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Heart
Rat
Rat, 10 wk
Bishop, S. , Oparil, S. , Reynolds, R. and Drummond, J.
Regional Myocyte Size in Normotensive and Spontaneously Hypertensive Rats ,
Hypertension
 1 (4) , 378 , 1979
Cells
Myocytes
Enzymes
Medium
Saline
Heart
Rat
Rat, male, 200-300 g
Farmer, B. , Harris, R. , Jolly, W. , Hathaway, D. , Katzberg, A. , Watanabe, A. , Whitlow, A. and Besch, H.
Isolation and Characterization of Adult Rat Heart Cells ,
Arch Biochem Biophys
 179 , 545 , 1977
Cells
Myocytes
Enzymes
Hyaluronidase : 82 u/ml
Medium
Kreb's Henseleit buffer
Heart
Rat
Rat, female, albino, 200-250 g
Nag, A. , Fischman, D. , Aumont, M. and Zak, R.
Studies of Isolated Adult Rat Heart Cells: The Surface Morphology and the Influence of Extracellular Calcium Ion Concentration on Cellular Viability ,
Tissue Cell
 9 (3) , 419 , 1977
Cells
Myocytes
Enzymes
Hyaluronidase : 0.1%
Medium
Kreb's Ringers phosphate
Heart
Rat
Rat, adult, male, 150-200g
Grosso, D. , Frangakis, C. , Carlson, E. and Bressler, R.
Isolation and Characterization of Myocytes From the Adult Rat Heart ,
Prep Biochem
 7 (5) , 383 , 1977
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Medium
MEM CF
Heart
Rat
Rat, adult
Moustafa, E. , Skomedal, T. , Osnes, J. and Oye, I.
Cyclic Amp Formation and Morphology of Myocardial Cells Isolated from Adult Heart: Effect of Ca2+ and Mg2+ ,
Biochim Biophys Acta
 421 , 411 , 1976
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Medium
Perfusing solution
Heart
Rat
Rat, SD, albino, male, 180-230 g
Glick, M. , Burns, A. and Reddy, W.
Dispersion and Isolation of Beating Cells from Adult Rat Heart ,
Anal Biochem
 61 , 32 , 1974
Cells
Heart ventricles, beating
Enzymes
Collagenase : 0.05-0.2%
Medium
Phosphate buffer
Heart
Rat
Rat, Hebrew Un strain, 3 & 17 months, 150-210 g
Bierman, E.L. , Stein, O. and Stein, Y.
Lipoprotein Uptake and Metabolism by Rat Aortic Smooth Muscle Cells in Tissue Culture ,
Circ Res
 35 , 136 , 1974
Cells
Myocytes
Enzymes
Trypsin : 0.05%
Medium
Versene buffer
Heart
Rat
Rat, 2-5 day
Speicher, D. and McCarl, R.
Pancreatic Enzyme Requirements for the Dissociation of Rat Hearts for Culture ,
In Vitro
 10 , 30 , 1974
Cells
Myocytes
Enzymes
Trypsin : 0.01%
Medium
Saline A
Heart
Rat
Rat, SD, male, 150-200 g
Pretlow II, T. , Glick, M. and Reddy, W.
Separation of Beating Cardiac Myocytes from Suspensions of Heart Cells ,
Am J Pathol
 67 (2) , 215 , 1972
Cells
Myocytes
Enzymes
Hyaluronidase : 0.05%
Medium
(see reference)
Heart
Rat
Rat, albino, male, 250 g
Berry, M. , Friend, D. and Scheuer, J.
Morphology and Metabolism of Intact Muscle Cells Isolated from Adult Rat Heart ,
Circ Res
 26 , 679 , 1970
Cells
Myocytes
Enzymes
Hyaluronidase : 0.20%
Medium
Hank's solution, CF
Heart
Rat
Rat, adult, male, 250 g
Vahouny, G.V. , Wei, R. , Starkweather, R. and Davis, C.
Preparation of Beating Heart Cells From Adult Rats ,
Science
 167 , 1616 , 1970
Cells
Myocytes
Enzymes
Trypsin : 0.1%
Medium
Saline A
Heart
Rat
Rat, Wistar, 1-4 day old
Mark, G. and Strasser, F.
Pacemaker Activity and Mitosis in Cultures of Newborn Rat Heart Ventricle Cells ,
Exp Cell Res
 44 , 217 , 1966
Cells
Myocytes
Enzymes
Trypsin : 0.125%
Medium
Gey's BSS
Intestine
Rat
Rat, postnatal, adult
Kruger, G. , Mosher, J. , Bixby, S. , Joseph, N. , Iwashita, T. and Morrison, S.
Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness. ,
Neuron
 35 , 657 , 2002
Cells
Gut neural crest stem
Enzymes
Trypsin : 0.025%
Medium
HBSS
Intestine
Rat
Rat, SD, embryonic
Bixby, S. , Kruger, G. , Mosher, J. , Joseph, N. and Morrison, S.
Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity. ,
Neuron
 35 , 643 , 2002
Cells
Sciatic nerve and gut neural crest stem
Enzymes
Trypsin : 0.005%
Medium
HBSS
Intestine
Rat
Rat, male, 18-24 day
Quaroni A, Wands J, Trelstad RL, Isselbacher KJ.
Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria ,
J Cell Biol
 80 , 248-65 , 1979
Cells
Intestinal epithelial
Enzymes
Medium
DMEM
Kidney
Rat
Rat, SD, fetal
Gupta, A. , Jadhav, S. , Tripathy, N. and Nityanand, S.
Fetal Kidney Cells Can Ameliorate Ischemic Acute Renal Failure in Rats through Their Anti-Inflammatory, Anti-Apoptotic and Anti-Oxidative Effects. ,
PLoS ONE
 10 , e0131057 , 2015
Cells
Fetal kidney
Enzymes
Medium
MEM
Kidney
Rat
Rat, Lewis, male
Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J.
Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. ,
Lipids Health Dis
 10 , 171 , 2011
Cells
Renal adipose derived cells
Enzymes
Medium
DMEM
Kidney
Rat
Rat
Panico, C. , Luo, Z. , Damiano, S. , Artigiano, F. , Gill, P. and Welch, W.
Renal Proximal Tubular Reabsorption is Reduced in Adult Spontaneously Hypertensive Rats: Roles of Superoxide and Na+/H+ Exchanger 3. ,
Hypertension
 54 , 1291 , 2009
Cells
Proximal tubule
Enzymes
Medium
see reference
Kidney
Rat
Rat, SD, male, 200-250g
Silva, G. , Beierwaltes, W. and Garvin, J.
Extracellular ATP stimulates NO production in rat thick ascending limb ,
Hypertension
 47 , 563-7 , 2006
Cells
Medullary thick ascending limb
Enzymes
Collagenase : 0.1%
Medium
HEPES-saline
Kidney
Rat
Rat, Wistar, 300g
Deng Aihua, Miracle Cynthia M, Lortie Mark, Satriano Joseph, Gabbai Francis B, Munger Karen A, Thomson Scott C, Blantz Roland C.
Kidney oxygen consumption, carbonic anhydrase, and proton secretion ,
Am J Physiol/Renal
 290 , F1009-15 , 2006
Cells
Renal proximal tubules
Enzymes
Medium
DMEM/F12
Kidney
Rat
Rat, Wistar, male, 300-350 g
Deng, A. , Miracle, C. , Suarez, J. , Lortie, M. , Satriano, J. , Thomson, S. , Munger, K. and Blantz, R.
Oxygen Consumption in the Kidney: Effects of Nitric Oxide Synthase Isoforms and Angiotensin II. ,
Kidney Int
 68 , 723 , 2005
Cells
Renal proximal tubules
Enzymes
Medium
DMEM/F-12
Kidney
Rat
Rat
Arystarkhova, E. , Wetzel R. and Sweadner K.
Distribution and Oligomeric Association of Splice Forms of Na(+)-K(+)-ATPase Regulatory Gamma-subunit in Rat Kidney ,
Am J Physiol/Renal
 282(3) , F393 , 2002
Cells
Cortical tubule cells
Enzymes
Collagenase : 0.6%
Medium
DMEM
Kidney
Rat
Rat, SD, male 275-300 g
Amiri Farhad, Shaw Sean, Wang Xiaodan, Tang Jie, Waller Jennifer L, Eaton Douglas C, Marrero Mario B.
Angiotensin II activation of the JAK/STAT pathway in mesangial cells is altered by high glucose ,
Kidney Int Suppl
 61 , 1605-16 , 2002
Cells
Glomerular mesangial cells
Enzymes
Collagenase Type 1 : 250 u/ml
Medium
DMEM
Kidney
Rat
Rat, SD, 1 week
Valencia L. , Bidet M. , Martial S. , Sanchez E. , Melendez E. , Tauc M. , Poujeol C. , Martin D. , Namorado M. , Reyes J. and Poujeol P.
Nifedipine-activated Ca(2+) Permeability in Newborn Rat Cortical Collecting Duct Cells in Primary Culture ,
Am J Physiol Cell Physiol
 280(5) , C1193 , 2001
Cells
Cortical collection duct
Enzymes
Medium
DMEM/Ham's F12
Kidney
Rat
Rat, SD, 250-300 g
Li, N. , Teggatz, E. , Li, P. , Allaire, R. and Zou, A.
Formation and Actions of Cyclic ADP-Ribose in Renal Microvessels ,
Microvasc Res
 60 , 149 , 2000
Cells
Microvessels
Enzymes
Medium
PSS
Kidney
Rat
Rat, SD, 250-300 g
Mattson D. and Wu F.
Nitric Oxide Synthase Activity and Isoforms in Rat Renal Vasculature ,
Hypertension
 35 , 337 , 2000
Cells
Renal
Enzymes
Medium
see reference
Kidney
Rat
Rat, SD, male, 5-6 week
Miyata, N. , Park, F. , Li, X. and Cowley, A.
Distribution of Angiotensin AT1 and AT2 Receptor Subtypes in the Rat Kidney ,
Am J Physiol
 277 , F437 , 1999
Cells
Renal tubules
Enzymes
Collagenase : 0.1%
Medium
see reference
Kidney
Rat
Rat, SD, 225-250 g
Amiri F, Garcia R.
Regulation of angiotensin II receptors and PKC isoforms by glucose in rat mesangial cells ,
Am J Physiol
 276 , F691-9 , 1999
Cells
Glomerular mesangial cells
Enzymes
Collagenase Type 1 : 250 u/ml
Medium
DMEM
Kidney
Rat
Rat, SD, male
Eitle E, Hiranyachattada S, Wang H, Harris PJ.
Inhibition of proximal tubular fluid absorption by nitric oxide and atrial natriuretic peptide in rat kidney ,
Am J Physiol
 274 , C1075-80 , 1998
Cells
Proximal tubule suspensions
Enzymes
Pronase E : 2 u/ml
Medium
HEPES buffer
Kidney
Rat
Rat, SD, male
Schafer, J. , Watkins, M. , Li, L. , Herter, P. , Haxelmans, S. and Schlatter, E.
A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments ,
Am J Physiol
 273 , F650 , 1997
Cells
Nephron segments
Enzymes
Medium
Eagle's MEM
Kidney
Rat
Rat, 12-weeks-old
Ishikawa, S. , Kusaka, I. , Higashiyama, M. , Nagasaka, S. , Saito, T. , Honda, K. and Saito, T.
Cellular Signaling and Proliferative Action of AVP in Mesangium of SHR: Effect of Low Density Lipoprotein ,
Nat Neurosci
 50 , 1506 , 1996
Cells
Renal
Enzymes
Collagenase : 0.1%
Medium
DMEM
Kidney
Rat
Rat, male, 150-200 g
Elliget, K. and Trump, B.
Primary Cultures of Normal Rat Kidney Proximal Tubule Epithelial Cells for Studies of Renal Cell Injury ,
In Vitro Cell Dev Biol
 27 , 739 , 1991
Cells
Epithelial
Proximal tubule
Enzymes
Protease : 0.1%
Medium
HBSS/CMF
Kidney
Rat
Rat, adult
Brion, L. , Schwartz, J. , Lachman, H. , Zavilowitz, B. and Schwartz, G.
Development of H+ Secretion by Cultured Renal Inner Medullary Collecting Duct Cells ,
Am J Physiol
 257 , F486 , 1989
Cells
Inner medullary collecting duct
Papillae
Enzymes
Medium
PBS
Kidney
Rat
Rat, Wistar, female, 100-150 g
Wang, J. , Kester, M. and Dunn, M.
The Effects of Endotoxin on Platelet-Activating Factor Synthesis in Cultured Rat Glomerular Mesangila Cells ,
Biochim Biophys Acta
 969 , 217 , 1988
Cells
Glomerular mesangial
Enzymes
Collagenase : 0.025%
Medium
RPMI 1640
Kidney
Rat
Rat, Wistar, male, 150 - 200 g
Barlet-Bas, C. , Khadouri, C. , Marsey, S. and Doucet, A.
Sodium-Independent In Vitro Induction of Na+,K+-ATPase by Aldosterone in Renal Target Cells: Permissive Effect of Triiodothyronine ,
Proc Natl Acad Sci U S A
 85 , 1707 , 1988
Cells
Renal target
Enzymes
Collagenase : 1.0% (also 0.7%)
Medium
Eagle's MEM
Kidney
Rat
Rat, Wistar, male, adult
Gesek, F. , Wolff, D. and Strandhov, J.
Improved Separation Method for Rat Proximal and Distal Renal Tubules ,
Am J Physiol
 253 , F358 , 1987
Cells
Proximal tubules
Enzymes
Collagenase : 0.2%
Medium
Krebs-Henseleit buffer

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.