridine in DNA ,
Toxicol Sci
 66 , 125 , 2002
Cells
Mammary gland epithelial
Enzymes
Medium
see reference
Mammary
Rat
Rat, SD
Brake P. , Zhang L. and Jefcoate C.
Aryl Hydrocarbon Receptor Regulation of Cytochrome P4501B1 in Rat Mammary Fibroblasts: Evidence for Transcriptional Repression by Glucocorticoids ,
Mol Pharmacol
 54 , 825 , 1998
Cells
Mammary fibroblasts
Enzymes
Medium
DMEM/F-12
Mammary
Rat
Rat, SD, 50 day
Varela L. , Darcy K. and Ip M.
The Epidermal Growth Factor Receptor is not Required for Tumor Necrosis Factor-alpha Action in Normal Mammary Epithelial Cells ,
Endocrinology
 138 , 3891 , 1997
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F12
Mammary
Rat
Rat
(also human)
Soriano, J. , Pepper, M. , Nakamura, T. , Orci, L. and Montesano, R.
Hepatocyte Growth Factor Stimulates Extensive Development of Branching Duct-like Structures by Cloned Mammary Gland Epithelial Cells ,
J Cell Sci
 108 , 413-430 , 1995
Cells
Epithelial
Enzymes
Medium
DMEM
Mammary
Rat
Rat, SD, female, 60-90 day
Laduca, J. and Sinha, D.
In Vitro Carcinogenesis of Mammary Epithelial Cells by N-Nitroso-N-Methylurea Using a Collagen Gel Matrix Culture ,
In Vitro Cell Dev Biol
 29A , 789 , 1993
Cells
Epithelial
Enzymes
Medium
EBSS
Mammary
Rat
Rat, Lewis, female, 90 days
Lin, T. , Hom, Y.K. , Richards, J. and Nandi, S.
Effects of Antioxidants and Reduced Oxygen Tension on Rat Mammary Epithelial Cells in Culture ,
In Vitro Cell Dev Biol
 27A , 191 , 1991
Cells
Epithelial
Mammary
Enzymes
Collagenase : 0.05%
Medium
Medium 199
Mammary
Rat
Rat, SD, female, 55 day
Ehmann, U. , Osborn, R. , Guzman, R. and Fajardo, L.
Cultured Proliferating Rat Mammary Epithelial Cells ,
In Vitro Cell Dev Biol
 27 , 749 , 1991
Cells
Epithelial
Enzymes
Neutral Protease : 3 u/ml
Medium
Medium 199
Mammary
Rat
Rat, 50-60 days
Hahm, H.A. and Ip, M.M.
Primary Culture of Normal Rat Mammary Epithelial Cells Within a Basement Matrix. 1. Regulation of Proliferation by Hormones and Growth Factors ,
In Vitro Cell Dev Biol
 26 , 791 , 1990
Cells
Epithelial
Enzymes
Medium
EBSS
Mammary
Rat
Rat, Fisher 344, virgin, 80-100 day
McGrath, M. , Palmer, S. and Nandi, S.
Differential Response of Normal Rat Mammary Epithelial Cells to Mammogenic Hormones and EGF ,
J Cell Physiol
 125 , 182 , 1985
Cells
Epithelial
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Mammary
Rat
Rat, LEW, virgin, female, 45-50 day
Ethler, S.
Primary Culture and Serial Passage of Normal and Carcinogen-Treated Rat Mammary Epithelial Cells In Vitro ,
J Natl Cancer Inst
 74 (6) , 1307 , 1985
Cells
Epithelial
Enzymes
Collagenase : 0.1%
Medium
Medium 199
Mammary
Rat
Rat, SD, female
Cohen, L.
Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma ,
In Vitro
 18 , 565 , 1982
Cells
Epithelial, cancer and tumor
Enzymes
Collagenase : 0.1%
Medium
Eagles's MEM
Mammary
Rat
Rat, inbred LEW, female, 50-60 day old
Richards, J. and Nandi, S.
Primary Culture of Rat Mammary Epithelial Cells. I. Effect of Plating Density, Horomones, and Serum on DNA Synthesis ,
J Natl Cancer Inst
 61 (3) , 765 , 1978
Cells
Epithelial
Enzymes
Medium
Medium 199
Mammary
Rat
Rat, Wistar, 13-18 day postpartum
Katz, J. , Wals, P. and Van de Velde, R
Lipogenesis by Acini from Mammary Gland of Lactating Rats ,
J Biol Chem
 249 , 7348 , 1974
Cells
Acini
Enzymes
Collagenase : 0.05%
Medium
HBSS
Mammary
Rat
Rat, SD, 15-20 day postpartum
Martin, R. and Baldwin, R.
Effects of Insulin on Isolated Rat Mammary Cell Metabolism: Glucose Utilization and Metabolite Patterns ,
Endocrinology
 89 , 1263 , 1971
Cells
Mammary
Enzymes
Collagenase : 0.2%
Medium
Kreb's Ringer bicarbonate buffer
Mammary
Rat
Rat, virgin
Moon, R. , Janns, D. and Young, S.
Preparation of Fat Cell-"Free" Rat Mammary Gland ,
J Histochem Cytochem
 17 (3) , 182 , 1969
Cells
Mammary
Enzymes
Collagenase : 0.35%
Medium
Medium 199

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.