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Tissue References Search Tool

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Endothelial
Rat
Rat, SD, male, 250-300 g
Schwertschlag, U.S. and Whorton, A.R.
Platelet-Activating Factor-Induced Homologous and Heterologous Desensitization In Cultured Vascular Smooth Muscle Cells ,
J Biol Chem
 263 , 13791 , 1988
Cells
Smooth muscle, aorta
Enzymes
Medium
HBSS with 0.2 mM Ca++
Endothelial
Rat
Rat, SD, male, 350 - 450 g
Friedman, S. and Roll, F.
Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan ,
Anal Biochem
 161 , 207 , 1987
Cells
Lipocytes
Kupffer
Sinusoidal endothelial
Enzymes
Collagenase : 0.015%
Medium
DMEM/Ham's F-12
Endothelial
Rat
Rat, Wistar, male, 3 mo
Nagelkenke, J. , Barto, K. and VanBerkel, T.
In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells ,
J Biol Chem
 258 , 12221 , 1983
Cells
Endothelial
Kupffer
Parenchymal
Enzymes
Collagenase : 0.05%
Pronase : 0.25%
Medium
HBSS
Endothelial
Rat
Rat, Wistar-Kyoto, male, 100 - 200 g
Diglio, C.A. , Grammas, P. , Filiberto Giacomelli, M.S. and Wiener, J.
Primary Culture of Rat Cerebral Microvascular Endothelial Cells ,
Lab Invest
 46 , 554 , 1982
Cells
Endothelial
Cerebral
Enzymes
Medium
HBSS
Endothelial
Rat
Rat, 300 G, and pig, 30-40 Kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA
Endothelial
Rat
Rat
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Endothelial
Rat
Rat, Wistar, adult, 170 g
Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.
Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
 129 , 261 , 1979
Cells
Endothelial
Enzymes
Trypsin : 0.5%
Medium
BSS
Parotid
Rat
Rat, Wistar, male, 130 g
Looms, D. , Dissing, S. , Tritsaris, K. , Pedersen, A. and Nauntofte, B.
Adrenoceptor-Activated Nitric Oxide Synthesis in Salivary Acinar Cells. ,
Adv Dent Res
 14 , 62 , 2000
Cells
Acinar
Enzymes
Collagenase : 75 u/ml
Hyaluronidase : 153 u/ml
Medium
RPMI 1640
Parotid
Rat
Rat, SD, 100-120 g
D'Silva NJ, DiJulio DH, Belton CM, Jacobson KL, Watson EL.
Immunolocalization of Rap1 in the rat parotid gland: detection on secretory granule membranes ,
J Histochem Cytochem
 45 , 965-73 , 1997
Cells
Parotid acinar cells
Enzymes
Collagenase Type 2 : 90 u/ml
Medium
Krebs-Henseleit Bicarbonate
Parotid
Rat
Rat, SD, male
Prasad, K.N. , Edwards-Prasad, J. , Carvaiho, I. , LaRosa, F.G. , Baibinder, E. , Meyers, A. and Quissell, D.
Establishment of Primary Cultures of Rat and Human Parotid Epithelial Cells for Transfection Experiments ,
In Vitro Cell Dev Biol
 28 , 493 , 1992
Cells
Epithelial
Enzymes
Collagenase : 50-75 u/ml
Hyaluronidase : 0.1%
Medium
HBSS CF
Parotid
Rat
Rat, SD, male, 100-150 g
Yeh, C. , Mertz, P. , Oliver, C. , Baum, B. and Kousvelari, E.
Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells ,
In Vitro Cell Dev Biol
 27 , 707 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.001%
Medium
F12 medium
Parotid
Rat
Rat, Wistar, male
Foskett, J. , Roifman, C. and Wong, D.
Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells ,
J Biol Chem
 266 , 2778 , 1991
Cells
Parotid acinar
Enzymes
Trypsin : 0.02%
Medium
Solution B
(See reference)
Parotid
Rat
Rat
Takuma, T. and Ichida, T.
Amylase Secretion From Saponin-Permeabilized Parotid Cells Evoked by Cyclic AMP ,
J Invest Dermatol
 103 , 95 , 1988
Cells
Parotid
Enzymes
Hyaluronidase : 0.025%
Medium
HBSS with 20mM HEPES
Parotid
Rat
Rat, Wistar, male, 250 - 350 g
Melvin, J. , Kawaguchi, M. , Baum, B. and Turner, R.
A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells ,
Biochem Biophys Res Commun
 145 , 754 , 1987
Cells
Acinar, parotid
Enzymes
Hyaluronidase : 0.015%
Medium
Earle's MEM
Parotid
Rat
Rat, SD, male, 50 - 125 g
Oliver, C. , Waters, J. , Tolbert, C. and Kleinman, H.
Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel ,
In Vitro Cell Dev Biol
 23 , 465 , 1987
Cells
Acinar
Exorbital lacrimal, parotid, pancreas
Enzymes
Trypsin : 0.01%
Medium
HBSS, CMF
Parotid
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.