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Tissue References Search Tool

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Cartilage
Human
Human
Andrews, S. , Kunze, M. , Mulet-Sierra, A. , Williams, L. , Ansari, K. , Osswald, M. and Adesida, A.
Strategies to Mitigate Variability in Engineering Human Nasal Cartilage. ,
Sci Rep
 7 , 6490 , 2017
Cells
Chondrocytes
Enzymes
Medium
DMEM
Cartilage
Human
Human
Muraglia, A. , Nguyen, V. , Nardini, M. , Mogni, M. , Coviello, D. , Dozin, B. , Strada, P. , Baldelli, I. , Formica, M. , Cancedda, R. and Mastrogiacomo, M.
Culture Medium Supplements Derived from Human Platelet and Plasma: Cell Commitment and Proliferation Support. ,
Front Bioeng Biotechnol
 5 , 66 , 2017
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Hyaluronidase : 0.1%
Collagenase Type 2 : 1000 u/ml
Collagenase Type 1 : 400 u/ml
Medium
Ham's F12
Cartilage
Human
Human
Tamai, M. , Nagasao, T. , Yanaga, H. , Hamamoto, Y. , Kogure, T. and Tanaka, Y.
Correction of Secondary Deformity after Nuss Procedure for Pectus Excavatum by Means of Cultured autologous Cartilage Cell Injection. ,
Int J Surg Case Rep
 15 , 70-3 , 2015
Cells
Chondrocytes
Enzymes
Collagenase : 0.3%
Medium
DMEM
Cartilage
Human
Human
Capsoni, F. , Ongari, A. , Lonati, C. , Accetta, R. , Gatti, S. and Catania, A.
Alpha-Melanocyte-Stimulating-Hormone (a-MSH) Modulates Human Chondrocyte Activation Induced by Proinflammatory Cytokines. ,
BMC Musculoskelet Disord
 16 , 154 , 2015
Cells
Chondrocytes
Enzymes
Medium
DMEM
Cartilage
Human
Human, 52-82 years
Pallu, S. , Francin, P. , Guillaume, C. , Gegout-Pottie, P. , Netter, P. , Mainard, D. , Terlain, B. and Presle, N.
Obesity Affects the Chondrocyte Responsiveness to Leptin in Patients with Osteoarthritis. ,
Arthritis Res Ther
 12 , R112 , 2010
Cells
Chondrocytes
Enzymes
Pronase : 0.15%
Medium
DMEM/F12
Cartilage
Human
Human
Kim Wan-Uk, Kwok Seung-Ki, Hong Kyung-Hee, Yoo Seung-Ah, Kong Jin-Sun, Choe Jongseon, Cho Chul-Soo.
Soluble Fas ligand inhibits angiogenesis in rheumatoid arthritis ,
Arthritis Res Ther
 9 , R42 , 2007
Cells
Synoviocytes
Enzymes
Medium
DMEM
Cartilage
Human
Human, 26-68 year
Marsano A, Millward-Sadler SJ, Salter DM, Adesida A, Hardingham T, Tognana E, Kon E, Chiari-Grisar C, Nehrer S, Jakob M, Martin I.
Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytes ,
Osteoarthritis Cartilage
 15 , 48-58 , 2007
Cells
Meniscus and cartilage
Enzymes
Medium
DMEM
Cartilage
Human
Human, 64-83 yr
Tallheden T, Bengtsson C, Brantsing C, Sjogren-Jansson E, Carlsson L, Peterson L, Brittberg M. and Lindahl A.
Proliferation and Differentiation Potential of Chondrocytes from Osteoarthritic Patients ,
Arthritis Res Ther
 7(3) , R560 , 2005
Cells
Chondrocytes
Enzymes
Medium
DMEM/F12
Cartilage
Human
Human, adult
Liagre B, Vergne-Salle P, Corbiere C, Charissoux JL. and Beneytout JL.
Diosgenin, a Plant Steroid, Induces Apoptosis in Human Rheumatoid Arthritis Synoviocytes with Cyclooxygenase-2 Overexpression ,
Arthritis Res Ther
 6(4) , R373 , 2004
Cells
Synovial
Enzymes
Medium
DMEM
Cartilage
Human
Human, adult
Jakob M, Demarteau O, Schafer D, Stumm M, Heberer M. and Martin I.
Enzymatic Digestion of Adult Human Articular Cartilage Yields a Small Fraction of the Total Available Cells ,
Conn Tissue Res
 44 , 173 , 2003
Cells
Chondrocytes
Enzymes
Medium
DMEM
Cartilage
Human
Human, adults
McEvoy A. , Murphy E. , Ponnio T. , Conneely O. , Bresnihan B. , FitzGerald O. and Murphy E.
Activation of Nuclear Orphan Receptor NURR1 Transcription by NF-kappa B and Cyclic Adenosine 5'-Monophosphate Response Element-binding Protein in Rheumatoid Arthritis Synovial Tissue ,
J Immunol
 168(6) , 2979 , 2002
Cells
Synoviocytes
Enzymes
Medium
RPMI
Cartilage
Human
Human, 15-60 years
Rotter N, Bonassar LJ, Tobias G, Lebl M, Roy AK, Vacanti CA.
Age dependence of cellular properties of human septal cartilage: implications for tissue engineering ,
Arch Otolaryngol Head Neck Surg
 127 , 1248-52 , 2001
Cells
Chondrocytes
Enzymes
Medium
DMEM
Cartilage
Human
Human
Sarkissian M, Lafyatis R.
Integrin engagement regulates proliferation and collagenase expression of rheumatoid synovial fibroblasts ,
J Immunol
 162 , 1772-9 , 1999
Cells
Synovial fibroblasts
Enzymes
Collagenase : 0.4%
Medium
DMEM
Cartilage
Human
Human
Dunham B P, Koch R J.
Basic fibroblast growth factor and insulinlike growth factor I support the growth of human septal chondrocytes in a serum-free environment ,
Arch Otolaryngol Head Neck Surg
 124 , 1325-30 , 1998
Cells
Septal chondrocytes
Enzymes
Medium
DMEM/F12
Cartilage
Human
Human w/ rheumatoid arthritis
Dayer, J. , Krane, S. , Russell, R. and Robinson, D.
Production of Collagenase and Prostaglandins by Isolated Adherent Rheumatoid Synovial Cells ,
Proc Natl Acad Sci U S A
 73 (3) , 945 , 1976
Cells
Synovial tissue
Enzymes
Trypsin : 0.05%
Medium
DMEM
(see reference)
Cartilage
Human
Human, 13-62 years
Srivastava, V.M.L. , Malemud, C.J. , Hough, A.J. , Bland, J.H. and Sokoloff, L.
Preliminary Experience with Cell Culture of Human Articular Chondrocytes ,
Arthritis Rheum
 17 , 165 , 1974
Cells
Articular chondrocytes
Enzymes
Trypsin : 0.2%
Medium
BSS
Cartilage
Human
Human, 26-84 years
Manning, W.K. and Bonner, W.M.
Isolation and Culture of Chondrocytes From Human Adult Articular Cartilage ,
Arthritis Rheum
 10 , 235 , 1967
Cells
Chondrocytes
Medium
GBSS
Liver
Human

Human

Kucykowicz, S. , Amin, O. , Burton, A. , Swadling, L. , Schmidt, N. , Zakeri, N. , Davies, J. , Aidoo-Micah, G. , Stegmann, K. , Easom, N. , Jeffery-Smith, A. , Maini, M. and Pallett, L.
Isolation of Human Intrahepatic Leukocytes for Phenotypic and Functional Characterization by Flow Cytometry. ,
Star Protocols
 3 , 101356 , 2022
Cells
Intrahepatic leukocytes
Enzymes
Medium
RPMI-1640
Liver
Human
Human
Reuschel, E, Jilg, W. , Seelbach-Goebel, B. and Demi, L.
Comparative Purification and Characterization of Hepatitis B Virus-like Particles Produced by Recombinant Vaccinia Viruses in Human Hepatoma Cells and Human Primary Hepatocytes ,
PLoS ONE
 , 2019
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Human
Human
Pfeiffer, E. , Kegel, V. , Zeilinger, K. , Hengstler, J. , Nussler, A. , Seehofer, D. and Damm, G.
Featured Article: Isolation, Characterization, and Cultivation of Human Hepatocytes and Non-Parenchymal Liver Cells. ,
Exp Biol Med (Maywood)
 240 , 645-56 , 2015
Cells
Hepatocytes and non-parenchymal cells
Enzymes
Medium
HBSS
Liver
Human
Human
Werner, M. , Driftmann, S. , Kleinehr, K. , Kaiser, G. , Mathe, Z. , Treckmann, J. , Paul, A. , Skibbe, K. , Timm, J. , Canbay, A. , Gerken, G. , Schlaak, J. and Broering, R.
All-In-One: Advanced Preparation of Human Parenchymal and Non-Parenchymal Liver Cells. ,
PLoS ONE
 10 , e0138655 , 2015
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Human
Human
Lee, S. , Schelcher, C. , Demmel, M. , Hauner, M. and Thasler, W.
Isolation of Human Hepatocytes by a Two-Step Collagenase Perfusion Procedure. ,
J Vis Exp
 , 2013
Cells
Hepatocytes
Enzymes
Collagenase : 0.1-0.15%
Medium
HEPES
Liver
Human
Human, fetal
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Cells
Liver hematopoietic
Enzymes
Medium
RPMI
Liver
Human
Human
Torok, E. , Lutgehetmann, M. , Bierwolf, J. , Melbeck, S. , Dullmann, J. , Nashan, B. , Ma, P. and Pollok, J.
Primary Human Hepatocytes on Biodegradable Poly(l-lactic acid) Matrices: A Promising Model for Improving Transplantation Efficiency with Tissue Engineering. ,
Liver Transpl
 17 , 104 , 2011
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Human
Human
Wauthier, E. , Schmelzer, E. , Turner, W. , Zhang, L. , LeCluyse, E. , Ruiz, J. , Turner, R. , Furth, M. , Kubota, H. , Lozoya, O. , Barbier, C. , McClelland, R. , Yao, H. , Moss, N. , Bruce, A. , Ludlow, J. and Reid, L.
Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance. ,
Methods Cell Biol
 86 , 137 , 2008
Cells
Hepatic stem cells and heptoblasts
Enzymes
Collagenase Type 4 : 0.014-0.06%
Medium
various
Liver
Human
Human
Pichard L, Raulet E, Fabre G, Ferrini JB, Ourlin JC. and Maurel P
Human hepatocyte culture ,
Methods Mol Biol
 320 , 283 , 2006
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HEPES
Liver
Human
Human
Hughes, R. , Mitry, R. , Dhawan, A. , Lehec, S. , Girlanda, R. , Rela, M. , Heaton, N. and Muiesan, P.
Isolation of Hepatocytes from Livers from Non-Heart-Beating Donors for Cell Transplantation ,
Liver Transpl
 12 , 713 , 2006
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
EBSS
Liver
Human
Human
Hussain, S. , Strom, S. , Kirby, M. , Burns, S. , Langemeijer, S. , Ueda, T. , Hsieh, M. and Tisdale, J.
Side Population Cells Derived from Adult Human Liver Generate Hepatocyte-Like Cells In Vitro. ,
Dig Dis Sci
 50 , 1755 , 2005
Cells
Hepatic side population
Enzymes
Collagenase : 0.02-0.05%
Medium
HBSS
Liver
Human
Human, adult
Cho JJ, Joseph B, Sappal BS, Giri RK, Wang R, Ludlow JW, Furth ME, Susick R. and Gupta S.
  Analysis of the functional integrity of cryopreserved human liver cells including xenografting in immunodeficient mice to address suitability for clinical applications ,
Liver Int
 24 , 361 , 2004
Cells
Hepatocytes
Enzymes
Collagenase : 0.6%
Medium
RPMI 1640
Liver
Human
Human, fetal
Malhi, H. , Irani, A. , Gagandeep, S. and Gupta, S.
Isolation of Human Progenitor Liver Epithelial Cells with Extensive Replication Capacity and Differentiation into Mature Hepatocytes. ,
J Cell Sci
 115 , 2679 , 2002
Cells
Epithelial progenitor
Enzymes
Collagenase : 0.03%
Medium
DMEM
Liver
Human
Human
Duanmu Z. , Locke D. , Smigelski J. , Dahn M. , Falany C. , Kocarek T. and Runge-Morris M.
Effects of Dexamethasone on Aryl (SULT1A1)- and hydroxysteroid (SULT2A1)-Sulfotransferase Gene Expression in Primary Cultured Human Hepatocytes ,
Drug Metab Dispos
 30(9) , 997 , 2002
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Human
Human
(also rat)
Dandri, M. , Burda, M. , Torok, E. , Pollok, J. , Iwanska, A. , Sommer, G. and et al.
Repopulation of Mouse Liver with Human Hepatocytes and In Vivo Infection with Hepatitis B Virus ,
Hepatol
 33 , 981 , 2001
Cells
Hepatocytes
Enzymes
Medium
Leffert's buffer
Liver
Human
Human
Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O.
CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers ,
Drug Metab Dispos
 28 , 1321-6 , 2000
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
Williams E
Liver
Human
Human
Parzefall, W. , Erber, E. , Sedivy, R. and Schulte-Hermann, R.
Testing for Induction of DNA Synthesis in Human Hepatocyte Primary Cultures by Rat Liver Tumor Promoters ,
Cancer Res
 51 , 1143 , 1991
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Human
Human
Gomez-Lechon, M. , Lopez, P. , Donato, T. , Montoya, A. , Larrauri, A. , Gimenez, P. , Trullenque, R. , Fabra, R. and Castell, J.
Culture of Human Hepatocytes From Small Surgical Liver Biopsies: Biochemical Characterization And Comparison With in vivo ,
In Vitro Cell Dev Biol
 26 , 67 , 1990
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HEPES buffer
(see reference)
Liver
Human
Human, male, 17-40 yrs.
Begue, J. , Baffet, G. , Campion, J. and Guillouzo, A.
Differential Response of Primary Cultures of Human and Rat Hepatocytes to Aflatoxin B1-Induced Cytotoxicity and Protection by the Hepatoprotective agent(+)-Cyanidanol-3 ,
Biol Cell
 63 , 327 , 1988
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HEPES buffer

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.