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Tissue References Search Tool

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Pancreas
Rat
Rat, Wistar, male, 200 - 300 g
Shibata, A. , Ludvigsen, C. , Naber, S. , McKaneil M. and Lacy, P.
Standardization of a Digestion-Filtration Method for Isolation of Islets ,
Diabetes
 25 , 667 , 1976
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF
Pancreas
Rat
Rat, Wistar-Lewis, fetal 18-20 day gestation
(also neonatal 3-12 day old)
Braaten, J. , Jarlfors, U. , Smith, D. and Mintz, D.
Purification of Monolayer Cell Cultures of the Endocrine Pancreas ,
Tissue Cell
 7 (4) , 747 , 1975
Cells
Islets
Enzymes
Medium
EBSS
(see reference)
Pancreas
Rat
Rat, neonate
Leonard, R. , Lazarow, A. and Hegre, O.
Pancreatic Islet Transplantation in the Rat ,
Diabetes
 22 , 413 , 1973
Cells
Islets
Enzymes
Trypsin : 0.05%
Medium
Puck's saline buffered w/ EDTA 0.02%
Pancreas
Rat
Rat, male, albino, 200-300 g
Lacy, P. , Walker, M. and Fink, J.
Perifusion of Isolated Rat Islets in Vitro ,
Diabetes
 21 (10) , 987 , 1972
Cells
Islets
Enzymes
Collagenase : 0.5%
Medium
Hanks solution
Pancreas
Rat
Rat, Lewis, inbred
Ballinger, W. and Lacy, P.
Transplantation of Intact Pancreatic Islets in Rats ,
Surgery
 72 (2) , 175 , 1972
Cells
Islets
Enzymes
Collagenase : 0.5%
Medium
HBSS
Pancreas
Rat
Rat, Wistar, albino, male, 400 - 500 g
Lacy, P. and Kostianovsky, M.
Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas ,
Diabetes
 16 , 35 , 1967
Cells
Islets
Enzymes
Collagenase Type 4 : 1.0% - 1.2%
Medium
HBSS
Pancreas
Rat
Rat
Essner, E.
Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands ,
Exp Cell Res
 7 , 430 , 1954
Cells
Ascites hepatoma
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer (see reference)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.