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Tissue References Search Tool

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Adrenal
Rat
Rat
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Rat
Rat
Gilabert, J, Montalvo, G. and Artalejo A.
Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays ,
Nat Protoc
 , 294 , 2006
Cells
Chromaffin
Enzymes
Medium
HBSS
Adrenal
Rat
Rat, SD
Gilabert, J.
Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture , Cell Biology of the Chromaffin Cell , Borges, R. and Gandia, L. , Instituto Teofilo Hernando , 2004
Cells
Chromaffin cells
Enzymes
Medium
HBSS
Adrenal
Rat
Rat, SD, male
Bruder Eric D, Ball Dennis L, Goodfriend Theodore L, Raff Hershel.
An oxidized metabolite of linoleic acid stimulates corticosterone production by rat adrenal cells ,
Am J Physiol Regul Integr Comp Physiol
 284 , R1631-5 , 2003
Cells
Zona fasciculata/reticularis
Enzymes
Collagenase : 0.4%
Medium
Krebs-HEPES
Adrenal
Rat
Rat, Wistar, newborn
Zhang L, Castell A, Avila E, Drucker-Colín R, Escobar A.
Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons ,
J Neuropathol Exp Neurol
 59 , 170-4 , 2000
Cells
Chromaffin cells
Enzymes
Medium
DMEM
Adrenal
Rat
Rat, SD, male
Sayed, S. , Whitehouse, B. and Jones, P.
Phosphoserine/Threonine Phosphatases in the Rat Adrenal Cortex: A Role in the Control of Steroidogenesis ,
J Endocrinol
 154 , 449 , 1997
Cells
ZG
ZFR
Enzymes
Medium
Kreb's
Adrenal
Rat
Rat, Fischer, male, 10-16 weeks
Roskelley, C.D. and Auersperg, N.
Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture ,
In Vitro Cell Dev Biol
 26 , 493 , 1990
Cells
Adrenocortical
Enzymes
Medium
BSS
Adrenal
Rat
Rat, SD, 2-4 day old
(also bovine)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Adrenal
Rat
Rat, male, 120-160 g,
Rat, SD, male, 400-450 g
Ng, T. and Liu, W.
Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis ,
In Vitro Cell Dev Biol
 26 , 24 , 1990
Cells
Leydig
Adrenal
Enzymes
Collagenase Type 2 : 0.03% (adrenal)
Medium
Krebs Ringer bicarbonate buffer
Adrenal
Rat
Rat, Long-Evans, female, 150-200 g
Payet, N. , Deziel, Y. and Lehoux, J.-G.
Vasopressin: A Potent Growth Factor in Adrenal Glomerulosa Cells in Culture ,
J Steroid Biochem
 20 , 449 , 1984
Cells
Glomerulosa
Enzymes
Collagenase : 0.2%
Medium
MEM-d-Val
Adrenal
Rat
Rat, Fischer, male, 1-10 months
Leonard, R.K. , Auersperg, N. and Parkes, C.O.
Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells ,
In Vitro
 19 , 46 , 1983
Cells
Adrenocortical
Enzymes
Medium
BSS
Adrenal
Rat
Rat, SD, male, 400-450 g
Li, C.H. , Ng, T.B. and Cheng, C.H.K.
Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells ,
Int J Pept Protein Res
 19 , 361 , 1982
Cells
Decapular
Capsular
Glomerulosa
Enzymes
Medium
Medium 199
Adrenal
Rat
Rat, Hanover-Wistar, 2nd postnatal week (also guinea-pig, cattle)
Unsicker, K. , Rieffert, B. and Ziegler, W.
Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro ,
Adv Biochem Psychopharmacol
 255 , 51 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.5%
Collagenase : 0.5%
Medium
HBSS
Adrenal
Rat
Rat , SD, female, 200 g
Braley, L. , Williams, G. and Bradwin, G.
The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells ,
Endocrinology
 106 (1) , 50 , 1980
Cells
Glomerulosa
Enzymes
Medium
Kreb's
Adrenal
Rat
Rat
Englert, D.F.
An Optical Study of Isolated Rat Adrenal Chromaffin Cells ,
Exp Cell Res
 125 , 369 , 1980
Cells
Chromaffin
Enzymes
Trypsin : 0.10%
Medium
Ham's F-12 w/HEPES
Adrenal
Rat
Rat
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Adrenal
Rat
Rat, Wistar-Hanover, 7-12 day
Unsicker, K. , Krisch, B. , Otten, U. and Thoenen, H.
Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids ,
Proc Natl Acad Sci U S A
 75 (7) , 3498 , 1978
Cells
Medullary
Enzymes
Trypsin : 0.125%
Medium
HBSS
Adrenal
Rat
Rat, SD, male
Barofsky, A. , Feinstein, M. and Halkerston, I.
Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands ,
Exp Cell Res
 79 , 263 , 1973
Cells
Cortical
Enzymes
Trypsin : 0.25%
Medium
Kreb's Ringer bicarbonate buffer
Adrenal
Rat
Rat, Holtzman, male, 180-250 g
Kloppenborg, P. , Island, D. , Liddle, G. , Michelakis, A. and Nicholson, W.
A Method of Preparing Adrenal Cell Suspensions and Its Applicability to the In Vitro Study of Adrenal Metabolism ,
Endocrinology
 82 , 1053 , 1968
Cells
Adrenal
Enzymes
Medium
Kreb's Ringer bicarbonate buffer
Liver
Rat

Rat, 8-14 week

Ge, J. , Zheng, Y. , Tsuchida, T. , Furuya, K. , Isoda, H. , Taniguchi, H. , Ohkohchi, N. and Oda, T.
Hepatic Stellate Cells Contribute to Liver Regeneration through Galectins in Hepatic Stem Cell Nliche. ,
Stem Cell Res Ther
 11 , 425 , 2020
Cells
Parenchymal and non-parenchymal
Enzymes
Collagenase : 0.05%
Medium
Williams E
Liver
Rat

Wistar, male, 200-250 g

Berardo, C. , Ferrigno, A. , Siciliano, V. , Richelmi, P. , Vairetti, M. and Pasqua, L.
Isolation of Rat Hepatocytes for Pharmacological Studies on Metabotropic Glutamate Receptor (mGluR) Subtype 5: A Comparison Between Collagenase I Versus Collagenase IV. ,
Eur J Histochem
 64 , 2020
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Rat
Rat, SD, 500-700g
Perepelyuk, M. , Terajima, M. , Wang, A. , Georges, P. , Janmey, P. , Yamauchi, M. and Wells, R.
Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury. ,
Am J Physiol Gastrointest Liver Physiol
 304 , G605 , 2013
Cells
Hepatocytes and sinusoidal endothelial
Enzymes
Medium
HBSS
Liver
Rat
Rat, adult
Shen, L. , Hillebrand, A. , Wang, D. and Liu, M.
Isolation and Primary Culture of Rat Hepatic Cells. ,
J Vis Exp
 64 , e3917 , 2012
Cells
Hepatocytes
Enzymes
Medium
William's
Liver
Rat
Rat, adult
Wen, J. , Olsen, A. , Perepelyuk, M. and Wells, R.
Isolation of Rat Portal Fibroblasts by In Situ Liver Perfusion. ,
J Vis Exp
 64 , e3669 , 2012
Cells
Portal fibroblasts
Enzymes
Medium
DMEM/F-12
Liver
Rat
Rat
Budick-Harmelin, N. , Anavi, S. , Madar, Z. and Tirosh, O.
Fatty Acids-Stress Attenuates Gluconeogenesis Induction and Glucose Production in Primary Hepatocytes. ,
Lipids Health Dis
 11 , 66 , 2012
Cells
Hepatocytes
Enzymes
Medium
DMEM
Liver
Rat
Rat, SD, 240-320g
Pillai, V. and Mehvar, R.
Inhibition of NADPH-Cytochrome P450 Reductase by Tannic Acid in Rat Liver Microsomes and Primary Hepatocytes: Methodological Artifacts and Application to Ischemia-Reperfusion Injury ,
J Pharm Sci
 100 , 3495 , 2011
Cells
Hepatocytes
Enzymes
HIS kit : per instructions
Medium
Waymouth's MB
Liver
Rat
Rat
Gopalakrishnan, S. and Harris, E.
In Vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion. ,
J Vis Exp
 57 , e3138 , 2011
Cells
Hepatocytes and SEC's
Enzymes
Collagenase Type 1 : 0.1-0.2%
Medium
RPMI
Liver
Rat
Rat, SD, 250-300g
Parasrampuria, R. and Mehvar, R.
Dose-Dependent Inhibition of Transporter-Mediated Hepatic Uptake and Biliary Excretion of Methotrexate by Cyclosporine A in an Isolated Perfused Rat Liver Model. ,
J Pharm Sci
 99 , 5060 , 2010
Cells
Hepatocytes
Enzymes
HIS kit : per instructions
Medium
Krebs-Henseleit
Liver
Rat
Rat, SD
Handy, J. , Saxena, N. , Fu, P. , Lin, S. , Mells, J. , Gupta, N. and Anania, F.
Adiponectin Activation of AMPK Disrupts Leptin-Mediated Hepatic Fibrosis via Suppressors of Cytokine Signaling (SOCS-3). ,
J Cell Biochem
 110 , 1195 , 2010
Cells
Hepatic stellate cells
Enzymes
Pronase : 0.02%
Collagenase : see reference
Medium
Medium 199
Liver
Rat
Rat, Wistar, female
Zvibel, I. , Atias, D. , Phillips, A. , Halpern, Z. and Oren, R.
Thyroid Hormones Induce Activation of Rat Hepatic Stellate Cells Through Increased Expression of p75 Neurotrophin Receptor and Direct Activation of Rho. ,
Lab Invest
 90 , 674 , 2010
Cells
Hepatic stellate cells
Enzymes
Collagenase Type 1 : 0.025-0.1%
Pronase : 0.025-0.13%
Medium
DMEM
Liver
Rat
Rat, SD
Chung, C. , Shugrue, C. , Nagar, A. , Doll, J. , Cornwell, M. , Gattu, A. , Kolodecik, T. , Pandol, S. and Gorelick, F.
Ethanol Exposure Depletes Hepatic Pigment Epithelium-Derived Factor, a Novel Lipid Regulator. ,
Gastroenterol
 136 , 331 , 2009
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HBSS
Liver
Rat
Rat, Lewis, male
Bettinger, C. , Kulig, K. , Vacanti, J. , Langer, R. and Borenstein, J.
Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture. ,
Tissue Eng Part A
 15 , 1321 , 2009
Cells
Hepatocytes
Enzymes
Medium
DPBS
Liver
Rat
Rat, 200-300 g
Mula, N. , Cubero, F. , Codesal, J. , de Andres, S. , Escudero, C. , Garcia-Barrutia, S. , Millan, I. , Arahuetes, R. and Maganto, P.
Survival of Allogeneic Hepatocytes Transplanted into the Thymus. ,
Cells Tissues Organs
 188 , 270 , 2008
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Rat
Rat, SD, 500-750 g
Li, Z. , Dranoff, J. , Chan, E. , Uemura, M. and Sevigny, J.and Wells, R.
Transforming Growth Factor-beta and Substrate Stiffness Regulate Portal Fibroblast Activation in Culture. ,
Hepatol
 46 , 1246-56 , 2007
Cells
Portal fibroblasts
Enzymes
Collagenase : 0.03%
Pronase : 0.033%
Hyaluronidase : 0.036%
Medium
DMEM/F-12
Liver
Rat
Rat, SD, 180-200g
Charbonneau, A. , Unson, C. and Lavoie, J.
High-Fat Diet-Induced Hepatic Steatosis Reduces Glucagon Receptor Content in Rat Hepatocytes: Potential Interaction with Acute Exercise. ,
J Physiol
 579 , 255 , 2007
Cells
Hepatocytes
Enzymes
Medium
see reference
Liver
Rat
Rat, SD, male, 230-250g
Doleh, L. and Romani, A.
Biphasic Effect of Extra-Reticular Mg2+ on Hepatic G6P Transport and Hydrolysis. ,
Arch Biochem Biophys
 467 , 283 , 2007
Cells
Hepatocytes
Enzymes
Medium
HEPES

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.