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Tissue References Search Tool

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Pituitary
Bovine
Calf, male, less then 6 months old
Hassan, H. and Merkel, R.
Perifusion Model System to Culture Bovine Hypothalamic Slices In Series with Dispersed Anterior Pituitary Cells ,
In Vitro Cell Dev Biol
 30A , 435 , 1994
Cells
Pituitary
Enzymes
Collagenase : 0.3%
Medium
DMEM
Pituitary
Bovine
Bovine
Mason, W. and Ingram, C.
Techniques for Studying the Role of Electrical Activity in Control of Secretion by Normal Anterior Pituitary Cells , Vol. 124 , 207 , 1986
Cells
Pituitary
Enzymes
Collagenase : 0.1%
Medium
EBSS, CMF
Pituitary
Bovine
Bovine
Ferrara, N. , Goldsmith, P. , Fujii, D. and Weiner, R.
Culture and Characterization of Follicular Cells of the Bovine Anterior Pituitary and Pars Tuberalis , Vol. 124 , 245 , 1986
Cells
Follicular, anterior pituitary and pars tuberalis
Enzymes
Deoxyribonuclease I : 200 µg/ml
Medium
HBSS, CMF
Pituitary
Bovine
Calf, male, 1-6 week old
Ridgway, E. , Klibanski, A. , Marorana, M. , Milbury, P. , Kieffer, J. and Chin, W.
The Effect of Somatostatin on the Release of Thyrotropin and its Subunits from Bovine Anterior Pitituary Cells in Vitro ,
Endocrinology
 112 (6) , 1937 , 1983
Cells
Pitituary
Enzymes
Hyaluronidase : 0.1%
Medium
DMEM
Pituitary
Mouse
Mouse
Perez Millain, M. , Brinkmeier, M. , Mortensen, A. and Camper, S.
PROP1 Triggers Epithelial-Mesenchymal Transition-Like Process in Pituitary Stem Cells. ,
Elife
 5 , 2016
Cells
Pituitary
Enzymes
Medium
HBSS
Pituitary
Mouse
Mouse
Pyczek, J. , Buslei, R. , Schult, D. , Holsken, A. , Buchfelder, M. , Hess, I. , Hahn, H. and Uhmann, A.
Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland. ,
Sci Rep
 6 , 24928 , 2016
Cells
Pituitary
Enzymes
Medium
DMEM
Pituitary
Mouse
Mouse, male
Steveson Tami C, Ciccotosto Giuseppe D, Ma Xin-Ming, Mueller Gregory P, Mains Richard E, Eipper Betty A.
Menkes protein contributes to the function of peptidylglycine alpha-amidating monooxygenase ,
Endocrinology
 144 , 188-200 , 2003
Cells
Pituitary
Enzymes
Collagenase : 0.4%
Hyaluronidase : 0.1%
Trypsin : 0.3%
Medium
DMEM/Han's F12
Pituitary
Ovine
Ovine, adult
Xu Ruwei, Wang Qinling, Yan Ming, Hernandez Maria, Gong Changhong, Boon WahChin, Murata Yoko, Ueta Yoichi, Chen Chen.
Orexin-A augments voltage-gated Ca2+ currents and synergistically increases growth hormone (GH) secretion with GH-releasing hormone in primary cultured ovine somatotropes ,
Endocrinology
 143 , 4609-19 , 2002
Cells
Somatotropes
Enzymes
Medium
Medium 199
Pituitary
Rat
Rat, male, 12-15 week
Akieda-Asai, S. , Zaima, N. , Ikegami, K. , Kahyo, T, Yao, I. , Hatanaka, T. , Iemura, S. , Sugiyama, R. , Yokozeki, T. , Eishi, Y. , Koike, M. , Ikeda, K. , Chiba, T. , Yamaza, H. , Shimokawa, I. , Song, S. , Matsuno, A. , Mizutani, A. , Sawabe, M. Chao, N. and Tanaka, M.
SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5K gamma Activity through Deacetylation of Specific Lysine Residues in Mammals. ,
PLoS ONE
 5 , e11755 , 2010
Cells
Pituitary
Enzymes
Medium
DMEM
Pituitary
Rat
Rat, Wistar, male, 2 month old
Zhou, X. , De Schepper, J. , De Craemer, D. , Delhase, M. , Gys, G. , Smitz, J. and Hooghe-Peters, E.
Pituitary Growth Hormone Release and Gene Expression in Cafeteria-Diet-Induced Obese Rats ,
J Endocrinol
 159 , 165 , 1998
Cells
Pituitary
Enzymes
Trypsin : 0.1%
Medium
DMEM
Pituitary
Rat
Rat, SD, female, 200 - 250 g
D'Emden, M. and Wark, J.
Culture Requirements for Optimal Expression of 1,25-Dihydroxyvitamin D3-Enhanced Thyrotropin Secretion ,
In Vitro Cell Dev Biol
 27 , 197 , 1991
Cells
Anterior pituitary gland
Enzymes
Trypsin : 0.1%
Medium
EBSS, CMF
Pituitary
Rat
Rat
Wilfinger, W. , Larsen, W. , Downs, T. and Wilbur, D.
An In Vitro Model for Studies of Intercellular Communication in Cultured Rat Anterior Pituitary Cells ,
Tissue Cell
 16 (4) , 483 , 1984
Cells
Pituitary
Enzymes
Trypsin : 0.3 %
Medium
HEPES
Pituitary
Rat
Rat, SD, male, 250-450 g
Portanova, R. , Smith, D. and Sayers, G.
A Trypsin for the Preparation of Isolated Rat Anterior Pituitary ,
Proc Soc Exp Biol Med
 133 , 573 , 1970
Cells
Anterior pituitary
Enzymes
Trypsin : 0.25%
Medium
Krebs
Reproductive
Bovine
Bovine
Levy N, Gordin M, Mamluk R, Yanagisawa M, Smith M F, Hampton J H, Meidan R.
Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum: implications for luteolysis ,
Endocrinology
 142 , 5254-60 , 2001
Cells
Corpus leuteal cells
Enzymes
Collagenase Type 4 : 420 u/ml
Medium
M-199
Reproductive
Bovine
Bovine, female
Tsang PC, Poff JP, Boulton EP, Condon WA.
Four-day-old bovine corpus luteum: progesterone production and identification of matrix metalloproteinase activity in vitro ,
Biol Reprod
 53 , 1160-8 , 1995
Cells
Leuteal
Enzymes
Medium
Ham's F-12
Reproductive
Bovine
Calf, mid to late gestational
Coplen, D. , Howard, P. , Duckett, J. , Snyder, H. and Macarak, E.
Characterization of a Fibroblast Cell From the Urinary Bladder Wall ,
In Vitro Cell Dev Biol
 30A , 604 , 1994
Cells
Fibroblasts
Enzymes
Collagenase : 0.1%
Medium
Medium 199
Reproductive
Bovine
Bovine
Munson, L. , Chandler, S. and Schlafer, D.
Long-Term Culture of Bovine Trophoblastic Cells ,
J Tiss Cul Meth
 11 (3) , 123 , 1988
Cells
Epithelial
Endometrial
Enzymes
Medium
DMEM/EBSS
Reproductive
Bovine
Bovine
(also porcine, human)
Marcus, G. , Connor, L. , Domingo, M. , Tsang, B. , Downey, B. and Ainsworth, L.
Enzymatic Dissociation of Ovarian and Uterine Tissues ,
Endocr Res
 10 , 151 , 1984
Cells
Interna & corpus luteum
Endometrium
Ovarian
Uterine
Enzymes
Pronase : 0.1%
Medium
Moscona's BSS
Reproductive
Canine
Canine
Park, S. , Seo, M. , Kim, H. and Kang, K.
Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells. ,
PLoS ONE
 7 , e44693 , 2012
Cells
Amniotic membrane MSC
Enzymes
Trypsin : 0.25%
Medium
LG-DMEM
Reproductive
Chicken

Chicken, male, 1 day

Huang, H. , Wang, M. , Hou, L. , Lin, X. , Pan, S. , Zheng, P. and Zhao, Q.
A Potential Mechanism Associated with Lead-induced Spermatogonia and Leydig Cell Toxicity and Mitigative Effect of Selenium in Chicken. , Ecotoxicol Environ Saf Vol. 209 , 111671 , 2021
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Trypsin : 0.1%
Medium
DMEM
Reproductive
Chicken
Chicken, Gallus Domesticus,20-30 week
Du Meihong, Han Haitang, Jiang Bin, Zhao Chen, Qian Changsong, Shen Haiyan, Xu Yan, Li Zandong.
An efficient isolation method for domestic hen (Gallus domesticus) ovarian primary follicles ,
J Reprod Dev
 52 , 569-76 , 2006
Cells
Primary follicles
Enzymes
Trypsin : 0.15%
Medium
Dulbecco's phosphate buffered saline
Reproductive
Fish
Sebastes Schlegel
Ryu, J. , Kim, H. , Bae, S. , Jung, C. and Gong, S.
Isolation and in Vitro Culture of Primary Cell Populations Derived from Ovarian Tissues of the Rockfish, Sebastes schlegeli ,
Fish Aquatic Sci
 19 , 2016
Cells
Ovarian
Enzymes
Collagenase Type 1 : 500 u/ml
Medium
L-15
Reproductive
Frog

Frog, X. laevis

Lavering, E. , Petros, I. and Weeks, D.
Component Analysis of Nucleolar Protein Compartments using Xenopus laevis Oocytes. , Dev Growth Differ Vol. 64 , 306-317 , 2022
Cells
Xenopus oocytes
Enzymes
Medium
Ovary Ringers Solution
Reproductive
Frog
Xenopus Laevis, female
Nicoll, G, Jawad, A. , Weymouth, R, Zhang, H. and Beg, A.
Pharmacological characterization of the excitatory ‘Cys-loop’ GABA receptor family in Caenorhabditis elegans ,
Br J Pharmacol
 174 , 781 , 2017
Cells
Oocytes
Enzymes
Medium
Barth's solution
Reproductive
Frog
Xenopus laevis
Chatzidaki, A. , D'Oyley, J. , Gill-Thind, J. , Sheppard, T. and Millar, N.
The Influence of Allosteric Modulators and Transmembrane Mutations on Desensitisation and Activation of alpha7 Nicotinic Acetylcholine Receptors. ,
Neuropharmacol
 97 , 75-85 , 2015
Cells
Oocytes
Enzymes
Collagenase : 0.2%
Medium
Barth's solution
Reproductive
Frog
Xenopus
O'Connell, D. , Mruk, K. , Rocheleau, J. and Kobertz, W.
Xenopus laevis Oocytes Infected with Multi-drug-resistant Bacteria: Implications for Electrical Recordings. ,
J Gen Physiol
 138 , 271-7 , 2011
Cells
Oocytes
Enzymes
Medium
see reference
Reproductive
Frog
Xenopus laevis, female
Cohen, S. , Au, S. and Pante, N.
Microinjection of Xenopus Laevis Oocytes. ,
J Vis Exp
 24 , 1106 , 2009
Cells
Oocytes
Enzymes
Collagenase : 0.5%
Medium
Barth's solution, CF
Reproductive
Frog
Xenopus laevis, female
Mruk, K. and Kobertz, W.
Discovery of a Novel Activator of KCNQ1-KCNE1 K Channel Complexes. ,
PLoS ONE
 4 , e4236 , 2009
Cells
Oocytes
Enzymes
Collagenase : 0.2%
Medium
see reference
Reproductive
Frog
Xenopus laevis, female
Pannaccione Anna, Castaldo Pasqualina, Ficker Eckhard, Annunziato Lucio, Taglialatela Maurizio.
Histidines 578 and 587 in the S5-S6 linker of the human Ether-a-gogo Related Gene-1 K+ channels confer sensitivity to reactive oxygen species ,
J Biol Chem
 277 , 8912-9 , 2002
Cells
Oocytes
Enzymes
Medium
see reference
Reproductive
Frog
Xenopus laevis, female
Alagem, N. , Dvir, M. and Reuveny, E.
Mechanism of Ba2+ Block of a Mouse Inwardly Rectifying K+ Channel: Differential Contribution by Two Discrete Residues ,
J Physiol
 534 (2) , 381 , 2001
Cells
Oocytes
Enzymes
Medium
CF Medium
Reproductive
Frog
Xenopus
Tian, J. , Kim, S. , Heilig, E. and Ruderman, J.
Identification of XPR-1, A Progesterone Receptor Required for Xenopus Oocyte Activation ,
Proc Natl Acad Sci U S A
 97 , 14358 , 2000
Cells
Oocytes
Enzymes
Medium
(see reference)
Reproductive
Frog
Xenopus laevis, female
Karkanias, N. and Papke, R.
Subtype-Specific Effects of Lithium on Glutamate Receptor Function ,
J Neurophysiol
 81 , 1506 , 1999
Cells
Oocytes
Enzymes
Collagenase : 0.1%
Medium
Barth's solution, CF
Reproductive
Frog
Xenopus laevis, mature female
Moriarty, T. , Gillo, B. , Carty, D. , Premont, R. , Landau, E. and Iyengar, R.
Beta gamma Subunits of GTP-Binding Proteins Inhibit Muscarinic Receptor Stimulation of Phospholipase C ,
Proc Natl Acad Sci U S A
 85 , 8865 , 1988
Cells
Oocytes
Enzymes
Collagenase : 0.2%
Medium
CF Medium
Reproductive
Hamster
Hamster, Chinese
Wallis, R. and Drickamer, K.
Molecular Determinants of Oligomer Formation and Complement Fixation in Mannose-Binding Proteins ,
J Biol Chem
 274 (6) , 3580 , 1999
Cells
Ovary
Enzymes
Trypsin : 5%
Medium
Dialyzed fetal calf serum, 10% and 0.5M Methotrexate
Reproductive
Human
Human
Chen, J. and Roan, N.
Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts. ,
Bio Protoc
 5 , 2015
Cells
Endometrial epithelial and stromal fibroblasts
Enzymes
Hyaluronidase : 62 u/ml
Medium
DMEM
Reproductive
Human
Human
Evans, J. and Salamonsen, L.
Decidualized Human Endometrial Stromal Cells are Sensors of Hormone Withdrawal in the Menstrual Inflammatory Cascade. ,
Biol Reprod
 90 , 14 , 2014
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.