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Tissue References Search Tool

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Heart
Mouse
Mouse, NTg and Tg, 8 - 12 week
Nelson, D. , Setser, E. , Hall, D. , Schwartz, S. , Hewitt, T. , Klevitsky, R. , Osinska, H. , Bellgrau, D. , Duke, R. and Robbins, J.
Proinflammatory Consequences of Transgenic Fas Ligand Expression in the Heart ,
J Clin Invest
 105 (9) , 1199 , 2000
Cells
Myocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
Joklik's MEM
Heart
Mouse
Mouse
Christensen G, Minamisawa S, Gruber PJ, Wang Y, Chien KR.
High-efficiency, long-term cardiac expression of foreign genes in living mouse embryos and neonates ,
Circulation
 101 , 178-84 , 2000
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
Joklik's MEM
Heart
Mouse
Mouse, 2-4 mo
Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP.
Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology ,
Am J Physiol Heart Circ Physiol
 279 , H429-36 , 2000
Cells
Ventricular myocytes
Enzymes
Protease XIV : 0.002%
Medium
MEM
Heart
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Heart
Mouse
Mouse, 1-3 day
Wang, G. and Kang, Y.
Inhibition of Doxorubicin Toxicity in Cultured Neonatal Mouse Cardiomyocytes with Elevated Metallothionein Levels ,
J Pharmacol Exp Ther
 288(3) , 938 , 1999
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.25%
Medium
FBS-MEM
Heart
Mouse
Mouse, 1 day
Lader, A. , Kwiatkowski, D. and Cantiello, H.
Role of Gelsolin in the Actin Filament Regulation of Cardiac L-type Calcium Channels ,
Am J Physiol
 277 , C1277 , 1999
Cells
Cardiomyocytes
Enzymes
NCIS kit : with modifications see reference
Medium
L-15
Heart
Mouse
Mouse, 1 day
Lader, A. , Xiao, Y. , Ishikawa, Y. , Cui, Y. , Vatner, D. , Vatner, S. , Homcy, C. and Cantiello, H.
Cardiac Gsalpha Overexpression Enhances L-type Calcium Channels Through an Adenylyl Cyclase Independent Pathway ,
Proc Natl Acad Sci U S A
 95 , 9669 , 1998
Cells
Cardiomyocytes
Enzymes
NCIS kit : with modifications see reference
Medium
L-15
Heart
Mouse
Mouse (BALB/c), adult
Felzen, B. , Shilkrut, M. , Less, H. , Sarapov, I. , Maor, G. , Coleman, R. , Robinson, R. and et al.
Fas (CD95/Apo-1)-Mediated Damage to Ventricular Myocytes Induced by Cytotoxic T Lymphocytes from Perforin-Deficient Mice ,
Circ Res
 82 , 438 , 1998
Cells
Myocytes
Enzymes
Medium
Tyrode's solution
Heart
Mouse
Mouse, adult
Grupp, I. , Lorenz, J. , Walsh, R. , Boivin, G. and Rindt, H.
Overexpression of Alpha1B-Adrenergic Receptor Induces Left Ventricular Dysfunction in the Absence of Hypertrophy. ,
Am J Physiol
 275 , H1338-50 , 1998
Cells
Cardiomyocytes
Enzymes
Medium
Joklik's MEM
Heart
Mouse
Mouse, P0-P1
Arber, S. , Hunter, J. , Ross, J. , Hongo, M. , Sansig, G. , Borg, J. , Perriard, J. , Chien, K. and Caroni, P.
MLP-Deficient Mice Exhibit a Disruption of Cardiac Cytoarchitectural Organization, Dilated Cadiomyopathy, and Heart Failure ,
Cell
 88 , 393 , 1997
Cells
Cardiomyocytes
Enzymes
Collagenase : 0.045%
Medium
DMEM, Medium 199
Heart
Mouse
Mouse, 6-9 or 21-41 day
Valenzuela, D. , Han, X. , Mende, U. , Fankhauser, C. , Mashimo, H. , Huang, P. , Pfeffer, J. , Neer, E. and Fishman, M.
G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart ,
Proc Natl Acad Sci U S A
 94(5) , 1727 , 1997
Cells
Ventricular and atrial myocytes
Enzymes
Collagenase Type 2 : 95 u/ml
Hyaluronidase : 172.5 u/ml
Trypsin : 0.002%
Medium
DMEM/Tyrodes
Heart
Mouse
Mouse
Soonpaa, M. , Kim, K. , Pajak, aL. , Franklin, M. and Field, L.
Cardiomyocyte DNA Synthesis and Binucleation During Murine Development ,
Am J Physiol
 271 , H2183 , 1996
Cells
Cardiomyocyte
Enzymes
Collagenase : 0.17 %
Medium
PBS
Heart
Mouse
Mouse embryos
Wobus, A. , Kleppisch, T. , Maltsev, V. and Hescheler, J.
Cardiomyocyte-Like Cells Differentiated In Vitro From Embryonic Carcinoma Cells P19 are Characterized by Functional Expression of Adrenoceptors and Ca2+ Channels ,
In Vitro Cell Dev Biol
 30A , 425 , 1994
Cells
Myocytes
Enzymes
Medium
DMEM
Heart
Mouse
Mouse
(also rat, neonate and chick, 8 day embryo)
Gross, W. , Schopf-Ebner, E. and Bucher, O.
Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells ,
Exp Cell Res
 53 , 1 , 1968
Cells
Myocytes
Enzymes
Medium
Rinaldini's buffer solution, CF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.