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Heart
Rat
Rat, Wistar, 2-4 day neonates
Grynberg, A. , Degois, M. , Guenot, L. and Athias, P.
Primary Rat Cardiac Cell Culture: Diet of the Mother Rats as a Determinant Parameter of Cardiomyoblast Production from Neonates ,
Biol Cell
 57 , 89 , 1986
Cells
Myoblast, cardiac
Enzymes
Trypsin : 0.1%
Medium
Standardized Medium
(see reference)
Heart
Rat
Rat, SD, timid-pregnant
Freerksen, D. , Schroedl, N. and Hartzell, C.
Control of Enzyme Activity Levels by Serum and Hydrocortisone in Neonatal Rat Heart Cells Cultured in Serum-Free Medium ,
J Cell Physiol
 120 , 126 , 1984
Cells
Myocytes
Enzymes
Trypsin : 2.4 u/ml
Medium
DMEM
Heart
Rat
Rat, SD, female, 12 weeks
Piper, H.M. , Probst, I. , Schwartz, P. , Hutter, F.J. and Spieckermann, P.G.
Culturing of Calcium Stable Adult Cardiac Myocytes ,
J Mol Cell Cardiol
 14 , 397 , 1982
Cells
Myocytes
Enzymes
Medium
Krebs Ringer bicarbonate buffer
Heart
Rat
Rat, male/female, 200-250 g
Nag, A. and Cheng, M.
Adult Mammalian Cardiac Muscle Cells in Culture ,
Tissue Cell
 13 , 515 , 1981
Cells
Muscle
Enzymes
Hyaluronidase : 0.1%
Medium
Krebs Ringer phosphate buffer, CMF
Heart
Rat
Rat, SD, adult, male, 200-250 g
Frangakis, C. , Bahl, J. , McDaniel, H. and Bressler, R.
Tolerance to Physiological Calcium by Isolated Mycocytes from the Adult Rat Heart; An Improved Cellular Preparation ,
Life Sci
 27 , 815 , 1980
Cells
Myocytes
Enzymes
Hyaluronidase : 0.10%
Medium
Joklik MEM, CF
Heart
Rat
Rat, SD, female, 12 wks, 250 g
Powell, T. , Terrar, D.A. and Twist, V.W.
Electrical Properties of Individual Cells Isolated From Adult Rat Ventricular Myocardium ,
J Physiol
 302 , 131 , 1980
Cells
Myocytes
Ventricular myocardium
Enzymes
Medium
Kreb's Ringer bicarbonate buffer
Heart
Rat
Rat, female, adult, 200 - 300 g
Claycomb, W.C. and Palazzo, M.C.
Culture of The Terminally Differentiated Adult Cardiac Muscle Cell: A Light and Scanning Electron Microscope Study ,
Dev Biol
 80 , 466 , 1980
Cells
Myocytes
Enzymes
Medium
Joklik's MEM
Heart
Rat
Rat, 10 wk
Bishop, S. , Oparil, S. , Reynolds, R. and Drummond, J.
Regional Myocyte Size in Normotensive and Spontaneously Hypertensive Rats ,
Hypertension
 1 (4) , 378 , 1979
Cells
Myocytes
Enzymes
Medium
Saline
Heart
Rat
Rat, male, 200-300 g
Farmer, B. , Harris, R. , Jolly, W. , Hathaway, D. , Katzberg, A. , Watanabe, A. , Whitlow, A. and Besch, H.
Isolation and Characterization of Adult Rat Heart Cells ,
Arch Biochem Biophys
 179 , 545 , 1977
Cells
Myocytes
Enzymes
Hyaluronidase : 82 u/ml
Medium
Kreb's Henseleit buffer
Heart
Rat
Rat, female, albino, 200-250 g
Nag, A. , Fischman, D. , Aumont, M. and Zak, R.
Studies of Isolated Adult Rat Heart Cells: The Surface Morphology and the Influence of Extracellular Calcium Ion Concentration on Cellular Viability ,
Tissue Cell
 9 (3) , 419 , 1977
Cells
Myocytes
Enzymes
Hyaluronidase : 0.1%
Medium
Kreb's Ringers phosphate
Heart
Rat
Rat, adult, male, 150-200g
Grosso, D. , Frangakis, C. , Carlson, E. and Bressler, R.
Isolation and Characterization of Myocytes From the Adult Rat Heart ,
Prep Biochem
 7 (5) , 383 , 1977
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Medium
MEM CF
Heart
Rat
Rat, adult
Moustafa, E. , Skomedal, T. , Osnes, J. and Oye, I.
Cyclic Amp Formation and Morphology of Myocardial Cells Isolated from Adult Heart: Effect of Ca2+ and Mg2+ ,
Biochim Biophys Acta
 421 , 411 , 1976
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Medium
Perfusing solution
Heart
Rat
Rat, SD, albino, male, 180-230 g
Glick, M. , Burns, A. and Reddy, W.
Dispersion and Isolation of Beating Cells from Adult Rat Heart ,
Anal Biochem
 61 , 32 , 1974
Cells
Heart ventricles, beating
Enzymes
Collagenase : 0.05-0.2%
Medium
Phosphate buffer
Heart
Rat
Rat, Hebrew Un strain, 3 & 17 months, 150-210 g
Bierman, E.L. , Stein, O. and Stein, Y.
Lipoprotein Uptake and Metabolism by Rat Aortic Smooth Muscle Cells in Tissue Culture ,
Circ Res
 35 , 136 , 1974
Cells
Myocytes
Enzymes
Trypsin : 0.05%
Medium
Versene buffer
Heart
Rat
Rat, 2-5 day
Speicher, D. and McCarl, R.
Pancreatic Enzyme Requirements for the Dissociation of Rat Hearts for Culture ,
In Vitro
 10 , 30 , 1974
Cells
Myocytes
Enzymes
Trypsin : 0.01%
Medium
Saline A
Heart
Rat
Rat, SD, male, 150-200 g
Pretlow II, T. , Glick, M. and Reddy, W.
Separation of Beating Cardiac Myocytes from Suspensions of Heart Cells ,
Am J Pathol
 67 (2) , 215 , 1972
Cells
Myocytes
Enzymes
Hyaluronidase : 0.05%
Medium
(see reference)
Heart
Rat
Rat, albino, male, 250 g
Berry, M. , Friend, D. and Scheuer, J.
Morphology and Metabolism of Intact Muscle Cells Isolated from Adult Rat Heart ,
Circ Res
 26 , 679 , 1970
Cells
Myocytes
Enzymes
Hyaluronidase : 0.20%
Medium
Hank's solution, CF
Heart
Rat
Rat, adult, male, 250 g
Vahouny, G.V. , Wei, R. , Starkweather, R. and Davis, C.
Preparation of Beating Heart Cells From Adult Rats ,
Science
 167 , 1616 , 1970
Cells
Myocytes
Enzymes
Trypsin : 0.1%
Medium
Saline A
Heart
Rat
Rat, Wistar, 1-4 day old
Mark, G. and Strasser, F.
Pacemaker Activity and Mitosis in Cultures of Newborn Rat Heart Ventricle Cells ,
Exp Cell Res
 44 , 217 , 1966
Cells
Myocytes
Enzymes
Trypsin : 0.125%
Medium
Gey's BSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.